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General Information
Symbol
Scer\GAL4ChAT.7.4
Species
S. cerevisiae
Name
FlyBase ID
FBal0126489
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Cha-GAL4, ChAT-GAL4, chaGAL4, ChaB19-GAL4, P{Cha-GAL4.7.4}, Cha-Gal4-19B, Cha(7.4)-Gal4, Cha7.4-Gal4, ChATGAL4, Choline acetyltransferase (Cha)-Gal4
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

A 7.4kb BamHI 5' flanking fragment from the "cholinergic" locus which encodes both ChAT and VAChT function) regulates expression of a GAL4 driver.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
ellipsoid body

Comment: strong expression

adult mushroom body

Comment: strong expression

eye

Comment: strong expression

Additional Information
Statement
Reference

Drives expression in cholinergic neurons in larvae and adults.

Scer\GAL4ChAT.7.4 and Scer\GAL4S3.Hug drive expression in larval Hugin neuron of the protocerebrum, larval Hugin neuron of the ventral nerve cord, larval Hugin neuron of the ventral nerve cord as well as, weakly, larval Hugin neuron of the corpus cardiacum.

Scer\GAL4ChAT.7.4 colocalizes with eve in EL neurons.

Scer\GAL4ChAT.7.4 labels a subset of neurons in the lamina, medulla, lobula and lobula plate. The labelled cells include: Tm1, Tm2, Tm4, Tm4a, Tm7, Tm8, Tm11, Tm12, Tm15, Tm22, Tm24, TmY2, TmY5, TmY9, TmY10, TmY11, TmY12, Mi2, Mi7, Mi11, Pm1, Pm1a, Mt4, Mt10, T2, T4, Tlp2, Tlp3, Lcn1, Lcn2, Lt6, Tm new 1, Tm new 2, Tm new 3, T new 1, mY new 1, Mt new 1, Mi new 1, Mi new 2, Mi new 3, Lcn new 1, Loi new 1 and lpi new 1.

Scer\GAL4ChAT.7.4 drives expression in the Rh5 and Rh6 photoreceptors of Bolwig organ in the larval optic neuropil.

Expression in the midgut is found in a distinct group of Dh31-expressing enteroendocrine cells located at the junction region of the anterior midgut and the acid-secreting portion of the larval midgut. This junction region occurs at a conspicuous and characteristic U-shaped bend in the gut. These are referred to as the "midgut junction Dh31-expressing cells". There is no overlap in expression between this marker and ChAT expression in the midgut.

Scer\GAL4ChAT.7.4 labels at least four LNd neurons and the Pdf negative s-LNv neuron. The four LNd neurons are also labeled by expression of per protein. Two of these four LNd neurons also co-express sNPF protein.

Most of the Scer\GAL4ChAT.7.4 expressing neurons do not colocalize with sNPF protein in the adult. However, there is colocalization in a subset of columnar neurons at the ventral base of the optic lobe with dense termination sites in the lateral protocerebrum.

Expression of Scer\GAL4ChAT.7.4 is limited to the ventral part of the ventral nerve cord.

In adults, Scer\GAL4VGlut-OK371 drives expression in thin neurites that innervate the beta gamma lobes, especially in the anterior region of the latter, and in the neuropils surrounding the lobes and peduncle. Co-localisation with sNPF protein is observed in a small number of cell bodies throughout the brain and in the neuronal axon tract anterior to the beta and gamma lobes. Larval expression was similar to the one in adults, with additional expression in processes that terminate in the calyx or that surround the dorsal tip of the vertical lobe.

 
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Optogenetic stimulation (using Npha\HRe.UAS.cBa.YFP with 593 nm (yellow) light activation) of Scer\GAL4ChAT.7.4 neurons reduces speed, distance and total duration of locomotion in adult flies, compared to controls.

Inhibition (using shi1.UAS at 31-32[o]C) of Scer\GAL4ChAT.7.4 cells in larvae results in paralysis. When inhibition is restricted to MARCM clones (using hs-FLP to remove a Gal80[ts] transgene by recombination with no heat shock induction), a range of phenotypes can occur, including slow movement, paralysis, defects in run initiation and/or maintenance, reduced or absent body wall peristalsis and lack of co-ordination, compared to controls; these phenotypes are correlated with the presence of interneuronal, rather than sensory, clones and clones that have arborizations in particular neuropil regions.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
Suppressor of
Statement
Reference

Jafrac1dsRNA.UAS/Scer\GAL4ChAT.7.4 is a suppressor | partially of short lived | conditional phenotype of Hayan1

IrcdsRNA.UAS/Scer\GAL4ChAT.7.4 is a suppressor | partially of short lived | conditional phenotype of Hayan1

NOT Suppressor of
Statement
Reference
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
NOT Enhancer of
Suppressor of
Statement
Reference

Scer\GAL4ChAT.7.4/Hsap\ADARB1UAS.cKa is a suppressor of retina | conditional phenotype of Adar5G1

Hsap\ADARUAS.p110/Scer\GAL4ChAT.7.4 is a suppressor | partially of retina | conditional phenotype of Adar5G1

NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (4)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Symbol Synonym
Gal4cha19b
Gal4chat19b
Scer\GAL4ChAT.7.4
Scer\GAL4Cha.7.4
Scer\GAL4Cha.PW
Name Synonyms
Secondary FlyBase IDs
    References (161)