Allele Dmel\rl^{Sem.C.Scer\UAS}

General Information
Symbol	Dmel\rl^{Sem.C.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0124961
Feature type	allele	Associated gene	Dmel\rl

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Ciapponi et al., 2001)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS sequences drive expression of rl^Sem. (Ciapponi et al., 2001)
Carried in construct	P{UAS-rl^Sem} (Gritzan et al., 2002, Hsiao et al., 2001, Lee et al., 2003, Hipfner and Cohen, 2003, Ciapponi et al., 2001, Yue et al., 2012, Corl et al., 2009, Lee et al., 2008, Roignant and Treisman, 2010, Eddison et al., 2011, Lievens et al., 2005)
Cytology
Phenotypic Data
Phenotypic Class
	chemical resistant, with Scer\GAL4^elav-C155 (Corl et al., 2009, Eddison et al., 2011) visible, with Scer\GAL4^32B (Gritzan et al., 2002) visible, with Scer\GAL4^Bx-MS1096 (Lee et al., 2003) visible, with Scer\GAL4^GMR.PF (Corl et al., 2009)
Phenotype Manifest In
	eye, with Scer\GAL4^GMR.PF (Corl et al., 2009) wing, with Scer\GAL4^32B (Gritzan et al., 2002) wing, with Scer\GAL4^Bx-MS1096 (Lee et al., 2003) wing vein \| ectopic, with Scer\GAL4^32B (Gritzan et al., 2002, Ciapponi et al., 2001)
Detailed Description
	Statement Reference Neuronal expression of rl[Sem.C.Scer\UAS] (under the control of Scer\GAL4[elav-C155]) reduces ethanol sensitivity. Neuronal expression of rl[Sem.C.Scer\UAS] (under the control of Scer\GAL4[elav-C155]) throughout development, until the adult stage (where Scer\GAL80[ts.αTub84B] is induced) does not reduce ethanol sensitivity. Neuronal expression only after eclosion of the adult fly also fails to reduce ethanol sensitivity. (Eddison et al., 2011) Expression of rl[Sem.C.Scer\UAS] under the control of Scer\GAL4[elav-C155] results in flies with increased resistance to ethanol-induced sedation compared to control flies. Expression of rl[Sem.C.Scer\UAS] under the control of Scer\GAL4[GMR.PF] results in a rough eye phenotype. (Corl et al., 2009) 20.9% of flies expressing rl^{Sem.C.Scer\UAS} under the control of Scer\GAL4^Bx-MS1096 have blistered wings. (Lee et al., 2003) Expression of rl^{Sem.C.Scer\UAS} under the control of Scer\GAL4^32B results in ectopic vein material in the wing. The flies do not have a held out wing phenotype. (Gritzan et al., 2002) When driven by Scer\GAL4^32B, causes an extra vein phenotype which is marked at 25^oC and milder at 18^oC. (Ciapponi et al., 2001)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Statement Reference Scer\GAL4^Bx-MS1096, rl^{Sem.C.Scer\UAS} has visible phenotype, non-enhanceable by by²/by[+] (Lee et al., 2003)
Suppressed by
Statement Reference Scer\GAL4^elav-C155, rl^{Sem.C.Scer\UAS} has chemical resistant phenotype, suppressible by aru^8.128 (Eddison et al., 2011)
NOT suppressed by
Statement Reference Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, rl^{Sem.C.Scer\UAS} has visible phenotype, non-suppressible by hppy^RB.Scer\UAS/Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Corl et al., 2009)
NOT Suppressor of
Statement Reference Scer\GAL4^elav-C155/rl^{Sem.C.Scer\UAS} is a non-suppressor of chemical sensitive phenotype of aru^8.128 (Eddison et al., 2011)
Other
Statement Reference Scer\GAL4^32B, eg^18B, rl^{Sem.C.Scer\UAS} has visible phenotype (Gritzan et al., 2002, Gritzan et al., 2002)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^32B, rl^{Sem.C.Scer\UAS} has wing vein \| ectopic phenotype, enhanceable by eg^18B (Gritzan et al., 2002) Scer\GAL4^32B, rl^{Sem.C.Scer\UAS} has wing vein \| ectopic phenotype, enhanceable by Scer\GAL4^32B/kay^Scer\UAS.cEa (Ciapponi et al., 2001) Scer\GAL4^32B, rl^{Sem.C.Scer\UAS} has wing vein \| ectopic phenotype, enhanceable by svp¹ (Gritzan et al., 2002) Scer\GAL4^32B, rl^{Sem.C.Scer\UAS} has wing vein \| ectopic phenotype, enhanceable by tll¹ (Gritzan et al., 2002)
NOT Enhanced by
Statement Reference Scer\GAL4^32B, rl^{Sem.C.Scer\UAS} has wing vein \| ectopic phenotype, non-enhanceable by kni² (Gritzan et al., 2002) Scer\GAL4^Bx-MS1096, rl^{Sem.C.Scer\UAS} has wing phenotype, non-enhanceable by by²/by[+] (Lee et al., 2003)
Suppressed by
Statement Reference Scer\GAL4^32B, rl^{Sem.C.Scer\UAS} has wing vein \| ectopic phenotype, suppressible by kay² (Gritzan et al., 2002) Scer\GAL4^32B, rl^{Sem.C.Scer\UAS} has wing vein \| ectopic phenotype, suppressible by Scer\GAL4^32B/kay^Fbz.Scer\UAS (Ciapponi et al., 2001)
NOT suppressed by
Statement Reference Scer\GAL4^32B, rl^{Sem.C.Scer\UAS} has wing vein \| ectopic phenotype, non-suppressible by kni² (Gritzan et al., 2002) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, rl^{Sem.C.Scer\UAS} has eye phenotype, non-suppressible by hppy^RB.Scer\UAS/Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Corl et al., 2009)
Enhancer of
Statement Reference Scer\GAL4^dpp.PH/rl^{Sem.C.Scer\UAS} is an enhancer of wing phenotype of Scer\GAL4^dpp.PH, eya^Scer\UAS.cHa (Hsiao et al., 2001)
Other
Statement Reference Scer\GAL4^32B, eg^18B, rl^{Sem.C.Scer\UAS} has wing phenotype (Gritzan et al., 2002, Gritzan et al., 2002)
Additional Comments
Genetic Interactions
Statement Reference Flies over-expressing rl[Sem.C.Scer\UAS] in neurons with Scer\GAL4[elav-C155] in a aru[8.128] background shows increased sensitivity to ethanol sedation that is not significantly different from that of aru[8.128] flies, indicating that a aru[8.128] background suppresses the decreased ethanol sensitivity caused by overexpression of rl[Sem.C.Scer\UAS]. (Eddison et al., 2011) Co-expression of hppy[RB.Scer\UAS] has no effect on the rough eye phenotype caused by expression of rl[Sem.C.Scer\UAS] under the control of Scer\GAL4[GMR.PF]. (Corl et al., 2009) The wing blister phenotype of flies expressing rl^{Sem.C.Scer\UAS} under the control of Scer\GAL4^Bx-MS1096 is not significantly enhanced by by²/+. (Lee et al., 2003) eg^18B flies expressing rl^{Sem.C.Scer\UAS} under the control of Scer\GAL4^32B have a held out wing phenotype. (Gritzan et al., 2002) Ectopic eyes in eya^Scer\UAS.cHa; Scer\GAL4^dpp.blk1; rl^{Sem.C.Scer\UAS} are generally larger, and have more ommatidial definition than those in eya^Scer\UAS.cHa; Scer\GAL4^dpp.blk1 flies. The proportion of eya^Scer\UAS.cHa; Scer\GAL4^dpp.PH flies with blistered wings (76%) is increased by heterozygosity for rl^{Sem.C.Scer\UAS} to 93%, while the proprotion with only mild wing defects is decreased from 24% to 7%. (All data from experiments with a single, 'strong' P{UAS-eya.H} insertion; n=approximately 300 in each case). (Hsiao et al., 2001)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Erk^act (Lievens et al., 2005) rl^{Sem.C.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 11 )
Research paper	Yue et al., 2012, Dev. Cell 22(2): 255--267 The cell adhesion molecule echinoid functions as a tumor suppressor and upstream regulator of the hippo signaling pathway. [FBrf0217474] Eddison et al., 2011, Neuron 70(5): 979--990 arouser Reveals a Role for Synapse Number in the Regulation of Ethanol Sensitivity. [FBrf0213908] Roignant and Treisman, 2010, Cell 143(2): 238--250 Exon Junction Complex Subunits Are Required to Splice Drosophila MAP Kinase, a Large Heterochromatic Gene. [FBrf0212083] Corl et al., 2009, Cell 137(5): 949--960 Happyhour, a Ste20 family kinase, implicates EGFR signaling in ethanol-induced behaviors. [FBrf0208186] Lee et al., 2008, Nat. Cell Biol. 10(4): 468--475 Drosophila short neuropeptide F signalling regulates growth by ERK-mediated insulin signalling. [FBrf0204120] Lievens et al., 2005, Hum. Mol. Genet. 14(5): 713--724 Expanded polyglutamine peptides disrupt EGF receptor signaling and glutamate transporter expression in Drosophila. [FBrf0184022] Hipfner and Cohen, 2003, PLoS Biol. 1(2): e35 The Drosophila Sterile-20 Kinase Slik Controls Cell Proliferation and Apoptosis during Imaginal Disc Development. [FBrf0167985] Lee et al., 2003, Development 130(17): 4001--4010 blistery encodes Drosophila tensin protein and interacts with integrin and the JNK signaling pathway during wing development. [FBrf0160724] Gritzan et al., 2002, Mech. Dev. 115(1-2): 91--100 Transrepression of AP-1 by nuclear receptors in Drosophila. [FBrf0149096] Ciapponi et al., 2001, Genes Dev. 15(12): 1540--1553 Drosophila Fos mediates ERK and JNK signals via distinct phosphorylation sites. [FBrf0136943] Hsiao et al., 2001, Dev. Cell 1(1): 51--61 Eyes absent mediates cross-talk between retinal determination genes and the receptor tyrosine kinase signaling pathway. [FBrf0141456]

Allele Dmel\rlSem.C.Scer\UAS

Allele Dmel\rl^{Sem.C.Scer\UAS}