Allele Dmel\dome^{ΔCYT.Scer\UAS}

General Information
Symbol	Dmel\dome^{ΔCYT.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0126406
Feature type	allele	Associated gene	Dmel\dome

Allele class
Mutagen	in vitro construct - deletion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - deletion (Castelli-Gair Hombria, 2001.9.19)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of a truncated form of dome which lacks the intracellular domain. (Brown et al., 2001)
Carried in construct	P{UAS-dome.ΔCYT} (Medioni and Noselli, 2005, Silver et al., 2005, Castelli-Gair Hombria, 2001.9.19, Dostert et al., 2005, Silver and Montell, 2001, Brown et al., 2001, Bach et al., 2003, Johansen et al., 2003, Ghiglione et al., 2002, Pinheiro and Montell, 2004, Agaisse et al., 2003, Arbouzova et al., 2006, Luque and Milan, 2007, Wang and Huang, 2009, Wang et al., 2010, Tsai and Sun, 2004, Karsten et al., 2006, Copf et al., 2011, Luo and Sehgal, 2012, Furriols et al., 2007)
Cytology
Phenotypic Data
Phenotypic Class
	locomotor rhythm defective, with Scer\GAL4^dome-PG14 (Luo and Sehgal, 2012) memory defective \| adult stage \| RU486 conditional, with Scer\GAL4^{Mef2.247.Switch} (Copf et al., 2011) visible, with Scer\GAL4^ey.PH (Tsai and Sun, 2004) visible, with Scer\GAL4^ey.PU (Karsten et al., 2006)
Phenotype Manifest In
	border follicle cell, with Scer\GAL4^slbo.2.6 (Medioni and Noselli, 2005, Ghiglione et al., 2002, Silver et al., 2005, Silver and Montell, 2001) egg chamber & basement membrane \| apical, with Scer\GAL4^slbo.2.6 (Medioni and Noselli, 2005) embryonic abdominal segment 4, with Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} (Brown et al., 2001) embryonic abdominal segment 5, with Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} (Brown et al., 2001) embryonic hindgut, with Scer\GAL4^byn-Gal4 (Johansen et al., 2003) embryonic segment, with Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} (Brown et al., 2001) eye, with Scer\GAL4^ey.PH (Tsai and Sun, 2004) eye, with Scer\GAL4^ey.PU (Karsten et al., 2006) eye disc \| somatic clone, with Scer\GAL4^Act.PU (Classen et al., 2009) eye disc anterior to the morphogenetic furrow, with Scer\GAL4^Eq1 (Tsai and Sun, 2004) polar follicle cell, with Scer\GAL4^slbo.2.6 (Medioni and Noselli, 2005)
Detailed Description
	Statement Reference Expression of dome[ΔCYT.Scer\UAS] under the control of Scer\GAL4[tim.PE] does not disrupt locomotor activity rhythms. Expression of dome[ΔCYT.Scer\UAS] under the control of Scer\GAL4[dome-PG14] results in defects in locomotor activity rhythms (only 15.4% of flies show rhythmic behaviour under constant darkness conditions). (Luo and Sehgal, 2012) Expression of dome[ΔCYT.Scer\UAS] under the control of Scer\GAL4[Mef2.247.Switch] specifically during the adult stage (in the presence of RU486) causes a defect in long term memory, without affecting anesthesia-resistant memory or short term memory. (Copf et al., 2011) Expression of dome[ΔCYT.Scer\UAS] under the control of Scer\GAL4[Act.PU] slightly decreases eye disc growth compared to wild type. (Classen et al., 2009) Expression of dome[ΔCYT.Scer\UAS] under the control of Scer\GAL4[ey.PU] results in a strong reduction in the overall volume of the eye and a loss of ventral eye tissue. (Karsten et al., 2006) Expression of dome^{ΔCYT.Scer\UAS} in border cells, driven by Scer\GAL4^slbo.2.6, induces most egg chambers to develop without outer border cells. Consequently, the two polar cells remain at the anterior tip and do not migrate. The apical cap forms normally in Scer\GAL4^slbo.2.6>dome^{ΔCYT.Scer\UAS} stage 9 egg chambers but is not shed in later stage egg chambers. (Medioni and Noselli, 2005) Expression of dome^{ΔCYT.Scer\UAS}, under the control of Scer\GAL4^slbo.2.6, causes slow migration of border cells. These cells often migrate as single cells rather than clusters. (Silver et al., 2005) Expression of dome[ΔCYT.Scer\UAS] under the control of Scer\GAL4[ey.PH] results in a small-eye phenotype. Expression of dome[ΔCYT.Scer\UAS] under the control of Scer\GAL4[Eq1] results in a reduction in the number of mitotic cells in the third instar larval eye disc in the first mitotic wave. (Tsai and Sun, 2004) Expression of dome^{ΔCYT.Scer\UAS} under the control of Scer\GAL4^byn-Gal4 results in embryos with significantly shorter and wider hindguts than normal. There are a greater number of cells in the circumference of the mutant hindgut than in wild type. (Johansen et al., 2003) Egg chambers expressing dome^{ΔCYT.Scer\UAS} under the control of Scer\GAL4^slbo.2.6 lack outer border follicle cells. (Ghiglione et al., 2002) Expression of dome^{ΔCYT.Scer\UAS} under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} results in segmentation defects in approximately 50% of embryos. The most frequent defects are deletions and fusions of segments A4 and A5. (Brown et al., 2001) When dome^{ΔCYT.Scer\UAS} is driven by Scer\GAL4^slbo.2.6, dramatic recruitment and migration defects in the border cells are observed. The average number of outer border cells in these egg chambers is 0.5. (Silver and Montell, 2001)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference dome^{ΔCYT.Scer\UAS}/Scer\GAL4^Act.PU is a suppressor \| somatic clone of tumorigenic \| somatic clone phenotype of Pc¹⁵ (Classen et al., 2009) dome^{ΔCYT.Scer\UAS}/Scer\GAL4^Act.PU is a suppressor \| somatic clone of tumorigenic \| somatic clone phenotype of Sce¹ (Classen et al., 2009) dome^{ΔCYT.Scer\UAS}/Scer\GAL4^ey.PH is a suppressor of size defective phenotype of L^fee (Wang and Huang, 2009)
NOT Suppressor of
Statement Reference dome^{ΔCYT.Scer\UAS}/Scer\GAL4^{Scer\FRT.Act5C} is a non-suppressor of increased cell number phenotype of elB^3.3.1, noc^d64 (Luque and Milan, 2007)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}, dome^{ΔCYT.Scer\UAS} has embryonic segment phenotype, enhanceable by hop^unspecified (Brown et al., 2001)
Suppressor of
Statement Reference dome^{ΔCYT.Scer\UAS}/Scer\GAL4^Act.PU is a suppressor \| somatic clone of eye disc \| somatic clone phenotype of Pc¹⁵ (Classen et al., 2009) dome^{ΔCYT.Scer\UAS}/Scer\GAL4^Act.PU is a suppressor \| somatic clone of eye disc \| somatic clone phenotype of Sce¹ (Classen et al., 2009) dome^{ΔCYT.Scer\UAS}/Scer\GAL4^ey.PH is a suppressor of eye phenotype of L^fee (Wang and Huang, 2009) dome^{ΔCYT.Scer\UAS}/Scer\GAL4^slbo.2.6 is a suppressor of border follicle cell \| supernumerary phenotype of Scer\GAL4^slbo.2.6, os^Scer\UAS.cZa (Ghiglione et al., 2002)
NOT Suppressor of
Statement Reference dome^{ΔCYT.Scer\UAS}/Scer\GAL4^{Scer\FRT.Act5C} is a non-suppressor of eye-antennal disc \| somatic clone phenotype of elB^3.3.1, noc^d64 (Luque and Milan, 2007) dome^{ΔCYT.Scer\UAS}/Scer\GAL4^{Scer\FRT.Act5C} is a non-suppressor of head capsule \| somatic clone phenotype of elB^3.3.1, noc^d64 (Luque and Milan, 2007)
Additional Comments
Genetic Interactions
Statement Reference Expression of dome[ΔCYT.Scer\UAS] under the control of Scer\GAL4[Act.PU] reduces the overgrowth of Sce[1] and of Pc[15] mutant eye discs. (Classen et al., 2009) Expression of dome[ΔCYT.Scer\UAS] under the control of Scer\GAL4[ey.PH] in a L[fee] mutant background restores eye size to normal. (Wang and Huang, 2009) The frequency of overgrowths observed in elB[3.3.1] noc[d64] double mutant ey clones is not affected when the JAK-STAT pathway is blocked by expression of dominant-negative dome[ΔCYT.Scer\UAS] (under the control of Scer\GAL4[Scer\FRT.Act5C]). (Luque and Milan, 2007) Co-expression of dome^{ΔCYT.Scer\UAS} completely suppresses the extra border follicle cells produced by expression of os^Scer\UAS.cZa under the control of Scer\GAL4^slbo.2.6. (Ghiglione et al., 2002) The penetrance and expressivity of the segmentation defects of embryos expressing dome^{ΔCYT.Scer\UAS} under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} is enhanced if the mothers are also heterozygous for hop^unspecified. (Brown et al., 2001)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to rescue	dome^{ΔCYT.Scer\UAS}/Scer\GAL4^unspecified fails to rescue dome^unspecified (Brown et al., 2001)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	dome^{ΔCYT.Scer\UAS} Dome^ΔCYT (Dostert et al., 2005) Dome Δcyt (Medioni and Noselli, 2005) DomeΔCYT (Classen et al., 2009)
Name Synonym
Secondary FlyBase IDs

References ( 21 )

Generate a list of	All references relating to this allele ( 21 )

List References by type	Research paper ( 19 ) Supplementary material ( 1 ) Personal communication to FlyBase ( 1 )
Recent research papers ( 2 )
	Luo and Sehgal, 2012, Cell 148(4): 765--779 Regulation of Circadian Behavioral Output via a MicroRNA-JAK/STAT Circuit. [FBrf0217497] Copf et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(19): 8059--8064 Cytokine signaling through the JAK/STAT pathway is required for long-term memory in Drosophila. [FBrf0213703] Show all research papers ...

Allele Dmel\domeΔCYT.Scer\UAS

Allele Dmel\dome^{ΔCYT.Scer\UAS}