Allele Dmel\key¹

General Information
Symbol	Dmel\key¹	Species	D. melanogaster
Name		FlyBase ID	FBal0123807
Feature type	allele	Associated gene	Dmel\key
Also Known As	IKK^key1, kenny¹, DmIkkγ^key1

Map ( GBrowse )
Allele class
Mutagen
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen
Mutations Mapped to the Genome

Type Location Additional Notes References sequence variant 2R:20,673,862..20,673,866 comment=A 5 bp deletion, causing a frameshift and early translation termination halfway through the coding sequence. evidence=experimental
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference 5bp deletion starting at position 698, resulting in a frame shift which introduces a stop codon halfway through the coding region. (Rutschmann et al., 2000)
Cytology
Phenotypic Data
Phenotypic Class
	immune response defective (Gottar et al., 2002, Rutschmann et al., 2000, Ligoxygakis et al., 2002, Ryu et al., 2006, Kim et al., 2006, Bischoff et al., 2004, Aymeric et al., 2010, Bischoff et al., 2006, Berkey et al., 2009) immune response defective (with Df(2R)Dll-MP) (Rutschmann et al., 2000) viable (Rutschmann et al., 2000)
Phenotype Manifest In

Detailed Description
	Statement Reference Mutant flies are sensitive to injection with E. coli and all flies die within 40 hours of infection. (Aymeric et al., 2010) Homozygous flies are resistant to V. cholerae infection compared to controls. Compared to controls, homozygous flies are more resistant to infection with a V. cholerae ΔctxB mutant. The susceptibility of homozygous mutants to infection by V. cholerae by septic injury is not significantly different to controls. (Berkey et al., 2009) key[1] mutant flies show a very high bacterial load and a strong susceptibility to E. cloacae or E. coli infection. (Bischoff et al., 2006) key¹ flies exhibit a dramatically decreased viability when pricked with a needle dipped in a concentrated solution of gram-negative Esherichia coli or Erwinia carotovora. (Kim et al., 2006) High mortality levels are observed when key¹ flies are fed on the ROS-resistant KNU53775 yeast strain but are not observed when they are fed on a standard yeast strain (W303). Wild-type flies do not show the same sensitivity to the KNU53775 strain. (Ryu et al., 2006) Mutant flies show reduced survival compared to wild-type controls after infection with E. coli. (Bischoff et al., 2004) key¹ flies are susceptible to infection by Gram-negative bacteria. (Ligoxygakis et al., 2002) Mutant flies show significantly lower resistance to E.coli infections than wild-type flies. Mutant flies show the same level of resistance to B.bassiana (when their cuticles are coated with spores) as wild-type flies. (Rutschmann et al., 2000)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference key¹ is an enhancer of visible \| adult stage phenotype of PGRP-LF^{dsRNA.1.Scer\UAS}, Scer\GAL4^en.PU (Maillet et al., 2008)
Suppressor of
Statement Reference key¹ is a suppressor of partially lethal - majority die phenotype of PGRP-LF²⁰⁰ (Maillet et al., 2008)
NOT Suppressor of
Statement Reference key¹ is a non-suppressor of visible \| adult stage phenotype of PGRP-LF²⁰⁰ (Maillet et al., 2008)
Other
Statement Reference Dif¹, key¹ has immune response defective \| recessive \| adult stage phenotype (Brown et al., 2009)
Phenotype Manifest In
Enhancer of
Statement Reference key¹ is an enhancer of wing phenotype of PGRP-LF^{dsRNA.1.Scer\UAS}, Scer\GAL4^en.PU (Maillet et al., 2008)
NOT Suppressor of
Statement Reference key¹ is a non-suppressor of wing phenotype of PGRP-LF²⁰⁰ (Maillet et al., 2008)
Additional Comments
Genetic Interactions
Statement Reference Short term starvation does not improve the survival of Dif[1]-key[1] double mutants following infection with Gram-negative bacteria. (Brown et al., 2009) key[1] does not suppress the notching phenotypes and low adult viability seen in homozygous PGRP-LF[200] mutants. A key[1] background suppresses the wing notches seen when PGRP-LF[dsRNA.1.Scer\UAS] is expressed under the control of Scer\GAL4[en.PU]. (Maillet et al., 2008) Dif¹ key¹ double mutants die at the same rate as Dif¹ mutants when their cuticles are coated with spores of B.bassiana. (Rutschmann et al., 2000)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer
	Arose as: possible cluster with key², key³, key⁵, key⁶, key⁷, key⁸, key⁹ and key¹⁰ - the identical mutation found in these alleles may have been present in the starting stock used in the mutagenesis screen. (Rutschmann et al., 2000)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 8 )
Reported As
Symbol Synonym	DmIkkγ^key1 (Khush et al., 2002) DmIKKγ^key1 (Vidal et al., 2001) IKK^key1 (Bischoff et al., 2004, Maillet et al., 2008, Bischoff et al., 2006) IKKγ (Maillet et al., 2008) kenny¹ (Pili-Floury et al., 2004, Georgel et al., 2001) key1 (Ligoxygakis et al., 2002) key¹ (Gottar et al., 2006, Castillejo-Lopez and Haecker, 2005, Delaney et al., 2006, Ryu et al., 2006, Mulinari et al., 2006, Kim et al., 2006, Leclerc et al., 2006, Berkey et al., 2009, Wang et al., 2012) Key¹ (Bergeret et al., 2008, Aymeric et al., 2010)
Name Synonym
Secondary FlyBase IDs

References ( 26 )

Generate a list of	All references relating to this allele ( 26 )

List References by type	Research paper ( 26 )
Recent research papers ( 1 )
	Wang et al., 2012, J. Immunol. 188(8): 3993--4000 The Drosophila protein mustard tailors the innate immune response activated by the immune deficiency pathway. [FBrf0217978] Show all research papers ...

Allele Dmel\key1

Allele Dmel\key¹