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General Information
Symbol
Dmel\E2f276Q1
Species
D. melanogaster
Name
FlyBase ID
FBal0126597
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
de2f276Q1, E2f276Q.1
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
deletion
Comment:

Approximate endpoints of excision deletion reported as extending from a position 378bp upstream of the E2f2 transcription start to amino acid 5 of E2f2.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

A deletion extends from 378 bp upstream of the E2f2 transcription start site to amino acid 5 of the coding sequence.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

salivary gland & nucleus (with E2f2p111-5)

Detailed Description
Statement
Reference

E2f276Q1/E2f2329 Mpp6+t4 animals have a pattern of irradiation induced apoptosis in the late third instar wing disc which is identical to that seen in wild-type animals. (Mpp6+t4 is present to rescued loss of Mpp6 in Df(2L)E2f2329.

20% to 35% of homozygotes survive to adulthood. These flies have reduced fertility. Only 50% of eggs laid by homozygous mothers hatch, and about 5% of eggs laid by homozygous females have fragile, thin chorions.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement
Reference

E2f2[+]/E2f276Q1 is a suppressor of visible | dominant phenotype of gcmPyx

Other
Phenotype Manifest In
Enhanced by
Statement
Reference

E2f2p111-5/E2f276Q1 has salivary gland & nucleus phenotype, enhanceable by E2f1su89

Suppressed by
Statement
Reference

E2f2p111-5/E2f276Q1, E2f1su89 has macrochaeta & mesothoracic tergum phenotype, suppressible by CycEAR95

E2f2p111-5/E2f276Q1, E2f1su89 has salivary gland & G1 phase phenotype, suppressible by CycEAR95

E2f2p111-5/E2f276Q1, E2f1su89 has salivary gland & nucleus phenotype, suppressible by CycEAR95

Suppressor of
Statement
Reference

E2f2[+]/E2f276Q1 is a suppressor of chaeta | supernumerary phenotype of gcmPyx

Other
Statement
Reference

E2f2p111-5/E2f276Q1, E2f1su89 has cell cycle & scutellum phenotype

E2f2p111-5/E2f276Q1, E2f1su89 has macrochaeta & mesothoracic tergum phenotype

E2f2p111-5/E2f276Q1, E2f1su89 has salivary gland & G1 phase phenotype

E2f2p111-5/E2f276Q1, E2f1su89 has salivary gland & nucleus phenotype

Additional Comments
Genetic Interactions
Statement
Reference

The addition of E2f276Q1/E2f2p111-5 to homozygous E2fsu89 leads to lethality in the majority of mutants - 75% of the mutants die during the larvae stage, 24% during the pupal stage, with only 1% surviving to adulthood. Larval development is asynchronous: while most larvae are smaller, some healthy larvae can reach sizes similar to wild-type, and their development is only delayed about a day. The surviving adults do not show gross defects in their adult structures, though 88% display extremely short macrochaetae on the notum. These macrochaetae form the proper structure but fail to grow to its normal size. This seems to be a result of a defect in endoreplication in those cells. Mutants also exhibit defects in maintaining cell cycle arrest in the scutella. Third instar larval salivary glands are also smaller in these animals. The number of cells is wild-type, but the DNA content of those cells is about 40% of wild-type, probably due to fewer rounds of endocycles. The length of each endocycle is correspondingly longer due to an increased length of the gap phase. The addition of CycEAR95 to E2f276Q1/E2f2p111-5, E2fsu89, partially suppresses the salivary gland endoreplication defect. Salivary gland nuclei in these animals have about 70% of the DNA content of wild-type. The lethality is also partially suppressed - 52% of animals survive to the pupal stage (compared to 25%). Finally the macrochaetae phenotype is strongly suppressed. - 99% of flies show normal macrochaetae (as opposed to 12%).

The addition of E2f276Q1/Df(2L)G5.1 to E2f91/E2frM729 flies almost completely suppresses the E2f91/E2frM729 phenotypes - mutants develop normally without any significant delay in larval growth, reach pupal stage and finally die as mid- or late pupae. The pattern of DNA synthesis in eye discs is largely normal. The pattern of DNA synthesis in eye discs is largely normal.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer

This allele was made by a duplication event caused by the excision of P{lacW}Mpp6k16403 that replaces the 5' UTR sequences removed in Df(2L)Q3.1.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (9)