FB2021_01, released Feb 18, 2021

Allele: Dmel\jebUAS.cWa

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General Information
Symbol
Dmel\jebUAS.cWa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Weiss
FlyBase ID
FBal0127956
Feature type
allele
Associated gene
Dmel\jeb
Associated Insertion(s)
Carried in Construct
P{UAS-jeb.W}
Also Known As
UAS-jeb
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
in vitro construct - regulatory fusion
(Weiss et al., 2001)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Carried in construct
      P{UAS-jeb.W}
      Cytology
      Nature of the lesion
      Statement
      Reference

      UASt regulatory sequences drive expression of a jeb cDNA.

      (Weiss et al., 2001)
      Allele components
      Product class / Tool use(s)
      Regulatory region(s)
      UASt
      Encoded product / tool
      jeb
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4how-24B

      (Rohrbough et al., 2013)

      fusion competent cell & visceral mesoderm, with Scer\GAL4twi.PG

      (Stute et al., 2004)

      muscle founder cell & visceral mesoderm, with Scer\GAL4twi.PG

      (Stute et al., 2004)

      muscle founder cell & visceral muscle primordium | ectopic, with Scer\GAL4twi.2PE

      (Lee et al., 2003)

      visceral mesoderm, with Scer\GAL4bap.3

      (Weiss et al., 2001)
      Detailed Description
      Statement
      Reference

      Expression of jebScer\UAS.cWa under the control of Scer\GAL4how-24B significantly reduces mean excitatory junctional current (EJC) amplitude (30% reduction) and excitatory junctional potential (EJP) amplitude (42% reduction) at the larval neuromuscular junction compared to controls. EJP amplitude is reduced by 13% in larvae expressing jebScer\UAS.cWa under the control of Scer\GAL4futsch-C380. EJC amplitude is normal in larvae expressing jebScer\UAS.cWa under the control of Scer\GAL4elav.PLu.

      Neuromuscular junction (NMJ) area per muscle area is significantly decreased compared to wild type in third instar larvae expressing jebScer\UAS.cWa under the control of Scer\GAL4how-24B. Bouton number per NMJ area is significantly increased and bouton number per muscle area is not significantly different from wild type.

      (Rohrbough et al., 2013)

      Marker analysis indicates that in jebScer\UAS.cWa; Scer\GAL4twi.PG embryos, nearly all of the cells of the visceral mesoderm become muscle founder cells, and fusion competent myoblasts are lacking. Despite this, circular visceral muscles differentiate in these animals.

      (Stute et al., 2004)

      Marker analysis shows that jebScer\UAS.cWa; Scer\GAL4twi.2PE embryos have increased numbers of visceral muscle founder cells at stage 11-12.

      (Lee et al., 2003)

      Expression of jebScer\UAS.cWa under the control of Scer\GAL4bap.3 produces visceral mesoderm defects.

      (Weiss et al., 2001)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4twi.PG, Scer\GAL4how-24B, Scer\GAL4how-24B, jebUAS.cWa rescues jebunspecified

      (Weiss et al., 2001)
      Partially rescues

      Scer\GAL4bap.3/jebUAS.cWa partially rescues jebunspecified

      (Weiss et al., 2001)
      Comments

      Expression of jebScer\UAS.cWa under the control of Scer\GAL4bap.3 rescues early visceral mesoderm development in jebunspecified mutant embryos. Subsequent visceral mesoderm migration is often abnormal in these embryos, with longitudinal migration to form continuous bands being incomplete.

      (Weiss et al., 2001)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      jebScer\UAS.cWa
      jebUAS.cWa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Weiss
      (Weiss et al., 2001)
      Secondary FlyBase IDs
        References (5)