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General Information
Symbol
Dmel\AntpUAS.cMb
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Miller
FlyBase ID
FBal0124416
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Antp
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt sequences drive expression of the entire G1100 cDNA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Scer\GAL4how-24B-mediated expression of AntpScer\UAS.cMb results in an ectopic DA3 muscle in thoracic segment 1. The number of nuclei in this ectopic muscle is identical to that in wild type thoracic segments 2 and 3.

Expression of AntpScer\UAS.cMb under the control of Scer\GAL4Antp-10 results in a significant suppression of BrdU incorporation throughout the lymph gland. The zone of differentiated cells is more restricted than in wild-type, being restricted to a thin layer along the distal edge of the lymph gland.

When AntpScer\UAS.cMb is driven by Scer\GAL4sd-SG29.1 abnormal wing vein patterns and incisions in the wing margin are seen.

Stage 16 AntpScer\UAS.cMb; Scer\GAL4how-24B embryos have a normal number of cells in the dorsal vessel.

Anterior dMP2 neurons do not survive in late embryos expressing AntpScer\UAS.cMb under the control of Scer\GAL4Vap.P0201 (as occurs in wild-type embryos, where these neurons are lost by the late embryonic stage). Expression of AntpScer\UAS.cMb under the control of Scer\GAL4elav-C155 only results in a marginal increase in survival of anterior dMP2 neurons in late embryos compared to wild-type embryos (where these neurons are lost by the late embryonic stage).

In mutant embryos expressing AntpScer\UAS.cMb driven by both Scer\GAL4twi.PG and Scer\GAL4how-24B ectopic cardioblasts are seen and the lymph glands are systematically eliminated and replaced by major pericardial cells: The anterior aorta is transformed into a posterior aorta and heart tissue. This effect is not seen if AntpScer\UAS.cMb is driven by Scer\GAL4tin.CΔ4.

Expression of AntpScer\UAS.cMb under the control of Scer\GAL4sca-537.4 results in a mutant phenotype in the embryonic tritocerebrum. The phenotype has a penetrance of more than 70-80%.

Expression of AntpScer\UAS.cMb under the control of Scer\GAL4lab.PH does not result in morphological defects in the tritocerebrum or any other part of the embryonic brain.

Flies expressing AntpScer\UAS.cMb under the control of Scer\GAL4ey.PH do not eclose. Three classes of phenotype are seen; class I pharate adults lack all head structures derived from the eye-antennal discs, class II consists of eyeless flies which lack most head structures and both antennae and class III consists of eyeless animals with large parts of the head missing but one or both antennae present.

When driven by Scer\GAL4how-24B, some posterior central projections (PVs) are seen in the T1 and T2 segments suggesting T3 identity, and may even display some anterior ventral projection (AV) character in the T2 segment. Animals show an anterior shift of the first midgut constriction with reduced gastric caecum, a reduction of the first midgut chamber and an enlarged second chamber.

When driven by Scer\GAL469B, Scer\GAL4prd.RG1, or Scer\GAL4l(3)31-1-31-1 leads to a significant loss in viability.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
NOT Enhanced by
Suppressed by
NOT Enhancer of
Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

The triple co-expression of casScer\UAS.cKa, grhScer\UAS.cBa and AntpScer\UAS.cMb triggers an apparent reduction in the numbers of dividing neuroblasts and dividing neuroblast daughters in stage 12 embryos, as compared to controls.

Co-expression of casScer\UAS.cKa and AntpScer\UAS.cMb under the control of Scer\GAL4GMR42G10 results in a significant reduction in thoracic neuroblast proliferation at stage 12 (but not 14), neuroblast daughter cell proliferation at stage 14 (but not stage 12), and abdominal neuroblast and neuroblast daughter cell proliferation at both stage 12 and 14, as compared with controls.

Co-expression of E(spl)m8-HLHCK2.Scer\UAS.T:Zzzz\FLAG, casScer\UAS.cKa and AntpScer\UAS.cMb under the control of Scer\GAL4GMR42G10 enhances the reduced proliferation phenotype in neuroblast daughter cells, but does not enhance the reduced proliferation of neuroblasts, as compared with embryos expressing casScer\UAS.cKa and AntpScer\UAS.cMb under the control of Scer\GAL4GMR42G10.

Addition of AntpScer\UAS.cMb to Dfd16;Scr4 double mutant embryos (under the control of Scer\GAL4salm-459.2) results in both the Mx and Lb segments forming tracheal tubes instead of migratory gland primordia.

Co-expression of AntpScer\UAS.cMb strongly suppresses the disorganised eye phenotype caused by expression of sensScer\UAS.cNa under the control of Scer\GAL4lz-gal4.

Expression of AntpScer\UAS.cMb under the control of Scer\GAL4lab.PH rescues the tritocerebral defects seen in lab14 embryonic brains. 34.9% of embryos show a complete rescue of the defects (taking into account that the phenotypic penetrance of the lab14 phenotype is 88.6%).

Co-expression of hthScer\UAS.cPa partially suppresses the antenna to leg transformation phenotypes produced by ectopic expression of AntpScer\UAS.cMb driven by Scer\GAL4dpp.blk1. The antennae produced are aristaless and occasionally duplicated, similar to the hthScer\UAS.cPa phenotype.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
AntpScer\UAS.cKa
AntpScer\UAS.cMb
AntpUAS.cKa
AntpUAS.cMb
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Miller
Secondary FlyBase IDs
  • FBal0044641
References (28)