FlyBase Allele Report: Scer\GAL4[ChAT.7.4]

ScerGAL4^ChAT.7.4 and ScerGAL4^S3.Hug drive expression in larval Hugin neuron of the protocerebrum, larval Hugin neuron of the ventral nerve cord, larval Hugin neuron of the ventral nerve cord as well as, weakly, larval Hugin neuron of the corpus cardiacum.

(Schlegel et al., 2016)

ScerGAL4^ChAT.7.4 colocalizes with eve in EL neurons.

(Heckscher et al., 2015)

ScerGAL4^Cha.7.4 labels a subset of neurons in the lamina, medulla, lobula and lobula plate. The labelled cells include: Tm1, Tm2, Tm4, Tm4a, Tm7, Tm8, Tm11, Tm12, Tm15, Tm22, Tm24, TmY2, TmY5, TmY9, TmY10, TmY11, TmY12, Mi2, Mi7, Mi11, Pm1, Pm1a, Mt4, Mt10, T2, T4, Tlp2, Tlp3, Lcn1, Lcn2, Lt6, Tm new 1, Tm new 2, Tm new 3, T new 1, mY new 1, Mt new 1, Mi new 1, Mi new 2, Mi new 3, Lcn new 1, Loi new 1 and lpi new 1.

(Varija Raghu et al., 2011)

ScerGAL4^Cha.7.4 drives expression in the Rh5 and Rh6 photoreceptors of Bolwig organ in the larval optic neuropil.

(Keene et al., 2011)

Expression in the midgut is found in a distinct group of Dh31-expressing enteroendocrine cells located at the junction region of the anterior midgut and the acid-secreting portion of the larval midgut. This junction region occurs at a conspicuous and characteristic U-shaped bend in the gut. These are referred to as the "midgut junction Dh31-expressing cells". There is no overlap in expression between this marker and ChAT expression in the midgut.

(Lajeunesse et al., 2010)

ScerGAL4^Cha.7.4 labels at least four LNd neurons and the Pdf negative s-LNv neuron. The four LNd neurons are also labeled by expression of per protein. Two of these four LNd neurons also co-express sNPF protein.

(Johard et al., 2009)

Most of the ScerGAL4^Cha.7.4 expressing neurons do not colocalize with sNPF protein in the adult. However, there is colocalization in a subset of columnar neurons at the ventral base of the optic lobe with dense termination sites in the lateral protocerebrum.

(Nässel et al., 2008)

Expression of ScerGAL4^Cha.7.4 is limited to the ventral part of the ventral nerve cord.

(Daniels et al., 2008)

In adults, ScerGAL4^VGlut-OK371 drives expression in thin neurites that innervate the beta gamma lobes, especially in the anterior region of the latter, and in the neuropils surrounding the lobes and peduncle. Co-localisation with sNPF protein is observed in a small number of cell bodies throughout the brain and in the neuronal axon tract anterior to the beta and gamma lobes. Larval expression was similar to the one in adults, with additional expression in processes that terminate in the calyx or that surround the dorsal tip of the vertical lobe.

(Johard et al., 2008)

ScerGAL4^ChAT.7.4 drives expression in cholinergic neurons from the onset of synaptogenesis.

(Baines et al., 2002)

Marker for

adult optic lobe

(Nässel et al., 2008)

cholinergic neuron

(Schlegel et al., 2016, Wittmann et al., 2001)

Reflects expression of

ChAT

(Schlegel et al., 2016, Heckscher et al., 2015, Chin et al., 2014, Chen et al., 2012, Keene et al., 2011, Varija Raghu et al., 2011, Boerner and Duch, 2010, Wu et al., 2010, Johard et al., 2009, Hamada et al., 2008, Johard et al., 2008, Kolodziejczyk et al., 2008, Zhou et al., 2008, Beramendi et al., 2007, Wittmann et al., 2001)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior, with TrpA1^RNAi.A.UAS, TrpA1^RNAi.B.UAS

(Hamada et al., 2008)

abnormal chemosensory behavior | third instar larval stage, with ChT^JF03028

(Hamid et al., 2021)

abnormal chemosensory behavior | third instar larval stage, with ChT^KK109385

(Hamid et al., 2021)

abnormal courtship behavior, with fipi^KK107002

(Fedotov et al., 2018)

abnormal gravitaxis | adult stage, with CG10738^HM05067

(Werner et al., 2024)

abnormal gravitaxis | adult stage, with CG10738^HMS01814

(Werner et al., 2024)

abnormal gravitaxis | adult stage, with CG31183^HMJ22232

(Werner et al., 2024)

abnormal gravitaxis | adult stage, with Gucy2d^GD14677

(Werner et al., 2024)

abnormal gravitaxis | adult stage, with Gyc89Da^HM05246

(Werner et al., 2024)

abnormal gravitaxis | adult stage | progressive, with Gucy2d^HM05010

(Werner et al., 2024)

abnormal gravitaxis | adult stage | progressive, with Gucy2d^KK110863

(Werner et al., 2024)

abnormal gravitaxis | adult stage | progressive, with nompB^JF03080

(Werner et al., 2024)

abnormal grooming behavior, with Mmus\Prnp^P101L.UAS

(Gavin et al., 2006)

abnormal heat stress response | adult stage, with sei^JF01474

(Hill et al., 2019)

abnormal learning | adult stage, with VAChT^UAS.cKa

(Showell et al., 2020)

abnormal learning | third instar larval stage, with ChT^JF03028

(Hamid et al., 2021)

abnormal learning | third instar larval stage, with ChT^KK109385

(Hamid et al., 2021)

abnormal locomotor behavior, with Hsap\APP^{Aβ42.UAS.Tag:SS(rPENK)}

(Iijima-Ando et al., 2009)

abnormal locomotor behavior, with Mmus\Prnp^{P101L.UAS.Tag:3F4}

(Choi et al., 2010)

abnormal locomotor behavior | adult stage, with COX7A^KK110870

(Yalgin et al., 2020)

abnormal locomotor behavior | adult stage, with EndoU^GD3933

(Laneve et al., 2017)

abnormal locomotor behavior | adult stage, with Hsap\MECP2^UAS.FL

(Cukier et al., 2008)

abnormal locomotor behavior | adult stage, with Mmus\Prnp^{P101L.UAS.cMa}

(Murali et al., 2014)

abnormal locomotor behavior | adult stage, with Mmus\Prnp^P101L.UAS

(Gavin et al., 2006)

abnormal locomotor behavior | adult stage, with Mmus\Prnp^UAS.cMa

(Murali et al., 2014)

abnormal locomotor behavior | adult stage | conditional, with Npha\HR^{eNpHR2.0.UAS.cBa.YFP}

(Liu et al., 2016)

abnormal locomotor behavior | adult stage | drug conditional, with Rnor\P2rx2^UAS.cLa

(Lima and Miesenbock, 2005)

abnormal locomotor behavior | adult stage | progressive, with Hsap\APP^{Aβ42.UAS.Tag:SS(rPENK)}

(Ling et al., 2009)

abnormal locomotor behavior | adult stage | progressive, with VAChT^UAS.cKa

(Showell et al., 2020)

abnormal locomotor behavior | larval stage, with ChT^KK109385

(Hamid et al., 2019)

abnormal locomotor behavior | larval stage | heat sensitive, with shi^1.UAS

(Kitamoto, 2001)

abnormal locomotor behavior | larval stage | somatic clone | heat sensitive, with shi^1.UAS

(Iyengar et al., 2011)

abnormal locomotor behavior | third instar larval stage, with Mmus\Prnp^P101L.UAS

(Gavin et al., 2006)

abnormal locomotor behavior | third instar larval stage, with TEV\NIa^{TEVp.UAS.Tag:V5,Tag:NLS(SV40-largeT)}, vtd^{550TEV.tub.Tag:CS(TEVp),Tag:MYC}, vtd^ex15

(Pauli et al., 2008)

abnormal mating behavior | female, with SP^{UAS.Tag:M(hGGT1)}

(Rezaval et al., 2012)

abnormal memory, with Rcom\RT^A-cs2.UAS

(Chen et al., 2012)

abnormal memory, with Rcom\RT^A-cs2.UAS, Scer\GAL80^Mef2.mb247

(Chen et al., 2012)

abnormal memory | adult stage, with VAChT^UAS.cKa

(Showell et al., 2020)

abnormal neuroanatomy, with Fas2^UAS.cLb, Scer\GAL4^eve.RRK

(Baines et al., 2002)

abnormal neuroanatomy, with fra::robo1^{UAS.FR.Tag:MYC}

(Mauss et al., 2009)

abnormal neuroanatomy, with fra::robo1^{UAS.RF.Tag:MYC}

(Mauss et al., 2009)

abnormal neuroanatomy, with Hsap\MAPT^R406W.UAS

(Nishimura et al., 2004)

abnormal neuroanatomy, with Mdoa\Ace^UAS.cKa

(Kim et al., 2011)

abnormal neuroanatomy | adult stage, with Ephrin^HMS01289

(Liu et al., 2017)

abnormal neurophysiology, with Dys^{RNAi.Dp186.UAS}

(Fradkin et al., 2008)

abnormal neurophysiology, with Fas2^UAS.cLb

(Baines et al., 2002)

abnormal neurophysiology, with Mmus\Prnp^{P101L.UAS.cMa}

(Murali et al., 2014)

abnormal neurophysiology, with tkv^{Q199D.UAS.Tag:HA}

(Baines, 2004)

abnormal neurophysiology | adult stage, with Gucy2d^HM05010

(Werner et al., 2024)

abnormal neurophysiology | adult stage, with nompB^JF03080

(Werner et al., 2024)

abnormal neurophysiology | heat sensitive, with shi^1.UAS

(Rajaram et al., 2005)

abnormal neurophysiology | larval stage, with Rnor\P2rx2^UAS.cLa

(Lima and Miesenbock, 2005)

abnormal neurophysiology | larval stage, with stas^GD3415

(Lotti et al., 2012)

abnormal neurophysiology | larval stage | drug conditional, with Rnor\P2rx2^UAS.cLa

(Lima and Miesenbock, 2005)

abnormal neurophysiology | third instar larval stage, with Syx1A^T254I.UAS

(Vernon et al., 2018)

abnormal neurophysiology | third instar larval stage, with VAChT^-Q.UAS

(Vernon et al., 2019)

abnormal neurophysiology | third instar larval stage, with VAChT^UAS.cCa

(Vernon et al., 2019)

abnormal sleep, with Alk^GD42

(Bai and Sehgal, 2015)

abnormal stress response, with Rac1^N17.UAS, Scer\GAL80^ts.αTub84B

(Shuai et al., 2011)

bang sensitive, with jus^JF03192

(Horne et al., 2017)

chemical resistant, with VAChT^-Q.UAS

(Vernon et al., 2018)

chemical resistant, with VAChT^UAS.cSa

(Sluder et al., 2012)

chemical resistant, with VAChT^Y49N.UAS

(Sluder et al., 2012)

decreased cell number | adult stage, with SmB^HM05097

(Hsieh et al., 2019)

decreased efficacy of learning | adult stage, with LIMK1^UAS.Tag:HA

(Zhuravlev et al., 2024)

decreased rate of movement | larval stage | somatic clone | heat sensitive, with shi^1.UAS

(Iyengar et al., 2011)

female sterile, with dati^KK104525

(Schinaman et al., 2014)

flightless, with Mmus\Prnp^P101L.UAS

(Gavin et al., 2006)

increased cell number | larval stage, with ChAT^HMC05021

(Bazzi et al., 2023)

increased cell size | adult stage, with Hsap\APP^{Aβ42.UAS.Tag:SS(rPENK)}

(Ling et al., 2009)

increased rate of movement | drug conditional, with Rab10^{T23N.UASp.YFP}

(Vanderwerf et al., 2015)

lethal, with Gyc89Db^HMJ22088

(Werner et al., 2024)

lethal | larval stage, with acj6^1,4.UAS

(Lee and Salvaterra, 2002)

lethal | pupal stage, with acj6^1,4.UAS

(Lee and Salvaterra, 2002)

lethal - all die before end of first instar larval stage | heat sensitive, with shi^1.UAS

(Kitamoto, 2001)

lethal - all die before end of pupal stage, with Dop1R2^UAS.cHa

(Cassar et al., 2015)

lethal - all die during embryonic stage, with Hsap\KCNT1^G288S.UAS

(Hussain et al., 2024)

lethal - all die during embryonic stage, with Hsap\KCNT1^R398Q.UAS

(Hussain et al., 2024)

lethal - all die during embryonic stage, with Hsap\KCNT1^R928C.UAS

(Hussain et al., 2024)

paralytic | adult stage | drug conditional, with Rnor\P2rx2^UAS.cLa

(Lima and Miesenbock, 2005)

paralytic | adult stage | heat sensitive, with shi^1.UAS

(Kitamoto, 2001)

paralytic | cold sensitive, with jus^JF03192

(Dean et al., 2018)

paralytic | heat sensitive, with shi^1.UAS

(Beramendi et al., 2007, Schmid et al., 2006)

paralytic | larval stage | heat sensitive, with shi^1.UAS

(Iyengar et al., 2011)

paralytic | larval stage | somatic clone | heat sensitive, with shi^1.UAS

(Iyengar et al., 2011)

paralytic | third instar larval stage, with pum^KK109048

(Lin et al., 2017)

paralytic | third instar larval stage, with pum^UAS.cSa

(Lin et al., 2017)

paralytic | third instar larval stage | heat sensitive, with shi^K44A.UAS

(Berni et al., 2012)

partially lethal, with Mmus\Prnp^P101L.UAS

(Gavin et al., 2006)

short lived, with Atg5^RNAi.UAS

(Ling et al., 2009)

short lived, with EndoU^GD3933

(Laneve et al., 2017)

short lived, with Hsap\APP^{Aβ42.UAS.Tag:SS(rPENK)}

(Ling et al., 2009)

short lived, with Mmus\Prnp^P101L.UAS

(Gavin et al., 2006)

short lived, with Rnor\P2rx2^UAS.cLa

(Lima and Miesenbock, 2005)

short lived, with VAChT^-Q.UAS

(Vernon et al., 2018)

short lived, with VAChT^UAS.cKa

(Showell et al., 2020)

short lived | female, with sgg^UAS.cBa

(Trostnikov et al., 2019)

short lived | male, with sgg^UAS.cBa

(Trostnikov et al., 2019)

some die during first instar larval stage | heat sensitive, with shi^1.UAS

(Kitamoto, 2001)

some die during larval stage, with Mmus\Prnp^P101L.UAS

(Gavin et al., 2006)

uncoordinated | larval stage | somatic clone | heat sensitive, with shi^1.UAS

(Iyengar et al., 2011)

viable, with Adar^3.4.S.UAS, Adar^5G1

(Keegan et al., 2005)

viable, with cbc^HMC06558

(Schmidt et al., 2022)

viable, with Hsap\KCNT1^UAS.cHa

(Hussain et al., 2024)

visible, with Nup44A^EP2417

(Ghosh and Feany, 2004)

visible, with Tpr2^UAS.cKa

(Ghosh and Feany, 2004)

visible | adult stage, with EndoU^GD3933

(Laneve et al., 2017)

Phenotype Manifest In

adult brain, with Hsap\ATXN1^82.UAS

(Ghosh and Feany, 2004)

adult brain | progressive, with Mmus\Prnp^P101L.UAS

(Gavin et al., 2006)

adult central complex, with Hsap\MAPT^R406W.UAS

(Wittmann et al., 2001)

adult cuticle, with EndoU^GD3933

(Laneve et al., 2017)

adult optic lobe | adult stage | progressive, with Mmus\Prnp^P101L.UAS

(Gavin et al., 2006)

adult thorax, with EndoU^GD3933

(Laneve et al., 2017)

autophagosome | adult stage, with Hsap\APP^{Aβ42.UAS.Tag:SS(rPENK)}

(Ling et al., 2009)

axon | adult stage, with Ephrin^HMS01289

(Liu et al., 2017)

axon terminus | adult stage, with loaf^Δ33, loaf^UAS.Tag:HA

(Douthit et al., 2021)

cholinergic neuron, with fra::robo1^{UAS.FR.Tag:MYC}

(Mauss et al., 2009)

cholinergic neuron, with fra::robo1^{UAS.RF.Tag:MYC}

(Mauss et al., 2009)

cholinergic neuron, with Hsap\ATXN1^82.UAS

(Ghosh and Feany, 2004)

cholinergic neuron | adult stage, with SmB^HM05097

(Hsieh et al., 2019)

cilium | adult stage, with nompB^JF03080

(Werner et al., 2024)

embryonic/larval hemocyte | increased number | larval stage, with ChAT^HMC05021

(Bazzi et al., 2023)

embryonic/larval hypodermal muscle cell | somatic clone | heat sensitive, with shi^1.UAS

(Iyengar et al., 2011)

embryonic/larval motor neuron | third instar larval stage, with Syx1A^T254I.UAS

(Vernon et al., 2018)

embryonic/larval motor neuron | third instar larval stage, with VAChT^-Q.UAS

(Vernon et al., 2019)

embryonic/larval motor neuron | third instar larval stage, with VAChT^UAS.cCa

(Vernon et al., 2019)

eye, with Nup44A^EP2417

(Ghosh and Feany, 2004)

eye, with Tpr2^UAS.cKa

(Ghosh and Feany, 2004)

Johnston organ neuron, with Gucy2d^HM05010

(Werner et al., 2024)

Johnston organ neuron, with nompB^JF03080

(Werner et al., 2024)

lamina, with Hsap\MAPT^R406W.UAS

(Wittmann et al., 2001)

lamina, with Hsap\MAPT^UAS.cWa

(Wittmann et al., 2001)

lamina, with SmB^HM05097

(Hsieh et al., 2019)

lamina monopolar neuron L4 | adult stage, with Ephrin^HMS01289

(Liu et al., 2017)

larval DA1 motor neuron, with Fas2^UAS.cLb

(Baines et al., 2002)

larval RP2 motor neuron, with Fas2^UAS.cLb

(Baines et al., 2002)

larval VL3/4 motor neuron, with Fas2^UAS.cLb, Scer\GAL4^eve.RRK

(Baines et al., 2002)

medulla | adult stage, with Ephrin^HMS01289

(Liu et al., 2017)

neuron, with Hsap\APP^{Aβ42.UAS.Tag:SS(rPENK)}

(Iijima-Ando et al., 2009)

neuron, with Hsap\MAPT^R406W.UAS

(Nishimura et al., 2004)

neuron | adult stage, with Hsap\APP^{Aβ42.UAS.Tag:SS(rPENK)}

(Ling et al., 2009)

neuropil | adult stage, with Hsap\APP^{Aβ42.UAS.Tag:SS(rPENK)}

(Ling et al., 2009)

nuclear membrane | adult stage, with Hsap\APP^{Aβ42.UAS.Tag:SS(rPENK)}

(Ling et al., 2009)

organelle membrane | adult stage, with Hsap\APP^{Aβ42.UAS.Tag:SS(rPENK)}

(Ling et al., 2009)

outer medulla | adult stage, with loaf^Δ33, loaf^UAS.Tag:HA

(Douthit et al., 2021)

photoreceptor cell R7 | adult stage, with loaf^Δ33, loaf^UAS.Tag:HA

(Douthit et al., 2021)

plasma membrane | adult stage, with Hsap\APP^{Aβ42.UAS.Tag:SS(rPENK)}

(Ling et al., 2009)

ptilinum, with EndoU^GD3933

(Laneve et al., 2017)

scutellar bristle, with EndoU^GD3933

(Laneve et al., 2017)

symmetrical commissure, with fra::robo1^{UAS.FR.Tag:MYC}

(Mauss et al., 2009)

symmetrical commissure, with fra::robo1^{UAS.RF.Tag:MYC}

(Mauss et al., 2009)

synapse, with Fas2^UAS.cLb, Scer\GAL4^eve.RRK

(Baines et al., 2002)

synapse, with Mdoa\Ace^UAS.cKa

(Kim et al., 2011)

vesicle | adult stage, with Hsap\APP^{Aβ42.UAS.Tag:SS(rPENK)}

(Ling et al., 2009)

wing blade, with EndoU^GD3933

(Laneve et al., 2017)

Detailed Description

Statement

Reference

Optogenetic stimulation (using Npha\HR^{e.UAS.cBa.YFP} with 593 nm (yellow) light activation) of Scer\GAL4^ChAT.7.4 neurons reduces speed, distance and total duration of locomotion in adult flies, compared to controls.

(Liu et al., 2016)

Inhibition (using shi^1.UAS at 31-32[o]C) of Scer\GAL4^ChAT.7.4 cells in larvae results in paralysis. When inhibition is restricted to MARCM clones (using hs-FLP to remove a Gal80[ts] transgene by recombination with no heat shock induction), a range of phenotypes can occur, including slow movement, paralysis, defects in run initiation and/or maintenance, reduced or absent body wall peristalsis and lack of co-ordination, compared to controls; these phenotypes are correlated with the presence of interneuronal, rather than sensory, clones and clones that have arborizations in particular neuropil regions.

(Iyengar et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Hsap\APP^{Aβ42.UAS.Tag:SS(rPENK)}, Scer\GAL4^ChAT.7.4 has abnormal locomotor behavior phenotype, enhanceable by Adcy1^rut-1

(Iijima-Ando et al., 2009)

Hsap\MAPT^R406W.UAS, Scer\GAL4^ChAT.7.4 has abnormal neuroanatomy phenotype, enhanceable by par-1^UAS.cSa, Scer\GAL4^ChAT.7.4

(Nishimura et al., 2004)

Suppressed by

Statement

Reference

Hsap\APP^{Aβ42.UAS.Tag:SS(rPENK)}, Scer\GAL4^ChAT.7.4 has abnormal locomotor behavior phenotype, suppressible by Pde4^dnc-1

(Iijima-Ando et al., 2009)

Hsap\MECP2^UAS.FL, Scer\GAL4^ChAT.7.4 has abnormal locomotor behavior | adult stage phenotype, suppressible by osa⁰⁰⁰⁹⁰/osa[+]

(Cukier et al., 2008)

Hsap\MECP2^UAS.FL, Scer\GAL4^ChAT.7.4 has abnormal locomotor behavior | adult stage phenotype, suppressible by trc^UAS.cUa, Scer\GAL4^ChAT.7.4

(Cukier et al., 2008)

NOT suppressed by

Statement

Reference

Mmus\Prnp^{P101L.UAS.Tag:3F4}, Scer\GAL4^ChAT.7.4 has abnormal locomotor behavior phenotype, non-suppressible by BacA\p35^UAS.cUa, Scer\GAL4^ChAT.7.4

(Choi et al., 2010)

Enhancer of

Statement

Reference

SLO2^DN.UAS/Scer\GAL4^ChAT.7.4 is an enhancer of bang sensitive | adult stage | heat sensitive phenotype of eas²

(Byers et al., 2021)

SLO2^DN.UAS/Scer\GAL4^ChAT.7.4 is an enhancer of bang sensitive | adult stage | heat sensitive phenotype of jus^iso7.8

(Byers et al., 2021)

SLO2^DN.UAS/Scer\GAL4^ChAT.7.4 is an enhancer of bang sensitive | adult stage | heat sensitive phenotype of para^bss1

(Byers et al., 2021)

pum^KK109048/Scer\GAL4^ChAT.7.4 is an enhancer of paralytic | adult stage phenotype of para^bss1

(Lin et al., 2017)

pum^KK109048/Scer\GAL4^ChAT.7.4 is an enhancer of paralytic | third instar larval stage phenotype of para^bss1

(Lin et al., 2017)

par-1^UAS.cSa, Scer\GAL4^ChAT.7.4 is an enhancer of abnormal neuroanatomy phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^ChAT.7.4

(Nishimura et al., 2004)

Suppressor of

Statement

Reference

Scer\GAL4^ChAT.7.4/Hsap\ADARB1^UAS.cKa is a suppressor | partially of abnormal locomotor behavior | adult stage phenotype of Adar^E374A

(Deng et al., 2020)

Hsap\SOD1^UAS.cWa/Scer\GAL4^ChAT.7.4 is a suppressor of abnormal neurophysiology | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Hsap\SOD1^UAS.cWa/Scer\GAL4^ChAT.7.4 is a suppressor of abnormal locomotor behavior | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Hsap\SOD1^UAS.cWa/Scer\GAL4^ChAT.7.4 is a suppressor of abnormal neuroanatomy | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

pum^UAS.cSa/Scer\GAL4^ChAT.7.4 is a suppressor of paralytic | adult stage phenotype of para^bss1

(Lin et al., 2017)

pum^UAS.cSa/Scer\GAL4^ChAT.7.4 is a suppressor of paralytic | third instar larval stage phenotype of para^bss1

(Lin et al., 2017)

Toll-7^{ΔLRR.UAS.Tag:HA}/Scer\GAL4^ChAT.7.4 is a suppressor of some die during embryonic stage | heat sensitive phenotype of Toll-6²⁶, Toll-7^P8

(McIlroy et al., 2013)

Toll-7^{C993Y.UAS.Tag:FLAG}/Scer\GAL4^ChAT.7.4 is a suppressor of some die during embryonic stage | heat sensitive phenotype of Toll-6²⁶, Toll-7^P8

(McIlroy et al., 2013)

Toll-6^{ΔLRR.UAS.Tag:HA}/Scer\GAL4^ChAT.7.4 is a suppressor of some die during embryonic stage | heat sensitive phenotype of Toll-6²⁶, Toll-7^P8

(McIlroy et al., 2013)

Scer\GAL4^ChAT.7.4 is a suppressor of abnormal neurophysiology | larval stage phenotype of Smn^X7

(Lotti et al., 2012)

Scer\GAL4^ChAT.7.4 is a suppressor of abnormal size | larval stage phenotype of Smn^X7

(Lotti et al., 2012)

Prx2^RNAi.UAS/Scer\GAL4^ChAT.7.4 is a suppressor | partially of short lived | conditional phenotype of Hayan¹

(Nam et al., 2012)

Irc^RNAi.UAS/Scer\GAL4^ChAT.7.4 is a suppressor | partially of short lived | conditional phenotype of Hayan¹

(Nam et al., 2012)

Klp64D^UAS.GFP/Scer\GAL4^ChAT.7.4 is a suppressor | partially of abnormal neurophysiology phenotype of Kap3^V5

(Jana et al., 2011)

Klp64D^UAS.GFP/Scer\GAL4^ChAT.7.4 is a suppressor | partially of abnormal neuroanatomy phenotype of Kap3^V5

(Jana et al., 2011)

Klp64D^UAS.GFP/Scer\GAL4^ChAT.7.4 is a suppressor of abnormal neurophysiology phenotype of Kap3^V6

(Jana et al., 2011)

Klp64D^UAS.GFP/Scer\GAL4^ChAT.7.4 is a suppressor of abnormal neuroanatomy phenotype of Kap3^V6

(Jana et al., 2011)

Scer\GAL4^ChAT.7.4/Hsap\ADARB1^UAS.cKa is a suppressor of abnormal locomotor behavior | adult stage phenotype of Adar^5G1

(Keegan et al., 2011)

Scer\GAL4^ChAT.7.4/Hsap\ADARB1^UAS.cKa is a suppressor of abnormal neuroanatomy | adult stage | conditional phenotype of Adar^5G1

(Keegan et al., 2011)

Hsap\ADAR^UAS.p110/Scer\GAL4^ChAT.7.4 is a suppressor | partially of abnormal neuroanatomy | adult stage | conditional phenotype of Adar^5G1

(Keegan et al., 2011)

Scer\GAL4^ChAT.7.4/Hsap\ADARB1^UAS.cKa is a suppressor of abnormal locomotor behavior | adult stage phenotype of Adar^1F4

(Keegan et al., 2011)

Atg5^RNAi.UAS/Scer\GAL4^ChAT.7.4 is a suppressor of short lived phenotype of Hsap\APP^{Aβ42.UAS.Tag:SS(rPENK)}

(Ling et al., 2009)

trc^UAS.cUa, Scer\GAL4^ChAT.7.4 is a suppressor of abnormal locomotor behavior | adult stage phenotype of Hsap\MECP2^UAS.FL, Scer\GAL4^ChAT.7.4

(Cukier et al., 2008)

esg^EP684/Scer\GAL4^ChAT.7.4 is a suppressor of bang sensitive phenotype of eas²

(Hekmat-Scafe et al., 2005)

NOT Suppressor of

Statement

Reference

Scer\GAL4^ChAT.7.4 is a non-suppressor of abnormal locomotor behavior | larval stage phenotype of Smn^X7

(Lotti et al., 2012)

Scer\GAL4^ChAT.7.4 is a non-suppressor of abnormal locomotor rhythm | larval stage phenotype of Smn^X7

(Lotti et al., 2012)

Klp68D^UAS.YFP/Scer\GAL4^ChAT.7.4 is a non-suppressor of abnormal neurophysiology phenotype of Kap3^V5

(Jana et al., 2011)

Klp68D^UAS.YFP/Scer\GAL4^ChAT.7.4 is a non-suppressor of abnormal neurophysiology phenotype of Kap3^V6

(Jana et al., 2011)

Hsap\ADAR^UAS.p110/Scer\GAL4^ChAT.7.4 is a non-suppressor of abnormal locomotor behavior | adult stage phenotype of Adar^5G1

(Keegan et al., 2011)

Hsap\ADAR^UAS.p150/Scer\GAL4^ChAT.7.4 is a non-suppressor of abnormal locomotor behavior | adult stage phenotype of Adar^5G1

(Keegan et al., 2011)

Hsap\ADARB1^G.UAS/Scer\GAL4^ChAT.7.4 is a non-suppressor of abnormal locomotor behavior | adult stage phenotype of Adar^5G1

(Keegan et al., 2011)

Hsap\ADAR^UAS.p150/Scer\GAL4^ChAT.7.4 is a non-suppressor of abnormal neuroanatomy | adult stage | conditional phenotype of Adar^5G1

(Keegan et al., 2011)

Hsap\ADAR^UAS.p110/Scer\GAL4^ChAT.7.4 is a non-suppressor of abnormal locomotor behavior | adult stage phenotype of Adar^1F4

(Keegan et al., 2011)

Hsap\ADAR^UAS.p150/Scer\GAL4^ChAT.7.4 is a non-suppressor of abnormal locomotor behavior | adult stage phenotype of Adar^1F4

(Keegan et al., 2011)

BacA\p35^UAS.cUa, Scer\GAL4^ChAT.7.4 is a non-suppressor of abnormal locomotor behavior phenotype of Mmus\Prnp^{P101L.UAS.Tag:3F4}, Scer\GAL4^ChAT.7.4

(Choi et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Hsap\MAPT^R406W.UAS, Scer\GAL4^ChAT.7.4 has neuron phenotype, enhanceable by par-1^UAS.cSa, Scer\GAL4^ChAT.7.4

(Nishimura et al., 2004)

NOT Enhanced by

Statement

Reference

Hsap\ATXN1^82.UAS, Scer\GAL4^ChAT.7.4 has adult brain phenotype, non-enhanceable by Sirt1^EP2300, Scer\GAL4^ChAT.7.4

(Ghosh and Feany, 2004)

Hsap\ATXN1^82.UAS, Scer\GAL4^ChAT.7.4 has adult brain phenotype, non-enhanceable by tara^EP3463, Scer\GAL4^ChAT.7.4

(Ghosh and Feany, 2004)

Hsap\ATXN1^82.UAS, Scer\GAL4^ChAT.7.4 has adult brain phenotype, non-enhanceable by pum^EP3461, Scer\GAL4^ChAT.7.4

(Ghosh and Feany, 2004)

Hsap\ATXN1^82.UAS, Scer\GAL4^ChAT.7.4 has adult brain phenotype, non-enhanceable by Hsc70-4^K71S.UAS, Scer\GAL4^ChAT.7.4

(Ghosh and Feany, 2004)

Suppressed by

Statement

Reference

Hsap\ATXN1^82.UAS, Scer\GAL4^ChAT.7.4 has adult brain phenotype, suppressible by Hsap\HSPA1L^UAS.cWa, Scer\GAL4^ChAT.7.4

(Ghosh and Feany, 2004)

Hsap\ATXN1^82.UAS, Scer\GAL4^ChAT.7.4 has adult brain phenotype, suppressible by BacA\p35^UAS.cUa, Scer\GAL4^ChAT.7.4

(Ghosh and Feany, 2004)

Hsap\ATXN1^82.UAS, Scer\GAL4^ChAT.7.4 has adult brain phenotype, suppressible by DnaJ-1^UAS.cKa, Scer\GAL4^ChAT.7.4

(Ghosh and Feany, 2004)

Hsap\ATXN1^82.UAS, Scer\GAL4^ChAT.7.4 has adult brain phenotype, suppressible by Diap1^UAS.cLa, Scer\GAL4^ChAT.7.4

(Ghosh and Feany, 2004)

NOT suppressed by

Statement

Reference

Hsap\ATXN1^82.UAS, Scer\GAL4^ChAT.7.4 has adult brain phenotype, non-suppressible by Hsc70-4^K71S.UAS, Scer\GAL4^ChAT.7.4

(Ghosh and Feany, 2004)

Hsap\ATXN1^82.UAS, Scer\GAL4^ChAT.7.4 has adult brain phenotype, non-suppressible by Sirt1^EP2300, Scer\GAL4^ChAT.7.4

(Ghosh and Feany, 2004)

Hsap\ATXN1^82.UAS, Scer\GAL4^ChAT.7.4 has adult brain phenotype, non-suppressible by tara^EP3463, Scer\GAL4^ChAT.7.4

(Ghosh and Feany, 2004)

Hsap\ATXN1^82.UAS, Scer\GAL4^ChAT.7.4 has adult brain phenotype, non-suppressible by pum^EP3461, Scer\GAL4^ChAT.7.4

(Ghosh and Feany, 2004)

Enhancer of

Statement

Reference

par-1^UAS.cSa, Scer\GAL4^ChAT.7.4 is an enhancer of neuron phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^ChAT.7.4

(Nishimura et al., 2004)

NOT Enhancer of

Statement

Reference

Sirt1^EP2300, Scer\GAL4^ChAT.7.4 is a non-enhancer of adult brain phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^ChAT.7.4

(Ghosh and Feany, 2004)

tara^EP3463, Scer\GAL4^ChAT.7.4 is a non-enhancer of adult brain phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^ChAT.7.4

(Ghosh and Feany, 2004)

pum^EP3461, Scer\GAL4^ChAT.7.4 is a non-enhancer of adult brain phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^ChAT.7.4

(Ghosh and Feany, 2004)

Hsc70-4^K71S.UAS, Scer\GAL4^ChAT.7.4 is a non-enhancer of adult brain phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^ChAT.7.4

(Ghosh and Feany, 2004)

Suppressor of

Statement

Reference

Hsap\SOD1^UAS.cWa/Scer\GAL4^ChAT.7.4 is a suppressor of embryonic/larval neuromuscular junction | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Hsap\SOD1^UAS.cWa/Scer\GAL4^ChAT.7.4 is a suppressor of NMJ bouton | increased number | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Scer\GAL4^ChAT.7.4 is a suppressor of embryonic/larval neuromuscular junction | larval stage phenotype of Smn^X7

(Lotti et al., 2012)

Scer\GAL4^ChAT.7.4 is a suppressor of larval somatic muscle cell | larval stage phenotype of Smn^X7

(Lotti et al., 2012)

Klp64D^UAS.GFP/Scer\GAL4^ChAT.7.4 is a suppressor | partially of trichoid sensillum of antennal segment 3 phenotype of Kap3^V5

(Jana et al., 2011)

Klp64D^UAS.GFP/Scer\GAL4^ChAT.7.4 is a suppressor | partially of basiconic sensillum of antennal segment 3 phenotype of Kap3^V5

(Jana et al., 2011)

Klp64D^UAS.GFP/Scer\GAL4^ChAT.7.4 is a suppressor | partially of cilium phenotype of Kap3^V5

(Jana et al., 2011)

Klp64D^UAS.GFP/Scer\GAL4^ChAT.7.4 is a suppressor of trichoid sensillum of antennal segment 3 phenotype of Kap3^V6

(Jana et al., 2011)

Klp64D^UAS.GFP/Scer\GAL4^ChAT.7.4 is a suppressor of basiconic sensillum of antennal segment 3 phenotype of Kap3^V6

(Jana et al., 2011)

Klp64D^UAS.GFP/Scer\GAL4^ChAT.7.4 is a suppressor of cilium phenotype of Kap3^V6

(Jana et al., 2011)

Scer\GAL4^ChAT.7.4/Hsap\ADARB1^UAS.cKa is a suppressor of retina | conditional phenotype of Adar^5G1

(Keegan et al., 2011)

Scer\GAL4^ChAT.7.4/Hsap\ADARB1^UAS.cKa is a suppressor of adult mushroom body | conditional phenotype of Adar^5G1

(Keegan et al., 2011)

Hsap\ADAR^UAS.p110/Scer\GAL4^ChAT.7.4 is a suppressor | partially of retina | conditional phenotype of Adar^5G1

(Keegan et al., 2011)

Hsap\ADAR^UAS.p110/Scer\GAL4^ChAT.7.4 is a suppressor | partially of adult mushroom body | conditional phenotype of Adar^5G1

(Keegan et al., 2011)

Hsap\HSPA1L^UAS.cWa, Scer\GAL4^ChAT.7.4 is a suppressor of adult brain phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^ChAT.7.4

(Ghosh and Feany, 2004)

BacA\p35^UAS.cUa, Scer\GAL4^ChAT.7.4 is a suppressor of adult brain phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^ChAT.7.4

(Ghosh and Feany, 2004)

DnaJ-1^UAS.cKa, Scer\GAL4^ChAT.7.4 is a suppressor of adult brain phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^ChAT.7.4

(Ghosh and Feany, 2004)

Diap1^UAS.cLa, Scer\GAL4^ChAT.7.4 is a suppressor of adult brain phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^ChAT.7.4

(Ghosh and Feany, 2004)

NOT Suppressor of

Statement

Reference

Klp68D^UAS.YFP/Scer\GAL4^ChAT.7.4 is a non-suppressor of trichoid sensillum of antennal segment 3 phenotype of Kap3^V6

(Jana et al., 2011)

Klp68D^UAS.YFP/Scer\GAL4^ChAT.7.4 is a non-suppressor of basiconic sensillum of antennal segment 3 phenotype of Kap3^V6

(Jana et al., 2011)

Klp68D^UAS.YFP/Scer\GAL4^ChAT.7.4 is a non-suppressor of cilium phenotype of Kap3^V6

(Jana et al., 2011)

Hsap\ADAR^UAS.p150/Scer\GAL4^ChAT.7.4 is a non-suppressor of retina | conditional phenotype of Adar^5G1

(Keegan et al., 2011)

Hsap\ADAR^UAS.p150/Scer\GAL4^ChAT.7.4 is a non-suppressor of adult mushroom body | conditional phenotype of Adar^5G1

(Keegan et al., 2011)

Hsc70-4^K71S.UAS, Scer\GAL4^ChAT.7.4 is a non-suppressor of adult brain phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^ChAT.7.4

(Ghosh and Feany, 2004)

Sirt1^EP2300, Scer\GAL4^ChAT.7.4 is a non-suppressor of adult brain phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^ChAT.7.4

(Ghosh and Feany, 2004)

tara^EP3463, Scer\GAL4^ChAT.7.4 is a non-suppressor of adult brain phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^ChAT.7.4

(Ghosh and Feany, 2004)

pum^EP3461, Scer\GAL4^ChAT.7.4 is a non-suppressor of adult brain phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^ChAT.7.4

(Ghosh and Feany, 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^ChAT.7.4/Adar^EA.3.4.UAS rescues Adar^5G1

(Deng et al., 2020)

Nf1^UAS.cdNa/Scer\GAL4^ChAT.7.4 rescues Nf1^P2/Nf1^P1

(Bai et al., 2018)

Tusp^UAS.cYa/Scer\GAL4^ChAT.7.4 rescues Df(3R)Tusp-D

(Yoon et al., 2017)

Smn^UAS.Tag:FLAG/Scer\GAL4^ChAT.7.4 rescues Smn^X7

(Imlach et al., 2012)

Scer\GAL4^ChAT.7.4, inc^f00285 rescues inc^f00285

(Pfeiffenberger and Allada, 2012)

ChAT^UAS.GFP/Scer\GAL4^ChAT.7.4 rescues ChAT^ts1

(Sadananda et al., 2012)

tutl^RE40452.UAS/Scer\GAL4^ChAT.7.4 rescues tutl²³

(Zhou et al., 2012)

Klp64D^UAS.GFP/Scer\GAL4^ChAT.7.4 rescues Klp64D^k1

(Jana et al., 2011)

Adar^3.4.S.UAS/Scer\GAL4^ChAT.7.4 rescues Adar^5G1

(Keegan et al., 2011)

Scer\GAL4^ChAT.7.4/Adar^3.4.UAS rescues Adar^5G1

(Keegan et al., 2011)

Scer\GAL4^ChAT.7.4/Adar^3.4.UAS rescues Adar^1F4

(Keegan et al., 2011)

Adar^3.4.S.UAS/Scer\GAL4^ChAT.7.4 rescues Adar^5G1

(Keegan et al., 2005)

sbb^UAS.cYa/Scer\GAL4^ChAT.7.4 rescues sbb⁰³⁴³²

(Suster et al., 2004)

Partially rescues

Scer\GAL4^ChAT.7.4/Adar^3.4.UAS partially rescues Adar^5G1

(Deng et al., 2020)

Scer\GAL4^ChAT.7.4/Adar^3.4.UAS partially rescues Adar^E374A

(Deng et al., 2020)

ChT^UAS.cHa/Scer\GAL4^ChAT.7.4 partially rescues ChT^KK109385

(Hamid et al., 2019)

Scer\GAL4^ChAT.7.4, inc^f00285 partially rescues inc^f00285

(Stavropoulos and Young, 2011)

kcc^UAS.cHb/Scer\GAL4^ChAT.7.4 partially rescues kcc^DHS1

(Hekmat-Scafe et al., 2010)

qvr^UAS.cWa/Scer\GAL4^ChAT.7.4 partially rescues qvr^EY04063

(Wu et al., 2010)

fbl^L.UAS/Scer\GAL4^ChAT.7.4 partially rescues fbl¹

(Wu et al., 2009)

Fails to rescue

Scer\GAL4^ChAT.7.4/Adar^EA.3.4.UAS fails to rescue Adar^5G1

(Keegan et al., 2011)

Scer\GAL4^ChAT.7.4/Oamb^UAS.K3 fails to rescue Oamb^unspecified

(Lee et al., 2009)

Oamb^UAS.AS/Scer\GAL4^ChAT.7.4 fails to rescue Oamb^unspecified

(Lee et al., 2009)

Dys^Dp186.UAS/Scer\GAL4^ChAT.7.4 fails to rescue Dys^Dp186.166.3

(Fradkin et al., 2008)

Scer\GAL4^ChAT.7.4/Ns3^UAS.Venus fails to rescue Ns3^G0431

(Kaplan et al., 2008)

Tbh^UAS.cMa/Scer\GAL4^ChAT.7.4 fails to rescue Tbh^nM18

(Zhou et al., 2008)

tok^{UAS.cSa.Tag:HA}/Scer\GAL4^ChAT.7.4 fails to rescue tok^K788/tok^D427

(Meyer and Aberle, 2006)

Nf1^UAS.cWa/Scer\GAL4^ChAT.7.4 fails to rescue Nf1^unspecified

(Walker et al., 2006)

Scer\GAL4^ChAT.7.4/Itpr^UAS.cVa fails to rescue Itpr^wc703/Itpr^ug3

(Banerjee et al., 2004)

Scer\GAL4^ChAT.7.4/Itpr^UAS.cVa fails to rescue Itpr^ka901/Itpr^ug3

(Joshi et al., 2004)

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

6793

w^*; P{ChAT-GAL4.7.4}19B P{UAS-GFP.S65T}Myo31DF^T2

56501

w^*; P{ET-GAL80}Gaz, P{ChAT-GAL4.7.4}19B P{UAS-GFP.S65T}Myo31DF^T2/CyO

56500

w^*; P{ChAT-GAL4.7.4}19B/CyO; PBac{20XUAS-IVS-GCaMP5G}VK00005/TM3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Chat^Gal4

(Demilly et al., 2011)

Gal4^cha19b

(Jana et al., 2011)

Gal4^chat19b

(Jana et al., 2011)

Scer\GAL4^ChAT.7.4

Scer\GAL4^Cha.7.4

Scer\GAL4^Cha.PW

Name Synonyms

Secondary FlyBase IDs

References (219)

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