FlyBase Allele Report: Dmel\Tak1[K46R.M.UAS]

General Information

Symbol

Dmel\Tak1^K46R.M.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0123981

Feature type

allele

Associated gene

Dmel\Tak1

Associated Insertion(s)

Carried in Construct

P{UAS-Tak1.K46R.M}

Also Known As

UAS-TAK1^DN, UAS-Tak1^K46R

Key Links

Transgenic product class

dominant_negative_variant

(Mihaly et al., 2001)

missense_variant

(Mihaly et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Mihaly et al., 2001)

missense_variant

(Mihaly et al., 2001)

Mutagen

in vitro construct

(Mihaly et al., 2001)

Progenitor genotype

Carried in construct

P{UAS-Tak1.K46R.M}

Cytology

Description

UASt regulatory sequences drive expression of a dominant-negative, kinase dead form of Tak1 (carries the amino acid replacement K46R).

(Mihaly et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Tak1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates lower respiratory tract disease

modeled by PGRP-LC^x.UAS

(Wagner et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response, with Scer\GAL4^da.G32

(Stronach et al., 2014)

abnormal immune response, with Tak1²

(Stronach et al., 2014)

wild-type, with Scer\GAL4^arm.PS

(Mihaly et al., 2001)

wild-type, with Scer\GAL4^en-e16E

(Mihaly et al., 2001)

Phenotype Manifest In

mesonotum, with Scer\GAL4^ap-md544

(Mihaly et al., 2001)

mesonotum, with Scer\GAL4^pnr-MD237

(Mihaly et al., 2001)

microchaeta, with Scer\GAL4^ap-md544

(Mihaly et al., 2001)

microchaeta, with Scer\GAL4^pnr-MD237

(Mihaly et al., 2001)

Detailed Description

Statement

Reference

The number of synaptic boutons at neuromuscular junctions in larvae expressing Scer\GAL4^nSyb.PS>Tak1^{K46R.M.Scer\UAS} is similar to that of wild-type.

(West et al., 2015)

Expression of Tak1^{K46R.M.Scer\UAS} under the control of Scer\GAL4^da.G32 modestly increases susceptibility of Tak1²/+ females to E.coli.

(Stronach et al., 2014)

Expression of Tak1^{K46R.Scer\UAS} when driven by Scer\GAL4^en-e16E, Scer\GAL4^pnr-MD237, or Scer\GAL4^arm.PS, does not significantly affect development in the embryo. Expression of Tak1^{K46R.Scer\UAS} when driven by Scer\GAL4^pnr-MD237 or Scer\GAL4^ap-md544, produces adults that display incomplete closure of the thorax, giving rise to a mild thoracic cleft at the dorsal midline of the notum, and also a lack of bristles in the area. This phenotype has a high penetrance (about 91%).

(Mihaly et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Tak1^K46R.M.UAS/Scer\GAL4^arm.PS is an enhancer of partially lethal - majority die phenotype of slpr^BS06

(Polaski et al., 2006)

Suppressor of

Statement

Reference

Tak1^K46R.M.UAS/Scer\GAL4^nSyb.PS is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Rab8¹/Rab8^B229

(West et al., 2015)

Tak1^K46R.M.UAS/Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Scer\GAL4^GMR.PF, egr^GS9830

(Stronach et al., 2014, Polaski et al., 2006)

Tak1^K46R.M.UAS, Scer\GAL4^dpp.blk1 is a suppressor of visible phenotype of Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1, puc^E69/puc[+]

(Neisch et al., 2010)

Tak1^K46R.M.UAS, Scer\GAL4^Switch1.106 is a suppressor of abnormal immune response phenotype of PGRP-LE^UAS.Tag:FLAG, Scer\GAL4^Switch1.106

(Libert et al., 2006)

Phenotype Manifest In

Suppressor of

Statement

Reference

Tak1^K46R.M.UAS/Scer\GAL4^nSyb.PS is a suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Rab8¹/Rab8^B229

(West et al., 2015)

Tak1^K46R.M.UAS/Scer\GAL4^nSyb.PS is a suppressor of NMJ bouton | third instar larval stage phenotype of Rab8¹/Rab8^B229

(West et al., 2015)

Tak1^K46R.M.UAS/Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, egr^GS9830

(Stronach et al., 2014, Polaski et al., 2006)

Tak1^K46R.M.UAS, Scer\GAL4^dpp.blk1 is a suppressor of wing blade phenotype of Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1, puc^E69/puc[+]

(Neisch et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Tak1^{K46R.M.Scer\UAS} suppresses the reduction in eye size seen when egr^GS9830 is expressed under the control of Scer\GAL4^GMR.PF.

(Stronach et al., 2014)

Co-expression of Tak1^{K46R.M.Scer\UAS} suppresses the Scer\GAL4^dpp.blk1>Moe^{dsRNA.327-775.Scer\UAS}; puc^E69/+ wing blade phenotype.

(Neisch et al., 2010)

Simultaneous expression of Tak1^{K46R.M.Scer\UAS} driven by Scer\GAL4^S106-GS significantly suppresses the increased pathogen resistance phenotype of PGRP-LE^{Scer\UAS.T:Zzzz\FLAG}-expressing flies.

(Libert et al., 2006)

Expression of Tak1^{K46R.M.Scer\UAS} under the control of Scer\GAL4^arm.PS significantly increases the percentage of embryonic lethality and severity of cuticle defects of embryos derived from slpr^BS06/+ females.

The eye ablation phenotype seen in adults expressing egr^GS9830 under the control of Scer\GAL4^GMR.PF is almost completely suppressed by coexpression of Tak1^{K46R.M.Scer\UAS} under the control of Scer\GAL4^GMR.PF.

(Polaski et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

58811

w^*; P{UAS-Tak1.K46R.M}T4/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Tak1^{K46R.M.Scer\UAS}

Tak1^K46R.M.UAS

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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