Open Close
General Information
Symbol
Dmel\PhmUAS.cTA
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Taghert
FlyBase ID
FBal0126508
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

An EcoRI/KpnI fragment of the Phm cDNA is driven by UASt sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

When PhmScer\UAS.cTA is driven by Scer\GAL4tim.PE or Scer\GAL4dimm-929 in a wild-type background, no degradation of behavioural rhythmicity is seen.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Phmk07623/PhmP(29) flies, rescued by PhmScer\UAS.cTA (driven by either Scer\GAL4CG11033-36Y or Scer\GAL4dimm-929) display two peaks of locomotor activity under cycling conditions. They have a normal peak time, locomotor levels accompanying these maxima are sustained, but do not anticipate the morning lights on transition. Phmk07623/PhmP(29) flies, rescued by PhmScer\UAS.cTA (driven by Scer\GAL4386Y) have wild-type locomotor activity under cycling conditions. Phmk07623/PhmP(29) flies, rescued by PhmScer\UAS.cTA (driven by Scer\GAL4CG11033-36Y) under constant conditions (DD) are very weakly rhythmic over days 1-2 and 3-9. 6% of these individuals are rhythmic. and they show a very low signal to noise ration (SNR) (about 0.3). PhmScer\UAS.cTA is driven by Scer\GAL4dimm-929 rescued flies are also poorly rhythmic during DD days 1-2. From days 3-9 these flies are only 3% rhythmic but have a SNR of about 0.8. When PhmScer\UAS.cTA is driven by Scer\GAL4386Y flies activity approaches that of wild-type. PhmP(29) flies, rescued by PhmScer\UAS.cTA (driven by Scer\GAL4dimm-929) display a low percentage rhythmic animals but display a moderately high SNR. Phmk07623 flies, rescued by PhmScer\UAS.cTA (driven by Scer\GAL4dimm-929) have a lower average SNR, but 77% are rhythmic. The addition of Scer\GAL4Appl.G1a or Scer\GAL4P2.4.Pdf to Scer\GAL4Appl.G1a flies that are rescued by Scer\GAL4dimm-929 driven by Scer\GAL4dimm-929 increases the percentage of rhythmic individuals but does not improve the SNR.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
PhmScer\UAS.cTA
PhmUAS.cTA
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Taghert
Secondary FlyBase IDs
    References (1)