|Name||Saccharomyces cerevisiae UAS construct a of Miller||FlyBase ID||FBal0124416|
|Feature type||allele||Associated gene||Dmel\Antp|
|Mutagen||in vitro construct - regulatory fusion|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Carried in construct|
(Jiao et al., 2001, Yao et al., 1999, Ryan et al., 2005, Hirth et al., 2001, Mandal et al., 2007, Li and McGinnis, 1999, Adachi-Yamada et al., 2005, Sprecher et al., 2004, Miguel-Aliaga and Thor, 2004, Perrin et al., 2004, Miller et al., 2001, Capovilla et al., 2001, Heuer et al., 1995, Hueber et al., 2007, Anderson et al., 2011, Enriquez et al., 2010, Emmons et al., 2007, Duncan et al., 2010)
|Phenotype Manifest In|
Scer\GAL4[how-24B]-mediated expression of Antp[Scer\UAS.cMb] results in an ectopic DA3 muscle in thoracic segment 1. The number of nuclei in this ectopic muscle is identical to that in wild type thoracic segments 2 and 3.
Expression of AntpScer\UAS.cMb under the control of Scer\GAL4Antp-10 results in a significant suppression of BrdU incorporation throughout the lymph gland. The zone of differentiated cells is more restricted than in wild-type, being restricted to a thin layer along the distal edge of the lymph gland.
When AntpScer\UAS.cMb is driven by Scer\GAL4sd-SG29.1 abnormal wing vein patterns and incisions in the wing margin are seen.
Stage 16 AntpScer\UAS.cMb; Scer\GAL4how-24B embryos have a normal number of cells in the dorsal vessel.
Anterior dMP2 neurons do not survive in late embryos expressing AntpScer\UAS.cMb under the control of Scer\GAL4Vap.P0201 (as occurs in wild-type embryos, where these neurons are lost by the late embryonic stage). Expression of AntpScer\UAS.cMb under the control of Scer\GAL4elav-C155 only results in a marginal increase in survival of anterior dMP2 neurons in late embryos compared to wild-type embryos (where these neurons are lost by the late embryonic stage).
In mutant embryos expressing AntpScer\UAS.cMb driven by both Scer\GAL4twi.PG and Scer\GAL4how-24B ectopic cardioblasts are seen and the lymph glands are systematically eliminated and replaced by major pericardial cells: The anterior aorta is transformed into a posterior aorta and heart tissue. This effect is not seen if AntpScer\UAS.cMb is driven by Scer\GAL4tin.CΔ4.
Expression of AntpScer\UAS.cMb under the control of Scer\GAL4sca-537.4 results in a mutant phenotype in the embryonic tritocerebrum. The phenotype has a penetrance of more than 70-80%.
Expression of AntpScer\UAS.cMb under the control of Scer\GAL4lab.PH does not result in morphological defects in the tritocerebrum or any other part of the embryonic brain.
Flies expressing AntpScer\UAS.cMb under the control of Scer\GAL4ey.PH do not eclose. Three classes of phenotype are seen; class I pharate adults lack all head structures derived from the eye-antennal discs, class II consists of eyeless flies which lack most head structures and both antennae and class III consists of eyeless animals with large parts of the head missing but one or both antennae present.
When driven by Scer\GAL4how-24B, some posterior central projections (PVs) are seen in the T1 and T2 segments suggesting T3 identity, and may even display some anterior ventral projection (AV) character in the T2 segment. Animals show an anterior shift of the first midgut constriction with reduced gastric caecum, a reduction of the first midgut chamber and an enlarged second chamber.
AntpScer\UAS.cMb, Scer\GAL4dpp.blk1 has visible phenotype, suppressible by hthScer\UAS.cPa, Scer\GAL4dpp.blk1
AntpScer\UAS.cMb, Scer\GAL4ey.PH has lethal | pharate adult stage phenotype, suppressible | partially by CycEScer\UAS.cLa, Scer\GAL4ey.PH
|Phenotype Manifest In|
AntpScer\UAS.cMb, Scer\GAL4dpp.blk1 has antenna phenotype, suppressible by hthScer\UAS.cPa, Scer\GAL4dpp.blk1
AntpScer\UAS.cMb, Scer\GAL4lz-gal4 is a suppressor of eye phenotype of Scer\GAL4lz-gal4, sensScer\UAS.cNa
Co-expression of Antp[Scer\UAS.cMb] strongly suppresses the disorganised eye phenotype caused by expression of sens[Scer\UAS.cNa] under the control of Scer\GAL4[lz-gal4].
Expression of AntpScer\UAS.cMb under the control of Scer\GAL4lab.PH rescues the tritocerebral defects seen in lab14 embryonic brains. 34.9% of embryos show a complete rescue of the defects (taking into account that the phenotypic penetrance of the lab14 phenotype is 88.6%).
Co-expression of hthScer\UAS.cPa partially suppresses the antenna to leg transformation phenotypes produced by ectopic expression of AntpScer\UAS.cMb driven by Scer\GAL4dpp.blk1. The antennae produced are aristaless and occasionally duplicated, similar to the hthScer\UAS.cPa phenotype.
|Complementation & Rescue Data|
|Stocks ( 1 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 4 )|
Saccharomyces cerevisiae UAS construct a of Miller
|Secondary FlyBase IDs|
|References ( 19 )|