Overexpression of Scer\UBP2Scer\UAS.cDa in the C4da neurons, under the control of Scer\GAL4ppk.PG results in some of the C4da neurons aberrantly retaining their larval dendritic arbors, through the prevention of efficient severing of dendrites from the soma.
Mushroom body γ neurons expressing Scer\UBP2Scer\UAS.cDa under the control of Scer\GAL4Tab2-201Y show major ultrastructural differences to wild-type neurons during developmental axon pruning; there is a decrease in the number of vacuoles and a general decrease in the interruption of axons compared to wild type at 12 hours after puparium formation (APF). The mutant axons maintain a readily identifiable microtubule cytoskeleton even at 12 hours APF (the microtubule cytoskeleton is absent or disrupted in wild-type animals at 6-12 hours APF). In addition, in the mutant neurons, many long axon profiles with wider diameters persist at 12 hours APF (in wild-type animals the length and diameter of axons appear to decrease during pruning). The frequency of multilamellar bodies is greatly reduced in the mutant neurons. The invasion of glical cells into the mushroom body lobes that is seen during developmental axon pruning in early pupae is unaffected in animals expressing Scer\UBP2Scer\UAS.cDa under the control of Scer\GAL4Tab2-201Y.
When Scer\UBP2Scer\UAS.cDa is driven by Scer\GAL4Tab2-201Y in the Mushroom Body γ neurons, during late larval stages, mutant neurons exhibit largely normal growth and guidance of axons. However at 18hr APF, the peak of axon pruning, mutant neurons fail to prune their axons. As a consequence γ neurons retain their dorsal lobes in the adult. When Scer\UBP2Scer\UAS.cDa is driven in the brain using either Scer\GAL4Tab2-201Y or Scer\GAL4OK107 as a driver, no effect is seen on cell proliferation.