Expression of Pka-R1BDK.Scer\UAS.T:Ivir\HA1 under the control of either Scer\GAL4Tab2-201Y, Scer\GAL4c107 or Scer\GAL4c522 leads to a decrease in ethanol sensitivity. The levels of absorbed ethanol is not affected in these flies. Decreased ethanol sensitivity of Scer\GAL4Tab2-201Y>Pka-R1BDK.Scer\UAS.T:Ivir\HA1 flies remains after mushroom body ablation. Flies expressing Pka-R1BDK.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Tab2-201Y, and to a lesser extent when under the control of Scer\GAL4c107, are resistant to the locomotor-depressant effects of ethanol. In contrast, when expression of Pka-R1BDK.Scer\UAS.T:Ivir\HA1 is driven by Scer\GAL4c522 or Scer\GAL4c290, ethanol-induced locomotor effects are not significantly different from wild type. Expression of Pka-R1BDK.Scer\UAS.T:Ivir\HA1 under the control of both Scer\GAL4Tab2-201Y and Scer\GAL4c747 supresses the ethanol resistance seen when only Scer\GAL4Tab2-201Y is used as a driver. Expression of Pka-R1BDK.Scer\UAS.T:Ivir\HA1 driven by the following lines does not affect sensitivity of flies to ethanol: Scer\GAL4c309, Scer\GAL4c747, Scer\GAL4c739, Scer\GAL4c758, Scer\GAL4MJ162A, Scer\GAL4C-765, Scer\GAL4c346, Scer\GAL4064Y, Scer\GAL4c767, Scer\GAL4C41, Scer\GAL4c119, Scer\GAL4c491, Scer\GAL4c453, Scer\GAL4104Y, Scer\GAL4OK348, Scer\GAL4c687, Scer\GAL4007Y, Scer\GAL4078Y, Scer\GAL4Mz423, Scer\GAL4c229, Scer\GAL4Okt30, Scer\GAL4Feb204, Scer\GAL4Kurs58, Scer\GAL4Jan129, Scer\GAL4Mai301, Scer\GAL4Jan229, Scer\GAL4Kurs21, Scer\GAL4Mai179, Scer\GAL4Kurs6, Scer\GAL4OK086, Scer\GAL4OK028, Scer\GAL4OK062, Scer\GAL4OK144D, Scer\GAL4OK144L, Scer\GAL4OK165, Scer\GAL4OK168, Scer\GAL4OK175, Scer\GAL4OK309, Scer\GAL4elav.PLu, Scer\GAL4c123a, Scer\GAL4Mhc.PW, Scer\GAL4c290, Scer\GAL4121Y, Scer\GAL417d, Scer\GAL4c302, Scer\GAL4c584, Scer\GAL4c819, Scer\GAL4c81, Scer\GAL4c105, Scer\GAL4c161, Scer\GAL4c217, Scer\GAL4c338, Scer\GAL4c401a, Scer\GAL4c481, Scer\GAL4c628, Scer\GAL4Feb170, Scer\GAL4c159b, Scer\GAL4c259, Scer\GAL4c061, Scer\GAL4Aph-4-c232, Scer\GAL4c561, and Scer\GAL4c548. Heat shock-induced expression of Pka-R1BDK.Scer\UAS.T:Ivir\HA1, driven by Scer\GAL4hs.PB, results in increased sensitivity to ethanol.