FB2025_01 , released February 20, 2025
Allele: Dmel\Pdk1EP3091
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General Information
Symbol
Dmel\Pdk1EP3091
Species
D. melanogaster
Name
FlyBase ID
FBal0124093
Feature type
allele
Associated gene
Dmel\Pdk1
Associated Insertion(s)
P{EP}Pdk1EP3091
Carried in Construct
Also Known As
EP(3)3091
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
P-element activity
(Cho et al., 2001, Gene Disruption Project members, 2001-, Rintelen et al., 2001)
Progenitor genotype
Associated Insertion(s)
P{EP}Pdk1EP3091
Cytology
Description

The P{EP} insertion is in the fourth intron of Pk61C.

(Cho et al., 2001)

P{EP} insertion 710bp upstream of the 5' end of exon 3. P{EP} element is inserted so that expression from the Scer\UAS sequences in the element drives expression of antisense Pk61C.

(Rintelen et al., 2001)
Allele components
Component
Use(s)
Inserted element
P{EP}
Regulatory region(s)
UAS
Mutations Mapped to the Genome
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      exacerbates  tauopathy
      modeled by Hsap\MAPTUAS.cWa
      (Feuillette et al., 2020)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      When raised on food containing 6% ethanol, Pdk1EP3091 homozygotes take a longer time to eclose but show complete suppression of the reduced survival, compared to wild type.

      (Logan-Garbisch et al., 2014)

      Expression of Pk61CEP3091 under the control of Scer\GAL4ptc-559.1 (the animals are initially raised at 18oC and then shifted to 29oC after 10 days) results in a reduced number of early germ cells (stem cells, gonialblasts and spermatogonia) in the testis.

      (Schulz et al., 2004)

      Homozygotes produce no ventral cuticles and do not develop into the larval stage. Apoptotic activity is higher than controls in these embryos.

      (Cho et al., 2001)

      Homozygous and transheterozygous Pk61C5/Pk61CEP3091 larvae die during the second instar.

      (Rintelen et al., 2001)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Statement
      Reference

      Pdk1EP3091 has increased cell death phenotype, suppressible by AktUAS.Tag:HA/Scer\GAL4hs.PB

      (Cho et al., 2001)
      Enhancer of
      Suppressor of
      Statement
      Reference

      Pdk14ΔEP/Pdk1EP3091, Scer\GAL4GMR.PF is a suppressor of visible phenotype of InRUAS.cHa, Scer\GAL4GMR.PF

      (Rintelen et al., 2001)
      Phenotype Manifest In
      Enhancer of
      Statement
      Reference

      Pdk1[+]/Pdk1EP3091 is an enhancer of eye phenotype of Hsap\MAPTUAS.cWa, Scer\GAL4GMR.PF

      (Feuillette et al., 2020)

      Pdk14ΔEP/Pdk1EP3091, Scer\GAL4GMR.PF is an enhancer of eye phenotype of InRUAS.cHa, Scer\GAL4GMR.PF

      (Rintelen et al., 2001)
      Suppressor of
      Statement
      Reference

      Pdk14ΔEP/Pdk1EP3091, Scer\GAL4GMR.PF is a suppressor of eye phenotype of InRUAS.cHa, Scer\GAL4GMR.PF

      (Rintelen et al., 2001)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Eye size in animals expressing InRScer\UAS.cHa under the control of Scer\GAL4GMR.PF is restored almost to wild type by Pk61CEP3091/Pk61C4ΔEP, although eye roughness is increased.

      (Rintelen et al., 2001)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to complement

      Pdk12

      (Cho et al., 2001)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Excision of the P{EP} element can revert the lethal phenotype of Pk61CEP3091.

      (Rintelen et al., 2001)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      PDK-11
      (Chan et al., 2007)
      Pdk1EP3091
      (Feuillette et al., 2020, Logan-Garbisch et al., 2014)
      Pk61CEP3091
      dPDK11
      (Rintelen et al., 2001)
      dPDK1
      (Cho et al., 2001)
      Name Synonyms
      Secondary FlyBase IDs
        References (7)