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General Information
Symbol
Dmel\Nsf2EQ.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0124104
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-dNSF2E/Q, UAS-NSF2E/Q
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS sequences drive expression of the mutated form of Nsf2 (carries the amino acid replacement E326Q).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Nsf2EQ.Scer\UAS under the control of Scer\GAL4elav.PLu results in a hypersprouting phenotype at the larval neuromuscular junction.

Expression of Nsf2EQ.Scer\UAS, under the control of Scer\GAL4BG487, leads to impaired exocytosis of Fas2 in larval MNMJs.

Expression of Nsf2EQ.Scer\UAS under the control of Scer\GAL4elav.PLu results in an expansion of the nerve terminal at the larval neuromuscular junction (NMJ), which is most apparent as a lengthening and elaboration of the branching pattern. The total branch length of the mutant NMJs is about 60% longer than that of controls. In approximately 10% of hemisegments inappropriate branching and misrouting of axons to some NMJs is seen. This is most apparent for the NMJs of muscles 12 and 13. Multiple sites of innervation are seen on both muscle 12 and muscle 13 in larvae expressing Nsf2EQ.Scer\UAS under the control of Scer\GAL4elav.PLu (in contrast to wild type which have a single point of innervation for each muscle) and in addition, axons sometimes "reach back" from muscle 12 to innervate muscle 13. Rarely, axons that innervate muscle 12 pass under muscle 13, rather than over it, on their way to innervate muscle 12.

The amplitude of the evoked EJC at the neuromuscular junction is reduced in larvae expressing Nsf2EQ.Scer\UAS under the control of Scer\GAL4elav.PLu or Scer\GAL4elav-C155 compared to wild type. The evoked EJC amplitude of the mutant animals shows reduced calcium cooperativity compared to wild type.

The amplitude of the spontaneous mEJC at the neuromuscular junction is not significantly different from wild type in larvae expressing Nsf2EQ.Scer\UAS under the control of Scer\GAL4elav-C155. However, there is a marked reduction in mEJC frequency in the mutant animals compared to wild type.

Larvae expressing Nsf2EQ.Scer\UAS under the control of Scer\GAL4elav-C155 show increased fatigue of synaptic transmission; the mutant animals fatigue to an amplitude of about 45% of the first pulse under conditions of moderate continuous stimulation, whereas wild-type animals fatigue to an amplitude of about 70% of the first pulse. The rate of fatigue is faster in the mutant animals.

Animals expressing Nsf2EQ.Scer\UAS under the control of Scer\GAL4elav-C155 have fewer releasable vesicles in the synapse compared to wild type.

Expression of Nsf2EQ.Scer\UAS under the control of Scer\GAL4C96 results in mild notches in the wing margin.

When Nsf2EQ.Scer\UAS is driven by Scer\GAL4C96, a loss of wing margin is seen.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
NOT Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Other
Phenotype Manifest In
Enhanced by
Statement
Reference
NOT Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

Larvae co-expressing Nsf2EQ.Scer\UAS and Syn1NIG.7152R under the control of Scer\GAL4elav.PLu show only a weak increase in total synaptic branch length at the neuromuscular junction. These larvae show a slightly reduced frequency of peristaltic waves compared to wild type.

Larvae expressing Nsf2EQ.Scer\UAS under the control of Scer\GAL4elav.PLu in a Syn237 background show a dramatic increase in total synaptic branch length at the neuromuscular junction. These larvae show a reduced frequency of peristaltic waves compared to wild type.

Larvae co-expressing Nsf2EQ.Scer\UAS and Syn1NIG.7152R under the control of Scer\GAL4elav.PLu in a Syn237 background show a very dramatic increase in total synaptic branch length at the neuromuscular junction. These larvae show a reduced frequency of peristaltic waves compared to wild type.

Co-expression of Syx1670-329.Scer\UAS does not modify the notched wing phenotype caused by expression of Nsf2EQ.Scer\UAS under the control of Scer\GAL4C96. Co-expression of Syx1670-352.Scer\UAS enhances the notched wing phenotype caused by expression of Nsf2EQ.Scer\UAS under the control of Scer\GAL4C96.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Nsf2EQ.Scer\UAS
Nsf2EQ.UAS
Name Synonyms
Secondary FlyBase IDs
    References (7)