Homozygosity for either rictorΔ24 or rictorΔ42 dramatically suppresses the enlarged eye (and enlarged ommatidium) phenotype caused by expression of Akt1Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PU.
Overexpression of Akt1Scer\UAS.T:Ivir\HA1 (under the control of Scer\GAL4Act5C.PI) using the FLP/FRT system in brain somatic clones mutant for scrib1 and wtsx1 does not cause metastatic behaviour despite accelerated tumour growth.
Expression of Akt1Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Act5C.PI in clones in the eye disc which are homozygous for scrib1 increases the amount of mutant tissue compared to scrib1 single mutant clones, but the double mutant clones do not show metastatic behaviour. Expression of Akt1Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Act5C.PI in scrib1 wtsx1 double mutant clones in the eye disc increases tumour size but does not result in metastatic behaviour.
The increase in ommatidial size seen in Akt1Scer\UAS.T:Ivir\HA1; Scer\GAL4GMR.PF flies is: unaffected by gigScer\UAS.T:Zzzz\FLAG, Tsc1Scer\UAS.T:Hsap\MYC or gigT437A.T1054A.Scer\UAS.T:Zzzz\FLAG alone; partially suppressed by gigScer\UAS.T:Zzzz\FLAG with Tsc1Scer\UAS.T:Hsap\MYC, or by gigS924A.Scer\UAS.T:Zzzz\FLAG or gigT1518A.Scer\UAS.T:Zzzz\FLAG alone; and fully suppressed by gigS924A.T1518A.Scer\UAS.T:Zzzz\FLAG alone, and by gigΔAkt-P.Scer\UAS.T:Zzzz\FLAG with or without Tsc1Scer\UAS.T:Hsap\MYC. In all these cases of full suppression, ommatidial size is actually reduced slightly compared to wild-type. The increase in wing margin bristle size seen in Akt1Scer\UAS.T:Ivir\HA1; Scer\GAL4Act5C.PI somatic clones is not suppressed by gigScer\UAS.T:Zzzz\FLAG, partially suppressed by gigScer\UAS.T:Zzzz\FLAG with Tsc1Scer\UAS.T:Hsap\MYC, and completely suppressed by gigΔAkt-P.Scer\UAS.T:Zzzz\FLAG alone, or by gigΔAkt-P.Scer\UAS.T:Zzzz\FLAG with Tsc1Scer\UAS.T:Hsap\MYC. In the latter case, the resulting bristles are significantly smaller than wild-type.
When Pk61CEP837 and Akt1Scer\UAS.T:Ivir\HA1 are coexpressed under the control of Scer\GAL4GMR.PF, the eyes display a severely crumpled morphology. The eye bristles are enlarged even more severely, and the boundaries of all ommatidia and photoreceptor cells disappear.
Animals expressing Akt1Scer\UAS.T:Ivir\HA1 and Hsap\ATX182Q.Scer\UAS (under the control of Scer\GAL4GMR.PF) have a much stronger eye phenotype than seen in either Akt1Scer\UAS.T:Ivir\HA1 or Hsap\ATX182Q.Scer\UAS on their own. Profoundly disorganised ommatidia and numerous necrotic spots are seen, as well a severely disorganised retina with missing or malformed rhabdomeres.