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General Information
Symbol
Dmel\AlkUAS.cLa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Loren
FlyBase ID
FBal0125507
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Alk, UAS-AlkWT
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an Alk cDNA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of AlkScer\UAS.cLa under the control of Scer\GAL4sev.PU leads to severe defects in ommatidia formation in the anterior part of the adult eye.

Scer\GAL4sev.EP-mediated expression results in loss of ommatidia in the anterior region of the eye

Pan-neuronal expression of AlkScer\UAS.cLa under the control of Scer\GAL4elav-C155, and restricted to the adult stages using Scer\GAL80ts.αTub84B, causes significant deficits in associative olfactory learning compared to controls.

Expression of AlkScer\UAS.cLa under the control of Scer\GAL4Ras64B.PW, and restricted to the adult stages using Scer\GAL80ts.αTub84B, causes significant deficits in associative olfactory learning compared to controls. The wings of the resulting adult flies are also smaller than in wild type, due to reduced cell size rather than cell number.

Expression of AlkScer\UAS.cLa under the control of Scer\GAL4Alk.38, and restricted to the adult stages using Scer\GAL80ts.αTub84B, causes significant deficits in associative olfactory learning compared to controls.

Expression of AlkScer\UAS.cLa under the control of either Scer\GAL4Mef2.247 or Scer\GAL4c747, and restricted to the adult stages using Scer\GAL80ts.αTub84B, does not affect associative olfactory learning.

Pan-neuronal expression of AlkScer\UAS.cLa under the control of Scer\GAL4elav-C155 throughout development yields pupae that are 10-15% smaller than controls. The resulting adults are viable and normally patterned.

Expression of AlkScer\UAS.cLa under the control of Scer\GAL4Ras64B.PW or Scer\GAL4386Y throughout development yields pupae that are 10-15% smaller than controls.

Expression of AlkScer\UAS.cLa under the control of Scer\GAL4Alk.38 throughout development results in larval lethality.

Expression of AlkScer\UAS.cLa under the control of Scer\GAL4Ilp2.PR throughout development yields pupae that are a similar size to controls.

In early stage 11 AlkScer\UAS.cLa; Scer\GAL4twi.PG embryos, the visceral mesoderm contains fusion competent myoblasts, but no muscle founder cells. At later stages, visceral mesoderm derivatives appear to be absent from these embryos, and the somatic musculature is defective: Several muscles are small and display a spindle-like shape with long and thin projections, indicating that only few myoblasts fuse to form the muscles. In AlkScer\UAS.cLa; Scer\GAL4bap.3 embryos the muscle founder cells of the visceral mesoderm are present and may be increased in number.

When expression is driven by Scer\GAL4He.PZ, hemocyte proliferation is increased and lamellocyte numbers are increased though crystal cells are unaffected.

Large clones in the wing disc expressing AlkScer\UAS.cLa under the control of Scer\GAL4Act5C.PI result in disruption of disc development. Animals carrying clones expressing AlkScer\UAS.cLa under the control of Scer\GAL4Act5C.PI do not survive to adulthood.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
NOT Enhanced by
Suppressed by
NOT suppressed by
Additional Comments
Genetic Interactions
Statement
Reference

The disrupted morphology of ommatidia in the anterior part of the adult eye of flies expressing AlkScer\UAS.cLa under the control of Scer\GAL4sev.PU is partially suppressed by co-expression of cnk5xAIR.Scer\UAS.T:Ivir\HA1 and even more strongly by co-expression of cnk5xAIR.Scer\UAS.T:Myr-Src42A.T:Ivir\HA1.

Expression of AlkScer\UAS.cLa under the control of Scer\GAL4elav-C155, Scer\GAL4Ras64B.PW or Scer\GAL4386Y in a Nf1E2 mutant background results in larval lethality. Larval lethality is also seen when AlkScer\UAS.cLa is expressed under the control of Scer\GAL4elav-C155 in a Nf1E2/+ background.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

In combination with Scer\GAL80ts.αTub84B at restrictive 29[o]C, expression of AlkScer\UAS.cLa driven by Scer\GAL4386Y or Scer\GAL41471 fully rescues the increase of total sleep seen in Alkts/Alkts females; expression driven by Scer\GAL430Y or Scer\GAL4121Y leads to partial rescue and expression driven by Scer\GAL4007Y or Scer\GAL4MJ63 does not rescue the phenotype.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
AlkScer\UAS.cLa
AlkUAS.cLa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Loren
Secondary FlyBase IDs
    References (14)