Allele Dmel\comt^clp

General Information
Symbol	Dmel\comt^clp	Species	D. melanogaster
Name		FlyBase ID	FBal0123921
Feature type	allele	Associated gene	Dmel\comt
Also Known As	comt^x

Map ( GBrowse )
Allele class	loss of function allele
Mutagen
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	loss of function allele (Golby et al., 2001)
Mutagen
Mutations Mapped to the Genome

Type Location Additional Notes References deletion X:13,155,784..13,155,784 comment=Frameshift at amino acid 709, caused by a one-nucleotide deletion of the first base of the codon. The resulting polypeptide has the carboxy-terminal 37 amino-acids replaced by 18 novel amino acids. evidence=experimental (Golby et al., 2001, Littleton et al., 2001)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Frameshift at amino acid 709, caused by a one-nucleotide deletion. Resulting polypeptide would have the usual carboxy-terminal 37 amino-acids replaced by 18 novel amino acids. (Golby et al., 2001) Contains a single bp deletion that shifts the reading frame 36 amino acids upstream of the carboxy terminus and leads to a premature stop codon. (Littleton et al., 2001)
Cytology
Phenotypic Data
Phenotypic Class
	lethal (with Df(1)C246) (Sanyal and Krishnan, 2001) lethal (with Df(1)wy26) (Sanyal and Krishnan, 2001) lethal \| germline clone \| pharate adult stage \| recessive (Golby et al., 2001) lethal \| larval stage \| recessive (Sanyal and Krishnan, 2001) lethal \| pharate adult stage \| recessive (Golby et al., 2001) lethal \| recessive (Littleton et al., 2001) neuroanatomy defective \| larval stage (Sanyal and Krishnan, 2001) paralytic \| heat sensitive (with comt¹) (Sanyal and Krishnan, 2001) paralytic \| heat sensitive (with comt⁴) (Sanyal and Krishnan, 2001) paralytic \| heat sensitive (with comt⁶) (Sanyal and Krishnan, 2001) uncoordinated \| adult stage \| recessive (Golby et al., 2001)
Phenotype Manifest In
	NMJ bouton (Sanyal and Krishnan, 2001)
Detailed Description
	Statement Reference When dissected from the pupal case the flies appear normal but are incapable of coordinated movement. The lethal phase is not altered in embryos derived from germ-line clones for comt^clp. Hemizygous male larvae take much longer than wild type to roll from lying on their dorsal surface to their ventral surface. They are often found protruding vertically from the surface of the medium for long periods of time and burrow deeply and die - possibly as a result of suffocation. Hemizygous males also accumulate gas in their intestinal tract. (Golby et al., 2001) Some homozygous escapers are found; these animals appear as normal adults, but do not eclose from the pupal case, and if freed from the case show seizure-like behaviour. Electroretinograms of freed adult escapers are normal. Mutant larvae have a reduced number of boutons per muscle area compared to controls (assayed at the neuromuscular junction of muscles 6 and 7 in the A2 segment). (Sanyal and Krishnan, 2001)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference comt^clp has lethal \| recessive \| pharate adult stage phenotype, suppressible by Scer\GAL4^elav-C155/Nsf2^Scer\UAS.cGa (Golby et al., 2001)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	comt^clp is rescued by comt^hs.PP (Sanyal and Krishnan, 2001) comt^clp is rescued by Scer\GAL4^elav-C155/comt^Scer\UAS.cGa (Golby et al., 2001)
Not rescued by	comt^clp is not rescued by comt^Scer\UAS.cGa/Scer\GAL4^C57 (Golby et al., 2001) comt^clp is not rescued by comt^Scer\UAS.cGa/Scer\GAL4^Mef2.PR (Golby et al., 2001) comt^clp is not rescued by comt^Scer\UAS.cGa/Scer\GAL4^Mhc.PW (Golby et al., 2001) comt^clp is not rescued by Scer\GAL4^how-24B/comt^Scer\UAS.cGa/Scer\GAL4^how-24B (Golby et al., 2001)
Comments
Stocks ( 1 )
Bloomington	27890 comt^clp/FM7a
Notes on Origin
Discoverer	K.S. Krishnan

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	comt^CEX (Sanyal, 2009.7.28) comt^CLP (Littleton et al., 2001, ) comt^clp (Sanyal, 2009.7.28, Popodi et al., 2010-) comt^x (Sanyal, 2009.7.28, Sanyal and Krishnan, 2001) dNSF1^clp (Golby et al., 2001)
Name Synonym
Secondary FlyBase IDs
	FBal0128023
References ( 5 )
Research paper	Golby et al., 2001, Genetics 158(1): 265--278 Partitioning of N-ethylmaleimide-sensitive fusion (NSF) protein function in Drosophila melanogaster. Dnsf1 is required in the nervous system, and dnsf2 is required in mesoderm. [FBrf0135807] Littleton et al., 2001, Proc. Natl. Acad. Sci. U.S.A. 98(21): 12233--12238 SNARE-complex disassembly by NSF follows synaptic-vesicle fusion. [FBrf0139793] Sanyal and Krishnan, 2001, Neuroreport 12(7): 1363--1366 Lethal comatose mutation in Drosophila reveals possible role for NSF in neurogenesis. [FBrf0137072]
Personal communication to FlyBase	Popodi et al., 2010-, Small X duplications for the stock center collection. Small X duplications for the stock center collection. [FBrf0210621] Sanyal, 2009.7.28, comt[clp] synonyms. comt[clp] synonyms. [FBrf0208735]

Allele Dmel\comtclp

Allele Dmel\comt^clp