border follicle cell & filopodium, with Scer\GAL4slbo.2.6
Expression of PvrDN.Scer\UAS under the control of Scer\GAL4slbo.2.6 alters the protrusion and retraction morphodynamics in border cell cluster migration.
Scer\GAL4He.PZ-mediated expression of PvrDN.Scer\UAS markedly downregulates hemocyte counts in larvae.
Expression of PvrCA.Scer\UAS specifically for 10 days 3-5 days after eclosion, under the control of Scer\GAL4esg-NP5130 and Scer\GAL80ts.αTub84B results in a striking reduction of esg>GFP positive cell clusters.
Inhibition of Pvr through Scer\GAL4esg-NP5130-led expression of PvrDN.Scer\UAS improves survival to P. entomophila infection.
Males expressing PvrDN.Scer\UAS under the control of Scer\GAL4en.PU display mis-oriented adult genitalia, and the acceleration of genitalia rotation is significantly impaired in these flies compared to controls.
Border follicle cell clusters in females expressing PvrDN.Scer\UAS under the control of Scer\GAL4slbo.2.6 show significantly reduced net cluster movement compared to wild type in both early and late phases of posterior migration. The cells show increased tumbling in the early phase of posterior migration. The number of cellular extensions from the front of the border cell cluster is reduced compared to wild type in the early phase of posterior migration, while the number of back and side cellular extensions is increased.
Expression of PvrDN.Scer\UAS under the control of Scer\GAL4gcm-rA87.P stalls haemocyte migration in the embryo, and anterior Malpighian tubule positioning is disrupted. The anterior pair of tubules project into the poster half of the embryo and do not take up their anterior position as in wild-type.
Border follicle cells that are expressing PvrDN.Scer\UAS under the control of Scer\GAL4slbo.2.6 show mild defects in posterior migration towards the oocyte.
Expression of PvrDN.Scer\UAS under the control of Scer\GAL4slbo.2.6 causes border cell misguidance defects but the penetrance of this phenotype is relatively low; only 16% of egg chambers are affected.
Expression of PvrDN.Scer\UAS under the control of Scer\GAL4slbo.2.6 results in incomplete border cell migration in 25% of stage 10 egg chambers.
Expression of PvrDN.Scer\UAS in early hemocytes, driven by Scer\GAL4s.gcm, disrupts the normal migration of hemocytes during embryonic development. In severe cases, the hemocytes become aggregated in the anterior part of the embryo by stage 15. At stage 17, the VNC is shorter in PvrDN.Scer\UAS embryos than in wild-type, indicating that the VNC fails to condense correctly in PvrDN.Scer\UAS mutants. Expression of PvrDN.Scer\UAS in the lateral glia and not in hemocytes, driven by Scer\GAL4158, does not cause either the hemocyte migration or VNC condensation phenotype. When PvrDN.Scer\UAS is expressed in the mesodermal midline, driven by Scer\GAL4sim.PS, VNC condensation is partially inhibited.
Expression of PvrDN.Scer\UAS under the control of Scer\GAL4slbo.2.6 results in a mild border follicle cell migration defect.
When expression is driven by Scer\GAL4dpp.blk1 or Scer\GAL4en-e16E the male terminalia are rotated. The effect is more pronounced with Scer\GAL4en-e16E than with Scer\GAL4dpp.blk1, though in both cases shows variable penetrance and expressivity.
When expression is driven by Scer\GAL4He.PZ, hemocyte proliferation is decreased and lamellocyte and crystal cells numbers are unaffected.
Expression of PvrDN.Scer\UAS under the control of Scer\GAL4sim.P3.7 does not result in a detectable mutant central nervous system phenotype in embryos. Hemocytes are largely clustered near the dorsal and anterior regions of the embryo in stage 16 embryos expressing PvrDN.Scer\UAS under the control of Scer\GAL4gcm.PP, in contrast to wild-type embryos where they are dispersed throughout the embryo. These embryos also show rounding of the central nervous system axon commissures and mispositioning of repo-positive glial cells.
Border cells in the ovaries of PvrDN.Scer\UAS; Scer\GAL4slbo.2.6 animals form dramatically fewer long cellular extensions (20-40 μm) and significantly fewer intermediate cellular extensions (10-20 μm).
When expressed under the control of Scer\GAL4slbo.2.6 some delay in posterior migration of border cells is seen. Less than 60% of border cell clusters reach the oocyte at stage 10.
PvrDN.UASp, Scer\GAL4NP5130 has abnormal immune response phenotype, suppressible by Scer\GAL80ts.αTub84B/hepAct.UAS, Scer\GAL4NP5130
AlkJF02668, PvrDN.UASp, Scer\GAL4phtm.PU has abnormal eclosion rhythm phenotype
EgfrDN.UAS, PvrDN.UASp, Scer\GAL4slbo.2.6, TieDN.UAS has abnormal cell migration phenotype
EgfrDN.UAS, PvrDN.UASp, Scer\GAL4slbo.2.6 has abnormal cell migration phenotype
EgfrDN.UAS, PvrDN.UASp, Rac1N17.UAS, Scer\GAL4slbo.2.6 has abnormal cell migration phenotype
PvrDN.UASp, Rac1N17.UAS, Scer\GAL4slbo.2.6 has abnormal cell migration phenotype
PvrDN.UASp, Scer\GAL4slbo.2.6 has border follicle cell phenotype, enhanceable by EgfrUAS.cBa, Scer\GAL4slbo.2.6
PvrDN.UASp, Scer\GAL4slbo.2.6 has border follicle cell phenotype, enhanceable by pucPBac3929/pucPBac3929
PvrDN.UASp, Scer\GAL4slbo.2.6 has border follicle cell phenotype, enhanceable by hepAct.UAS, Scer\GAL4slbo.2.6
PvrDN.UASp, Scer\GAL4slbo.2.6 has border follicle cell phenotype, enhanceable by bsk2/bsk2
PvrDN.UASp, Scer\GAL4slbo.2.6 has border follicle cell phenotype, enhanceable by EgfrDN.UAS, Scer\GAL4slbo.2.6
PvrDN.UASp, Scer\GAL4slbo.2.6 has border follicle cell phenotype, enhanceable by TieDN.UAS, Scer\GAL4slbo.2.6
EgfrDN.UAS, PvrDN.UASp, Scer\GAL4slbo.2.6 has border follicle cell phenotype, enhanceable by TieDN.UAS, Scer\GAL4slbo.2.6
PvrDN.UASp, Scer\GAL4en-e16E has adult external abdomen | dorsal phenotype, enhanceable by Pvf1[-]
PvrDN.UASp, Scer\GAL4en-e16E has male terminalia phenotype, enhanceable by Pvf1[-]
PvrDN.UASp, Scer\GAL4dpp.blk1 has adult external abdomen | dorsal phenotype, enhanceable by Pvf1[-]
PvrDN.UASp, Scer\GAL4dpp.blk1 has male terminalia phenotype, enhanceable by Pvf1[-]
PvrDN.UASp, Scer\GAL4slbo.2.6 has border follicle cell & filopodium phenotype, enhanceable by EgfrDN.UAS, Scer\GAL4slbo.2.6
PvrDN.UASp, Scer\GAL4slbo.2.6 has border follicle cell & filopodium phenotype, enhanceable by Pvf1EPg11235, Scer\GAL4slbo.2.6
PvrDN.UASp, Scer\GAL4slbo.2.6 has border follicle cell phenotype, enhanceable by vnEPg35521, Scer\GAL4slbo.2.6
PvrDN.UASp, Scer\GAL4NP5130 has adult midgut phenotype, suppressible by hepAct.UAS, Scer\GAL4NP5130
PvrDN.UASp, Scer\GAL4slbo.2.6, hepAct.UAS has border follicle cell phenotype, suppressible | partially by kayRNAi.UAS, Scer\GAL4slbo.2.6
PvrDN.UASp, Scer\GAL4slbo.2.6, hepAct.UAS has border follicle cell phenotype, suppressible | partially by kaybZip.UAS, Scer\GAL4slbo.2.6
PvrDN.UASp, Scer\GAL4slbo.2.6, hepAct.UAS has border follicle cell phenotype, suppressible | partially by Diap1UAS.cHa, Scer\GAL4slbo.2.6
PvrDN.UASp, Scer\GAL4slbo.2.6 has border follicle cell phenotype, suppressible | partially by Diap1UAS.Tag:HA, Scer\GAL4slbo.2.6
PvrDN.UASp, Scer\GAL4slbo.2.6, hepAct.UAS has border follicle cell phenotype, non-suppressible by BacA\p35UAS.cHa, Scer\GAL4slbo.2.6
PvrDN.UASp, Scer\GAL4slbo.2.6 has border follicle cell phenotype, non-suppressible by Mmus\Cd8aUAS.cLa.EGFP, Scer\GAL4slbo.2.6
PvrDN.UASp, Scer\GAL4slbo.2.6 is an enhancer of border follicle cell phenotype of EgfrUAS.cBa, Scer\GAL4slbo.2.6
PvrDN.UASp, Scer\GAL4slbo.2.6 is an enhancer of border follicle cell & filopodium phenotype of EgfrDN.UAS, Scer\GAL4slbo.2.6
PvrDN.UASp, Scer\GAL4slbo.2.6 is an enhancer of border follicle cell & filopodium phenotype of Pvf1EPg11235, Scer\GAL4slbo.2.6
PvrDN.UASp, Scer\GAL4slbo.2.6 is an enhancer of border follicle cell phenotype of EgfrDN.UAS, Scer\GAL4slbo.2.6
EgfrDN.UAS, PvrDN.UASp, Scer\GAL4slbo.2.6, TieDN.UAS has border follicle cell phenotype
EgfrDN.UAS, PvrDN.UASp, Rac1N17.UAS, Scer\GAL4slbo.2.6 has border follicle cell phenotype
PvrDN.UASp, Rac1N17.UAS, Scer\GAL4slbo.2.6 has border follicle cell phenotype
EgfrDN.UAS, PvrDN.UASp, Scer\GAL4slbo.2.6 has border follicle cell phenotype
PvrDN.UASp, Scer\GAL4slbo.2.6, Shc111-40 has border follicle cell | somatic clone phenotype
Flies expressing EgfrDN.Scer\UAS and PvrDN.Scer\UAS under the control of Scer\GAL4slbo.2.6 show differences in the migration behaviour of border cell clusters. First, a distinct front-back polarity is evident in wild-type clusters, which show spatially segregated protrusion and retraction with high protrusion velocities predominating at the front and high retraction velocities at the back. This spatial segregation is lost in clusters lacking guidance-receptor activities. Second, these double mutant clusters display overall slower protrusion and retraction velocities.
Flies expressing Rac1N17.Scer\UAS, PvrDN.Scer\UAS and EgfrDN.Scer\UAS exhibit a change in border cell migration, with changes in local protrusion and retraction behaviour that are different to those found in Rac1N17.Scer\UAS single mutants.
Flies expressing Rac1N17.Scer\UAS amd PvrDN.Scer\UAS exhibit a change in border cell migration, with changes in local protrusion and retraction behaviour that are different to those found in Rac1N17.Scer\UAS single mutants.
The addition of a dominant-negative TieDN.Scer\UAS to border cells expressing PvrDN.Scer\UAS and EgfrDN.Scer\UAS alters the protrusion and retraction morphodynamics of border cell cluster migration.
Co-expression of PvrDN.Scer\UAS and hepAct.Scer\UAS in 3-5 day old adult flies, under the control of Scer\GAL4esg-NP5130 and Scer\GAL80ts.αTub84B results in ISC proliferation.
Co-expression of PvrDN.Scer\UAS and EgfrDN.Scer\UAS under the control of Scer\GAL4slbo.2.6 results in defects in border cell migration.
Border cell clusters show severe delays in initiating posterior migration and show strongly reduced forward speed once migratory in females co-expressing PvrDN.Scer\UAS and EgfrDN.Scer\UAS under the control of Scer\GAL4slbo.2.6. The number of cellular extensions from the front of the border cell cluster is reduced compared to wild type and an increase in side and back extensions.
Border cells clusters that are mutant for Shc111-40 and also expressing PvrDN.Scer\UAS under the control of Scer\GAL4slbo.2.6 show severely impaired posterior migration.
Homozygous pucPBac3929 border follicle cell clones that are also expressing PvrDN.Scer\UAS under the control of Scer\GAL4slbo.2.6 show defects in posterior migration towards the oocyte that are more severe than the migration defects seen in border follicle cells expressing PvrDN.Scer\UAS under the control of Scer\GAL4slbo.2.6 in an otherwise wild-type background.
Co-expression of hepAct.Scer\UAS enhances the severity of the posterior migration defects seen in border follicle cells that are expressing PvrDN.Scer\UAS under the control of Scer\GAL4slbo.2.6.
Homozygous bsk2 border follicle cell clones that are also expressing PvrDN.Scer\UAS under the control of Scer\GAL4slbo.2.6 show defects in migration that are more severe than border follicle cells expressing PvrDN.Scer\UAS under the control of Scer\GAL4slbo.2.6 in an otherwise wild-type background.
The border follicle cell migration defects that are seen in flies co-expressing both PvrDN.Scer\UAS and hepAct.Scer\UAS under the control of Scer\GAL4slbo.2.6 are partially suppressed by the co-expression of either kaydsRNA.Scer\UAS or kaybZip.Scer\UAS.
The border follicle cell migration defects that are seen in flies co-expressing both PvrDN.Scer\UAS and hepAct.Scer\UAS under the control of Scer\GAL4slbo.2.6 are not strongly affected by the co-expression of either JradsRNA.cBa.Scer\UAS or JraJbz.Scer\UAS.
The border follicle cell migration defects that are seen in flies co-expressing both PvrDN.Scer\UAS and hepAct.Scer\UAS under the control of Scer\GAL4slbo.2.6 are partially suppressed by the co-expression of thScer\UAS.cHa.
Coexpression of EgfrDN.Scer\UAS and PvrDN.Scer\UAS, under the control of Scer\GAL4slbo.2.6, results in a strong enhancement of the border cell misguidance phenotype seen when PvrDN.Scer\UAS is expressed alone; the penetrance of the phenotype is increased from 16 to 90%.
Co-expression of TieDN.Scer\UAS enhances the border cell migration defects caused by expression of PvrDN.Scer\UAS under the control of Scer\GAL4slbo.2.6, such that 37-58% of stage 10 egg chambers show border cell migration defects.
Co-expression of PvrDN.Scer\UAS and EgfrDN.Scer\UAS under the control of Scer\GAL4c306 results in border cell migration defects. The severity of the migration defects is enhanced if the females also express TieDN.Scer\UAS.
The border follicle cell migration defect seen in egg chambers expressing PvrDN.Scer\UAS under the control of Scer\GAL4slbo.2.6 is suppressed by coexpression of thScer\UAS.T:Ivir\HA1 (the frequency of migration defects is less than 10% in the rescued egg chambers).
Being mutant at Pvf1 enhances both the penetrance and expressivity of the PvrDN.Scer\UAS terminalia phenotype.
Border cells in the ovaries of Pvf1EPg11235; PvrDN.Scer\UAS; Scer\GAL4slbo.2.6 animals form lack long cellular extensions (20-40 μm) and form only very few intermediate cellular extensions (10-20 μm). Border cells in the ovaries of EgfrDN.Scer\UAS; PvrDN.Scer\UAS; Scer\GAL4slbo.2.6 animals form lack long cellular extensions (20-40 μm) and form only very few intermediate cellular extensions (10-20 μm).
EgfrDN.Scer\UAS and PvrDN.Scer\UAS, when driven by Scer\GAL4slbo.2.6, leads to a dramatic effect on border cell migration. 90% of border cell clusters migrate less than half way towards the oocyte. In 5% of egg chambers, border cell clusters are found off the direct track to the oocyte. The addition of vnEPg35521 to PvrDN.Scer\UAS (driven by Scer\GAL4slbo.2.6) leads to a strong enhancement of the border cell migration defect. Border cells do not reach the oocyte at stage 10. They usually migrate less than halfway, and are sometimes found off track.
The effect on border cell cluster migration when all border cells co-express PvrDN.Scer\UAS and EgfrDN.Scer\UAS under the control of Scer\GAL4slbo.2.6 and a single border cell expresses Egfr::Hsap\EGFRhE-E.Scer\UAS.T:Avic\GFP have been studied. In those border cell clusters where the single Egfr::Hsap\EGFRhE-E.Scer\UAS.T:Avic\GFP-expressing cell is located at the front of the cluster, the cluster on average moves forward, when the Egfr::Hsap\EGFRhE-E.Scer\UAS.T:Avic\GFP-expressing cell is in the back, the cluster on average moves relatively more backwards.
In the absence of illumination, border cells expressing EgfrDN.Scer\UAS and PvrDN.Scer\UAS under the control of Scer\GAL4slbo.2.6 and carrying Hsap\RAC1PA.Q61L.Scer\UAS.T:Disc\RFP-mCherry,T:Zzzz\PT1 fail to move forward. When Hsap\RAC1PA.Q61L.Scer\UAS.T:Disc\RFP-mCherry,T:Zzzz\PT1 is photoactivated at the rear of the border cell cluster, rearward movement is seen. When photoactivation is stopped, the border cell cluster stops moving.
Photo-inactivation of Hsap\RAC1PA.T17N.Scer\UAS.T:Disc\RFP-mCherry,T:Zzzz\PT1 at the front of border cell clusters expressing Hsap\RAC1PA.T17N.Scer\UAS.T:Disc\RFP-mCherry,T:Zzzz\PT1, EgfrDN.Scer\UAS and PvrDN.Scer\UAS under the control of Scer\GAL4slbo.2.6 results in local retraction.
The border follicle cell migration defects that are seen in flies co-expressing both PvrDN.Scer\UAS and hepAct.Scer\UAS under the control of Scer\GAL4slbo.2.6 are not suppressed by the co-expression of BacA\p35Scer\UAS.cHa.
The border follicle cell migration defect seen in egg chambers expressing PvrDN.Scer\UAS under the control of Scer\GAL4slbo.2.6 is not suppressed by coexpression of Mmus\Cd8aScer\UAS.T:Avic\GFP.
