Allele Dmel\Pc^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG}

General Information
Symbol	Dmel\Pc^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG}	Species	D. melanogaster
Name		FlyBase ID	FBal0124097
Feature type	allele	Associated gene	Dmel\Pc

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Roseman et al., 2001) in vitro construct - regulatory fusion (Roseman et al., 2001)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Expression of full length cDNA of Pc fused to T:Zzzz\FLAG and T:ZnF-su(Hw) is driven by a heat shock promoter. (Roseman et al., 2001)
Carried in construct	P{ZnF-Pc.R} (Roseman et al., 2001)
Tagged with	T:Zzzz\FLAG (Roseman et al., 2001)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| heat sensitive (Roseman et al., 2001)
Phenotype Manifest In

Detailed Description
	Statement Reference Early (embryogenesis) heat shock induction of Pc^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG} cause lethality. (Roseman et al., 2001)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Pc^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG}, su(Hw)⁷/su(Hw)^E8, w^BR.E.BR has eye color defective phenotype, suppressible by ph-p⁴⁰⁹ (Roseman et al., 2001) Pc^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG}, su(Hw)^V/su(Hw)^E8, w^BR.E.BR has eye color defective phenotype, suppressible by ph-p⁴⁰⁹ (Roseman et al., 2001)
Enhancer of
Statement Reference Pc^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG} is an enhancer of body color defective phenotype of su(Hw)⁷/su(Hw)^E8, y^+mDint2 (Roseman et al., 2001) Pc^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG} is an enhancer of body color defective phenotype of su(Hw)^V/su(Hw)^E8, y^+mDint2 (Roseman et al., 2001) Pc^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG} is an enhancer of eye color defective phenotype of su(Hw)⁷/su(Hw)^E8, w^BR.BR (Roseman et al., 2001) Pc^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG} is an enhancer of eye color defective phenotype of su(Hw)⁷/su(Hw)^E8, w^BR.E.BR (Roseman et al., 2001) Pc^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG} is an enhancer of eye color defective phenotype of su(Hw)^V/su(Hw)^E8, w^BR.BR (Roseman et al., 2001) Pc^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG} is an enhancer of eye color defective phenotype of su(Hw)^V/su(Hw)^E8, w^BR.E.BR (Roseman et al., 2001)
Phenotype Manifest In
Suppressed by
Statement Reference Pc^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG}, su(Hw)⁷/su(Hw)^E8, w^BR.E.BR has pigment cell phenotype, suppressible by ph-p⁴⁰⁹ (Roseman et al., 2001) Pc^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG}, su(Hw)^V/su(Hw)^E8, w^BR.E.BR has pigment cell phenotype, suppressible by ph-p⁴⁰⁹ (Roseman et al., 2001)
Enhancer of
Statement Reference Pc^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG}, su(Hw)⁷/su(Hw)^E8 is an enhancer of adult cuticle phenotype of su(Hw)⁷/su(Hw)^E8, y^+mDint2 (Roseman et al., 2001) Pc^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG} is an enhancer of adult cuticle phenotype of su(Hw)^V/su(Hw)^E8, y^+mDint2 (Roseman et al., 2001) Pc^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG} is an enhancer of pigment cell phenotype of su(Hw)⁷/su(Hw)^E8, w^BR.BR (Roseman et al., 2001) Pc^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG} is an enhancer of pigment cell phenotype of su(Hw)⁷/su(Hw)^E8, w^BR.E.BR (Roseman et al., 2001) Pc^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG} is an enhancer of pigment cell phenotype of su(Hw)^V/su(Hw)^E8, w^BR.BR (Roseman et al., 2001) Pc^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG} is an enhancer of pigment cell phenotype of su(Hw)^V/su(Hw)^E8, w^BR.E.BR (Roseman et al., 2001)
Additional Comments
Genetic Interactions
Statement Reference Expression of w^BR.BR and w^BR.E.BR is reduced (in a background of reduced su(Hw)⁺) by the expression of tethered Pc. This effect is seen without the use of heat shock. Expression of y^+mDint2 is reduced (in a background of reduced su(Hw)⁺) by the expression of tethered Pc, Scm or esc. In the case of Pc^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG}, the effect is seen without the use of heat shock. Expression in the body cuticle is less sensitive to repression than in the wing cuticle. ph-p⁴⁰⁹ completely suppresses the strong silencing of w^BR.E.BR in P{SUPor-P}22F caused by tethered Pc. (Roseman et al., 2001)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Pc^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG} Pc^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Roseman et al., 2001, Genetics 158(1): 291--307 Long-range repression by multiple Polycomb Group (PcG) proteins targeted by fusion to a defined DNA-binding domain in Drosophila. [FBrf0135809]

Allele Dmel\Pchs.T:ZnF-su(Hw),T:Zzzz\FLAG

Allele Dmel\Pc^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG}