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General Information
Symbol
Dmel\PlexBUAS.cHa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Hu
FlyBase ID
FBal0127907
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-PlexB
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt sequences drive expression of full length PlexB.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

When plexBScer\UAS.cHa is driven by Scer\GAL4elav.PLu, a specific phenotyper is seen in the motor nerve branches. A striking defect is seen in the ability of the ISNb motor axons to innervate the ventral longitudinal muscles 7, 6, 13, and 12. The RP3 axon frequently fails to defasciculate from the ISNb motor nerve branch as a result muscles 7 and 6 are uninnervated. ISNb axons often fail to reach their distal-most target muscle 12. These phenotypes are dose dependant.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
Additional Comments
Genetic Interactions
Statement
Reference

Expression of plexBScer\UAS.cHa under the control of Scer\GAL4elav-C155 is unable to rescue any of the ISNb, SNa or CNS defects observed in Df(4)C3 mutants.

Df(4)C3 defects, affecting the ISNb andSNa are enhanced by 19% and 25% respectively, when plexBScer\UAS.cHa is expressed (under the control of Scer\GAL4elav-C155).

The Intersegmental nerve and RP3 nerve phenotypes have an increased penetrance when Df(3L)Ar14-8 or trioS137203 is added to plexBScer\UAS.cHa, Scer\GAL4elav.PLu flies. The addition of Df(3L)ru-22 has no effect. the addition of Rac1Scer\UAS.cLa or PakScer\UAS.cHa to plexBScer\UAS.cHa, Scer\GAL4elav.PLu flies suppresses the intersegmental nerve and RP3 nerve phenotypes. The addition of Cdc423 to plexBScer\UAS.cHa, Scer\GAL4elav.PLu files has no effect on the intersegmental nerve and RP3 nerve phenotypes. the addition of Rho1rev220 or Rho1k07236 to plexBScer\UAS.cHa, Scer\GAL4elav.PLu flies suppresses the intersegmental nerve and RP3 nerve phenotypes. The addition of plexBScer\UAS.cHa to Rac1N17.Scer\UAS, Scer\GAL4elav.PLu flies enhances the ISNb bypass phenotype seen in these flies.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of PlexBUAS.cHa under the control of Scer\GAL4PlexB-MI15559-TG4.1 almost completely rescues prior to eclosion lethality and partially rescues after eclosion lethality phenotypes of PlexBKG00878/PlexBMI15559-TG4.1 transheterozygotes.

Expression of plexBScer\UAS.cHa in all neurons in a plexBKG00878 homozygous background, under the control of Scer\GAL4elav-C155, significantly rescues the intersegmental nerve b (ISNb) motor axon defects. Restoring plexB to all neurons also rescues the lethality associated with the plexBKG00878 mutants (16.8% homozygous viable progeny compared to 5.7% of the plexBKG00878/+ x Df(4)M101-62f/+ crossing being homozygous viable progeny).

Expression of plexBScer\UAS.cHa in all neurons in a plexBKG00878 homozygous background, under the control of Scer\GAL4elav-C155, significantly reduces the number of SNa defects by over 50%.

Expression of plexBScer\UAS.cHa in all neurons in a plexBKG00878 homozygous background, under the control of Scer\GAL4elav-C155 rescues the severe defasciculation of the medial Fas2-stained longitudinal fascicle.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
PlexBScer\UAS.cHa
PlexBUAS.cHa
plexBScer\UAS.cHa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Hu
Secondary FlyBase IDs
    References (7)