Allele Dmel\Pvr^DN.Scer\UAS

General Information
Symbol	Dmel\Pvr^DN.Scer\UAS	Species	D. melanogaster
Name		FlyBase ID	FBal0127282
Feature type	allele	Associated gene	Dmel\Pvr
Also Known As	PVR^DN

Allele class	antimorphic allele - genetic evidence
Mutagen	in vitro construct - deletion, in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	antimorphic allele - genetic evidence (Olofsson and Page, 2005)
Mutagen	in vitro construct - deletion (Duchek et al., 2001) in vitro construct - regulatory fusion (Duchek et al., 2001)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Contains only the extracellular and transmembrane domains of Pvr, driven by Scer\UAS sequences. (Duchek et al., 2001)
Carried in construct	P{UAS-Pvr.DN} (Bianco et al., 2007, Sears et al., 2003, Duchek et al., 2001, Fulga and Rorth, 2002, Geisbrecht and Montell, 2004, Macias et al., 2004, Olofsson and Page, 2005, Zettervall et al., 2004, McDonald et al., 2006, Mathieu et al., 2007, Inaki et al., 2012, Wang et al., 2010, Wang et al., 2010, Bunt et al., 2010, Kuranaga et al., 2011, Ohsawa et al., 2011, Wang et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective (Sears et al., 2003)
Phenotype Manifest In
	adult external abdomen \| dorsal, with Scer\GAL4^dpp.blk1 (Macias et al., 2004) adult external abdomen \| dorsal, with Scer\GAL4^en-e16E (Macias et al., 2004) border follicle cell, with Scer\GAL4^slbo.2.6 (Geisbrecht and Montell, 2004, Duchek et al., 2001, McDonald et al., 2006, Mathieu et al., 2007, Wang et al., 2006) border follicle cell & filopodium, with Scer\GAL4^slbo.2.6 (Fulga and Rorth, 2002) embryonic/larval glial cell (Sears et al., 2003) embryonic/larval hemocyte (Sears et al., 2003) embryonic anterior Malpighian tubule, with Scer\GAL4^gcm-rA87.P (Bunt et al., 2010) embryonic hemocyte, with Scer\GAL4^s.gcm (Olofsson and Page, 2005) hemocyte, with Scer\GAL4^gcm-rA87.P (Bunt et al., 2010) hemocyte, with Scer\GAL4^He.PZ (Zettervall et al., 2004) male genitalia, with Scer\GAL4^en.PU (Kuranaga et al., 2011) male terminalia, with Scer\GAL4^dpp.blk1 (Macias et al., 2004) male terminalia, with Scer\GAL4^en-e16E (Macias et al., 2004) presumptive embryonic/larval central nervous system (Sears et al., 2003) ventral nerve cord \| embryonic stage, with Scer\GAL4^s.gcm (Olofsson and Page, 2005) ventral nerve cord \| embryonic stage, with Scer\GAL4^sim.PS (Olofsson and Page, 2005)
Detailed Description
	Statement Reference Males expressing Pvr[DN.Scer\UAS] under the control of Scer\GAL4[en.PU] display mis-oriented adult genitalia, and the acceleration of genitalia rotation is significantly impaired in these flies compared to controls. (Kuranaga et al., 2011) Expression of Pvr[DN.Scer\UAS] under the control of Scer\GAL4[gcm-rA87.P] stalls haemocyte migration in the embryo, and anterior Malpighian tubule positioning is disrupted. The anterior pair of tubules project into the poster half of the embryo and do not take up their anterior position as in wild-type. (Bunt et al., 2010) Border follicle cells that are expressing Pvr^DN.Scer\UAS under the control of Scer\GAL4^slbo.2.6 show mild defects in posterior migration towards the oocyte. (Mathieu et al., 2007) Expression of Pvr^DN.Scer\UAS under the control of Scer\GAL4^slbo.2.6 causes border cell misguidance defects but the penetrance of this phenotype is relatively low; only 16% of egg chambers are affected. (McDonald et al., 2006) Expression of Pvr[DN.Scer\UAS] under the control of Scer\GAL4[slbo.2.6] results in incomplete border cell migration in 25% of stage 10 egg chambers. (Wang et al., 2006) Expression of Pvr[DN.Scer\UAS] in early hemocytes, driven by Scer\GAL4[s.gcm], disrupts the normal migration of hemocytes during embryonic development. In severe cases, the hemocytes become aggregated in the anterior part of the embryo by stage 15. At stage 17, the VNC is shorter in Pvr[DN.Scer\UAS] embryos than in wild-type, indicating that the VNC fails to condense correctly in Pvr[DN.Scer\UAS] mutants. Expression of Pvr[DN.Scer\UAS] in the lateral glia and not in hemocytes, driven by Scer\GAL4[158], does not cause either the hemocyte migration or VNC condensation phenotype. When Pvr[DN.Scer\UAS] is expressed in the mesodermal midline, driven by Scer\GAL4[sim.PS], VNC condensation is partially inhibited. (Olofsson and Page, 2005) Expression of Pvr^DN.Scer\UAS under the control of Scer\GAL4^slbo.2.6 results in a mild border follicle cell migration defect. (Geisbrecht and Montell, 2004) When expression is driven by Scer\GAL4^dpp.blk1 or Scer\GAL4^en-e16E the male terminalia are rotated. The effect is more pronounced with Scer\GAL4^en-e16E than with Scer\GAL4^dpp.blk1, though in both cases shows variable penetrance and expressivity. (Macias et al., 2004) When expression is driven by Scer\GAL4^He.PZ, hemocyte proliferation is decreased and lamellocyte and crystal cells numbers are unaffected. (Zettervall et al., 2004) Expression of Pvr^DN.Scer\UAS under the control of Scer\GAL4^sim.P3.7 does not result in a detectable mutant central nervous system phenotype in embryos. Hemocytes are largely clustered near the dorsal and anterior regions of the embryo in stage 16 embryos expressing Pvr^DN.Scer\UAS under the control of Scer\GAL4^gcm.PP, in contrast to wild-type embryos where they are dispersed throughout the embryo. These embryos also show rounding of the central nervous system axon commissures and mispositioning of repo-positive glial cells. (Sears et al., 2003) Border cells in the ovaries of Pvr^DN.Scer\UAS; Scer\GAL4^slbo.2.6 animals form dramatically fewer long cellular extensions (20-40 μm) and significantly fewer intermediate cellular extensions (10-20 μm). (Fulga and Rorth, 2002) When expressed under the control of Scer\GAL4^slbo.2.6 some delay in posterior migration of border cells is seen. Less than 60% of border cell clusters reach the oocyte at stage 10. (Duchek et al., 2001)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement Reference Egfr^DN.Scer\UAS, Pvr^DN.Scer\UAS, Scer\GAL4^slbo.2.6 has border follicle cell phenotype, enhanceable by Tie^DN.Scer\UAS, Scer\GAL4^slbo.2.6 (Wang et al., 2006) Pvr^DN.Scer\UAS, Scer\GAL4^dpp.blk1 has adult external abdomen \| dorsal phenotype, enhanceable by Pvf1[-] (Macias et al., 2004) Pvr^DN.Scer\UAS, Scer\GAL4^dpp.blk1 has male terminalia phenotype, enhanceable by Pvf1[-] (Macias et al., 2004) Pvr^DN.Scer\UAS, Scer\GAL4^en-e16E has adult external abdomen \| dorsal phenotype, enhanceable by Pvf1[-] (Macias et al., 2004) Pvr^DN.Scer\UAS, Scer\GAL4^en-e16E has male terminalia phenotype, enhanceable by Pvf1[-] (Macias et al., 2004) Pvr^DN.Scer\UAS, Scer\GAL4^slbo.2.6 has border follicle cell & filopodium phenotype, enhanceable by Egfr^DN.Scer\UAS, Scer\GAL4^slbo.2.6 (Fulga and Rorth, 2002) Pvr^DN.Scer\UAS, Scer\GAL4^slbo.2.6 has border follicle cell & filopodium phenotype, enhanceable by Pvf1^EPg11235, Scer\GAL4^slbo.2.6 (Fulga and Rorth, 2002) Pvr^DN.Scer\UAS, Scer\GAL4^slbo.2.6 has border follicle cell phenotype, enhanceable by bsk²/bsk² (Mathieu et al., 2007) Pvr^DN.Scer\UAS, Scer\GAL4^slbo.2.6 has border follicle cell phenotype, enhanceable by Egfr^DN.Scer\UAS, Scer\GAL4^slbo.2.6 (Duchek et al., 2001, McDonald et al., 2006) Pvr^DN.Scer\UAS, Scer\GAL4^slbo.2.6 has border follicle cell phenotype, enhanceable by hep^Act.Scer\UAS, Scer\GAL4^slbo.2.6 (Mathieu et al., 2007) Pvr^DN.Scer\UAS, Scer\GAL4^slbo.2.6 has border follicle cell phenotype, enhanceable by puc^PBac3929/puc^PBac3929 (Mathieu et al., 2007) Pvr^DN.Scer\UAS, Scer\GAL4^slbo.2.6 has border follicle cell phenotype, enhanceable by Tie^DN.Scer\UAS, Scer\GAL4^slbo.2.6 (Wang et al., 2006) Pvr^DN.Scer\UAS, Scer\GAL4^slbo.2.6 has border follicle cell phenotype, enhanceable by vn^EPg35521, Scer\GAL4^slbo.2.6 (Duchek et al., 2001)
Suppressed by
Statement Reference Pvr^DN.Scer\UAS, Scer\GAL4^slbo.2.6, hep^Act.Scer\UAS has border follicle cell phenotype, suppressible \| partially by kay^{bZip.Scer\UAS}, Scer\GAL4^slbo.2.6 (Mathieu et al., 2007) Pvr^DN.Scer\UAS, Scer\GAL4^slbo.2.6, hep^Act.Scer\UAS has border follicle cell phenotype, suppressible \| partially by kay^{dsRNA.Scer\UAS}, Scer\GAL4^slbo.2.6 (Mathieu et al., 2007) Pvr^DN.Scer\UAS, Scer\GAL4^slbo.2.6, hep^Act.Scer\UAS has border follicle cell phenotype, suppressible \| partially by th^Scer\UAS.cHa, Scer\GAL4^slbo.2.6 (Mathieu et al., 2007) Pvr^DN.Scer\UAS, Scer\GAL4^slbo.2.6 has border follicle cell phenotype, suppressible \| partially by th^{Scer\UAS.T:Ivir\HA1}, Scer\GAL4^slbo.2.6 (Geisbrecht and Montell, 2004)
NOT suppressed by
Statement Reference Pvr^DN.Scer\UAS, Scer\GAL4^slbo.2.6, hep^Act.Scer\UAS has border follicle cell phenotype, non-suppressible by BacA\p35^Scer\UAS.cHa, Scer\GAL4^slbo.2.6 (Mathieu et al., 2007) Pvr^DN.Scer\UAS, Scer\GAL4^slbo.2.6 has border follicle cell phenotype, non-suppressible by Mmus\Cd8a^{Scer\UAS.T:Avic\GFP}, Scer\GAL4^slbo.2.6 (Geisbrecht and Montell, 2004)
Enhancer of
Statement Reference Pvr^DN.Scer\UAS, Scer\GAL4^slbo.2.6 is an enhancer of border follicle cell & filopodium phenotype of Egfr^DN.Scer\UAS, Scer\GAL4^slbo.2.6 (Fulga and Rorth, 2002) Pvr^DN.Scer\UAS, Scer\GAL4^slbo.2.6 is an enhancer of border follicle cell & filopodium phenotype of Pvf1^EPg11235, Scer\GAL4^slbo.2.6 (Fulga and Rorth, 2002) Pvr^DN.Scer\UAS, Scer\GAL4^slbo.2.6 is an enhancer of border follicle cell phenotype of Egfr^DN.Scer\UAS, Scer\GAL4^slbo.2.6 (Duchek et al., 2001)
Other
Statement Reference Egfr^DN.Scer\UAS, Pvr^DN.Scer\UAS, Scer\GAL4^slbo.2.6 has border follicle cell phenotype (Inaki et al., 2012, Wang et al., 2006) Pvr^DN.Scer\UAS, Scer\GAL4^slbo.2.6, Shc^111-40 has border follicle cell \| somatic clone phenotype (Bianco et al., 2007)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of Pvr[DN.Scer\UAS] and Egfr[DN.Scer\UAS] under the control of Scer\GAL4[slbo.2.6] results in defects in border cell migration. (Inaki et al., 2012) Border cells clusters that are mutant for Shc^111-40 and also expressing Pvr^DN.Scer\UAS under the control of Scer\GAL4^slbo.2.6 show severely impaired posterior migration. (Bianco et al., 2007) Homozygous puc^PBac3929 border follicle cell clones that are also expressing Pvr^DN.Scer\UAS under the control of Scer\GAL4^slbo.2.6 show defects in posterior migration towards the oocyte that are more severe than the migration defects seen in border follicle cells expressing Pvr^DN.Scer\UAS under the control of Scer\GAL4^slbo.2.6 in an otherwise wild-type background. Co-expression of hep^Act.Scer\UAS enhances the severity of the posterior migration defects seen in border follicle cells that are expressing Pvr^DN.Scer\UAS under the control of Scer\GAL4^slbo.2.6. Homozygous bsk² border follicle cell clones that are also expressing Pvr^DN.Scer\UAS under the control of Scer\GAL4^slbo.2.6 show defects in migration that are more severe than border follicle cells expressing Pvr^DN.Scer\UAS under the control of Scer\GAL4^slbo.2.6 in an otherwise wild-type background. The border follicle cell migration defects that are seen in flies co-expressing both Pvr^DN.Scer\UAS and hep^Act.Scer\UAS under the control of Scer\GAL4^slbo.2.6 are partially suppressed by the co-expression of either kay^{dsRNA.Scer\UAS} or kay^{bZip.Scer\UAS}. The border follicle cell migration defects that are seen in flies co-expressing both Pvr^DN.Scer\UAS and hep^Act.Scer\UAS under the control of Scer\GAL4^slbo.2.6 are not strongly affected by the co-expression of either Jra^{dsRNA.cBa.Scer\UAS} or Jra^Jbz.Scer\UAS. The border follicle cell migration defects that are seen in flies co-expressing both Pvr^DN.Scer\UAS and hep^Act.Scer\UAS under the control of Scer\GAL4^slbo.2.6 are partially suppressed by the co-expression of th^Scer\UAS.cHa. (Mathieu et al., 2007) Coexpression of Egfr^DN.Scer\UAS and Pvr^DN.Scer\UAS, under the control of Scer\GAL4^slbo.2.6, results in a strong enhancement of the border cell misguidance phenotype seen when Pvr^DN.Scer\UAS is expressed alone; the penetrance of the phenotype is increased from 16 to 90%. (McDonald et al., 2006) Co-expression of Tie[DN.Scer\UAS] enhances the border cell migration defects caused by expression of Pvr[DN.Scer\UAS] under the control of Scer\GAL4[slbo.2.6], such that 37-58% of stage 10 egg chambers show border cell migration defects. Co-expression of Pvr[DN.Scer\UAS] and Egfr[DN.Scer\UAS] under the control of Scer\GAL4[c306] results in border cell migration defects. The severity of the migration defects is enhanced if the females also express Tie[DN.Scer\UAS]. (Wang et al., 2006) The border follicle cell migration defect seen in egg chambers expressing Pvr^DN.Scer\UAS under the control of Scer\GAL4^slbo.2.6 is suppressed by coexpression of th^{Scer\UAS.T:Ivir\HA1} (the frequency of migration defects is less than 10% in the rescued egg chambers). (Geisbrecht and Montell, 2004) Being mutant at Pvf1 enhances both the penetrance and expressivity of the Pvr^DN.Scer\UAS terminalia phenotype. (Macias et al., 2004) Border cells in the ovaries of Pvf1^EPg11235; Pvr^DN.Scer\UAS; Scer\GAL4^slbo.2.6 animals form lack long cellular extensions (20-40 μm) and form only very few intermediate cellular extensions (10-20 μm). Border cells in the ovaries of Egfr^DN.Scer\UAS; Pvr^DN.Scer\UAS; Scer\GAL4^slbo.2.6 animals form lack long cellular extensions (20-40 μm) and form only very few intermediate cellular extensions (10-20 μm). (Fulga and Rorth, 2002) Egfr^DN.Scer\UAS and Pvr^DN.Scer\UAS, when driven by Scer\GAL4^slbo.2.6, leads to a dramatic effect on border cell migration. 90% of border cell clusters migrate less than half way towards the oocyte. In 5% of egg chambers, border cell clusters are found off the direct track to the oocyte. The addition of vn^EPg35521 to Pvr^DN.Scer\UAS (driven by Scer\GAL4^slbo.2.6) leads to a strong enhancement of the border cell migration defect. Border cells do not reach the oocyte at stage 10. They usually migrate less than halfway, and are sometimes found off track. (Duchek et al., 2001)
Xenogenetic Interactions
Statement Reference The effect on border cell cluster migration when all border cells co-express Pvr[DN.Scer\UAS] and Egfr[DN.Scer\UAS] under the control of Scer\GAL4[slbo.2.6] and a single border cell expresses Egfr::Hsap\EGFR[hE-E.Scer\UAS.T:Avic\GFP] have been studied. In those border cell clusters where the single Egfr::Hsap\EGFR[hE-E.Scer\UAS.T:Avic\GFP]-expressing cell is located at the front of the cluster, the cluster on average moves forward, when the Egfr::Hsap\EGFR[hE-E.Scer\UAS.T:Avic\GFP]-expressing cell is in the back, the cluster on average moves relatively more backwards. (Inaki et al., 2012) In the absence of illumination, border cells expressing Egfr[DN.Scer\UAS] and Pvr[DN.Scer\UAS] under the control of Scer\GAL4[slbo.2.6] and carrying Hsap\RAC1[PA.Q61L.Scer\UAS.T:Disc\RFP-mCherry,T:Zzzz\PT1] fail to move forward. When Hsap\RAC1[PA.Q61L.Scer\UAS.T:Disc\RFP-mCherry,T:Zzzz\PT1] is photoactivated at the rear of the border cell cluster, rearward movement is seen. When photoactivation is stopped, the border cell cluster stops moving. Photo-inactivation of Hsap\RAC1[PA.T17N.Scer\UAS.T:Disc\RFP-mCherry,T:Zzzz\PT1] at the front of border cell clusters expressing Hsap\RAC1[PA.T17N.Scer\UAS.T:Disc\RFP-mCherry,T:Zzzz\PT1], Egfr[DN.Scer\UAS] and Pvr[DN.Scer\UAS] under the control of Scer\GAL4[slbo.2.6] results in local retraction. (Wang et al., 2010) The border follicle cell migration defects that are seen in flies co-expressing both Pvr^DN.Scer\UAS and hep^Act.Scer\UAS under the control of Scer\GAL4^slbo.2.6 are not suppressed by the co-expression of BacA\p35^Scer\UAS.cHa. (Mathieu et al., 2007) The border follicle cell migration defect seen in egg chambers expressing Pvr^DN.Scer\UAS under the control of Scer\GAL4^slbo.2.6 is not suppressed by coexpression of Mmus\Cd8a^{Scer\UAS.T:Avic\GFP}. (Geisbrecht and Montell, 2004)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Pvr^DN.Scer\UAS PVR^DN (Jang et al., 2009, Wang et al., 2010)
Name Synonym
Secondary FlyBase IDs

References ( 18 )
Research paper	Inaki et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109(6): 2027--2032 Effective guidance of collective migration based on differences in cell states. [FBrf0217439] Kuranaga et al., 2011, Development 138(8): 1493--1499 Apoptosis controls the speed of looping morphogenesis in Drosophila male terminalia. [FBrf0213320] Ohsawa et al., 2011, Dev. Cell 20(3): 315--328 Elimination of Oncogenic Neighbors by JNK-Mediated Engulfment in Drosophila. [FBrf0213219] Bunt et al., 2010, Dev. Cell 19(2): 296--306 Hemocyte-Secreted Type IV Collagen Enhances BMP Signaling to Guide Renal Tubule Morphogenesis in Drosophila. [FBrf0211514] Jang et al., 2009, Nat. Cell Biol. 11(5): 569--579 Border-cell migration requires integration of spatial and temporal signals by the BTB protein Abrupt. [FBrf0207765] Bianco et al., 2007, Nature 448(7151): 362--365 Two distinct modes of guidance signalling during collective migration of border cells. [FBrf0200493] Mathieu et al., 2007, Genetics 176(3): 1579--1590 A sensitized PiggyBac-based screen for regulators of border cell migration in Drosophila. [FBrf0201428] McDonald et al., 2006, Dev. Biol. 296(1): 94--103 Multiple EGFR ligands participate in guiding migrating border cells. [FBrf0193922] Wang et al., 2006, Dev. Cell 10(4): 483--495 Analysis of cell migration using whole-genome expression profiling of migratory cells in the Drosophila ovary. [FBrf0190227] Olofsson and Page, 2005, Dev. Biol. 279(1): 233--243 Condensation of the central nervous system in embryonic Drosophila is inhibited by blocking hemocyte migration or neural activity. [FBrf0183858] Geisbrecht and Montell, 2004, Cell 118(1): 111--125 A role for Drosophila IAP1-mediated caspase inhibition in Rac-dependent cell migration. [FBrf0179220] Macias et al., 2004, Int. J. Dev. Biol. 48(10): 1087--1094 PVF1/PVR signaling and apoptosis promotes the rotation and dorsal closure of the Drosophila male terminalia. [FBrf0184038] Zettervall et al., 2004, Proc. Natl. Acad. Sci. U.S.A. 101(39): 14192--14197 A directed screen for genes involved in Drosophila blood cell activation. [FBrf0180639] Sears et al., 2003, Development 130(15): 3557--3565 Macrophage-mediated corpse engulfment is required for normal Drosophila CNS morphogenesis. [FBrf0160936] Fulga and Rorth, 2002, Nat. Cell Biol. 4(9): 715--719 Invasive cell migration is initiated by guided growth of long cellular extensions. [FBrf0152247] Duchek et al., 2001, Cell 107(1): 17--26 Guidance of cell migration by the Drosophila PDGF/VEGF receptor. [FBrf0139607]
Supplementary material	Wang et al., 2010, Nat. Cell Biol. 12(6): Supplementary Information. [FBrf0211095] Wang et al., 2010, Nat. Cell Biol. 12(6): Online methods. [FBrf0211046]

Allele Dmel\PvrDN.Scer\UAS

Allele Dmel\Pvr^DN.Scer\UAS