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General Information
Symbol
Dmel\brnl.6P6
Species
D. melanogaster
Name
FlyBase ID
FBal0125109
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
brn1.6P6
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference
    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    brnfs.107/brnl.6P6 larvae have significantly increased bouton number and satellite bouton number at the neuromuscular junction of muscle 4, compared to controls, with no effect on bouton size.

    brnl.6P6 mutant larval peripheral nerves have a diameter comparable to wild-type nerves, and similarly to wild-type, do not show attached plasmatocytes.

    In brnl.6P6 third instar visual systems, R-cell axonal projections are defective and the lamina plexus is discontinuous and of variable thickness.

    Strong brn allele.

    Follicle cells line the wall of the germarium but only sometimes extend processes, leading to split cysts. They frequently lose their cuboidal or columnar shape and exit the epithelium around the oocyte and its adjacent nurse cells. Also follicle cells at the posterior end of the egg chamber become mesenchymal-like indicating they have not adopted a border cell fate.

    Homozygous females lay eggs with fused dorsal appendages: embryos show neurogenic phenotypes. Neurogenic phenotype of brnl.6P6 is more extreme than that of brnfs.107.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Phenotype Manifest In
    Enhanced by
    Statement
    Reference

    brnfs.107/brnl.6P6 has follicle cell phenotype, enhanceable by da[+]/da2

    NOT Enhanced by
    Statement
    Reference

    brnl.6P6 has photoreceptor cell R2 & axon phenotype, non-enhanceable by egh9pp4

    brnl.6P6 has photoreceptor cell R3 & axon phenotype, non-enhanceable by egh9pp4

    brnl.6P6 has photoreceptor cell R4 & axon phenotype, non-enhanceable by egh9pp4

    brnl.6P6 has photoreceptor cell R5 & axon phenotype, non-enhanceable by egh9pp4

    brnl.6P6 has photoreceptor cell R6 & axon phenotype, non-enhanceable by egh9pp4

    brnl.6P6 has photoreceptor cell R1 & axon phenotype, non-enhanceable by egh9pp4

    NOT suppressed by
    Statement
    Reference

    brnl.6P6, egh9pp4 has photoreceptor cell R4 & axon phenotype, non-suppressible by Hsap\B4GALT6UAS.cWa/Scer\GAL4arm.PS

    brnl.6P6, egh9pp4 has photoreceptor cell R5 & axon phenotype, non-suppressible by Hsap\B4GALT6UAS.cWa/Scer\GAL4arm.PS

    brnl.6P6, egh9pp4 has photoreceptor cell R6 & axon phenotype, non-suppressible by Hsap\B4GALT6UAS.cWa/Scer\GAL4arm.PS

    brnl.6P6, egh9pp4 has photoreceptor cell R1 & axon phenotype, non-suppressible by Hsap\B4GALT6UAS.cWa/Scer\GAL4arm.PS

    brnl.6P6, egh9pp4 has photoreceptor cell R2 & axon phenotype, non-suppressible by Hsap\B4GALT6UAS.cWa/Scer\GAL4arm.PS

    brnl.6P6, egh9pp4 has photoreceptor cell R3 & axon phenotype, non-suppressible by Hsap\B4GALT6UAS.cWa/Scer\GAL4arm.PS

    NOT Enhancer of
    Statement
    Reference

    brnl.6P6 is a non-enhancer of photoreceptor cell R4 & axon phenotype of egh9pp4

    brnl.6P6 is a non-enhancer of photoreceptor cell R5 & axon phenotype of egh9pp4

    brnl.6P6 is a non-enhancer of photoreceptor cell R6 & axon phenotype of egh9pp4

    brnl.6P6 is a non-enhancer of photoreceptor cell R1 & axon phenotype of egh9pp4

    brnl.6P6 is a non-enhancer of photoreceptor cell R2 & axon phenotype of egh9pp4

    brnl.6P6 is a non-enhancer of photoreceptor cell R3 & axon phenotype of egh9pp4

    Other
    Statement
    Reference
    Additional Comments
    Genetic Interactions
    Statement
    Reference

    The R-cell axonal projection phenotype of brnl.6P6 and egh9pp4 mutants is not enhanced when flies carry both mutations simultaneously.

    Eggs derived from brnl.6P6/brn+; grk3/grk+ females show an intermediate to strong fused dorsal appendage phenotype that is indistinguishable from eggs laid by brn females.

    Xenogenetic Interactions
    Statement
    Reference

    Expression of Hsap\β4Gal-T6Scer\UAS.cWa, under the control of Scer\GAL4arm.PS, in egh9pp4 mutants rescues the R-cell axonal projection phenotype. However, Scer\GAL4arm.PS > Hsap\β4Gal-T6Scer\UAS.cWa expression does not rescue the R-cell axonal projection phenotype in brnl.6P6 egh9pp4 double mutants.

    Complementation and Rescue Data
    Images (0)
    Mutant
    Wild-type
    Stocks (1)
    Notes on Origin
    Discoverer
    Comments
    Comments

    Allelic series in the ovary: brn228 > brnl.6P6 (germline clones) = brn198 > brnfs.107. Zygotic requirement for brn alleles (strongest to weakest): brnl.6P6 > brn228 > brn198 > brnfs.107.

    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (6)
    References (15)