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General Information
Symbol
Scer\UBP2UAS.cDa
Species
S. cerevisiae
Name
Saccharomyces cerevisiae UAS construct a of DiAntonio
FlyBase ID
FBal0125281
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-UBP2
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt sequences drive expression of Scer\UBP2.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Embryos derived from females expressing Scer\UBP2Scer\UAS.cDa under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 show a moderate but significant delay in the clearance of the paternal mitochondrial derivatives compared to wild type.

Expression of Scer\UBP2Scer\UAS.cDa in ddaC neurons under the control of Scer\GAL4ppk.1.9 results in dendrites that remain for many days after severing. Approximately 83% of animals still exhibit distal dendrites 18 hours after severing.

Pruning of ddaC dendrites is delayed upon expression of Scer\UBP2Scer\UAS.cDa under the control of Scer\GAL4ppk.1.9.

Overexpression of Scer\UBP2Scer\UAS.cDa in presynaptic neurons by Scer\GAL4OK6 does not affect synapse elimination.

Overexpression of Scer\UBP2Scer\UAS.cDa in postsynaptic muscles by Scer\GAL4C57 results in normal synaptic growth but delayed synaptic elimination.

Overexpression of Scer\UBP2Scer\UAS.cDa in the C4da neurons, under the control of Scer\GAL4ppk.PG results in some of the C4da neurons aberrantly retaining their larval dendritic arbors, through the prevention of efficient severing of dendrites from the soma.

Expression of Scer\UBP2Scer\UAS.cDa under the control of Scer\GAL4elav.PLu doubles the number of boutons per muscle surface area at the neuromuscular junction compared to wild type.

Mushroom body γ neurons expressing Scer\UBP2Scer\UAS.cDa under the control of Scer\GAL4Tab2-201Y show major ultrastructural differences to wild-type neurons during developmental axon pruning; there is a decrease in the number of vacuoles and a general decrease in the interruption of axons compared to wild type at 12 hours after puparium formation (APF). The mutant axons maintain a readily identifiable microtubule cytoskeleton even at 12 hours APF (the microtubule cytoskeleton is absent or disrupted in wild-type animals at 6-12 hours APF). In addition, in the mutant neurons, many long axon profiles with wider diameters persist at 12 hours APF (in wild-type animals the length and diameter of axons appear to decrease during pruning). The frequency of multilamellar bodies is greatly reduced in the mutant neurons. The invasion of glical cells into the mushroom body lobes that is seen during developmental axon pruning in early pupae is unaffected in animals expressing Scer\UBP2Scer\UAS.cDa under the control of Scer\GAL4Tab2-201Y.

When Scer\UBP2Scer\UAS.cDa is driven by Scer\GAL4Tab2-201Y in the Mushroom Body γ neurons, during late larval stages, mutant neurons exhibit largely normal growth and guidance of axons. However at 18hr APF, the peak of axon pruning, mutant neurons fail to prune their axons. As a consequence γ neurons retain their dorsal lobes in the adult. When Scer\UBP2Scer\UAS.cDa is driven in the brain using either Scer\GAL4Tab2-201Y or Scer\GAL4OK107 as a driver, no effect is seen on cell proliferation.

Over expression of Scer\UBP2Scer\UAS.cDa in the nervous system (driven by Scer\GAL4elav-C155) leads to a marked synaptic overgrowth and a severe reduction in presynaptic transmitter release.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Suppressor of
Phenotype Manifest In
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

Expression of Scer\UBP2Scer\UAS.cDa under the control of Scer\GAL4ey-OK107 fails to suppress the axon degeneration seen in bskflp147E mutant mushroom body neuron clones.

The increase in bouton number at the neuromuscular junction seen in larvae expressing Scer\UBP2Scer\UAS.cDa under the control of Scer\GAL4elav.PLu in a wild-type background is not seen if they express Scer\UBP2Scer\UAS.cDa under the control of Scer\GAL4elav.PLu in a witunspecified or Medunspecified background.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Scer\UBP2Scer\UAS.cDa
Scer\UBP2UAS.cDa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of DiAntonio
Secondary FlyBase IDs
    References (17)