Co-expression of Pvf1EPg11235 and PvrScer\UAS.T:Avic\GFP under the control of Scer\GAL4slbo.2.6 results in strong defects in border cell migration, with no migration being seen in approximately 60% of stage 10 egg chambers.
Border cells in the ovaries of Pvf1EPg11235; PvrDN.Scer\UAS; Scer\GAL4slbo.2.6 animals form lack long cellular extensions (20-40 μm) and form only very few intermediate cellular extensions (10-20 μm).
When PvrScer\UAS.cDa is added to Pvf1EPg11235, Scer\GAL4slbo.2.6 an enhancement of the border cell migration phenotype is seen. A quarter of the border cells at stage 10 are at the oocyte, but another quarter do not move at all. The addition of vnEPg35521 has no effect. the addition of Pvf1EPg11235 to EgfrDN.Scer\UAS when driven by Scer\GAL4slbo.2.6 leads to an enhancement of the border cell migration phenotype. Border cells do not reach the oocyte at stage 10. They usually migrate less than halfway, and are sometimes found off track.