Open Close
General Information
Symbol
Dmel\jebUAS.cWa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Weiss
FlyBase ID
FBal0127956
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-jeb
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a jeb cDNA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

fusion competent cell & visceral mesoderm, with Scer\GAL4twi.PG

muscle founder cell & visceral mesoderm, with Scer\GAL4twi.PG

muscle founder cell & visceral muscle primordium | ectopic, with Scer\GAL4twi.2PE

Detailed Description
Statement
Reference

Expression of jebScer\UAS.cWa under the control of Scer\GAL4how-24B significantly reduces mean excitatory junctional current (EJC) amplitude (30% reduction) and excitatory junctional potential (EJP) amplitude (42% reduction) at the larval neuromuscular junction compared to controls. EJP amplitude is reduced by 13% in larvae expressing jebScer\UAS.cWa under the control of Scer\GAL4futsch-C380. EJC amplitude is normal in larvae expressing jebScer\UAS.cWa under the control of Scer\GAL4elav.PLu.

Neuromuscular junction (NMJ) area per muscle area is significantly decreased compared to wild type in third instar larvae expressing jebScer\UAS.cWa under the control of Scer\GAL4how-24B. Bouton number per NMJ area is significantly increased and bouton number per muscle area is not significantly different from wild type.

Marker analysis indicates that in jebScer\UAS.cWa; Scer\GAL4twi.PG embryos, nearly all of the cells of the visceral mesoderm become muscle founder cells, and fusion competent myoblasts are lacking. Despite this, circular visceral muscles differentiate in these animals.

Marker analysis shows that jebScer\UAS.cWa; Scer\GAL4twi.2PE embryos have increased numbers of visceral muscle founder cells at stage 11-12.

Expression of jebScer\UAS.cWa under the control of Scer\GAL4bap.3 produces visceral mesoderm defects.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescues
Comments

Expression of jebScer\UAS.cWa under the control of Scer\GAL4bap.3 rescues early visceral mesoderm development in jebunspecified mutant embryos. Subsequent visceral mesoderm migration is often abnormal in these embryos, with longitudinal migration to form continuous bands being incomplete.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
jebScer\UAS.cWa
jebUAS.cWa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Weiss
Secondary FlyBase IDs
    References (5)