Allele Dmel\TotA^Scer\UAS.cEa

General Information
Symbol	Dmel\TotA^Scer\UAS.cEa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Ekengren	FlyBase ID	FBal0123971
Feature type	allele	Associated gene	Dmel\TotA

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Ekengren et al., 2001)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS sequences drive expression of TotA. (Ekengren et al., 2001)
Carried in construct	P{UAS-TotA.E} (Ekengren et al., 2001, Ekengren et al., 2001, Mahapatra et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	chemical resistant, with Scer\GAL4^c754 (Mahapatra et al., 2010) chemical resistant, with Scer\GAL4^elav-C155 (Mahapatra et al., 2010) lethal \| larval stage, with Scer\GAL4^Act5C.PI (Ekengren et al., 2001)
Phenotype Manifest In

Detailed Description
	Statement Reference Expression of TotA[Scer\UAS.cEa] under the control of Scer\GAL4[elav-C155] induces MeHg tolerance. However, it does not affect the rate of eclosion in a MeHg environment. A significant fraction of these larvae progress to a late stage of pupal development as determined by formation of dark pupae. Extracting these pupae from their cases reveals that normal metamorphosis has occurred (as determined by the presence of complete adult eyes, wings, and bristle structures. In contrast, controls show a marked deficit in development, with 'white' undeveloped pupae. Expression of TotA[Scer\UAS.cEa] in the fat body of the larvae, under the control of Scer\GAL4[c754], confers tolerance to MeHg, as observed through the greater number of dark pupae that result at the 10-20υM Melts Treatments. (Mahapatra et al., 2010) When TotA^Scer\UAS.cEa is driven by Scer\GAL4^c729 flies become more resistant to heat stress. Flies survive significantly longer at 37^oC and are significantly more active during times of heat stress. (Ekengren et al., 2001) Flies expressing TotA^Scer\UAS.cEa under the control of Scer\GAL4^hs.PB survive significantly longer than control siblings in the presence of paraquat, however, the increased resistance may be caused by differences in genetic background rather than overexpression of TotA. (Ekengren et al., 2001)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	Tot^Scer\UAS.cEa TotA^Scer\UAS.cEa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Ekengren (Ekengren et al., 2001)
Secondary FlyBase IDs

References ( 3 )
Research paper	Mahapatra et al., 2010, Toxicol. Sci. 116(1): 225--238 Identification of methylmercury tolerance gene candidates in Drosophila. [FBrf0211073] Ekengren et al., 2001, Curr. Biol. 11(9): 714--718 A humoral stress response in Drosophila. [FBrf0136837] Ekengren et al., 2001, Curr. Biol. 11(18): 1479 A humoral stress response in Drosophila. [FBrf0138269]

Allele Dmel\TotAScer\UAS.cEa

Allele Dmel\TotA^Scer\UAS.cEa