Allele Dmel\Tsc1^Scer\UAS.cTa

General Information
Symbol	Dmel\Tsc1^Scer\UAS.cTa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Tapon	FlyBase ID	FBal0123961
Feature type	allele	Associated gene	Dmel\Tsc1

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Tapon et al., 2001)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of a Tsc1 cDNA. (Tapon et al., 2001)
Carried in construct	P{UAS-Tsc1.T} (Stocker et al., 2003, Radimerski et al., 2002, Murthy et al., 2004, Tapon et al., 2001, Hennig et al., 2006, Kockel et al., 2010, Chang and Neufeld, 2009, Chang and Neufeld, 2009, Lee et al., 2006, Slaidina et al., 2009, Juhász et al., 2008, Bland et al., 2010, Khurana et al., 2006, Scott et al., 2004, Scott et al., 2004, Colombani et al., 2003, Lam et al., 2010, Montagne et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In
	fat body, with Scer\GAL4^Act5C.PP (Scott et al., 2004)
Detailed Description
	Statement Reference Activation of autophagy by Tsc1/gig overexpression can non-autonomously suppress starvation-induced autophagy in neighboring cells. (Scott et al., 2004) Expression of Tsc1^Scer\UAS.cTa in the eye under the control of Scer\GAL4^ey.PH has no effect. Expression of Tsc1^Scer\UAS.cTa in the posterior compartment of the wing under the control of Scer\GAL4^en-e16E has no effect on cell or compartment size. (Tapon et al., 2001)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Scer\GAL4^ey.PH, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has visible phenotype, enhanceable by Cdk4³ (Tapon et al., 2001) Scer\GAL4^ey.PH, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has visible phenotype, enhanceable by CycA⁰³⁹⁴⁶ (Tapon et al., 2001) Scer\GAL4^ey.PH, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has visible phenotype, enhanceable by CycE⁰⁵²⁰⁶ (Tapon et al., 2001) Scer\GAL4^ey.PH, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has visible phenotype, enhanceable by Ras85D^e1B (Tapon et al., 2001)
NOT Enhanced by
Statement Reference Scer\GAL4^Act5C.PP, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has decreased cell size \| somatic clone phenotype, non-enhanceable by Pi3K59F^Δm22 (Juhász et al., 2008)
Suppressed by
Statement Reference CycD^Scer\UAS.cDa, Scer\GAL4^ey.PH, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has visible phenotype, suppressible by Cdk4^Scer\UAS.cMa, Scer\GAL4^ey.PH (Tapon et al., 2001) Scer\GAL4^ey.PH, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has visible phenotype, suppressible by CycE^Scer\UAS.cLa, Scer\GAL4^ey.PH (Tapon et al., 2001) Scer\GAL4^ey.PH, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has visible phenotype, suppressible by dm^Scer\UAS.cZa/Scer\GAL4^ey.PH (Tapon et al., 2001)
NOT suppressed by
Statement Reference Scer\GAL4^ey.PH, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has visible phenotype, non-suppressible by BacA\p35^Scer\UAS.cHa, Scer\GAL4^ey.PH (Tapon et al., 2001)
Suppressor of
Statement Reference gig^Scer\UAS.cTa, Scer\GAL4^e22c, Tsc1^Scer\UAS.cTa is a suppressor of increased cell death phenotype of Scer\GAL4^e22c, hppy^Scer\UAS.cLa (Lam et al., 2010)
Other
Statement Reference CycD^Scer\UAS.cDa, Scer\GAL4^ey.PH, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has visible phenotype (Tapon et al., 2001) Pi3K59F^Δm22, Scer\GAL4^Act5C.PP, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has decreased cell size \| somatic clone phenotype (Juhász et al., 2008) Scer\GAL4^Act5C.PP, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has decreased cell size \| somatic clone phenotype (Tapon et al., 2001, Tapon et al., 2001, Juhász et al., 2008) Scer\GAL4^en-e16E, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has decreased cell size phenotype (Tapon et al., 2001, Tapon et al., 2001) Scer\GAL4^en-e16E, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has visible phenotype (Tapon et al., 2001, Tapon et al., 2001) Scer\GAL4^ey.PH, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has visible phenotype (Tapon et al., 2001, Tapon et al., 2001, Murthy et al., 2004, Murthy et al., 2004) Scer\GAL4^ppl.PP, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has developmental rate defective phenotype (Colombani et al., 2003)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^ey.PH, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has eye phenotype, enhanceable by Cdk4³ (Tapon et al., 2001) Scer\GAL4^ey.PH, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has eye phenotype, enhanceable by CycA⁰³⁹⁴⁶ (Tapon et al., 2001) Scer\GAL4^ey.PH, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has eye phenotype, enhanceable by CycE⁰⁵²⁰⁶ (Tapon et al., 2001) Scer\GAL4^ey.PH, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has eye phenotype, enhanceable by Ras85D^e1B (Tapon et al., 2001)
Suppressed by
Statement Reference CycD^Scer\UAS.cDa, Scer\GAL4^ey.PH, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has eye phenotype, suppressible by Cdk4^Scer\UAS.cMa, Scer\GAL4^ey.PH (Tapon et al., 2001) Scer\GAL4^Act5C.PP, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has lysosome \| somatic clone phenotype, suppressible by Pi3K59F^Δm22 (Juhász et al., 2008) Scer\GAL4^ey.PH, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has eye phenotype, suppressible by CycE^Scer\UAS.cLa, Scer\GAL4^ey.PH (Tapon et al., 2001) Scer\GAL4^ey.PH, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has eye phenotype, suppressible by dm^Scer\UAS.cZa/Scer\GAL4^ey.PH (Tapon et al., 2001) Scer\GAL4^ey.PH, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has eye phenotype, suppressible by Hr46^EY05451, Scer\GAL4^ey.PH (Montagne et al., 2010) Scer\GAL4^ey.PH, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has eye phenotype, suppressible by Hr46^EY10268, Scer\GAL4^ey.PH (Montagne et al., 2010) Scer\GAL4^ey.PH, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has eye phenotype, suppressible by S6k^Scer\UAS.cMa, Scer\GAL4^ey.PH (Montagne et al., 2010)
NOT suppressed by
Statement Reference Scer\GAL4^Act5C.PP, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has fat body \| somatic clone phenotype, non-suppressible by Pi3K59F^Δm22 (Juhász et al., 2008) Scer\GAL4^Act5C.PP, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has wing disc phenotype, non-suppressible by Scer\GAL4^ey.PH/BacA\p35^Scer\UAS.cHa (Tapon et al., 2001) Scer\GAL4^ey.PH, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has eye phenotype, non-suppressible by BacA\p35^Scer\UAS.cHa, Scer\GAL4^ey.PH (Tapon et al., 2001)
Suppressor of
Statement Reference gig^Scer\UAS.cTa, gig^Scer\UAS.cTa, Tsc1^Scer\UAS.cTa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of Rheb^EP50.084, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Stocker et al., 2003) gig^Scer\UAS.cTa, Scer\GAL4^e22c, Tsc1^Scer\UAS.cTa is a suppressor of wing margin bristle phenotype of Scer\GAL4^e22c, hppy^Scer\UAS.cLa (Lam et al., 2010) gig^Scer\UAS.cTa, Scer\GAL4^e22c, Tsc1^Scer\UAS.cTa is a suppressor of wing phenotype of Scer\GAL4^e22c, hppy^Scer\UAS.cLa (Lam et al., 2010) gig^Scer\UAS.cTa, Scer\GAL4^e22c, Tsc1^Scer\UAS.cTa is a suppressor of wing vein phenotype of Scer\GAL4^e22c, hppy^Scer\UAS.cLa (Lam et al., 2010)
Other
Statement Reference Scer\GAL4^Act5C.PP, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has dorsal mesothoracic disc & mitotic cell cycle \| somatic clone phenotype (Tapon et al., 2001, Tapon et al., 2001) Scer\GAL4^Act5C.PP, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has fat body \| somatic clone phenotype (Juhász et al., 2008) Scer\GAL4^Act5C.PP, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has lysosome \| somatic clone phenotype (Juhász et al., 2008) Scer\GAL4^Cg.PA, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa has embryonic/larval neuroblast phenotype (Sousa-Nunes et al., 2011) Scer\GAL4^ey.PH, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has eye disc phenotype (Tapon et al., 2001, Tapon et al., 2001) Scer\GAL4^ey.PH, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has eye phenotype (Tapon et al., 2001, Tapon et al., 2001, Murthy et al., 2004, Murthy et al., 2004) Scer\GAL4^ey.PH, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has wing \| posterior compartment phenotype (Tapon et al., 2001, Tapon et al., 2001) Scer\GAL4^ey.PH, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa has eye phenotype (Montagne et al., 2010, Montagne et al., 2010) Scer\GAL4^nab, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa has embryonic/larval neuroblast phenotype (Sousa-Nunes et al., 2011) Scer\GAL4^ppl.PP, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has embryonic/larval fat body phenotype (Colombani et al., 2003) Scer\GAL4^ppl.PP, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has lipid droplet & larval oenocyte phenotype (Gutierrez et al., 2007) Scer\GAL4^repo.PU, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa has embryonic/larval neuroblast phenotype (Sousa-Nunes et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference Central nervous system neuroblasts of fed larvae co-expressing gig[Scer\UAS.cTa] and Tsc1[Scer\UAS.cTa] under the control of either Scer\GAL4[Cg.PA] or Scer\GAL4[nab] show a reduction in EdU incorporation compared to controls. Central nervous system neuroblasts of fed second instar larvae co-expressing gig[Scer\UAS.cTa] and Tsc1[Scer\UAS.cTa] under the control of Scer\GAL4[n-syb.PS] show no significant difference in EdU incorporation compared to controls. Central nervous system neuroblasts of fed second instar larvae co-expressing gig[Scer\UAS.cTa] and Tsc1[Scer\UAS.cTa] under the control of Scer\GAL4[repo.PU] show a reduction in EdU incorporation compared to controls. (Sousa-Nunes et al., 2011) Coexpression of Tsc1[Scer\UAS.cTa] and gig[Scer\UAS.cTa] under the control of Scer\GAL4[arm.PS] rescues the aberrant heart phenotypes normally caused by a high-fat diet. Coexpression of Tsc1[Scer\UAS.cTa] and gig[Scer\UAS.cTa] under the control of Scer\GAL4[Lsp2.PH] rescues the increase in triglyceride levels and the heart defects normally caused by a high-fat diet. Coexpression of Tsc1[Scer\UAS.cTa] and gig[Scer\UAS.cTa] under the simultaneous control of both Scer\GAL4[Scer\UAS.cHa] and Scer\GAL4[tin.cBa] does not rescue the increase in triglyceride levels caused by a high-fat diet. The heart defects that are normally caused by a high-fat diet are also not robustly rescued in these animals. (Birse et al., 2010) Co-expression of Tsc1[Scer\UAS.cTa] and gig[Scer\UAS.cTa] (together forming the tuberous sclerosis complex) under the control of Scer\GAL4[e22c] does not affect cell death in the posterior compartment of the wing discs seen upon expression of hppy[Scer\UAS.cLa]. (Lam et al., 2010) Ectopic expression of Tsc1[Scer\UAS.cTa] and gig[Scer\UAS.cTa] under the control of Scer\GAL4[ey.PH] suppresses the growth of the developing eye. Co-expression of S6k[Scer\UAS.cMa] suppresses the reduction in growth of the developing eye seen upon expression of Tsc1[Scer\UAS.cTa] and gig[Scer\UAS.cTa] under the control of Scer\GAL4[ey.PH]. Ectopic expression of Hr46[EY05451], combined with Tsc1[Scer\UAS.cTa] and gig[Scer\UAS.cTa], in the developing eye under the control of Scer\GAL4[ey.PH], largely counteracts the growth-suppressive effects of Tsc1 and gig. Ectopic expression of Hr46[EY10268], combined with Tsc1[Scer\UAS.cTa] and gig[Scer\UAS.cTa], in the developing eye under the control of Scer\GAL4[ey.PH], largely counteracts the growth-suppressive effects of Tsc1 and gig. (Montagne et al., 2010) Expression of Tsc1[Scer\UAS.cTa] and gig[Scer\UAS.cTa] under the control of Scer\GAL4[Act5C.PP] results in a significant reduction in fat-body cell size. This reduction in cell size is also observed in a Pi3K59F[Δm22] background. Co-overexpression of Tsc1[Scer\UAS.cTa] and gig[Scer\UAS.cTa] in fat-body cells under the control of Scer\GAL4[Act5C.PP] induces the formation of autolysosomes in fed conditions. Over-expression in a Pi3K59F[Δm22] mutant animal does not result in the induction of autolysosome formation. (Juhász et al., 2008) Co-expression of Tsc1^Scer\UAS.cTa and gig^Scer\UAS.cTa under the control of Scer\GAL4^ppl.PP results in a marked accumulation of large lipid droplets in the oenocytes of 100% of fed larvae (this contrasts with wild-type larvae, where oenocytes accumulate lipid droplets specifically under starvation conditions). (Gutierrez et al., 2007) Overexpression of Tsc1[Scer\UAS.cTa] and gig[Scer\UAS.cTa] in a specific subset of cells within the wing imaginal discs (under the control of Scer\GAL4[Scer\FRT.Act5C] decreases the clone size compared to wild-type controls. This overexpression results in a dramatic reduction in wing marginal bristle size. Overexpression of Tsc1[Scer\UAS.cTa] and gig[Scer\UAS.cTa] in a specific subset of cells within the wing imaginal discs (under the control of Scer\GAL4[Bx-MS1096] decreases the clone size compared to wild-type controls. This overexpression results in a dramatic reduction in wing marginal bristle size. (Lee et al., 2006) Co-expression of gig^Scer\UAS.cTa and Tsc1^Scer\UAS.cTa under the control of Scer\GAL4^ey.PH results in flies with smaller eyes than normal. This small eye phenotype is enhanced if the flies are also mutant for one of hiw^ND8, hiw^EP1305, hiw^ND42, hiw^ND69 or hiw^EMS. (Murthy et al., 2004) When Tsc1[Scer\UAS.cTa] and gig[Scer\UAS.cTa] are driven together by Scer\GAL4[ppl.PP] in the fat body, vesicle aggregation and restricted endoreduplication is seen in the fat body and a growth defect is also seen. (Colombani et al., 2003) Ommatidial size in Scer\GAL4^GMR.PF/+; Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/+ flies is reduced to 0.68 of wild-type. The large size and disorganisation of ommatidia in Scer\GAL4^GMR.PF/+; Rheb^EP50.084/+ flies is largely suppressed by Tsc1^Scer\UAS.cTa with gig^Scer\UAS.cTa (ommatidial are = 0.95 of wild-type size and are only slightly disorganised. (Stocker et al., 2003) Expression of both Tsc1^Scer\UAS.cTa and gig^Scer\UAS.cTa in the eye under the control of Scer\GAL4^ey.PH results in a much smaller eye than normal. Coexpression of BacA\p35^Scer\UAS.cHa does not alter this phenotype. Coexpression of both CycD^Scer\UAS.cDa and Cdk4^Scer\UAS.cMa fully suppresses the phenotype. Coexpression of CycD^Scer\UAS.cDa partially suppresses the phenotype. Coexpression of CycE^Scer\UAS.cLa fully suppresses the phenotype. Coexpression of dm^Scer\UAS.cZa or S6k^Scer\UAS.cWa suppresses the phenotype. Addition of one copy of Cdk4³, CycE⁰⁵²⁰⁶, CycA⁰³⁹⁴⁶, Ras85D^e1B enhances the phenotype. Coexpression of Pten^Scer\UAS.cGb enhances the phenotype. Expression of both Tsc1^Scer\UAS.cTa and gig^Scer\UAS.cTa in the developing eye under the control of Scer\GAL4^GMR.PF reduces the growth of postmitotic cells. Expression of both Tsc1^Scer\UAS.cTa and gig^Scer\UAS.cTa in the posterior compartment of the wing under the control of Scer\GAL4^en-e16E reduces its size by approximately 2-fold. Cell density in the posterior compartment is approximately 60% higher than in control wings. Expression of both Tsc1^Scer\UAS.cTa and gig^Scer\UAS.cTa in clones in the wing imaginal disc under the control of Scer\GAL4^Act5C.PP results in a reduction in the size of cycling cells. These cells also show an increase in the population of cells in G1 at the expense of those in S phase. The proportion of cells in G2 is unchanged. All phases of the cell cycle are lengthened; G1 is lengthened by 75%, G2 by 47% and S phase by 12%. Expression of both Tsc1^Scer\UAS.cTa and gig^Scer\UAS.cTa in the wing imaginal disc under the control of Scer\GAL4^Act5C.PP results in clones that are smaller than control clones. Coexpression of BacA\p35^Scer\UAS.cHa does not alter this phenotype. (Tapon et al., 2001)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Tsc1^Scer\UAS.cTa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Tapon (Tapon et al., 2001)
Secondary FlyBase IDs

References ( 21 )

Generate a list of	All references relating to this allele ( 21 )

List References by type	Research paper ( 18 ) Supplementary material ( 2 ) Letter ( 1 )
Recent research papers ( 1 )
	Sousa-Nunes et al., 2011, Nature 471(7339): 508--512 Fat cells reactivate quiescent neuroblasts via TOR and glial insulin relays in Drosophila. [FBrf0214426] Show all research papers ...

Allele Dmel\Tsc1Scer\UAS.cTa

Allele Dmel\Tsc1^Scer\UAS.cTa