Allele Dmel\CadN⁴⁰⁵

General Information
Symbol	Dmel\CadN⁴⁰⁵	Species	D. melanogaster
Name		FlyBase ID	FBal0124374
Feature type	allele	Associated gene	Dmel\CadN
Also Known As	Ncad⁴⁰⁵

Allele class
Mutagen
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective (Nern et al., 2005, Biersmith et al., 2011, Petrovic and Hummel, 2008) neuroanatomy defective \| recessive \| somatic clone (Hasegawa et al., 2011) neuroanatomy defective \| somatic clone (Ting et al., 2005) optomotor response defective (Lee et al., 2001) optomotor response defective \| somatic clone (Clandinin et al., 2001, Lee et al., 2001)
Phenotype Manifest In
	adult brain \| somatic clone (Hasegawa et al., 2011) lamina plexus \| somatic clone (Lee et al., 2001) lateral longitudinal fascicle (Biersmith et al., 2011) medulla \| somatic clone (Lee et al., 2001) medulla layer M3 (Petrovic and Hummel, 2008) medulla layer M6 (Petrovic and Hummel, 2008) medullary intrinsic neuron Mi1 \| somatic clone (Hasegawa et al., 2011) photoreceptor cell R4 \| somatic clone (Lee et al., 2001) photoreceptor cell R7 (Petrovic and Hummel, 2008) photoreceptor cell R7 \| somatic clone (Ting et al., 2005, Nern et al., 2005, Lee et al., 2001) photoreceptor cell R8 \| somatic clone (Lee et al., 2001)
Detailed Description
	Statement Reference Homozygous embryos show mild central nervous system defects. (Biersmith et al., 2011) Proximal arborization is occasionally eliminated in neuron clones homozygous for CadN[405]. The defect is adult brains are diverse. (Hasegawa et al., 2011) In CadN[405] mutant homozygous clones, R7 cells terminate their axon projections in the outer M3 layer, rather than the M6 layer. (Petrovic and Hummel, 2008) At 48% after pupal formation (APF), all R cell axons homozygous for CadN⁴⁰⁵ extend aberrantly (64%), or do not extend at all (36%). (Nern et al., 2005) At 17% APF, 21% of CadN⁴⁰⁵ R7 growth cones (analysed as single cell mutant clones) fail to reach the R7-temporary layer in the medulla; the mutant axons either expand their growth cones incorrectly at the R8-temporary layer or between the R7 and R8 temporary layers. In addition, over half of the mutant growth cones show severe morphological defects. At 25% APF, 55% of the mutant R7 axons terminate at the R8 temporary layer or between the R7 and R8 temporary laters. Some of the mutant axons leave a small filopodium connecting to the R7 temporary layer. (Ting et al., 2005) Mosaic flies in which the photoreceptor cells are homozygous show defects in the optomotor response. (Clandinin et al., 2001) The ability of mutant flies to detect motion is approximately 5 times worse than wild-type. The response of mutant flies to a UV/Vis choice test is approximately 8 fold worse than wild-type. Mutants perform well, though less well than wild-type, in counter-current fast phototaxis assays. In CadN⁴⁰⁵ mosaic eyes, the R7 and R8 projections that normally form a regular array in the medulla fail to form this array and often terminate at the outer edge of the medulla. Individual R cells do not make the characteristic projections seen in the wild-type. In the optic lobe of CadN⁴⁰⁵ mosaic eyes, the lamina plexus forms irregular clumps, rather than the smooth line of uniform thickness seen in wild-type. R8 termini exhibit defects in local topographical mapping failing to form the regular array in the medulla normally seen. The array of R4 termini is also disrupted, also R4 growth cones do not expand fully within the lamina plexus. The array of R7 termini is also disrupted and their termini exhibit elongated thickening along the terminal region. However ganglion specificity is not affected and the columnar organisation of the lamina neurons is normal. Glial cells differentiate and migrate normally into the lamina, however the organisation of glial cells is disrupted such that in some regions of the lamina, they fail to form the three layers seen in wild-type. When individual R7 axons are mutant, they fail to terminate at the R7 target layer, M6, and stop instead at the R8 layer, M3. However topographic mapping of the R7 in the medulla appears normal in these animals. When homozygous somatic clones are made in the eye so that only mutant R7 cells persist, the response from these flies to a UV/Vis choice test is several times worse than wild-type. (Lee et al., 2001)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT suppressed by
Statement Reference CadN⁴⁰⁵ has neuroanatomy defective phenotype, non-suppressible by seq^Scer\UAS.cBa/Scer\GAL4^GMR.long (Petrovic and Hummel, 2008)
Enhancer of
Statement Reference CadN[+]/CadN⁴⁰⁵ is an enhancer of neuroanatomy defective \| somatic clone phenotype of Rich² (Tong et al., 2011)
NOT Enhancer of
Statement Reference CadN[+]/CadN⁴⁰⁵ is a non-enhancer of neuroanatomy defective \| somatic clone phenotype of Rich¹ (Tong et al., 2011)
Suppressor of
Statement Reference CadN⁴⁰⁵ is a suppressor of neuroanatomy defective phenotype of Scer\GAL4^GMR.long, seq^Scer\UAS.cBa (Petrovic and Hummel, 2008)
Phenotype Manifest In
NOT suppressed by
Statement Reference CadN⁴⁰⁵ has medulla layer M3 phenotype, non-suppressible by seq^Scer\UAS.cBa/Scer\GAL4^GMR.long (Petrovic and Hummel, 2008) CadN⁴⁰⁵ has medulla layer M6 phenotype, non-suppressible by seq^Scer\UAS.cBa/Scer\GAL4^GMR.long (Petrovic and Hummel, 2008) CadN⁴⁰⁵ has photoreceptor cell R7 phenotype, non-suppressible by seq^Scer\UAS.cBa/Scer\GAL4^GMR.long (Petrovic and Hummel, 2008)
Enhancer of
Statement Reference CadN[+]/CadN⁴⁰⁵ is an enhancer of photoreceptor cell R7 \| somatic clone phenotype of Rich² (Tong et al., 2011)
NOT Enhancer of
Statement Reference CadN[+]/CadN⁴⁰⁵ is a non-enhancer of photoreceptor cell R7 \| somatic clone phenotype of Rich¹ (Tong et al., 2011)
Suppressor of
Statement Reference CadN⁴⁰⁵ is a suppressor of medulla layer M3 phenotype of Scer\GAL4^GMR.long, seq^Scer\UAS.cBa (Petrovic and Hummel, 2008) CadN⁴⁰⁵ is a suppressor of medulla layer M6 phenotype of Scer\GAL4^GMR.long, seq^Scer\UAS.cBa (Petrovic and Hummel, 2008) CadN⁴⁰⁵ is a suppressor of photoreceptor cell R8 phenotype of Scer\GAL4^GMR.long, seq^Scer\UAS.cBa (Petrovic and Hummel, 2008)
Additional Comments
Genetic Interactions
Statement Reference CadN[405]/+ enhances the R7 targeting phenotype seen in Rich[2] eye clones made using the 'eyFLP' system. CadN[405]/+ does not enhance the R7 targeting phenotype seen in Rich[1] eye clones made using the 'eyFLP' system. (Tong et al., 2011) CadN[405] suppresses the R8 cell targeting defects seen when seq[Scer\UAS.cBa] is expressed under the control of Scer\GAL4[GMR.long]. The R8 axons no longer terminate in the M6 layer of the medulla and correctly target the M3 layer. However the flies do show an R7 targeting phenotype; the R7 photoreceptors target the M3 layer rather than the M6 layer, as is seen in CadN[405] mutants alone. (Petrovic and Hummel, 2008) CadN⁴⁰⁵ Lar²¹²⁷ double mutant R7 growth cones show similar phenotypes to CadN⁴⁰⁵ single mutant R7 growth cones. (Ting et al., 2005)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescued by	CadN⁴⁰⁵ is partially rescued by CadN^18A.Scer\UAS/Scer\GAL4^tub.PU (Nern et al., 2005) CadN⁴⁰⁵ is partially rescued by Scer\GAL4^elav-C155/CadN^{7b-13a-18a.Scer\UAS} (Ting et al., 2005) CadN⁴⁰⁵ is partially rescued by Scer\GAL4^elav-C155/CadN^{7b-13a-18b.Scer\UAS} (Ting et al., 2005) CadN⁴⁰⁵ is partially rescued by Scer\GAL4^elav-C155/CadN^{7b-13b-18a.Scer\UAS} (Ting et al., 2005) CadN⁴⁰⁵ is partially rescued by Scer\GAL4^elav-C155/CadN^Scer\UAS.cIa (Ting et al., 2005) CadN⁴⁰⁵ is partially rescued by Scer\GAL4^tub.PU/CadN^18B.Scer\UAS (Nern et al., 2005) CadN⁴⁰⁵ is partially rescued by Scer\GAL4^tub.PU/CadN^Scer\UAS.cIa (Nern et al., 2005)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer
	Found in a screen using a retina dependant behavioural test - an optomotor assay to assess the function of photoreceptor cells R1 to R6. (Lee et al., 2001)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	CadN⁴⁰⁵ (Tong et al., 2011, Petrovic and Hummel, 2008) Ncad1^omb405 (Biersmith et al., 2011) Ncad⁴⁰⁵ (Ting et al., 2005, Clandinin et al., 2001, Biersmith et al., 2011) N-cad⁴⁰⁵ (Hummel and Zipursky, 2004) NCad^omb405 (Hasegawa et al., 2011)
Name Synonym
Secondary FlyBase IDs

References ( 9 )
Research paper	Biersmith et al., 2011, PLoS ONE 6(1): e16120 The DOCK Protein Sponge Binds to ELMO and Functions in Drosophila Embryonic CNS Development. [FBrf0212890] Hasegawa et al., 2011, Development 138(5): 983--993 Concentric zones, cell migration and neuronal circuits in the Drosophila visual center. [FBrf0213020] Tong et al., 2011, Neuron 71(3): 447--459 Rich Regulates Target Specificity of Photoreceptor Cells and N-Cadherin Trafficking in the Drosophila Visual System via Rab6. [FBrf0214681] Petrovic and Hummel, 2008, Nature 456(7223): 800--803 Temporal identity in axonal target layer recognition. [FBrf0206960] Nern et al., 2005, Proc. Natl. Acad. Sci. U.S.A. 102(36): 12944--12949 From The Cover: an isoform-specific allele of Drosophila N-cadherin disrupts a late step of R7 targeting. [FBrf0188269] Ting et al., 2005, Development 132(5): 953--963 Drosophila N-cadherin functions in the first stage of the two-stage layer-selection process of R7 photoreceptor afferents. [FBrf0183939] Hummel and Zipursky, 2004, Neuron 42(1): 77--88 Afferent induction of olfactory glomeruli requires N-cadherin. [FBrf0175028] Clandinin et al., 2001, Neuron 32(2): 237--248 Drosophila LAR regulates R1-R6 and R7 target specificity in the visual system. [FBrf0139776] Lee et al., 2001, Neuron 30(2): 437--450 N-cadherin regulates target specificity in the Drosophila visual system. [FBrf0137065]

Allele Dmel\CadN405

Allele Dmel\CadN⁴⁰⁵