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General Information
Symbol
Dmel\Tsc1UAS.cTa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Tapon
FlyBase ID
FBal0123961
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Tsc1
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a Tsc1 cDNA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 1 )
 

The amelioration of the phenotype in Npc1aKK101077 expressing flies is observed upon simultaneous co-expression of both Tsc1Scer\UAS.cTa and gigScer\UAS.cTa. sf161013

Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Activation of autophagy by Tsc1/gig overexpression can non-autonomously suppress starvation-induced autophagy in neighboring cells.

Expression of Tsc1Scer\UAS.cTa in the eye under the control of Scer\GAL4ey.PH has no effect. Expression of Tsc1Scer\UAS.cTa in the posterior compartment of the wing under the control of Scer\GAL4en-e16E has no effect on cell or compartment size.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT Enhanced by
Suppressed by
NOT suppressed by
Suppressor of
NOT Suppressor of
Other
Statement
Reference
Phenotype Manifest In
Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Suppressor of
NOT Suppressor of
Other
Statement
Reference

Scer\GAL4ppl.PP, Tsc1UAS.cTa, gigUAS.cTa/gigScer\UAS.cTa has lipid droplet & larval oenocyte phenotype

Scer\GAL4Act5C.PP, Tsc1UAS.cTa, gigUAS.cTa/gigScer\UAS.cTa has dorsal mesothoracic disc & mitotic cell cycle | somatic clone phenotype

Additional Comments
Genetic Interactions
Statement
Reference

Adult females simultaneously expressing Tsc1Scer\UAS.cTa and gigScer\UAS.cTa under the control of Scer\GAL4fit.PS show significantly weaker suppression of feeding (on standard food) after pre-feeding with tryptone compared to controls.

Co-expression of Tsc1Scer\UAS.cTa and gigScer\UAS.cTa under the control of Scer\GAL4phm.PO (using tub-Gal80ts to limit the time of expression to late L2/L3 larval stages) or the Scer\GAL4P0206 driver results in an almost complete loss of lipid droplets from the prothoracic gland cells in third instar larvae.

The accumulation of lipid droplets observed in prothoracic gland cells of third instar larvae expressing Npc1aKK101077 under the control of either Scer\GAL4phm.PO or Scer\GAL4P0206 is prevented by co-expression of Tsc1Scer\UAS.cTa and gigScer\UAS.cTa.

Oenocytes of larvae expressing both Tsc1Scer\UAS.cTa and gigScer\UAS.cTa under the control of the Scer\GAL4Adh.PF driver accumulate lipid droplets regardless of their fed/starved status.

Co-expression of Tsc1Scer\UAS.cTa and gigScer\UAS.cTa suppresses the enlarged bouton phenotype seen when Vap-33AP58S.cRa.Scer\UAS is expressed under the control of Scer\GAL4elav-C155.

Co-expression of Tsc1Scer\UAS.cTa and gigScer\UAS.cTa does not suppress the small bouton phenotype seen when Vap-33AScer\UAS.cRa is expressed under the control of Scer\GAL4elav-C155.

Co-expression of Tsc1Scer\UAS.cTa and gigScer\UAS.cTa has no effect on bouton size.

Reduction of TORC1 through expression of Tsc1Scer\UAS.cTa and gigScer\UAS.cTa (under the control of Scer\GAL4GMR.PU) suppresses the overgrowth of Pten117 mutant tissue.

Scer\GAL4Myo31DF-NP0001-mediated co-expression of Tsc1Scer\UAS.cTa and gigScer\UAS.cTa results in smaller midguts at 0h relative puparium formation (RPF) compared to controls. These midguts appear to undergo rapid degradation and are particularly fragile.

The midgut phenotype seen in Tsc1Scer\UAS.cTa, gigScer\UAS.cTa Scer\GAL4Myo31DF-NP0001 animals is suppressed by co-expression of Atg1GL00047.

The midgut phenotype seen in Tsc1Scer\UAS.cTa, gigScer\UAS.cTa Scer\GAL4Myo31DF-NP0001 animals is suppressed by co-expression of Atg18VDRC.cUa.

Central nervous system neuroblasts of fed larvae co-expressing gigScer\UAS.cTa and Tsc1Scer\UAS.cTa under the control of either Scer\GAL4Cg.PA or Scer\GAL4nab show a reduction in EdU incorporation compared to controls.

Central nervous system neuroblasts of fed second instar larvae co-expressing gigScer\UAS.cTa and Tsc1Scer\UAS.cTa under the control of Scer\GAL4n-syb.PS show no significant difference in EdU incorporation compared to controls.

Central nervous system neuroblasts of fed second instar larvae co-expressing gigScer\UAS.cTa and Tsc1Scer\UAS.cTa under the control of Scer\GAL4repo.PU show a reduction in EdU incorporation compared to controls.

Coexpression of Tsc1Scer\UAS.cTa and gigScer\UAS.cTa under the control of Scer\GAL4arm.PS rescues the aberrant heart phenotypes normally caused by a high-fat diet.

Coexpression of Tsc1Scer\UAS.cTa and gigScer\UAS.cTa under the control of Scer\GAL4Lsp2.PH rescues the increase in triglyceride levels and the heart defects normally caused by a high-fat diet.

Coexpression of Tsc1Scer\UAS.cTa and gigScer\UAS.cTa under the simultaneous control of both Scer\GAL4Scer\UAS.cHa and Scer\GAL4tin.cBa does not rescue the increase in triglyceride levels caused by a high-fat diet. The heart defects that are normally caused by a high-fat diet are also not robustly rescued in these animals.

Co-expression of Tsc1Scer\UAS.cTa and gigScer\UAS.cTa (together forming the tuberous sclerosis complex) under the control of Scer\GAL4e22c does not affect cell death in the posterior compartment of the wing discs seen upon expression of hppyScer\UAS.cLa.

Ectopic expression of Tsc1Scer\UAS.cTa and gigScer\UAS.cTa under the control of Scer\GAL4ey.PH suppresses the growth of the developing eye.

Co-expression of S6kScer\UAS.cMa suppresses the reduction in growth of the developing eye seen upon expression of Tsc1Scer\UAS.cTa and gigScer\UAS.cTa under the control of Scer\GAL4ey.PH.

Ectopic expression of Hr46EY05451, combined with Tsc1Scer\UAS.cTa and gigScer\UAS.cTa, in the developing eye under the control of Scer\GAL4ey.PH, largely counteracts the growth-suppressive effects of Tsc1 and gig.

Ectopic expression of Hr46EY10268, combined with Tsc1Scer\UAS.cTa and gigScer\UAS.cTa, in the developing eye under the control of Scer\GAL4ey.PH, largely counteracts the growth-suppressive effects of Tsc1 and gig.

Expression of Tsc1Scer\UAS.cTa and gigScer\UAS.cTa under the control of Scer\GAL4Act5C.PP results in a significant reduction in fat-body cell size. This reduction in cell size is also observed in a Pi3K59FΔm22 background.

Co-overexpression of Tsc1Scer\UAS.cTa and gigScer\UAS.cTa in fat-body cells under the control of Scer\GAL4Act5C.PP induces the formation of autolysosomes in fed conditions. Over-expression in a Pi3K59FΔm22 mutant animal does not result in the induction of autolysosome formation.

Co-expression of Tsc1Scer\UAS.cTa and gigScer\UAS.cTa under the control of Scer\GAL4ppl.PP results in a marked accumulation of large lipid droplets in the oenocytes of 100% of fed larvae (this contrasts with wild-type larvae, where oenocytes accumulate lipid droplets specifically under starvation conditions).

Overexpression of Tsc1Scer\UAS.cTa and gigScer\UAS.cTa in a specific subset of cells within the wing imaginal discs (under the control of Scer\GAL4Scer\FRT.Act5C decreases the clone size compared to wild-type controls. This overexpression results in a dramatic reduction in wing marginal bristle size.

Overexpression of Tsc1Scer\UAS.cTa and gigScer\UAS.cTa in a specific subset of cells within the wing imaginal discs (under the control of Scer\GAL4Bx-MS1096 decreases the clone size compared to wild-type controls. This overexpression results in a dramatic reduction in wing marginal bristle size.

Co-expression of gigScer\UAS.cTa and Tsc1Scer\UAS.cTa under the control of Scer\GAL4ey.PH results in flies with smaller eyes than normal. This small eye phenotype is enhanced if the flies are also mutant for one of hiwND8, hiwEP1305, hiwND42, hiwND69 or hiwEMS.

When Tsc1Scer\UAS.cTa and gigScer\UAS.cTa are driven together by Scer\GAL4ppl.PP in the fat body, vesicle aggregation and restricted endoreduplication is seen in the fat body and a growth defect is also seen.

Ommatidial size in Scer\GAL4GMR.PF/+; Tsc1Scer\UAS.cTa, gigScer\UAS.cTa/+ flies is reduced to 0.68 of wild-type. The large size and disorganisation of ommatidia in Scer\GAL4GMR.PF/+; RhebEP50.084/+ flies is largely suppressed by Tsc1Scer\UAS.cTa with gigScer\UAS.cTa (ommatidial are = 0.95 of wild-type size and are only slightly disorganised.

Expression of both Tsc1Scer\UAS.cTa and gigScer\UAS.cTa in the eye under the control of Scer\GAL4ey.PH results in a much smaller eye than normal. Coexpression of BacA\p35Scer\UAS.cHa does not alter this phenotype. Coexpression of both CycDScer\UAS.cDa and Cdk4Scer\UAS.cMa fully suppresses the phenotype. Coexpression of CycDScer\UAS.cDa partially suppresses the phenotype. Coexpression of CycEScer\UAS.cLa fully suppresses the phenotype. Coexpression of dmScer\UAS.cZa or S6kScer\UAS.cWa suppresses the phenotype. Addition of one copy of Cdk43, CycE05206, CycA03946, Ras85De1B enhances the phenotype. Coexpression of PtenScer\UAS.cGb enhances the phenotype. Expression of both Tsc1Scer\UAS.cTa and gigScer\UAS.cTa in the developing eye under the control of Scer\GAL4GMR.PF reduces the growth of postmitotic cells. Expression of both Tsc1Scer\UAS.cTa and gigScer\UAS.cTa in the posterior compartment of the wing under the control of Scer\GAL4en-e16E reduces its size by approximately 2-fold. Cell density in the posterior compartment is approximately 60% higher than in control wings. Expression of both Tsc1Scer\UAS.cTa and gigScer\UAS.cTa in clones in the wing imaginal disc under the control of Scer\GAL4Act5C.PP results in a reduction in the size of cycling cells. These cells also show an increase in the population of cells in G1 at the expense of those in S phase. The proportion of cells in G2 is unchanged. All phases of the cell cycle are lengthened; G1 is lengthened by 75%, G2 by 47% and S phase by 12%. Expression of both Tsc1Scer\UAS.cTa and gigScer\UAS.cTa in the wing imaginal disc under the control of Scer\GAL4Act5C.PP results in clones that are smaller than control clones. Coexpression of BacA\p35Scer\UAS.cHa does not alter this phenotype.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
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Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Tsc1Scer\UAS.cTa
Tsc1UAS.cTa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Tapon
Secondary FlyBase IDs
    References (30)