FlyBase Allele Report: Dmel\dome[G0468]

General Information

Symbol

Dmel\dome^G0468

Species

D. melanogaster

Name

FlyBase ID

FBal0126401

Feature type

allele

Associated gene

Dmel\dome

Associated Insertion(s)

P{lacW}dome^G0468

Carried in Construct

Also Known As

dome⁴⁶⁸

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Castelli-Gair Hombria, 2001.9.19)

Mutagen

P-element activity

(Peter et al., 2002, Brown et al., 2001, Castelli-Gair Hombria, 2001.9.19)

Progenitor genotype

Associated Insertion(s)

P{lacW}dome^G0468

Cytology

Description

The P{lacW} insertion is at nucleotide +268 of the 4.8kb dome transcript.

(Josten et al., 2004)

P{lacW} insertion in the 5' UTR, at nucleotide position 268 of dome EST LD32858.

(Brown et al., 2001)

Insertion of P{lacW} into 5' UTR of dome, at position of 268bp with respect to the start of LD32858.

(Castelli-Gair Hombria, 2001.9.19)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

X:19,682,958..19,683,300 [+]

(Josten et al., 2004, Peter et al., 2002, Brown et al., 2001, Castelli-Gair Hombria, 2001.9.19)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

AJ427008

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Castelli-Gair Hombria, 2001.9.19)

lethal - all die before end of larval stage

(Josten et al., 2004, Peter et al., 2002)

some die during larval stage

(Josten et al., 2004, Peter et al., 2002)

Phenotype Manifest In

embryonic/larval posterior spiracle

(Brown et al., 2001)

embryonic/larval proventriculus

(Josten et al., 2004)

embryonic/larval spiracle

(Lovegrove et al., 2006, Castelli-Gair Hombria, 2001.9.19)

filzkorper

(Brown et al., 2001)

stigmatophore

(Brown et al., 2001)

Detailed Description

Statement

Reference

dome^G0468 mutants exhibit abnormal spiracles due to a lack of cell elongation.

(Lovegrove et al., 2006)

Mutant animals are lethal at the first instar larval stage and show a phenotype in the gastrointestinal tract indicate by a block of food passage at the defective proventriculus organ. A failure of ectodermal cells to invaginate and a disorganised endodermal layer of the proventriculus is seen.

(Josten et al., 2004)

Lethality occurs during the first larval instar.

(Peter et al., 2002)

The shape of the posterior spiracles is abnormal in mutants; the stigmatophore does not protrude and only the most external part of the filzkorper is formed.

(Brown et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Other

Statement

Reference

Df(2L)32FP-5, ct^db7, dome^G0468 has embryonic/larval spiracle phenotype

(Lovegrove et al., 2006)

Df(2L)32FP-5, ct^db7, dome^G0468, ems² has embryonic/larval spiracle phenotype

(Lovegrove et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

In ct^db7, dome^G0468, Df(2L)32FP-5 (deficiency for sal) triple mutants, no obvious spiracle structures remain, but the trachea still joins to the outside.

Quadruple ct^db7, Df(2L)32FP-5 (deficiency for sal), ems², dome^G0468 mutants are missing spiracle structures completely.

(Lovegrove et al., 2006)

The enlarged eye phenotype seen in flies carrying one copy of upd1^GMR.PB is moderately suppressed by dome^G0468.

(Bach et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

dome^G0217

(Josten et al., 2004)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Mobilisation of the P-element reverts both the lethality and posterior spiracle phenotype.

(Brown et al., 2001)

External Crossreferences and Linkouts ( 1 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

AJ427008

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

dome⁴⁶⁸

(Hatini et al., 2013, Sotillos et al., 2008, Arbouzova et al., 2006, Lovegrove et al., 2006, Hombria et al., 2005, Bach et al., 2003, Brown et al., 2001, Castelli-Gair Hombria, 2001.9.19)

dome^G0468

(Xu et al., 2011, Gutierrez-Aviño et al., 2009)

l(1)G0468

(Peter et al., 2002)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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