Allele Dmel\Antp^Scer\UAS.cMb

General Information
Symbol	Dmel\Antp^Scer\UAS.cMb	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Miller	FlyBase ID	FBal0124416
Feature type	allele	Associated gene	Dmel\Antp

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Heuer et al., 1995, Miller et al., 2001)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS sequences drive expression of the entire G1100 cDNA. (Miller et al., 2001) Construct: Antp expression is governed by Scer\UAS regulatory sequences. (Heuer et al., 1995)
Carried in construct	P{UAS-Antp.Mb} (Jiao et al., 2001, Yao et al., 1999, Ryan et al., 2005, Hirth et al., 2001, Mandal et al., 2007, Li and McGinnis, 1999, Adachi-Yamada et al., 2005, Sprecher et al., 2004, Miguel-Aliaga and Thor, 2004, Perrin et al., 2004, Miller et al., 2001, Capovilla et al., 2001, Heuer et al., 1995, Hueber et al., 2007, Anderson et al., 2011, Enriquez et al., 2010, Emmons et al., 2007, Duncan et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	lethal, with Scer\GAL4^69B (Miller et al., 2001) lethal, with Scer\GAL4^{l(3)31-1-31-1} (Miller et al., 2001) lethal, with Scer\GAL4^prd.RG1 (Miller et al., 2001) lethal \| pharate adult stage, with Scer\GAL4^ey.PH (Jiao et al., 2001) visible, with Scer\GAL4^dpp.blk1 (Yao et al., 1999) visible, with Scer\GAL4^ey.PH (Jiao et al., 2001)
Phenotype Manifest In
	adult head, with Scer\GAL4^ey.PH (Jiao et al., 2001) antenna, with Scer\GAL4^dpp.blk1 (Yao et al., 1999) antenna, with Scer\GAL4^ey.PH (Jiao et al., 2001) aorta primordium \| anterior, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG (Perrin et al., 2004) aorta primordium \| ectopic \| posterior, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG (Perrin et al., 2004) dorsal acute muscle \| ectopic, with Scer\GAL4^how-24B (Enriquez et al., 2010) embryonic/larval lymph gland, with Scer\GAL4^Antp-10 (Mandal et al., 2007) embryonic tritocerebrum, with Scer\GAL4^sca-537.4 (Sprecher et al., 2004) eye, with Scer\GAL4^ey.PH (Jiao et al., 2001) gastric caecum primordium, with Scer\GAL4^how-24B (Miller et al., 2001) metathoracic ventral acute muscle \| ectopic, with Scer\GAL4^how-24B (Miller et al., 2001) midgut chamber 1, with Scer\GAL4^how-24B (Miller et al., 2001) midgut chamber 2, with Scer\GAL4^how-24B (Miller et al., 2001) midgut constriction 1, with Scer\GAL4^how-24B (Miller et al., 2001) prothoracic ventral intersegmental muscle \| ectopic, with Scer\GAL4^how-24B (Miller et al., 2001) wing margin, with Scer\GAL4^sd-SG29.1 (Adachi-Yamada et al., 2005) wing vein, with Scer\GAL4^sd-SG29.1 (Adachi-Yamada et al., 2005)
Detailed Description
	Statement Reference Scer\GAL4[how-24B]-mediated expression of Antp[Scer\UAS.cMb] results in an ectopic DA3 muscle in thoracic segment 1. The number of nuclei in this ectopic muscle is identical to that in wild type thoracic segments 2 and 3. (Enriquez et al., 2010) Expression of Antp^Scer\UAS.cMb under the control of Scer\GAL4^Antp-10 results in a significant suppression of BrdU incorporation throughout the lymph gland. The zone of differentiated cells is more restricted than in wild-type, being restricted to a thin layer along the distal edge of the lymph gland. (Mandal et al., 2007) When Antp^Scer\UAS.cMb is driven by Scer\GAL4^sd-SG29.1 abnormal wing vein patterns and incisions in the wing margin are seen. (Adachi-Yamada et al., 2005) Stage 16 Antp^Scer\UAS.cMb; Scer\GAL4^how-24B embryos have a normal number of cells in the dorsal vessel. (Ryan et al., 2005) Anterior dMP2 neurons do not survive in late embryos expressing Antp^Scer\UAS.cMb under the control of Scer\GAL4^Vap.P0201 (as occurs in wild-type embryos, where these neurons are lost by the late embryonic stage). Expression of Antp^Scer\UAS.cMb under the control of Scer\GAL4^elav-C155 only results in a marginal increase in survival of anterior dMP2 neurons in late embryos compared to wild-type embryos (where these neurons are lost by the late embryonic stage). (Miguel-Aliaga and Thor, 2004) In mutant embryos expressing Antp^Scer\UAS.cMb driven by both Scer\GAL4^twi.PG and Scer\GAL4^how-24B ectopic cardioblasts are seen and the lymph glands are systematically eliminated and replaced by major pericardial cells: The anterior aorta is transformed into a posterior aorta and heart tissue. This effect is not seen if Antp^Scer\UAS.cMb is driven by Scer\GAL4^tin.CΔ4. (Perrin et al., 2004) Expression of Antp^Scer\UAS.cMb under the control of Scer\GAL4^sca-537.4 results in a mutant phenotype in the embryonic tritocerebrum. The phenotype has a penetrance of more than 70-80%. (Sprecher et al., 2004) Expression of Antp^Scer\UAS.cMb under the control of Scer\GAL4^lab.PH does not result in morphological defects in the tritocerebrum or any other part of the embryonic brain. (Hirth et al., 2001) Flies expressing Antp^Scer\UAS.cMb under the control of Scer\GAL4^ey.PH do not eclose. Three classes of phenotype are seen; class I pharate adults lack all head structures derived from the eye-antennal discs, class II consists of eyeless flies which lack most head structures and both antennae and class III consists of eyeless animals with large parts of the head missing but one or both antennae present. (Jiao et al., 2001) When driven by Scer\GAL4^how-24B, some posterior central projections (PVs) are seen in the T1 and T2 segments suggesting T3 identity, and may even display some anterior ventral projection (AV) character in the T2 segment. Animals show an anterior shift of the first midgut constriction with reduced gastric caecum, a reduction of the first midgut chamber and an enlarged second chamber. (Miller et al., 2001) When driven by Scer\GAL4^69B, Scer\GAL4^prd.RG1, or Scer\GAL4^{l(3)31-1-31-1} leads to a significant loss in viability. (Miller et al., 2001)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Antp^Scer\UAS.cMb, Scer\GAL4^dpp.blk1 has visible phenotype, suppressible by hth^Scer\UAS.cPa, Scer\GAL4^dpp.blk1 (Yao et al., 1999) Antp^Scer\UAS.cMb, Scer\GAL4^ey.PH has lethal \| pharate adult stage phenotype, suppressible \| partially by CycE^Scer\UAS.cLa, Scer\GAL4^ey.PH (Jiao et al., 2001) Antp^Scer\UAS.cMb, Scer\GAL4^ey.PH has visible phenotype, suppressible \| partially by CycE^Scer\UAS.cLa, Scer\GAL4^ey.PH (Jiao et al., 2001)
Suppressor of
Statement Reference Antp^Scer\UAS.cMb, Scer\GAL4^lz-gal4 is a suppressor of visible phenotype of Scer\GAL4^lz-gal4, sens^Scer\UAS.cNa (Anderson et al., 2011)
Phenotype Manifest In
Suppressed by
Statement Reference Antp^Scer\UAS.cMb, Scer\GAL4^dpp.blk1 has antenna phenotype, suppressible by hth^Scer\UAS.cPa, Scer\GAL4^dpp.blk1 (Yao et al., 1999) Antp^Scer\UAS.cMb, Scer\GAL4^ey.PH has adult head phenotype, suppressible \| partially by CycE^Scer\UAS.cLa, Scer\GAL4^ey.PH (Jiao et al., 2001)
Suppressor of
Statement Reference Antp^Scer\UAS.cMb, Scer\GAL4^lz-gal4 is a suppressor of eye phenotype of Scer\GAL4^lz-gal4, sens^Scer\UAS.cNa (Anderson et al., 2011) Scer\GAL4^lab.PH/Antp^Scer\UAS.cMb is a suppressor of embryonic tritocerebrum phenotype of lab¹⁴ (Hirth et al., 2001)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of Antp[Scer\UAS.cMb] strongly suppresses the disorganised eye phenotype caused by expression of sens[Scer\UAS.cNa] under the control of Scer\GAL4[lz-gal4]. (Anderson et al., 2011) Expression of Antp^Scer\UAS.cMb under the control of Scer\GAL4^lab.PH rescues the tritocerebral defects seen in lab¹⁴ embryonic brains. 34.9% of embryos show a complete rescue of the defects (taking into account that the phenotypic penetrance of the lab¹⁴ phenotype is 88.6%). (Hirth et al., 2001) Co-expression of hth^Scer\UAS.cPa partially suppresses the antenna to leg transformation phenotypes produced by ectopic expression of Antp^Scer\UAS.cMb driven by Scer\GAL4^dpp.blk1. The antennae produced are aristaless and occasionally duplicated, similar to the hth^Scer\UAS.cPa phenotype. (Yao et al., 1999)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	7301 w¹¹¹⁸; P{UAS-Antp.Mb}W1
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	Antp^Scer\UAS.cKa Antp^Scer\UAS.cMb Antp^UAS.cKa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Miller (Miller et al., 2001)
Secondary FlyBase IDs
	FBal0044641
References ( 19 )
Research paper	Anderson et al., 2011, Development 138(10): 1957--1966 The enhancer of trithorax and polycomb gene Caf1/p55 is essential for cell survival and patterning in Drosophila development. [FBrf0213580] Duncan et al., 2010, Dev. Biol. 347(1): 82--91 Control of the spineless antennal enhancer: Direct repression of antennal target genes by Antennapedia. [FBrf0211931] Enriquez et al., 2010, Development 137(3): 457--466 Multi-step control of muscle diversity by Hox proteins in the Drosophila embryo. [FBrf0209729] Emmons et al., 2007, Dev. Biol. 302(2): 412--426 Regulation of the Drosophila distal antennal determinant spineless. [FBrf0193220] Hueber et al., 2007, Development 134(2): 381--392 Comparative analysis of Hox downstream genes in Drosophila. [FBrf0193798] Mandal et al., 2007, Nature 446(7133): 320--324 A Hedgehog- and Antennapedia-dependent niche maintains Drosophila haematopoietic precursors. [FBrf0194088] Adachi-Yamada et al., 2005, Mol. Cell. Biol. 25(8): 3140--3150 Wing-to-leg homeosis by Spineless causes apoptosis regulated by Fish-lips, a novel leucine-rich repeat transmembrane protein. [FBrf0184157] Ryan et al., 2005, Mech. Dev. 122(9): 1023--1033 Homeotic selector genes control the patterning of seven-up expressing cells in the Drosophila dorsal vessel. [FBrf0187987] Miguel-Aliaga and Thor, 2004, Development 131(24): 6093--6105 Segment-specific prevention of pioneer neuron apoptosis by cell-autonomous, postmitotic Hox gene activity. [FBrf0180186] Perrin et al., 2004, Dev. Biol. 272(2): 419--431 Drosophila cardiac tube organogenesis requires multiple phases of Hox activity. [FBrf0179382] Sprecher et al., 2004, Mech. Dev. 121(6): 527--536 Hox gene cross-regulatory interactions in the embryonic brain of Drosophila. [FBrf0179451] Capovilla et al., 2001, Development 128(8): 1221--1230 Direct regulation of the muscle-identity gene apterous by a Hox protein in the somatic mesoderm. [FBrf0136036] Hirth et al., 2001, Development 128(23): 4781--4788 Functional equivalence of Hox gene products in the specification of the tritocerebrum during embryonic brain development of Drosophila. [FBrf0141495] Jiao et al., 2001, Development 128(17): 3307--3319 Headless flies generated by developmental pathway interference. [FBrf0138373] Miller et al., 2001, Mech. Dev. 102(1-2): 3--16 Cross-regulation of Hox genes in the Drosophila melanogaster embryo. [FBrf0135879] Miller et al., 2001, Mech. Dev. 102(1-2): 17--32 Homeotic Complex (Hox) gene regulation and homeosis in the mesoderm of the Drosophila melanogaster embryo: the roles of signal transduction and cell autonomous regulation. [FBrf0135875] Li and McGinnis, 1999, Proc. Natl. Acad. Sci. U.S.A. 96(12): 6802--6807 Activity regulation of Hox proteins, a mechanism for altering functional specificity in development and evolution. [FBrf0108881] Yao et al., 1999, Dev. Biol. 211(2): 268--276 A common mechanism for antenna-to-leg transformation in Drosophila: suppression of homothorax transcription by four HOM-C genes. [FBrf0111139] Heuer et al., 1995, Development 121(11): 3861--3876 The Drosophila homeotic target gene centrosomin, cnn, encodes a novel centrosomal protein with leucine zippers and maps to a genomic region required for midgut morphogenesis. [FBrf0084021]

Allele Dmel\AntpScer\UAS.cMb

Allele Dmel\Antp^Scer\UAS.cMb