FlyBase Allele Report: Dmel\Pka-C1[K75A.UAS]

General Information

Symbol

Dmel\Pka-C1^K75A.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0126507

Feature type

allele

Associated gene

Dmel\Pka-C1

Associated Insertion(s)

Carried in Construct

P{UAS-Pka-C1.K75A}

Key Links

Transgenic product class

missense_variant

(Kiger and O'Shea, 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Kiger and O'Shea, 2001)

Mutagen

in vitro construct

(Kiger and O'Shea, 2001)

Progenitor genotype

Carried in construct

P{UAS-Pka-C1.K75A}

Cytology

Description

UAS regulatory sequences drive expression of mutated Pka-C1 (carries the amino acid replacement K75A).

(Kiger and O'Shea, 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Pka-C1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^ppk.PG

(Copf, 2014)

fertile, with Scer\GAL4^30A

(Kiger and O'Shea, 2001)

lethal, with Scer\GAL4^PP3

(Kiger and O'Shea, 2001)

lethal - all die before end of pupal stage, with Scer\GAL4^JW1

(Kiger and O'Shea, 2001)

some die during pupal stage, with Scer\GAL4^JW1

(Kiger and O'Shea, 2001)

viable, with Scer\GAL4^30A

(Kiger and O'Shea, 2001)

viable, with Scer\GAL4^KO5

(Kiger and O'Shea, 2001)

viable, with Scer\GAL4^RK5

(Kiger and O'Shea, 2001)

visible, with Scer\GAL4^30A

(Kiger and O'Shea, 2001)

visible, with Scer\GAL4^RZ4

(Kiger and O'Shea, 2001)

wild-type, with Scer\GAL4^RK5

(Kiger and O'Shea, 2001)

Phenotype Manifest In

adult thorax, with Scer\GAL4^JW1

(Kiger and O'Shea, 2001)

dendrite, with Scer\GAL4^ppk.PG

(Copf, 2014)

dendritic branch point, with Scer\GAL4^ppk.PG

(Copf, 2014)

Golgi apparatus, with Scer\GAL4^ppk.PG

(Copf, 2014)

larval multidendritic class IV neuron, with Scer\GAL4^ppk.PG

(Copf, 2014)

mesonotum | ectopic, with Scer\GAL4^JW1

(Kiger and O'Shea, 2001)

wing, with Scer\GAL4^30A

(Kiger and O'Shea, 2001)

wing, with Scer\GAL4^JW1

(Kiger and O'Shea, 2001)

wing, with Scer\GAL4^PP3

(Kiger and O'Shea, 2001)

wing | anterior, with Scer\GAL4^KO5

(Kiger and O'Shea, 2001)

wing | anterior, with Scer\GAL4^PP3

(Kiger and O'Shea, 2001)

wing | anterior, with Scer\GAL4^RZ4

(Kiger and O'Shea, 2001)

wing vein | ectopic, with Scer\GAL4^RZ4

(Kiger and O'Shea, 2001)

Detailed Description

Statement

Reference

The class IV dendritic arborization neurons in flies expressing Pka-C1^{K75A.Scer\UAS} under the control of Scer\GAL4^ppk.PG show a significant decrease in the complexity of dendritic arbors in respect to the total dendritic length. The total number of dendritic branching points is also decreased. There are significantly fewer golgi outposts in the distal arbor compartments compared to wild type. The distribution of early endosomes is altered compared to wild type and the number of endosomes in the distal compartments is significantly lower.

(Copf, 2014)

Expression of Pka-C1^{K75A.Scer\UAS} under the control of Scer\GAL4^JW1 results in pupal lethality in most lines tested. In the P{UAS-Pka-C1.K75A}10.4 line, many adults cannot free themselves from the pupal case. Of those that do, a few have wings with large anterior duplications, others have large wing masses enclosed in the thorax and the remainder have duplications of the notum in place of the wings. Expression of Pka-C1^{K75A.Scer\UAS} under the control of Scer\GAL4^KO5 generally results in normal adults, although some have small wing outgrowths from the medial costal region of the anterior wing. Expression of Pka-C1^{K75A.Scer\UAS} under the control of Scer\GAL4^PP3 generally results in death at the pupal or pharate adult stage, except in the P{UAS-Pka-C1.K75A}10.4 line where adults are produced with spread wings and small outgrowths in the proximal costal region of the anterior wing. Expression of Pka-C1^{K75A.Scer\UAS} under the control of Scer\GAL4^RZ4 has variable effects on viability. Surviving adults have large anterior expansions of the wing, with a mass of vein material replacing wing blade in most of the duplicated region. Expression of Pka-C1^{K75A.Scer\UAS} under the control of Scer\GAL4^30A results in a small mirror-image duplication in some wings.