FlyBase Allele Report: Dmel\Alk[UAS.cLa]

General Information

Symbol

Dmel\Alk^UAS.cLa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Loren

FlyBase ID

FBal0125507

Feature type

allele

Associated gene

Dmel\Alk

Associated Insertion(s)

Carried in Construct

P{UAS-Alk.L}

Also Known As

UAS-Alk, UAS-Alk^fl, UAS-Alk^WT

Key Links

Transgenic product class

cDNA

(Loren et al., 2001)

wild_type

(Loren et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Loren et al., 2001)

wild_type

(Loren et al., 2001)

Mutagen

in vitro construct

(Loren et al., 2001)

Progenitor genotype

Carried in construct

P{UAS-Alk.L}

Cytology

Description

UASt regulatory sequences drive expression of an Alk cDNA.

(Loren et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Alk

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cancer

CEA

(Wolfstetter et al., 2020)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of cancer

is ameliorated by BacA\p35^GMR.PH

(Wolfstetter et al., 2020)

is ameliorated by C3G^MS

(Wolfstetter et al., 2020)

is ameliorated by Diap1^GMR.PH

(Wolfstetter et al., 2020)

is ameliorated by Ras85D^e1B

(Wolfstetter et al., 2020)

is ameliorated by Sos^34Ea-7

(Wolfstetter et al., 2020)

is ameliorated by 14-3-3ε^S-696

(Wolfstetter et al., 2020)

is ameliorated by Tak1^UAS.cUa

(Wolfstetter et al., 2020)

is ameliorated by ave^CC9-36B

(Wolfstetter et al., 2020)

is ameliorated by hep^CA.UAS

(Wolfstetter et al., 2020)

is ameliorated by jeb^TM.3xOLLAS

(Wolfstetter et al., 2020)

is ameliorated by ksr^S-627

(Wolfstetter et al., 2020)

is ameliorated by pnt^Δ88

(Wolfstetter et al., 2020)

is ameliorated by puc^UAS.cMa

(Wolfstetter et al., 2020)

is ameliorated by tll^g

(Wolfstetter et al., 2020)

is ameliorated by wnd^UAS.cCa

(Wolfstetter et al., 2020)

is ameliorated by wnd^d00622

(Wolfstetter et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal learning | adult stage, with Scer\GAL4^Alk.38, Scer\GAL80^ts.αTub84B

(Gouzi et al., 2011)

abnormal learning | adult stage, with Scer\GAL4^elav-C155, Scer\GAL80^ts.αTub84B

(Gouzi et al., 2011)

abnormal learning | adult stage, with Scer\GAL4^Ras64B.PW, Scer\GAL80^ts.αTub84B

(Gouzi et al., 2011)

abnormal size | pupal stage, with Scer\GAL4^386Y

(Gouzi et al., 2011)

abnormal size | pupal stage, with Scer\GAL4^elav-C155

(Gouzi et al., 2011)

abnormal size | pupal stage, with Scer\GAL4^Ras64B.PW

(Gouzi et al., 2011)

decreased cell size | adult stage, with Scer\GAL4^Ras64B.PW

(Gouzi et al., 2011)

increased cell number | larval stage, with Scer\GAL4^He.PZ

(Zettervall et al., 2004)

lethal | embryonic stage, with Scer\GAL4^Act5C.PI

(Loren et al., 2001)

lethal | somatic clone, with Scer\GAL4^Act5C.PI

(Loren et al., 2001)

lethal - all die before end of larval stage, with Scer\GAL4^Alk.38

(Gouzi et al., 2011)

short lived, with Scer\GAL4^elav.PU

(Park et al., 2021)

some die during larval stage, with Scer\GAL4^Alk.38

(Gouzi et al., 2011)

viable, with Scer\GAL4^elav-C155

(Gouzi et al., 2011)

visible | adult stage, with Scer\GAL4^sev.PU

(Wolfstetter et al., 2017)

Phenotype Manifest In

embryonic somatic muscle cell, with Scer\GAL4^twi.PG

(Stute et al., 2004)

embryonic visceral muscle cell, with Scer\GAL4^twi.PG

(Stute et al., 2004)

eye, with Scer\GAL4^sev.EP

(Hugosson et al., 2014)

eye, with Scer\GAL4^sev.PU

(Wolfstetter et al., 2017)

hemocyte, with Scer\GAL4^He.PZ

(Zettervall et al., 2004)

lamellocyte, with Scer\GAL4^He.PZ

(Zettervall et al., 2004)

muscle founder cell & visceral mesoderm, with Scer\GAL4^twi.PG

(Stute et al., 2004)

ommatidium | adult stage, with Scer\GAL4^sev.PU

(Wolfstetter et al., 2017)

wing, with Scer\GAL4^Ras64B.PW

(Gouzi et al., 2011)

wing disc | somatic clone, with Scer\GAL4^Act5C.PI

(Loren et al., 2001)

Detailed Description

Statement

Reference

Expression of Alk^Scer\UAS.cLa under the control of Scer\GAL4^sev.PU leads to severe defects in ommatidia formation in the anterior part of the adult eye.

(Wolfstetter et al., 2017)

Scer\GAL4^sev.EP-mediated expression results in loss of ommatidia in the anterior region of the eye

(Hugosson et al., 2014)

Pan-neuronal expression of Alk^Scer\UAS.cLa under the control of Scer\GAL4^elav-C155, and restricted to the adult stages using Scer\GAL80^ts.αTub84B, causes significant deficits in associative olfactory learning compared to controls.

Expression of Alk^Scer\UAS.cLa under the control of Scer\GAL4^Ras64B.PW, and restricted to the adult stages using Scer\GAL80^ts.αTub84B, causes significant deficits in associative olfactory learning compared to controls. The wings of the resulting adult flies are also smaller than in wild type, due to reduced cell size rather than cell number.

Expression of Alk^Scer\UAS.cLa under the control of Scer\GAL4^Alk.38, and restricted to the adult stages using Scer\GAL80^ts.αTub84B, causes significant deficits in associative olfactory learning compared to controls.

Expression of Alk^Scer\UAS.cLa under the control of either Scer\GAL4^Mef2.247 or Scer\GAL4^c747, and restricted to the adult stages using Scer\GAL80^ts.αTub84B, does not affect associative olfactory learning.

Pan-neuronal expression of Alk^Scer\UAS.cLa under the control of Scer\GAL4^elav-C155 throughout development yields pupae that are 10-15% smaller than controls. The resulting adults are viable and normally patterned.

Expression of Alk^Scer\UAS.cLa under the control of Scer\GAL4^Ras64B.PW or Scer\GAL4^386Y throughout development yields pupae that are 10-15% smaller than controls.

Expression of Alk^Scer\UAS.cLa under the control of Scer\GAL4^Alk.38 throughout development results in larval lethality.

Expression of Alk^Scer\UAS.cLa under the control of Scer\GAL4^Ilp2.PR throughout development yields pupae that are a similar size to controls.

(Gouzi et al., 2011)

In early stage 11 Alk^Scer\UAS.cLa; Scer\GAL4^twi.PG embryos, the visceral mesoderm contains fusion competent myoblasts, but no muscle founder cells. At later stages, visceral mesoderm derivatives appear to be absent from these embryos, and the somatic musculature is defective: Several muscles are small and display a spindle-like shape with long and thin projections, indicating that only few myoblasts fuse to form the muscles. In Alk^Scer\UAS.cLa; Scer\GAL4^bap.3 embryos the muscle founder cells of the visceral mesoderm are present and may be increased in number.

(Stute et al., 2004)

When expression is driven by Scer\GAL4^He.PZ, hemocyte proliferation is increased and lamellocyte numbers are increased though crystal cells are unaffected.

(Zettervall et al., 2004)

Large clones in the wing disc expressing Alk^Scer\UAS.cLa under the control of Scer\GAL4^Act5C.PI result in disruption of disc development. Animals carrying clones expressing Alk^Scer\UAS.cLa under the control of Scer\GAL4^Act5C.PI do not survive to adulthood.

(Loren et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Alk^UAS.cLa, Scer\GAL4^elav.PU has short lived phenotype, enhanceable by Hsap\MAPT^UAS.cWa, Scer\GAL4^elav.PU

(Park et al., 2021)

Suppressed by

Statement

Reference

Alk^UAS.cLa, Scer\GAL4^sev.PU has visible | adult stage phenotype, suppressible | partially by Scer\GAL4^sev.PU/cnk^{5xAIR.UAS.Tag:HA}

(Wolfstetter et al., 2017)

Alk^UAS.cLa, Scer\GAL4^sev.PU has visible | adult stage phenotype, suppressible by Scer\GAL4^sev.PU/cnk^{5xAIR.UAS.Tag:Myr(Src42A).Tag:HA}

(Wolfstetter et al., 2017)

Enhancer of

Statement

Reference

Alk^UAS.cLa, Scer\GAL4^elav.PU is an enhancer of short lived phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^elav.PU

(Park et al., 2021)

Other

Statement

Reference

Alk^UAS.cLa, Nf1^E2, Scer\GAL4^elav-C155 has lethal | recessive | larval stage phenotype

(Gouzi et al., 2011)

Alk^UAS.cLa, Nf1^E2, Scer\GAL4^elav-C155 has lethal | dominant | larval stage phenotype

(Gouzi et al., 2011)

Alk^UAS.cLa, Nf1^E2, Scer\GAL4^Ras64B.PW has lethal | recessive | larval stage phenotype

(Gouzi et al., 2011)

Alk^UAS.cLa, Nf1^E2, Scer\GAL4^386Y has lethal | recessive | larval stage phenotype

(Gouzi et al., 2011)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Alk^UAS.cLa, Scer\GAL4^sev.EP has eye phenotype, non-enhanceable by miple1^UAS.cHa, Scer\GAL4^sev.EP

(Hugosson et al., 2014)

Alk^UAS.cLa, Scer\GAL4^sev.EP has eye phenotype, non-enhanceable by miple2^UAS.Venus, Scer\GAL4^sev.EP

(Hugosson et al., 2014)

Alk^UAS.cLa, Scer\GAL4^sev.EP has eye phenotype, non-enhanceable by Df(3L)miple1-2^Δ104

(Hugosson et al., 2014)

Suppressed by

Statement

Reference

Alk^UAS.cLa, Scer\GAL4^sev.PU has eye phenotype, suppressible | partially by Scer\GAL4^sev.PU/cnk^{5xAIR.UAS.Tag:HA}

(Wolfstetter et al., 2017)

Alk^UAS.cLa, Scer\GAL4^sev.PU has ommatidium | adult stage phenotype, suppressible | partially by Scer\GAL4^sev.PU/cnk^{5xAIR.UAS.Tag:HA}

(Wolfstetter et al., 2017)

Alk^UAS.cLa, Scer\GAL4^sev.PU has eye phenotype, suppressible by Scer\GAL4^sev.PU/cnk^{5xAIR.UAS.Tag:Myr(Src42A).Tag:HA}

(Wolfstetter et al., 2017)

Alk^UAS.cLa, Scer\GAL4^sev.PU has ommatidium | adult stage phenotype, suppressible by Scer\GAL4^sev.PU/cnk^{5xAIR.UAS.Tag:Myr(Src42A).Tag:HA}

(Wolfstetter et al., 2017)

NOT suppressed by

Statement

Reference

Alk^UAS.cLa, Scer\GAL4^sev.EP has eye phenotype, non-suppressible by miple1^UAS.cHa, Scer\GAL4^sev.EP

(Hugosson et al., 2014)

Alk^UAS.cLa, Scer\GAL4^sev.EP has eye phenotype, non-suppressible by miple2^UAS.Venus, Scer\GAL4^sev.EP

(Hugosson et al., 2014)

Alk^UAS.cLa, Scer\GAL4^sev.EP has eye phenotype, non-suppressible by Df(3L)miple1-2^Δ104

(Hugosson et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

The disrupted morphology of ommatidia in the anterior part of the adult eye of flies expressing Alk^Scer\UAS.cLa under the control of Scer\GAL4^sev.PU is partially suppressed by co-expression of cnk^{5xAIR.Scer\UAS.T:Ivir\HA1} and even more strongly by co-expression of cnk^{5xAIR.Scer\UAS.T:Myr-Src42A.T:Ivir\HA1}.

(Wolfstetter et al., 2017)

Expression of Alk^Scer\UAS.cLa under the control of Scer\GAL4^elav-C155, Scer\GAL4^Ras64B.PW or Scer\GAL4^386Y in a Nf1^E2 mutant background results in larval lethality. Larval lethality is also seen when Alk^Scer\UAS.cLa is expressed under the control of Scer\GAL4^elav-C155 in a Nf1^E2/+ background.

(Gouzi et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Alk^UAS.cLa/Scer\GAL4^386Y rescues Alk^ts

(Bai and Sehgal, 2015)

Alk^UAS.cLa/Scer\GAL4¹⁴⁷¹ rescues Alk^ts

(Bai and Sehgal, 2015)

Scer\GAL4^bap.3/Alk^UAS.cLa rescues Alk^mili

(Stute et al., 2004)

Partially rescues

Alk^UAS.cLa/Scer\GAL4^30Y partially rescues Alk^ts

(Bai and Sehgal, 2015)

Scer\GAL4^121Y/Alk^UAS.cLa partially rescues Alk^ts

(Bai and Sehgal, 2015)

Fails to rescue

Scer\GAL4^007Y/Alk^UAS.cLa fails to rescue Alk^ts

(Bai and Sehgal, 2015)

Scer\GAL4^MJ63/Alk^UAS.cLa fails to rescue Alk^ts

(Bai and Sehgal, 2015)

Comments

In combination with Scer\GAL80^ts.αTub84B at restrictive 29[o]C, expression of Alk^Scer\UAS.cLa driven by Scer\GAL4^386Y or Scer\GAL4¹⁴⁷¹ fully rescues the increase of total sleep seen in Alk^ts/Alk^ts females; expression driven by Scer\GAL4^30Y or Scer\GAL4^121Y leads to partial rescue and expression driven by Scer\GAL4^007Y or Scer\GAL4^MJ63 does not rescue the phenotype.

(Bai and Sehgal, 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Alk^Scer\UAS.cLa

Alk^UAS.cLa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Loren

(Loren et al., 2001)

Secondary FlyBase IDs

References (17)

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