FlyBase Allele Report: Dmel\PlexB[UAS.cHa]

General Information

Symbol

Dmel\PlexB^UAS.cHa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Hu

FlyBase ID

FBal0127907

Feature type

allele

Associated gene

Dmel\PlexB

Associated Insertion(s)

Carried in Construct

P{UAS-PlexB.H}

Also Known As

UAS-PlexB

Key Links

Transgenic product class

wild_type

(Hu et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Hu et al., 2001)

Mutagen

in vitro construct

(Hu et al., 2001)

Progenitor genotype

Carried in construct

P{UAS-PlexB.H}

Cytology

Description

UASt sequences drive expression of full length PlexB.

(Hu et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

PlexB

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

larval intersegmental nerve, with Scer\GAL4^elav.PLu

(Hu et al., 2001)

larval VL3/4 motor neuron, with Scer\GAL4^elav.PLu

(Hu et al., 2001)

Detailed Description

Statement

Reference

When plexB^Scer\UAS.cHa is driven by Scer\GAL4^elav.PLu, a specific phenotyper is seen in the motor nerve branches. A striking defect is seen in the ability of the ISNb motor axons to innervate the ventral longitudinal muscles 7, 6, 13, and 12. The RP3 axon frequently fails to defasciculate from the ISNb motor nerve branch as a result muscles 7 and 6 are uninnervated. ISNb axons often fail to reach their distal-most target muscle 12. These phenotypes are dose dependant.

(Hu et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

PlexB^UAS.cHa, Scer\GAL4^elav.PLu has larval VL3/4 motor neuron phenotype, enhanceable by Df(3L)Ar14-8

(Hu et al., 2001)

PlexB^UAS.cHa, Scer\GAL4^elav.PLu has larval intersegmental nerve phenotype, enhanceable by Df(3L)Ar14-8

(Hu et al., 2001)

PlexB^UAS.cHa, Scer\GAL4^elav.PLu has larval VL3/4 motor neuron phenotype, enhanceable by trio^S137203

(Hu et al., 2001)

PlexB^UAS.cHa, Scer\GAL4^elav.PLu has larval intersegmental nerve phenotype, enhanceable by trio^S137203

(Hu et al., 2001)

NOT Enhanced by

Statement

Reference

PlexB^UAS.cHa, Scer\GAL4^elav.PLu has larval VL3/4 motor neuron phenotype, non-enhanceable by Cdc42³

(Hu et al., 2001)

PlexB^UAS.cHa, Scer\GAL4^elav.PLu has larval intersegmental nerve phenotype, non-enhanceable by Cdc42³

(Hu et al., 2001)

PlexB^UAS.cHa, Scer\GAL4^elav.PLu has larval VL3/4 motor neuron phenotype, non-enhanceable by Df(3L)ru-22

(Hu et al., 2001)

PlexB^UAS.cHa, Scer\GAL4^elav.PLu has larval intersegmental nerve phenotype, non-enhanceable by Df(3L)ru-22

(Hu et al., 2001)

Suppressed by

Statement

Reference

PlexB^UAS.cHa, Scer\GAL4^elav.PLu has larval intersegmental nerve phenotype, suppressible by Pak^UAS.cHa, Scer\GAL4^elav.PLu

(Hu et al., 2001)

PlexB^UAS.cHa, Scer\GAL4^elav.PLu has larval intersegmental nerve phenotype, suppressible by Rac1^UAS.cLa, Scer\GAL4^elav.PLu

(Hu et al., 2001)

PlexB^UAS.cHa, Scer\GAL4^elav.PLu has larval VL3/4 motor neuron phenotype, suppressible by Rho1^k07236

(Hu et al., 2001)

PlexB^UAS.cHa, Scer\GAL4^elav.PLu has larval VL3/4 motor neuron phenotype, suppressible by Rho1^rev220

(Hu et al., 2001)

PlexB^UAS.cHa, Scer\GAL4^elav.PLu has larval intersegmental nerve phenotype, suppressible by Rho1^k07236

(Hu et al., 2001)

PlexB^UAS.cHa, Scer\GAL4^elav.PLu has larval intersegmental nerve phenotype, suppressible by Rho1^rev220

(Hu et al., 2001)

PlexB^UAS.cHa, Scer\GAL4^elav.PLu has larval VL3/4 motor neuron phenotype, suppressible by Pak^UAS.cHa, Scer\GAL4^elav.PLu

(Hu et al., 2001)

PlexB^UAS.cHa, Scer\GAL4^elav.PLu has larval VL3/4 motor neuron phenotype, suppressible by Rac1^UAS.cLa, Scer\GAL4^elav.PLu

(Hu et al., 2001)

NOT suppressed by

Statement

Reference

PlexB^UAS.cHa, Scer\GAL4^elav.PLu has larval VL3/4 motor neuron phenotype, non-suppressible by Cdc42³

(Hu et al., 2001)

PlexB^UAS.cHa, Scer\GAL4^elav.PLu has larval VL3/4 motor neuron phenotype, non-suppressible by Df(3L)ru-22

(Hu et al., 2001)

PlexB^UAS.cHa, Scer\GAL4^elav.PLu has larval intersegmental nerve phenotype, non-suppressible by Cdc42³

(Hu et al., 2001)

PlexB^UAS.cHa, Scer\GAL4^elav.PLu has larval intersegmental nerve phenotype, non-suppressible by Df(3L)ru-22

(Hu et al., 2001)

Enhancer of

Statement

Reference

PlexB^UAS.cHa, Scer\GAL4^elav.PLu is an enhancer of larval intersegmental nerve phenotype of Rac1^N17.UAS, Scer\GAL4^elav.PLu

(Hu et al., 2001)

PlexB^UAS.cHa, Scer\GAL4^elav.PLu is an enhancer of larval abdominal segmental nerve phenotype of Rac1^N17.UAS, Scer\GAL4^elav.PLu

(Hu et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of plexB^Scer\UAS.cHa under the control of Scer\GAL4^elav-C155 is unable to rescue any of the ISNb, SNa or CNS defects observed in Df(4)C3 mutants.

Df(4)C3 defects, affecting the ISNb andSNa are enhanced by 19% and 25% respectively, when plexB^Scer\UAS.cHa is expressed (under the control of Scer\GAL4^elav-C155).

(Ayoob et al., 2006)

The Intersegmental nerve and RP3 nerve phenotypes have an increased penetrance when Df(3L)Ar14-8 or trio^S137203 is added to plexB^Scer\UAS.cHa, Scer\GAL4^elav.PLu flies. The addition of Df(3L)ru-22 has no effect. the addition of Rac1^Scer\UAS.cLa or Pak^Scer\UAS.cHa to plexB^Scer\UAS.cHa, Scer\GAL4^elav.PLu flies suppresses the intersegmental nerve and RP3 nerve phenotypes. The addition of Cdc42³ to plexB^Scer\UAS.cHa, Scer\GAL4^elav.PLu files has no effect on the intersegmental nerve and RP3 nerve phenotypes. the addition of Rho1^rev220 or Rho1^k07236 to plexB^Scer\UAS.cHa, Scer\GAL4^elav.PLu flies suppresses the intersegmental nerve and RP3 nerve phenotypes. The addition of plexB^Scer\UAS.cHa to Rac1^N17.Scer\UAS, Scer\GAL4^elav.PLu flies enhances the ISNb bypass phenotype seen in these flies.

(Hu et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

PlexB^UAS.cHa/Scer\GAL4^peb-GAL4 rescues PlexB^KG00878

(Joo et al., 2013)

PlexB^UAS.cHa/Scer\GAL4^elav-C155 rescues PlexB^KG00878

(Ayoob et al., 2006)

Partially rescues

Scer\GAL4^{PlexB-MI15559-TG4.1}/PlexB^UAS.cHa partially rescues PlexB^KG00878/PlexB^{MI15559-TG4.1}

(Xie et al., 2019)

Comments

Expression of PlexB^UAS.cHa under the control of Scer\GAL4^{PlexB-MI15559-TG4.1} almost completely rescues prior to eclosion lethality and partially rescues after eclosion lethality phenotypes of PlexB^KG00878/PlexB^{MI15559-TG4.1} transheterozygotes.

(Xie et al., 2019)

Expression of plexB^Scer\UAS.cHa in all neurons in a plexB^KG00878 homozygous background, under the control of Scer\GAL4^elav-C155, significantly rescues the intersegmental nerve b (ISNb) motor axon defects. Restoring plexB to all neurons also rescues the lethality associated with the plexB^KG00878 mutants (16.8% homozygous viable progeny compared to 5.7% of the plexB^KG00878/+ x Df(4)M101-62f/+ crossing being homozygous viable progeny).

Expression of plexB^Scer\UAS.cHa in all neurons in a plexB^KG00878 homozygous background, under the control of Scer\GAL4^elav-C155, significantly reduces the number of SNa defects by over 50%.

Expression of plexB^Scer\UAS.cHa in all neurons in a plexB^KG00878 homozygous background, under the control of Scer\GAL4^elav-C155 rescues the severe defasciculation of the medial Fas2-stained longitudinal fascicle.

(Ayoob et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

PlexB^Scer\UAS.cHa

PlexB^UAS.cHa

plexB^Scer\UAS.cHa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Hu

(Hu et al., 2001)

Secondary FlyBase IDs

References (8)

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