Allele Dmel\ttk^69.Scer\UAS

General Information
Symbol	Dmel\ttk^69.Scer\UAS	Species	D. melanogaster
Name		FlyBase ID	FBal0127855
Feature type	allele	Associated gene	Dmel\ttk

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02	Controlled Vocabulary Terms interommatidial bristle ommatidium eye cone cell pupal stage photoreceptor cell visible adult stage
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Badenhorst, 2001)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of a full length ttk69 cDNA. (Badenhorst, 2001)
Carried in construct	P{UAS-ttk.69.B} (Badenhorst, 2001, Audibert et al., 2005, Baonza et al., 2002, Badenhorst et al., 2002, Bajpe et al., 2008)
Cytology
Phenotypic Data
Phenotypic Class
	visible, with Scer\GAL4^GMR.PF (Bajpe et al., 2008)
Phenotype Manifest In
	chemosensory ventral triple row bristle, with Scer\GAL4^Bx-MS1096 (Badenhorst et al., 2002) cone cell \| pupal stage, with Scer\GAL4^GMR.PF (Bajpe et al., 2008) dorsal triple row, with Scer\GAL4^Bx-MS1096 (Badenhorst et al., 2002) eye, with Scer\GAL4^GMR.PF (Bajpe et al., 2008) eye disc, with Scer\GAL4^GMR.PF (Baonza et al., 2002) eye disc \| pupal stage, with Scer\GAL4^GMR.PF (Bajpe et al., 2008) interface glial cell, with Scer\GAL4^Kr.PM (Badenhorst, 2001) interface glial cell, with Scer\GAL4^MZ1580 (Badenhorst, 2001) interface glial cell, with Scer\GAL4^sca-537.4 (Badenhorst, 2001) interommatidial bristle, with Scer\GAL4^GMR.PF (Bajpe et al., 2008) intersegmental nerve, with Scer\GAL4^elav-C155 (Badenhorst, 2001) longitudinal connective, with Scer\GAL4^elav-C155 (Badenhorst, 2001) medial triple row, with Scer\GAL4^Bx-MS1096 (Badenhorst et al., 2002) mitotic cell cycle, with Scer\GAL4^GMR.PF (Baonza et al., 2002) ommatidium \| adult stage, with Scer\GAL4^GMR.PF (Bajpe et al., 2008) ommatidium \| pupal stage, with Scer\GAL4^GMR.PF (Bajpe et al., 2008) photoreceptor cell \| pupal stage, with Scer\GAL4^GMR.PF (Bajpe et al., 2008) presumptive embryonic/larval central nervous system, with Scer\GAL4^elav-C155 (Badenhorst, 2001) sensory mother cell & dorsal mesothoracic disc, with Scer\GAL4^Bx-MS1096 (Badenhorst et al., 2002) ventral nerve cord primordium, with Scer\GAL4^Kr.PM (Badenhorst, 2001) ventral triple row, with Scer\GAL4^Bx-MS1096 (Badenhorst et al., 2002) wing, with Scer\GAL4^Bx-MS1096 (Badenhorst et al., 2002)
Detailed Description
	Statement Reference Expression of ttk[69.Scer\UAS] under the control of Scer\GAL4[GMR.PF] causes a distorted eye morphology characterized by disorganised facets and a loss of bristles. The cone cell pattern in the ommatidium of 40hr old pupal eye discs is also severely disrupted. The photoreceptor pattern is severely disrupted. (Bajpe et al., 2008) When ttk^69.Scer\UAS is driven by Scer\GAL4^Bx-MS1096 the wing blade area is reduced and all sensilla are removed except the ventral mechanosensory bristles. Sensory organ precursors are ablated in the wing disc. (Badenhorst et al., 2002) Expression of ttk^69.Scer\UAS under the control of Scer\GAL4^GMR.PF abolishes the second mitotic wave in the eye disc. The proportion of cells behind the furrow in G2 is increased in these discs compared to wild type. (Baonza et al., 2002) Expression of ttk^69.Scer\UAS under the control of Scer\GAL4^elav-C155 results in pathfinding and fasciculation defects in the central nervous system (CNS). The longitudinal fascicles are disorganised and the intersegmental nerve is misrouted and often fails to exit the CNS. Organisation of the peripheral nervous system is unaffected. Expression of ttk^69.Scer\UAS under the control of Scer\GAL4^Kr.PM inhibits normal glial development. Longitudinal glia are ablated when ttk^69.Scer\UAS is expressed under the control of Scer\GAL4^sca-537.4. The number of longitudinal glia is reduced when ttk^69.Scer\UAS is expressed under the control of Scer\GAL4^MZ1580. The normal BrdU incorporation in the ventral neuroectoderm is inhibited in embryos expressing ttk^69.Scer\UAS under the control of Scer\GAL4^Kr.PM. (Badenhorst, 2001)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, ttk^69.Scer\UAS has visible phenotype, enhanceable by Ubp64E^Scer\UAS.cBa/Scer\GAL4^GMR.PF (Bajpe et al., 2008)
NOT Enhanced by
Statement Reference Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, ttk^69.Scer\UAS has visible phenotype, non-enhanceable by Ubp64E^{C405A.Scer\UAS}/Scer\GAL4^GMR.PF (Bajpe et al., 2008)
Suppressed by
Statement Reference Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, ttk^69.Scer\UAS has visible phenotype, suppressible \| partially by Ubp64E[+]/Ubp64E^Δ1 (Bajpe et al., 2008)
NOT suppressed by
Statement Reference Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, ttk^69.Scer\UAS has visible phenotype, non-suppressible by Ubp64E[+]/Ubp64E^Rev (Bajpe et al., 2008)
Enhancer of
Statement Reference ttk^69.Scer\UAS/Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Scer\GAL4^GMR.PF, Ubp64E^Scer\UAS.cBa (Bajpe et al., 2008)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, ttk^69.Scer\UAS has eye disc \| pupal stage phenotype, enhanceable by Ubp64E^Scer\UAS.cBa/Scer\GAL4^GMR.PF (Bajpe et al., 2008) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, ttk^69.Scer\UAS has eye phenotype, enhanceable by Ubp64E^Scer\UAS.cBa/Scer\GAL4^GMR.PF (Bajpe et al., 2008) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, ttk^69.Scer\UAS has interommatidial bristle phenotype, enhanceable by Ubp64E^Scer\UAS.cBa/Scer\GAL4^GMR.PF (Bajpe et al., 2008) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, ttk^69.Scer\UAS has ommatidium \| adult stage phenotype, enhanceable by Ubp64E^Scer\UAS.cBa/Scer\GAL4^GMR.PF (Bajpe et al., 2008) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, ttk^69.Scer\UAS has ommatidium \| pupal stage phenotype, enhanceable by Ubp64E^Scer\UAS.cBa/Scer\GAL4^GMR.PF (Bajpe et al., 2008) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, ttk^69.Scer\UAS has photoreceptor cell \| pupal stage phenotype, enhanceable by Ubp64E^Scer\UAS.cBa/Scer\GAL4^GMR.PF (Bajpe et al., 2008) ttk^69.Scer\UAS has cone cell \| pupal stage phenotype, enhanceable by Ubp64E^Scer\UAS.cBa/Scer\GAL4^GMR.PF (Bajpe et al., 2008)
NOT Enhanced by
Statement Reference Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, ttk^69.Scer\UAS has cone cell \| pupal stage phenotype, non-enhanceable by Ubp64E^{C405A.Scer\UAS}/Scer\GAL4^GMR.PF (Bajpe et al., 2008) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, ttk^69.Scer\UAS has eye disc \| pupal stage phenotype, non-enhanceable by Ubp64E^{C405A.Scer\UAS}/Scer\GAL4^GMR.PF (Bajpe et al., 2008) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, ttk^69.Scer\UAS has eye phenotype, non-enhanceable by Ubp64E^{C405A.Scer\UAS}/Scer\GAL4^GMR.PF (Bajpe et al., 2008) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, ttk^69.Scer\UAS has interommatidial bristle phenotype, non-enhanceable by Ubp64E^{C405A.Scer\UAS}/Scer\GAL4^GMR.PF (Bajpe et al., 2008) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, ttk^69.Scer\UAS has ommatidium \| adult stage phenotype, non-enhanceable by Ubp64E^{C405A.Scer\UAS}/Scer\GAL4^GMR.PF (Bajpe et al., 2008) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, ttk^69.Scer\UAS has ommatidium \| pupal stage phenotype, non-enhanceable by Ubp64E^{C405A.Scer\UAS}/Scer\GAL4^GMR.PF (Bajpe et al., 2008) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, ttk^69.Scer\UAS has photoreceptor cell \| pupal stage phenotype, non-enhanceable by Ubp64E^{C405A.Scer\UAS}/Scer\GAL4^GMR.PF (Bajpe et al., 2008)
Suppressed by
Statement Reference Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, ttk^69.Scer\UAS has cone cell \| pupal stage phenotype, suppressible \| partially by Ubp64E[+]/Ubp64E^Δ1 (Bajpe et al., 2008) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, ttk^69.Scer\UAS has eye disc \| pupal stage phenotype, suppressible \| partially by Ubp64E[+]/Ubp64E^Δ1 (Bajpe et al., 2008) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, ttk^69.Scer\UAS has eye disc phenotype, suppressible by stg^Scer\UAS.cNa/Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Baonza et al., 2002) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, ttk^69.Scer\UAS has eye phenotype, suppressible \| partially by Ubp64E[+]/Ubp64E^Δ1 (Bajpe et al., 2008) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, ttk^69.Scer\UAS has interommatidial bristle phenotype, suppressible \| partially by Ubp64E[+]/Ubp64E^Δ1 (Bajpe et al., 2008) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, ttk^69.Scer\UAS has mitotic cell cycle phenotype, suppressible by stg^Scer\UAS.cNa/Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Baonza et al., 2002) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, ttk^69.Scer\UAS has ommatidium \| adult stage phenotype, suppressible \| partially by Ubp64E[+]/Ubp64E^Δ1 (Bajpe et al., 2008) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, ttk^69.Scer\UAS has ommatidium \| pupal stage phenotype, suppressible \| partially by Ubp64E[+]/Ubp64E^Δ1 (Bajpe et al., 2008) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, ttk^69.Scer\UAS has photoreceptor cell \| pupal stage phenotype, suppressible \| partially by Ubp64E[+]/Ubp64E^Δ1 (Bajpe et al., 2008)
NOT suppressed by
Statement Reference Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, ttk^69.Scer\UAS has cone cell \| pupal stage phenotype, non-suppressible by Ubp64E[+]/Ubp64E^Rev (Bajpe et al., 2008) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, ttk^69.Scer\UAS has eye disc \| pupal stage phenotype, non-suppressible by Ubp64E[+]/Ubp64E^Rev (Bajpe et al., 2008) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, ttk^69.Scer\UAS has eye phenotype, non-suppressible by Ubp64E[+]/Ubp64E^Rev (Bajpe et al., 2008) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, ttk^69.Scer\UAS has interommatidial bristle phenotype, non-suppressible by Ubp64E[+]/Ubp64E^Rev (Bajpe et al., 2008) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, ttk^69.Scer\UAS has ommatidium \| adult stage phenotype, non-suppressible by Ubp64E[+]/Ubp64E^Rev (Bajpe et al., 2008) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, ttk^69.Scer\UAS has ommatidium \| pupal stage phenotype, non-suppressible by Ubp64E[+]/Ubp64E^Rev (Bajpe et al., 2008) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, ttk^69.Scer\UAS has photoreceptor cell \| pupal stage phenotype, non-suppressible by Ubp64E[+]/Ubp64E^Rev (Bajpe et al., 2008)
Enhancer of
Statement Reference ttk^69.Scer\UAS/Scer\GAL4^GMR.PF is an enhancer of cone cell \| pupal stage phenotype of Scer\GAL4^GMR.PF, Ubp64E^Scer\UAS.cBa (Bajpe et al., 2008) ttk^69.Scer\UAS/Scer\GAL4^GMR.PF is an enhancer of eye disc \| pupal stage phenotype of Scer\GAL4^GMR.PF, Ubp64E^Scer\UAS.cBa (Bajpe et al., 2008) ttk^69.Scer\UAS/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, Ubp64E^Scer\UAS.cBa (Bajpe et al., 2008) ttk^69.Scer\UAS/Scer\GAL4^GMR.PF is an enhancer of interommatidial bristle phenotype of Scer\GAL4^GMR.PF, Ubp64E^Scer\UAS.cBa (Bajpe et al., 2008) ttk^69.Scer\UAS/Scer\GAL4^GMR.PF is an enhancer of ommatidium \| adult stage phenotype of Scer\GAL4^GMR.PF, Ubp64E^Scer\UAS.cBa (Bajpe et al., 2008) ttk^69.Scer\UAS/Scer\GAL4^GMR.PF is an enhancer of ommatidium \| pupal stage phenotype of Scer\GAL4^GMR.PF, Ubp64E^Scer\UAS.cBa (Bajpe et al., 2008) ttk^69.Scer\UAS/Scer\GAL4^GMR.PF is an enhancer of photoreceptor cell \| pupal stage phenotype of Scer\GAL4^GMR.PF, Ubp64E^Scer\UAS.cBa (Bajpe et al., 2008)
Additional Comments
Genetic Interactions
Statement Reference One copy of Ubp64E[Δ1] strongly suppresses the adult eye morphology phenotype seen when ttk[69.Scer\UAS] is expressed under the control of Scer\GAL4[GMR.PF]. The cone cell disruption see in pupal eye discs is also suppressed, as is the disruption to the photoreceptor pattern. One copy of Ubp64E[Rev] is unable to suppress the adult eye morphology phenotype seen when ttk[69.Scer\UAS] is expressed under the control of Scer\GAL4[GMR.PF]. The cone cell pattern disruption and loss of photoreceptors seen in pupal ommatidia is also unaffected. Co-expression of ttk[69.Scer\UAS] and Ubp64E[Scer\UAS.cBa] under the control of Scer\GAL4[GMR.PF] causes dramatically defective eye development, more severe than is seen when either transgene is expressed alone. The cone cell disruption seen in the ommatidia of pupal eye discs is also enhanced, as is the disruption to the photoreceptor pattern. Expression of Ubp64E[C405A.Scer\UAS] does not enhance the adult eye phenotypes seen when ttk[69.Scer\UAS] is expressed under the control of Scer\GAL4[GMR.PF]. The cone cell and photoreceptor disruption seen in the ommatidia of pupal eye discs is also unaffected. (Bajpe et al., 2008) Coexpression of stg^Scer\UAS.cNa completely rescues the second mitotic wave in eye discs expressing ttk^69.Scer\UAS under the control of Scer\GAL4^GMR.PF. (Baonza et al., 2002)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	ttk^69.Scer\UAS
Name Synonym
Secondary FlyBase IDs

References ( 5 )
Research paper	Bajpe et al., 2008, Mol. Cell. Biol. 28(5): 1606--1615 Deubiquitylating enzyme UBP64 controls cell fate through stabilization of the transcriptional repressor tramtrack. [FBrf0204221] Audibert et al., 2005, Development 132(10): 2287--2297 Cell cycle diversity involves differential regulation of Cyclin E activity in the Drosophila bristle cell lineage. [FBrf0187449] Badenhorst et al., 2002, Mech. Dev. 117(1-2): 87--101 Tramtrack co-operates to prevent inappropriate neural development in Drosophila. [FBrf0152198] Baonza et al., 2002, Nat. Cell Biol. 4(12): 976--980 Pointed and Tramtrack69 establish an EGFR-dependent transcriptional switch to regulate mitosis. [FBrf0155467] Badenhorst, 2001, Development 128(20): 4093--4101 Tramtrack controls glial number and identity in the Drosophila embryonic CNS. [FBrf0139635]

Allele Dmel\ttk69.Scer\UAS

Allele Dmel\ttk^69.Scer\UAS