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General Information
Symbol
Dmel\ago1
Species
D. melanogaster
Name
FlyBase ID
FBal0126458
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

C4248026T

Amino acid change:

Q1195term | ago-PA; Q1195term | ago-PB; Q1195term | ago-PC

Reported amino acid change:

Q1195term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: Q1195term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

ago1/agoΔ3-7 third instar larvae have tracheal defects, with the most prevalent phenotype being an approximate doubling of the number of cytoplasmic branches elaborated from multiple subtypes of terminal cells, including the lateral LH and LG terminal cells. Other defects include terminal branch tangles and the development of 'ringlet'-shaped ganglionic branches, which are seen in approximately 25% of larvae.

ago1/ago3 transheterozygous mutants develop through all embryonic stages but fail to hatch as L1 larvae.

ago1/Df(3L)Exel9000 transheterozygous mutants develop through all embryonic stages but fail to hatch as L1 larvae.

ago1/ago3 mutant embryos exhibit defects in the developing trachea. The earliest of these are interruptions or 'breaks' in the continuity of the tracheal lumen. Approximately 70% of these mutants display breaks throughout the tracheal system and are prominent in the dorsal trunk, the lateral trunk, and between dorsal branches of opposing tracheal placodes. The dorsal branches and ganglionic branches also appear to show misrouting. The second prominent tracheal phenotype (occurring in 25% of embryos) is excess lumen convolution through the primary and secondary branches. Mutants have approximately the same number of nuclei per dorsal trunk segment, indicating that the primary effect of ago loss in the dorsal trunk is not on cell number, but on fusion cell migration and fusion.

In ago1 homozygous embryos, 2% of hemisegments have two RP2 sibs and one RP2 neuron, while others have two RP2 neurons and one sib, rather than the one cell of each type seen in each segment in wild-type embryos.

Homozygous mutant clones in the wing disc and in the adult eye are consistently larger and contain more cells than their respective twin spots. Despite the faster rate of cell division in mutant cells, cells of homozygous wing disc clones are not decreased in size compared to control cells.

When ago1 mutant clones are induced in the ovary, some mutant cysts have only a single, large, highly polyploid germ-line cell.

Adult eyes mosaic for mutations in ago are composed mostly of mutant tissue, exhibiting a proliferative advantage over their wild-type twin-spots.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference

agoΔ3-7/ago1 has lethal phenotype, suppressible | partially by sima[+]/simaKG07607

Suppressor of
Statement
Reference

ago[+]/ago1 is a suppressor of female sterile | recessive phenotype of Mycdm-1

ago[+]/ago1 is a suppressor of decreased cell number | recessive phenotype of Mycdm-1

ago[+]/ago1 is a suppressor of decreased body size | recessive phenotype of Mycdm-1

ago[+]/ago1 is a suppressor of decreased body size | recessive | female phenotype of Mycdm-1

Phenotype Manifest In
Enhanced by
Suppressed by
Statement
Reference

Scer\GAL4GMR.PU, ago1, MycUAS.cZa has eye phenotype, suppressible by pufA459

agoΔ3-7/ago1 has tracheole phenotype, suppressible by sima[+]/simaKG07607

agoΔ3-7/ago1 has lateral H branch phenotype, suppressible by sima[+]/simaKG07607

agoΔ3-7/ago1 has tracheole phenotype, suppressible by bnl[+]/bnl00857

agoΔ3-7/ago1 has lateral H branch phenotype, suppressible by bnl[+]/bnl00857

ago3/ago1 has tracheal primordium phenotype, suppressible by trh10512/trh[+]

ago3/ago1 has tracheal fusion cell phenotype, suppressible by trh10512/trh[+]

ago3/ago1 has tracheal lumen phenotype, suppressible by btl[+]/btldev1

ago3/ago1 has tracheal primordium phenotype, suppressible by btl[+]/btldev1

ago3/ago1 has tracheal fusion cell phenotype, suppressible by btl[+]/btldev1

ago3/ago1 has tracheal lumen phenotype, suppressible by trh10512/trh[+]

Enhancer of
Statement
Reference

ago[+]/ago1 is an enhancer of eye phenotype of Scer\GAL4GMR.PU, pufEY03971

Suppressor of
Statement
Reference

ago[+]/ago1 is a suppressor of eye | somatic clone phenotype of COX5Atend

Additional Comments
Genetic Interactions
Statement
Reference

simaKG07607 dominantly delays the lethal phase of agoΔ3-7/ago1 transheterozygotes.

simaKG07607 dominantly suppresses the excess and overlapping terminal tracheal branching seen in agoΔ3-7/ago1 larvae.

bnl00857 dominantly suppresses the increase in number of terminal branches per lateral LH cell seen in ago1/agoΔ3-7 larvae.

An ago1 heterozygous background suppresses the CoVatend mutant clone adult eye phenotype.

A trh10512 heterozygous background dominantly suppresses the tracheal phenotypes found in ago1/ago3 mutants.

A btldev1 heterozygous background dominantly suppresses the tracheal phenotypes found in ago1/ago3 mutants.

A awdj2A4 heterozygous background strongly enhances both the penetrance (from 46% of embryos to greater than 80% of embryos) of the ago1/ago3 mutant dorsal trunk phenotype and its expressivity among affected embryos. These embryos also show a much more severe disruption of the entire tracheal system compared to ago1/ago3 mutants.

Heterozygosity for ago1 increase the female body length of dm1 flies by about 12%. The wings of dm1 ; ago1/+ adults are about 15% larger than those of dm1 adults. This suppression is due to an increase in the number of cells. dm1 ; ago1/+ females lay eggs that can give rise to viable larvae.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of agoScer\UAS.cMa in the developing tracheal system under the control of Scer\GAL4btl.PS in an ago1/ago3 mutant background significantly reduces the frequency of dorsal trunk breaks, along with other tracheal phenotypes.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
References (18)