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General Information
Symbol
Dmel\RafAct.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0124968
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Rafgof, RafAct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of a gain-of-function form of Raf.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Hearts from flies expressing Scer\GAL4tin.CΔ4>phlAct.Scer\UAS show reduced end-diastolic dimensions relative to controls. The transgenic hearts display myofiber disarray and increased heart wall thickness compared with controls. The number of cardiomyocytes is similar in the hearts from the transgenic flies to that of wild-type. Compared with controls, the transgenic hearts show an increase in cardiomyocyte ploidy, suggesting abnormal endoreplication.

Expression of phlAct.Scer\UAS in the midgut, under the control of Scer\GAL4esg-NP7397 results in the generation of many new midgut cells, including enterocyte-like cells.

Expression of phlAct.Scer\UAS under the control of Scer\GAL4CY2 results in a strong ectopic wing vein phenotype.

Expression of phlAct.Scer\UAS under the control of Scer\GAL4ey.PB can result in homeotic transformation of the eye to antenna. The ectopic antenna has all segments including the arista and is oriented similarly to the endogenous antenna.

Expression of phlAct.Scer\UAS under the control of Scer\GAL4dpp.blk1 results in ectopic furrow formation in the eye disc.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Phenotype Manifest In
Suppressed by
NOT suppressed by
Additional Comments
Genetic Interactions
Statement
Reference

The cardiac specific expression of rapGD9960 under the control of Scer\GAL4tin.CΔ4 prevents the phlAct.Scer\UAS-induced increases in cardiomyocyte ploidy.

Furrow initiation is blocked in eye discs coexpressing phlAct.Scer\UAS and NDN.Scer\UAS under the control of Scer\GAL4dpp.blk1.

Xenogenetic Interactions
Statement
Reference

Expression of Zzzz\lefScer\UAS.cGa suppresses the strong ectopic vein phenotype of phlAct.Scer\UAS under the control of Scer\GAL4CY2.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
RafAct.Scer\UAS
RafAct.UAS
phlAct.Scer\UAS
Name Synonyms
Secondary FlyBase IDs
    References (9)