Expression of jebScer\UAS.cWa under the control of Scer\GAL4how-24B significantly reduces mean excitatory junctional current (EJC) amplitude (30% reduction) and excitatory junctional potential (EJP) amplitude (42% reduction) at the larval neuromuscular junction compared to controls. EJP amplitude is reduced by 13% in larvae expressing jebScer\UAS.cWa under the control of Scer\GAL4futsch-C380. EJC amplitude is normal in larvae expressing jebScer\UAS.cWa under the control of Scer\GAL4elav.PLu.
Neuromuscular junction (NMJ) area per muscle area is significantly decreased compared to wild type in third instar larvae expressing jebScer\UAS.cWa under the control of Scer\GAL4how-24B. Bouton number per NMJ area is significantly increased and bouton number per muscle area is not significantly different from wild type.
Marker analysis indicates that in jebScer\UAS.cWa; Scer\GAL4twi.PG embryos, nearly all of the cells of the visceral mesoderm become muscle founder cells, and fusion competent myoblasts are lacking. Despite this, circular visceral muscles differentiate in these animals.