FlyBase Allele Report: Dmel\robo2[X123]

General Information

Symbol

Dmel\robo2^X123

Species

D. melanogaster

Name

FlyBase ID

FBal0123720

Feature type

allele

Associated gene

Dmel\robo2

Associated Insertion(s)

Carried in Construct

Also Known As

robo2¹²³

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | embryonic stage (with robo2^x135)

(Howard et al., 2021)

Phenotype Manifest In

cell body & serotonin neuron

(Couch et al., 2004)

dorsal vessel wall cell

(Santiago-Martinez et al., 2006)

embryonic pericardial cell

(Santiago-Martinez et al., 2006)

larval longitudinal connective | embryonic stage (with robo2^x135)

(Howard et al., 2021)

serotonin neuron & ventral nerve cord

(Couch et al., 2004)

Detailed Description

Statement

Reference

robo2^X123/robo2^x135 embryos have a mildly disorganised acon scaffold in the central nervous system, but do not show defects in crossing of the midline by EW neurons.

(Evans et al., 2015)

lea^X123 embryos show much milder defects compared to robo⁴, lea^X123 double mutant embryos: 29% of lea^X123 embryos have one or more pericardial cells that migrate too far dorsally and are found closer to the dorsal midline in front of the rows of cardioblasts. lea^X123 embryos also occasionally display alignment defects in the rows of cardioblasts, although gaps between cardioblasts are not apparent.

(Santiago-Martinez et al., 2006)

Ventral nerve cords from stage 16 lea^X123/lea^X123 embryos show a random loss of ability to take up serotonin in approximately half of hemisegments. The cell bodies of serotonergic neurons in the ventral nerve cord of stage 16 lea^X123/lea^X123 embryos are shifted towards the midline compared to wild-type.

(Couch et al., 2004)

Mutant embryos do not show defects in the position of the vmd1a neuron of the ventral multidendritic neuron cluster in the abdomen; a single vmd1a neuron is seen in each hemisegment.

(Orgogozo et al., 2004)

Homozygous larvae have no detectable defect in distal cell neuron positioning in the visual system.

(Tayler et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

robo2^X123/robo2^x135 is an enhancer of abnormal neuroanatomy phenotype of fra³/fra⁴

(Evans et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^Mef2.PR, robo2^UAS.cSa, robo2^X123 has dorsal vessel wall cell | ectopic phenotype, enhanceable by robo1⁴

(Santiago-Martinez et al., 2006)

NOT Enhanced by

Statement

Reference

robo2^X123 has serotonin neuron & ventral nerve cord phenotype, non-enhanceable by eg^Mz360/eg^Mz360

(Couch et al., 2004)

Suppressed by

Statement

Reference

robo2^X123 has serotonin neuron & ventral nerve cord phenotype, suppressible by eg^UAS.cDa/Scer\GAL4^eg-Mz360

(Couch et al., 2004)

Enhancer of

Statement

Reference

robo2^X123/robo2^x135 is an enhancer of symmetrical commissure phenotype of fra³/fra⁴

(Evans et al., 2015)

robo2^X123/robo2^x135 is an enhancer of larval EW neuron phenotype of fra³/fra⁴

(Evans et al., 2015)

NOT Enhancer of

Statement

Reference

robo2^X123/robo2^X123 is a non-enhancer of serotonin neuron & ventral nerve cord phenotype of eg^Mz360

(Couch et al., 2004)

Other

Statement

Reference

robo1¹, robo2^X123 has germinal proliferation center | embryonic stage 17 phenotype

(Anllo and DiNardo, 2022)

Scer\GAL4^Mef2.PR, robo1⁴, robo2^UAS.cSa, robo2^X123 has dorsal vessel wall cell phenotype

(Santiago-Martinez et al., 2006)

robo1⁴, robo2^X123 has dorsal vessel wall cell phenotype

(Santiago-Martinez et al., 2006)

robo1⁴, robo2^X123 has embryonic pericardial cell phenotype

(Santiago-Martinez et al., 2006)

robo1¹, robo2^X123 has muscle cell of A1-7 ventral longitudinal muscle 3 phenotype

(Kramer et al., 2001, Kramer et al., 2001)

robo1¹, robo2^X123 has muscle cell of A1-7 ventral longitudinal muscle 4 phenotype

(Kramer et al., 2001, Kramer et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

In robo1¹, robo2^X123 double mutant stage 17 embryos, the testes often exhibit dispersed aggregates of germline niche cells, unlike the single anterior niche position in controls.

(Anllo and DiNardo, 2022)

robo2^X123/robo2^x135 strongly enhances the midline crossing defects seen in fra³/fra⁴ embryos: approximately 70% of EW neurons fail to cross the midline in the double mutant embryos.

(Evans et al., 2015)

Embryos mutant for both robo⁴ and lea^X123 show defects in cardioblast and pericardial cell positioning and alignment, similar to slit² embryos. The number of cardioblast nuclei that reach the dorsal midline in these embryos is approximately 86.2, compared to approximately 104 in wild type embryos.

Embryos expressing lea^Scer\UAS.cSa under the control of Scer\GAL4^Mef2.PR in a robo⁴, lea^X123 double mutant background display gaps in rows of cardioblasts indicating a loss of adhesion between cardioblasts (as seen in robo⁴, lea^X123 double mutants) as well as the mispositioning of these cells away from the dorsal midline (as seen in lea^Scer\UAS.cSa, Scer\GAL4^Mef2.PR embryos).

Embryos expressing lea^Scer\UAS.cSa under the control of Scer\GAL4^Mef2.PR in a robo⁴, lea^X123 double mutant background exhibit mispositioning of cardioblasts away from the dorsal midline which is more severe as compared to expression of lea^Scer\UAS.cSa using Scer\GAL4^Mef2.PR in an otherwise wild type background; cardioblasts are displaced even more laterally, with cardioblasts and pericardial cells even being observed in somatic muscle territory.

(Santiago-Martinez et al., 2006)

In the ventral nerve cords of stage 16 lea^X123/lea^X123 embryos, the number of hemisegments showing loss of ability to take up serotonin is not significantly increased by eg^Mz360/eg^Mz360. The loss of serotonin uptake activity and the serotonergic axon guidance phenotypes of lea^X123/lea^X123 embryos are suppressed by eg^Scer\UAS.cDa; Scer\GAL4^eg-Mz360.

(Couch et al., 2004)

robo¹ lea^X123 double mutant embryos show defects in the position of the vmd1a neuron of the ventral multidendritic neuron cluster in the abdomen; in 21% of segments loss of the vmd1a neuron in a hemisegment is associated with duplication of the vmd1a neuron in the contralateral hemisegment. In addition, in 36% of segments, some vmd1a neurons are missing from the ventral vmd clusters (in most of these segments, the missing md neurons are probably located near the midline).

(Orgogozo et al., 2004)

87% of segments show crossing of the midline by muscles 6/7 in robo¹ lea^X123 double mutant embryos. This defect is rescued by expression of robo^Scer\UAS.cKa under the control of Scer\GAL4^how-24B, but the axonal phenotype of these embryos is mutant. 1% of segments show crossing of the midline by muscles 6/7, 5% show abnormal insertion of muscle 5 and 3% show abnormal insertion of muscles 6/7 in sli² lea^X123 double mutant embryos. 15% of segments show crossing of the midline by muscles 6/7, 31% show abnormal insertion of muscle 5 and 16% show abnormal insertion of muscles 6/7 in sli² robo¹ lea^X123 triple mutant embryos.

(Kramer et al., 2001)

Muscles 6 and 7 cross the midline in most segments in robo¹ lea^X123 double mutant embryos.

(Kramer et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

lea^X123

robo2¹²³

(Howard et al., 2021, Evans et al., 2015, Santiago et al., 2014)

robo2^X123

(Anllo and DiNardo, 2022, Santiago-Martinez et al., 2006, Orgogozo et al., 2004, Tayler et al., 2004, Kramer et al., 2001)

robo2^x123

(Chaudhari et al., 2021, Santiago and Bashaw, 2017, Evans et al., 2015, Evans and Bashaw, 2012, Evans and Bashaw, 2010)

Name Synonyms

Secondary FlyBase IDs

References (14)

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