FlyBase Allele Report: Scer\GAL4[c819]

General Information

Symbol

Scer\GAL4^c819

Species

S. cerevisiae

Name

FlyBase ID

FBal0126480

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

P{GawB}c819

Carried in Construct

Also Known As

C819-GAL4, c819

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

P{GawB}c819

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{GawB}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

Bolwig nerve

(Renn et al., 1999)

larval central nervous system | restricted

(Suster et al., 2003)

motor neuron | subset

(Suster et al., 2003)

symmetrical commissure | subset of larval brain | restricted

(Suster et al., 2003)

pupal stage P7 -- adult stage

adult ellipsoid body ring neuron R2

Comment: expression begins 32 hr APF

(Renn et al., 1999)

adult ellipsoid body ring neuron R4 | medial | subset

Comment: expression starts at 32 hr APF

(Renn et al., 1999)

ellipsoid body | restricted

Comment: expression starts at 32 hr APF ; expression is in inner part of ellipsoid body

(Renn et al., 1999)

Additional Information

Statement

Reference

Drives expression in the ellipsoid body, specifically in adult ellipsoid body ring neurons R2 and R4m.

(FlyBase Curators, 2017)

ScerGAL4^c819 drives adult expression in the ellipsoid body and suboesophageal ganglion.

(Bai and Sehgal, 2015)

ScerGAL4^c819 is expressed in some motorneurons along the dorsal midline in some segments of the larval VNC.

(Suster et al., 2003)

Pupal and adult expression is observed in R2 and medial R4 ring neurons, that arborize in the outer domain of the ellipsoid body.

(Renn et al., 1999)

Marker for

adult ellipsoid body ring neuron R2

(Renn et al., 1999)

adult ellipsoid body ring neuron R4m

(Renn et al., 1999)

ellipsoid body

(Renn et al., 1999)

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | third instar larval stage, with Ctet\tetX^TNT-LC.UAS

(Suster et al., 2003)

abnormal memory | adult stage | heat sensitive, with TrpA1^UAS.(B).cKa

(Zhang et al., 2013)

increased sleep | adult stage, with dco^GD1259

(Wang et al., 2022)

Phenotype Manifest In

Detailed Description

Statement

Reference

Inhibition of neural activity in Scer\GAL4^c819-expressing neurons (in which expression is suppressed in the ventral nerve cord using Scer\GAL80^tsh-GAL80) does not affect sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503). As in wild type flies, courtship defects are seen in response to (S,Z,Z)-CH503.

(Shankar et al., 2015)

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 28[o]C) of Scer\GAL4^c819 cells in adults significantly reduces anaesthesia-sensitive medium-term (3 h) aversive olfactory memory, with no effect on short term (2 min) memory or anaesthesia-resistant 3 h memory, compared to controls; activation of a smaller subset of Scer\GAL4^c819 (restricted using Scer\GAL80^Gad1) does not affect 3 h memory, compared to controls.

(Zhang et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^c819, dco^GD1259 has decreased sleep | adult stage phenotype, suppressible by per⁰¹

(Wang et al., 2022)

Phenotype Manifest In