FB2025_01 , released February 20, 2025
Allele: Dmel\otkUAS.cWa
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General Information
Symbol
Dmel\otkUAS.cWa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Winberg
FlyBase ID
FBal0127917
Feature type
allele
Associated gene
Dmel\otk
Associated Insertion(s)
Carried in Construct
P{UAS-otk.W}
Also Known As
UAS-otk
Key Links
Transgenic product class
cDNA
(Winberg et al., 2001)
wild_type
(Winberg et al., 2001)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Winberg et al., 2001)
wild_type
(Winberg et al., 2001)
Mutagen
in vitro construct
(Winberg et al., 2001)
Progenitor genotype
Carried in construct
P{UAS-otk.W}
Cytology
Description

UASt sequences drive expression of a full length otk cDNA plus 241 nts of 5'UTR and 14 nts of 3'UTR.

(Winberg et al., 2001)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
otk
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of otkScer\UAS.cWa under the control of Scer\GAL4da.G32 results in ectopic rows of denticles.

      Expression of otkScer\UAS.cWa under the control of Scer\GAL4ptc-559.1 results in a loss of wing margin. The wings also show planar cell polarity defects where wing hairs are not properly aligned.

      (Peradziryi et al., 2011)

      Targetting of photoreceptor cell R7 axons to the medulla is normal in otkScer\UAS.cWa; Scer\GAL4sev.PM181 adults.

      (Cafferty et al., 2004)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of Wnt4Scer\UAS.cGa and otkScer\UAS.cWa under the control of Scer\GAL4da.G32 results in embryos where all epidermal cells secrete denticles.

      (Peradziryi et al., 2011)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      otkUAS.cWa/Scer\GAL4GMR.PF rescues otkEP2017/otk3

      (Cafferty et al., 2004)

      otkUAS.cWa/Scer\GAL4elav.PLu rescues otk3

      (Winberg et al., 2001)
      Comments

      Mistargetting of developing eye photoreceptor axons in otk3/otkEP2017 late third instar larvae is largely suppressed by otkScer\UAS.cWa; Scer\GAL4GMR.PF.

      (Cafferty et al., 2004)

      The addition of otkScer\UAS.cWa driven by Scer\GAL4elav.PLu to otk3 animals restores the axon guidance phenotypes, including restoring the CNS morphology.

      (Winberg et al., 2001)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      otkScer\UAS.cWa
      otkUAS.cWa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Winberg
      (Winberg et al., 2001)
      Secondary FlyBase IDs
        References (4)