Allele Dmel\hbs^Scer\UAS.cDa

General Information
Symbol	Dmel\hbs^Scer\UAS.cDa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Dworak	FlyBase ID	FBal0127973
Feature type	allele	Associated gene	Dmel\hbs

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Dworak et al., 2001)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS sequences drive expression of the longest hbs cDNA (cDNA4, which is 4712bp long). (Dworak et al., 2001)
Carried in construct	P{UAS-hbs.D} (Dworak et al., 2001, Bao and Cagan, 2005, Bao et al., 2010, Weber, 2012.9.7, Singh and Mlodzik, 2012)
Cytology
Phenotypic Data
Phenotypic Class
	cell adhesion defective \| somatic clone, with Scer\GAL4^GMR.PF (Bao et al., 2010) planar polarity defective, with Scer\GAL4^hs.2sev (Singh and Mlodzik, 2012) visible, with Scer\GAL4^bi-md653 (Dworak et al., 2001) visible, with Scer\GAL4^da.G32 (Dworak et al., 2001) visible, with Scer\GAL4^en-e16E (Singh and Mlodzik, 2012) visible, with Scer\GAL4^GMR.PF (Dworak et al., 2001) visible, with Scer\GAL4^pnr-MD237 (Dworak et al., 2001) visible, with Scer\GAL4^sca-537.4 (Dworak et al., 2001)
Phenotype Manifest In
	cell-cell junction \| somatic clone, with Scer\GAL4^Act5C.PI (Bao and Cagan, 2005) eye, with Scer\GAL4^da.G32 (Dworak et al., 2001) eye, with Scer\GAL4^GMR.PF (Dworak et al., 2001) eye, with Scer\GAL4^hs.2sev (Singh and Mlodzik, 2012) eye, with Scer\GAL4^sca-537.4 (Dworak et al., 2001) eye \| somatic clone, with Scer\GAL4^GMR.PF (Bao et al., 2010) eye photoreceptor cell, with Scer\GAL4^hs.2sev (Singh and Mlodzik, 2012) interommatidial bristle, with Scer\GAL4^da.G32 (Dworak et al., 2001) interommatidial bristle, with Scer\GAL4^GMR.PF (Dworak et al., 2001) interommatidial bristle, with Scer\GAL4^sca-537.4 (Dworak et al., 2001) inter-ommatidial cell \| somatic clone, with Scer\GAL4^GMR.PF (Bao et al., 2010) inter-ommatidial precursor cell \| P-stage \| somatic clone, with Scer\GAL4^Act5C.PI (Bao and Cagan, 2005) inter-ommatidial precursor cell \| somatic clone, with Scer\GAL4^GMR.PF (Bao et al., 2010) microchaeta & scutum, with Scer\GAL4^da.G32 (Dworak et al., 2001) microchaeta & scutum, with Scer\GAL4^pnr-MD237 (Dworak et al., 2001) microchaeta & scutum, with Scer\GAL4^sca-537.4 (Dworak et al., 2001) myoblast, with Scer\GAL4^da.G32 (Dworak et al., 2001) myoblast, with Scer\GAL4^en-e16E (Dworak et al., 2001) myoblast, with Scer\GAL4^pnr-MD237 (Dworak et al., 2001) myoblast, with Scer\GAL4^sca-537.4 (Dworak et al., 2001) myoblast, with Scer\GAL4^twi.PG (Dworak et al., 2001) ommatidium, with Scer\GAL4^da.G32 (Dworak et al., 2001) ommatidium, with Scer\GAL4^GMR.PF (Dworak et al., 2001) ommatidium, with Scer\GAL4^hs.2sev (Singh and Mlodzik, 2012) ommatidium, with Scer\GAL4^sca-537.4 (Dworak et al., 2001) ommatidium \| somatic clone, with Scer\GAL4^GMR.PF (Bao et al., 2010) presumptive embryonic/larval muscle system, with Scer\GAL4^da.G32 (Dworak et al., 2001) presumptive embryonic/larval muscle system, with Scer\GAL4^en-e16E (Dworak et al., 2001) presumptive embryonic/larval muscle system, with Scer\GAL4^sca-537.4 (Dworak et al., 2001) presumptive embryonic/larval muscle system \| dorsal, with Scer\GAL4^pnr-MD237 (Dworak et al., 2001) primary pigment cell \| P-stage \| somatic clone, with Scer\GAL4^Act5C.PI (Bao and Cagan, 2005) septate junction \| somatic clone, with Scer\GAL4^Act5C.PI (Bao and Cagan, 2005) wing margin, with Scer\GAL4^en-e16E (Singh and Mlodzik, 2012) wing margin \| distal, with Scer\GAL4^bi-md653 (Dworak et al., 2001) wing margin \| distal, with Scer\GAL4^da.G32 (Dworak et al., 2001)
Detailed Description
	Statement Reference Overexpression of hbs[Scer\UAS.cDa] under the control of Scer\GAL4[hs.2sev] results in eye planar cell polarity (PCP) defects including chirality and ommatidial rotation defects and also some photoreceptor cell loss. Overexpression of hbs[Scer\UAS.cDa] in the posterior compartment of the wing under the control of Scer\GAL4[en-e16E] results in wing notching. (Singh and Mlodzik, 2012) Clonal expression of a single copy of hbs[Scer\UAS.cDa] within an inter-ommatidial cell (under the control of Scer\GAL4[GMR.PF]) leads to partial segregation of the hbs-expressing inter-ommatidial cell. Typically, the hbs-targeted inter-ommatidial cell retains contact with one ommatidium and is flattened against it but is segregated away from the second ommatidium by neighboring inter-ommatidial cells, suggesting reduced adhesion between the hbs-targeted inter-ommatidial cell and ommatidia. Interestingly, the neighboring inter-ommatidial cells are part of the larger inter-ommatidial cell hexagonal lattice, which establishes across the hbs-targeted inter-ommatidial cell as if it is part of the adjacent ommatidium. Neighboring inter-ommatidial cells establish abnormally large interfaces with the hbs-targeted inter-ommatidial cell, suggesting adhesion between the hbs-targeted inter-ommatidial cell and neighboring inter-ommatidial cells is stronger than adhesion normally observed between inter-ommatidial cells. (Bao et al., 2010) Primary pigment cell precursors in hbs[Scer\UAS.cDa]; Scer\GAL4[Act5C.PI] somatic clones are expanded compared to adjacent wild-type primary pigment cell precursors. When these somatic clones are induced at 18 hours APF, the clones have a reduced apical profile and are excluded from the flow of the ommatidial lattice. When these somatic clones are induced at 3-6 hours APF, inter-ommatidial precursor cells (IPCs) within clones have an abnormally large border and straight with neighboring wild-type IPCs. These cells also form ectopic septate junctions. When hbs is expressed ubiquitously using hbs[Scer\UAS.cDa]; Scer\GAL4[hs.PB] with heat shock at 25 hours APF, the polarized pattern of cell junctions of inter-ommatidial precursor cells at 27 hours is disrupted. (Bao and Cagan, 2005) When hbs^Scer\UAS.cDa is driven by Scer\GAL4^elav.PLu, Scer\GAL4^da.G32 or Scer\GAL4^sca-537.4 no defects are seen in axon guidance, or in glial cell placement and number. When hbs^Scer\UAS.cDa is driven by Scer\GAL4^twi.PG myoblast fusion is partly disrupted in all hemisegments, but muscle fibre number and sites of muscle attachment are not. When hbs^Scer\UAS.cDa is driven by Scer\GAL4^da.G32 myoblast fusion is partly disrupted in all hemisegments. Some muscle fibres are also inserted at inappropriate attachment locations. When hbs^Scer\UAS.cDa is driven by Scer\GAL4^en-e16E disrupts attachments made by several lateral muscle fibres in most hemi-segments of all embryos. When driven by Scer\GAL4^sca-537.4 disrupted attachments are seen in all hemisegments of all embryos. When driven by Scer\GAL4^pnr-MD237 dorsal muscle attachments sites are radically disrupted, with the muscle fibres often failing to cross the segment and instead aligning with the segment boundary. Un-fused myoblasts are also seen in these combinations. When hbs^Scer\UAS.cDa is driven by Scer\GAL4^da.G32 or Scer\GAL4^bi-md653 an abnormal distal wing margin is seen. When driven by Scer\GAL4^bi-md653 the distal wing edge is blistered, when driven by hbs^Scer\UAS.cDa it is blistered or notched. When hbs^Scer\UAS.cDa is driven by Scer\GAL4^pnr-MD237, Scer\GAL4^sca-537.4 or Scer\GAL4^da.G32 the arrangement, but not the numbers of microchaetae on the central scutum is altered. When hbs^Scer\UAS.cDa is driven by Scer\GAL4^GMR.PF or Scer\GAL4^sca-537.4 a rough eye phenotype is seen - ommatidia and bristles are disorganised. When driven by Scer\GAL4^da.G32, a strong rough eye phenotype is seen., with ommatidia occasionally fusing. Te photoreceptor clusters are irregularly placed in these adults and pigment cells are absent at sites of ommatidia fusion, though larval pathfinding and targeting in the developing eye appear normal. (Dworak et al., 2001)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hbs^Scer\UAS.cDa has cell adhesion defective \| somatic clone phenotype, enhanceable by sns^Scer\UAS.cGa/Scer\GAL4^GMR.PF (Bao et al., 2010) Scer\GAL4^hs.2sev, hbs^Scer\UAS.cDa has planar polarity defective phenotype, enhanceable by Psn¹⁴³/Psn[+] (Singh and Mlodzik, 2012)
Enhancer of
Statement Reference hbs^Scer\UAS.cDa/Scer\GAL4^GMR.PF is an enhancer of cell adhesion defective \| somatic clone phenotype of Scer\GAL4^GMR.PF, sns^Scer\UAS.cGa (Bao et al., 2010)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hbs^Scer\UAS.cDa has eye \| somatic clone phenotype, enhanceable by sns^Scer\UAS.cGa/Scer\GAL4^GMR.PF (Bao et al., 2010) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hbs^Scer\UAS.cDa has inter-ommatidial cell \| somatic clone phenotype, enhanceable by sns^Scer\UAS.cGa/Scer\GAL4^GMR.PF (Bao et al., 2010) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hbs^Scer\UAS.cDa has inter-ommatidial precursor cell \| somatic clone phenotype, enhanceable by sns^Scer\UAS.cGa/Scer\GAL4^GMR.PF (Bao et al., 2010) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hbs^Scer\UAS.cDa has ommatidium \| somatic clone phenotype, enhanceable by sns^Scer\UAS.cGa/Scer\GAL4^GMR.PF (Bao et al., 2010) Scer\GAL4^hs.2sev, hbs^Scer\UAS.cDa has eye phenotype, enhanceable by Psn¹⁴³/Psn[+] (Singh and Mlodzik, 2012)
Enhancer of
Statement Reference hbs^Scer\UAS.cDa/Scer\GAL4^GMR.PF is an enhancer of eye \| somatic clone phenotype of Scer\GAL4^GMR.PF, sns^Scer\UAS.cGa (Bao et al., 2010) hbs^Scer\UAS.cDa/Scer\GAL4^GMR.PF is an enhancer of inter-ommatidial cell \| somatic clone phenotype of Scer\GAL4^GMR.PF, sns^Scer\UAS.cGa (Bao et al., 2010) hbs^Scer\UAS.cDa/Scer\GAL4^GMR.PF is an enhancer of inter-ommatidial precursor cell \| somatic clone phenotype of Scer\GAL4^GMR.PF, sns^Scer\UAS.cGa (Bao et al., 2010) hbs^Scer\UAS.cDa/Scer\GAL4^GMR.PF is an enhancer of ommatidium \| somatic clone phenotype of Scer\GAL4^GMR.PF, sns^Scer\UAS.cGa (Bao et al., 2010)
Additional Comments
Genetic Interactions
Statement Reference Psn[143]/+ enhances the Scer\GAL4[hs.2sev]>hbs[Scer\UAS.cDa]-induced planar cell polarity (PCP) phenotype in the eye. (Singh and Mlodzik, 2012) Clonal expression of both hbs[Scer\UAS.cDa] and sns[Scer\UAS.cGa] within a single inter-ommatidial cell leads to a newly emergent, striking phenotype: 41% of hbs/sns-targeted inter-ommatidial cells are fully separated from ommatidia with neighboring inter-ommatidial cells intercalated around all sides of the cell. Surrounding inter-ommatidial cells maintain small interfaces with each other but larger interfaces with the hbs/sns-targeted cell. (Bao et al., 2010)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	41797 w^*; P{UAS-hbs.D}7.1
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	hbs^Scer\UAS.cDa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Dworak (Dworak et al., 2001)
Secondary FlyBase IDs

References ( 5 )
Research paper	Singh and Mlodzik, 2012, Dev. Cell 23(1): 82--96 Hibris, a Drosophila Nephrin Homolog, Is Required for Presenilin-Mediated Notch and APP-like Cleavages. [FBrf0218978] Bao et al., 2010, Dev. Biol. 344(2): 948--956 Preferential adhesion maintains separation of ommatidia in the Drosophila eye. [FBrf0211429] Bao and Cagan, 2005, Dev. Cell 8(6): 925--935 Preferential adhesion mediated by Hibris and Roughest regulates morphogenesis and patterning in the Drosophila eye. [FBrf0187402] Dworak et al., 2001, Development 128(21): 4265--4276 Characterization of Drosophila hibris, a gene related to human nephrin. [FBrf0139639]
Personal communication to FlyBase	Weber, 2012.9.7, P{UAS-hbs.HA} insertion. P{UAS-hbs.HA} insertion. [FBrf0219471]

Allele Dmel\hbsScer\UAS.cDa

Allele Dmel\hbs^Scer\UAS.cDa