FlyBase Allele Report: Dmel\otk[EP2017]

General Information

Symbol

Dmel\otk^EP2017

Species

D. melanogaster

Name

FlyBase ID

FBal0127918

Feature type

allele

Associated gene

Dmel\otk

Associated Insertion(s)

P{EP}otk^EP2017

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Winberg et al., 2001)

Progenitor genotype

Associated Insertion(s)

P{EP}otk^EP2017

Cytology

Description

The P{EP} insertion is 30bp upstream of the 5' end of the otk cDNA, and within the 5' UTR inferred from transcript size. Its orientation is opposite to the open reading frame.

(Winberg et al., 2001)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:12,019,847..12,019,847 [+]

(Gene Disruption Project members, 2001-, Winberg et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

partially lethal - majority die, with Scer\GAL4^elav.PLu

(Winberg et al., 2001)

Phenotype Manifest In

axon, with Scer\GAL4^elav.PLu

(Winberg et al., 2001)

larval abdominal segmental nerve, with Scer\GAL4^elav.PLu

(Winberg et al., 2001)

larval intersegmental nerve, with Scer\GAL4^elav.PLu

(Winberg et al., 2001)

photoreceptor cell R2 & axon & larva (with otk³)

(Cafferty et al., 2004)

photoreceptor cell R3 & axon & larva (with otk³)

(Cafferty et al., 2004)

photoreceptor cell R4 & axon & larva (with otk³)

(Cafferty et al., 2004)

photoreceptor cell R5 & axon & larva (with otk³)

(Cafferty et al., 2004)

photoreceptor cell R6 & axon & larva (with otk³)

(Cafferty et al., 2004)

Detailed Description

Statement

Reference

In late third instar otk³/otk^EP2017 there are minor defects in axonal projections from developing eye photoreceptor cells: an average of 18 R2-R5 axons or axon bundles per hemisphere are mistargeted.

(Cafferty et al., 2004)

When otk^EP2017 is driven by Scer\GAL4^elav.PLu axon guidance phenotypes are seen. The dorsal branch of the SNa often does not divide in the proper location but rather extends as a single thicker branch, remaining fasciculated together in over 60% of segments. The ISNb can sometimes fail to defasciculate from the ISN, and the branches from the ISNb to individual muscles are also sometimes bypassed.

(Winberg et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

otk^EP2017/otk³ is rescued by otk^UAS.cWa/Scer\GAL4^GMR.PF

(Cafferty et al., 2004)

Comments

Mistargetting of developing eye photoreceptor axons in otk³/otk^EP2017 late third instar larvae is largely suppressed by otk^Scer\UAS.cWa; Scer\GAL4^GMR.PF.

(Cafferty et al., 2004)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

17231

w¹¹¹⁸; P{EP}otk^EP2017/CyO

Kyoto

114461

y^d2 w¹¹¹⁸ P{ey-FLP.N}2; P{neoFRT}42D P{EP}otk^EP2017 /CyO y⁺

Notes on Origin