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General Information
Symbol
Dmel\domeΔCYT.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0126406
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-domeΔCYT, UAS-domeΔCYT, UAS-domeDN, DomeΔCYT, domeDN, UAS-dome.ΔCYT, UAS-domeΔCYT3.2, UAS-domeΔcyt2.1
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of a truncated form of dome which lacks the intracellular domain.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 1 )
 

In a tuberculosis model by abdominal injection with Mycobacterium marinum, adult flies expressing domeΔCYT.Scer\UAS under the control of Scer\GAL4crq.Unk, in combination with gal80[ts] to restrict expression to adulthood, present a significant decrease in bacterial burden, as compared to wild-type control flies.

Phenotypic Data
Phenotypic Class
Phenotype Manifest In

egg chamber & basement membrane | apical, with Scer\GAL4slbo.2.6

Detailed Description
Statement
Reference

The expression of domeΔCYT.Scer\UAS under the control of Scer\GAL4crq.Unk, in combination with gal80[ts] to restrict expression to adulthood, leads to a significant decrease in bacterial burden in adults infected with Mycobacterium marinum, as compared to wild-type controls.

The expression of domeΔCYT.Scer\UAS under the control of Scer\GAL4sd.PU leads to a severe reduction in the overall size of the third instar larval wing disc, which is particularly evident on the posterior compartment (even occasionally leading to a complete loos of the compartment), as compared to controls. Expression under the control of Scer\GAL4en-e16E or Scer\GAL4hh.PU also leads to a severe decrease in size, the former also being associated with a severe increase in apoptosis, in the third instar larval wing disc posterior compartment, as compared to controls. Expression under the control of Scer\GAL4ci.PU gives rise to a reduction in the presumptive hinge size, but does does not induce any ectopic wing structures, in third instar larval wing disc, as compared to controls. Expression under the control of Scer\GAL4hth.PU leads to an expanded third instar larval wing pouch and to pattern duplications in the posterior compartment of the adult wing, as compared to controls.

Expression of domeΔCYT.Scer\UAS using Scer\GAL4Act.PU increases mortality rates after infection with Drosophila C virus (DCV) compared with wild-type.

Expression of domeΔCYT.Scer\UAS under the control of Scer\GAL4byn-Gal4 results in stage 15 embryonic hindguts having a lower angle of curvature, as compared to controls.

Expression of domeΔCYT.Scer\UAS under the control of any of the following: Scer\GAL4esg.PU, Scer\GAL4Su(H).GBE or Scer\GAL4how-24B significantly reduces the increase in the rate of intestinal stem cells division normally observed in the adult midgut upon bacterial infection challenge. The increase in division rate is not diminished when domeΔCYT.Scer\UAS is expressed under the control of either Scer\GAL4Dl-05151-G or Scer\GAL4Myo31DF.PU.

Expression of domeΔCYT.Scer\UAS under the control of Scer\GAL4tim.PE does not disrupt locomotor activity rhythms.

Expression of domeΔCYT.Scer\UAS under the control of Scer\GAL4dome-PG14 results in defects in locomotor activity rhythms (only 15.4% of flies show rhythmic behaviour under constant darkness conditions).

Expression of domeΔCYT.Scer\UAS under the control of Scer\GAL4Mef2.247.Switch specifically during the adult stage (in the presence of RU486) causes a defect in long term memory, without affecting anesthesia-resistant memory or short term memory.

Expression of domeΔCYT.Scer\UAS under the control of Scer\GAL4Act.PU slightly decreases eye disc growth compared to wild type.

Expression of domeΔCYT.Scer\UAS under the control of Scer\GAL4ey.PU results in a strong reduction in the overall volume of the eye and a loss of ventral eye tissue.

Expression of domeΔCYT.Scer\UAS in border cells, driven by Scer\GAL4slbo.2.6, induces most egg chambers to develop without outer border cells. Consequently, the two polar cells remain at the anterior tip and do not migrate. The apical cap forms normally in Scer\GAL4slbo.2.6>domeΔCYT.Scer\UAS stage 9 egg chambers but is not shed in later stage egg chambers.

Expression of domeΔCYT.Scer\UAS, under the control of Scer\GAL4slbo.2.6, causes slow migration of border cells. These cells often migrate as single cells rather than clusters.

Expression of domeΔCYT.Scer\UAS under the control of Scer\GAL4ey.PH results in a small-eye phenotype.

Expression of domeΔCYT.Scer\UAS under the control of Scer\GAL4Eq1 results in a reduction in the number of mitotic cells in the third instar larval eye disc in the first mitotic wave.

Expression of domeΔCYT.Scer\UAS under the control of Scer\GAL4byn-Gal4 results in embryos with significantly shorter and wider hindguts than normal. There are a greater number of cells in the circumference of the mutant hindgut than in wild type.

Egg chambers expressing domeΔCYT.Scer\UAS under the control of Scer\GAL4slbo.2.6 lack outer border follicle cells.

Expression of domeΔCYT.Scer\UAS under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 results in segmentation defects in approximately 50% of embryos. The most frequent defects are deletions and fusions of segments A4 and A5.

When domeΔCYT.Scer\UAS is driven by Scer\GAL4slbo.2.6, dramatic recruitment and migration defects in the border cells are observed. The average number of outer border cells in these egg chambers is 0.5.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
NOT suppressed by
NOT Enhancer of
Statement
Reference
Suppressor of
NOT Suppressor of
Phenotype Manifest In
Enhanced by
Suppressed by
NOT suppressed by
Suppressor of
Statement
Reference

domeΔCYT.UAS/Scer\GAL4ey.PH is a suppressor of eye phenotype of Lfee

NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

The cell non-autonomous increase in autophagosomes (assessed by a Atg8a fluorescence reporter) in neighboring tissue resulting from the presence of clones homozygous for scrib673 and expressing Ras85DV12.Scer\UAS under the control of Scer\GAL4Act.PU in third instar larval eye-antennal imaginal discs is suppressed by the additional clonal co-expression of domeΔCYT.Scer\UAS.

The severe decrease in wing disc posterior compartment size induced by the expression of domeΔCYT.Scer\UAS under the control of Scer\GAL4en-e16E is partially suppressed by heterozygosity for Df(2R)enE, or by the co-expression of Diap1Scer\UAS.T:Hsap\MYC.cBa, DroncGD12376, CycAScer\UAS.cWa, enJF02316 or enKK107874, but not by the co-expression of CycBScer\UAS.T:Ivir\HA1; the associated severe increase in apoptosis in the posterior compartment is also suppressed by the co-expression of Diap1Scer\UAS.T:Hsap\MYC.cBa, DroncGD12376 or enKK107874.

The co-expression of domeΔCYT.Scer\UAS suppresses the tumor phenotype induced by the expression of scribHMS01490 under the control of Scer\GAL4Bx-MS1096.

Expression of domeΔCYT.Scer\UAS suppresses the overgrowth and invasion of the nerve cord seen in scrib1 mutant eye-antennal disc clones that simultaneously express Ras85DV12.Scer\UAS under the control of Scer\GAL4Act.PU.

Expression of domeΔCYT.Scer\UAS in eye-antennal disc clones that express Ras85DV12.Scer\UAS under the control of Scer\GAL4Act.PU, and in which adjacent cells are mutant for scrib1, suppresses the overgrowth and invasion of the nerve cord.

Expression of domeΔCYT.Scer\UAS in eye-antennal disc clones that express Ras85DV12.Scer\UAS and upd1Scer\UAS.cUa under the control of Scer\GAL4Act.PU suppresses the overgrowth and invasion of the nerve cord.

Expression of domeΔCYT.Scer\UAS under the control of Scer\GAL4Act.PU reduces the overgrowth of Sce1 and of Pc15 mutant eye discs.

Expression of domeΔCYT.Scer\UAS under the control of Scer\GAL4ey.PH in a Lfee mutant background restores eye size to normal.

The frequency of overgrowths observed in elB3.3.1 nocd64 double mutant ey clones is not affected when the JAK-STAT pathway is blocked by expression of dominant-negative domeΔCYT.Scer\UAS (under the control of Scer\GAL4Scer\FRT.Act5C).

Co-expression of domeΔCYT.Scer\UAS completely suppresses the extra border follicle cells produced by expression of upd1Scer\UAS.cZa under the control of Scer\GAL4slbo.2.6.

The penetrance and expressivity of the segmentation defects of embryos expressing domeΔCYT.Scer\UAS under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 is enhanced if the mothers are also heterozygous for hopunspecified.

Xenogenetic Interactions
Statement
Reference

The severe decrease in wing disc posterior compartment size induced by the expression of domeΔCYT.Scer\UAS under the control of Scer\GAL4en-e16E is partially suppressed by the co-expression of BacA\p35Scer\UAS.cHa.

Complementation and Rescue Data
Comments

The co-expression of domeΔCYT.Scer\UAS suppresses the non-autonomous induction of ectopic wing structures in third instar larval wing discs resulting from the expression of domeHMS01293 under the control of Scer\GAL4ci.PU.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Symbol Synonym
domeΔCYT.Scer\UAS
domeΔCYT.UAS
Name Synonyms
Secondary FlyBase IDs
    References (39)