FlyBase Allele Report: Dmel\gro[UAS.cAa]

General Information

Symbol

Dmel\gro^UAS.cAa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Apidianakis

FlyBase ID

FBal0127977

Feature type

allele

Associated gene

Dmel\gro

Associated Insertion(s)

Carried in Construct

P{UAS-gro.A}

Also Known As

UAS-gro

Key Links

Transgenic product class

cDNA

(Apidianakis et al., 2001)

wild_type

(Apidianakis et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Apidianakis et al., 2001)

wild_type

(Apidianakis et al., 2001)

Mutagen

in vitro construct

(Apidianakis et al., 2001)

Progenitor genotype

Carried in construct

P{UAS-gro.A}

Cytology

Description

UASt sequences drive expression of full length gro cDNA.

(Apidianakis et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

gro

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^ey.PH

(Lee et al., 2009)

visible, with Scer\GAL4^ey.PU

(Abed et al., 2011)

Phenotype Manifest In

eye, with Scer\GAL4^ey.PH

(Lee et al., 2009)

eye, with Scer\GAL4^ey.PU

(Abed et al., 2011)

mesothoracic tergum & macrochaeta, with Scer\GAL4^sca-C253

(Hasson et al., 2005)

sensory mother cell, with Scer\GAL4^sca-C253

(Hasson et al., 2005)

ventral nerve cord primordium, with Scer\GAL4^rho.PU

(Orian et al., 2007)

Detailed Description

Statement

Reference

Expression of gro^Scer\UAS.cAa under the control of Scer\GAL4^ey.PU results in flies with small, deformed eyes.

(Abed et al., 2011)

Expression of gro^Scer\UAS.cAa under the control of Scer\GAL4^ey.PH inhibits eye development.

(Lee et al., 2009)

Stage 9 embryos expressing gro^Scer\UAS.cAa under the control of Scer\GAL4^rho.PU show neuroblast hypoplasia and reduced mitotic activity in the developing neuroectoderm.

(Orian et al., 2007)

Expression of gro^Scer\UAS.cAa under the control of Scer\GAL4^sca-C253 leads to bristle loss on the notum, following suppression of sensory organ precursor cells.

(Hasson et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^ey.PU, gro^UAS.cAa has visible phenotype, suppressible by dgrn^UASp.cBa/Scer\GAL4^ey.PU

(Abed et al., 2011)

Scer\GAL4^ey.PU, gro^UAS.cAa has visible phenotype, suppressible | partially by dgrn^I268A.UASp/Scer\GAL4^ey.PU

(Abed et al., 2011)

Scer\GAL4^ey.PH, gro^UAS.cAa has visible phenotype, suppressible | partially by Hipk^UAS.Tag:HA, Scer\GAL4^ey.PH

(Lee et al., 2009)

NOT suppressed by

Statement

Reference

Scer\GAL4^ey.PU, gro^UAS.cAa has visible phenotype, non-suppressible by dgrn^HCAA.UASp/Scer\GAL4^ey.PU

(Abed et al., 2011)

Scer\GAL4^ey.PU, gro^UAS.cAa has visible phenotype, non-suppressible by dgrn^ΔSIMs.UASp/Scer\GAL4^ey.PU

(Abed et al., 2011)

Enhancer of

Statement

Reference

Scer\GAL4^69B/gro^UAS.cAa is an enhancer of partially lethal - majority die | embryonic stage phenotype of Scer\GAL4^69B, mid^UAS.Tag:FLAG

(Formaz-Preston et al., 2012)

gro^UAS.cAa, Scer\GAL4^ey.PH is an enhancer of visible phenotype of H^UAS.cGa, Scer\GAL4^ey.PH

(Lee et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^Bx-MS1096, gro^UAS.cAa has wing vein phenotype, enhanceable by E(spl)mβ-HLH^UAS.cdCa, Scer\GAL4^Bx-MS1096

(Hasson et al., 2005)

Suppressed by

Statement

Reference

Scer\GAL4^ey.PU, gro^UAS.cAa has eye phenotype, suppressible by dgrn^UASp.cBa/Scer\GAL4^ey.PU

(Abed et al., 2011)

Scer\GAL4^ey.PU, gro^UAS.cAa has eye phenotype, suppressible | partially by dgrn^I268A.UASp/Scer\GAL4^ey.PU

(Abed et al., 2011)

Scer\GAL4^ey.PH, gro^UAS.cAa has eye phenotype, suppressible | partially by Hipk^UAS.Tag:HA, Scer\GAL4^ey.PH

(Lee et al., 2009)

Scer\GAL4^sca-C253, gro^UAS.cAa has mesothoracic tergum & macrochaeta phenotype, suppressible by Scer\GAL4^sca-C253/btl::Egfr^λ.UAS

(Hasson et al., 2005)

NOT suppressed by

Statement

Reference

Scer\GAL4^ey.PU, gro^UAS.cAa has eye phenotype, non-suppressible by dgrn^HCAA.UASp/Scer\GAL4^ey.PU

(Abed et al., 2011)

Scer\GAL4^ey.PU, gro^UAS.cAa has eye phenotype, non-suppressible by dgrn^ΔSIMs.UASp/Scer\GAL4^ey.PU

(Abed et al., 2011)

Enhancer of

Statement

Reference

Scer\GAL4^69B/gro^UAS.cAa is an enhancer of ventral denticle belt phenotype of Scer\GAL4^69B, mid^UAS.Tag:FLAG

(Formaz-Preston et al., 2012)

gro^UAS.cAa, Scer\GAL4^ey.PH is an enhancer of eye phenotype of H^UAS.cGa, Scer\GAL4^ey.PH

(Lee et al., 2009)

NOT Suppressor of

Statement

Reference

Scer\GAL4^Bx-MS1096/gro^UAS.cAa is a non-suppressor of wing vein phenotype of Scer\GAL4^Bx-MS1096, rl^Sem.cUa.UAS

(Hasson et al., 2005)

Other

Statement

Reference

H^UAS.cGa, Scer\GAL4^ey.PH, gro^UAS.cAa has adult head phenotype

(Lee et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of gro^Scer\UAS.cAa does not enhance the penetrance of the embryonic lethality phenotype caused by expression of mid^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^69B. However, the fraction of dead embryos which show denticle belt fusions is increased by 33% to 58%.

Co-expression of gro^Scer\UAS.cAa and mid^{F41E.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^69B does not result in denticle belt fusions.

(Formaz-Preston et al., 2012)

Co-expression of dgrn^{Scer\UAS.P\T.cBa} suppresses the small, deformed eye phenotype caused by expression of gro^Scer\UAS.cAa under the control of Scer\GAL4^ey.PU.

Co-expression of either dgrn^{HCAA.Scer\UAS.P\T} or dgrn^{ΔSIMs.Scer\UAS.P\T} does not suppress the small, deformed eye phenotype caused by expression of gro^Scer\UAS.cAa under the control of Scer\GAL4^ey.PU.

Co-expression of dgrn^{I268A.Scer\UAS.P\T} slightly suppresses the small, deformed eye phenotype caused by expression of gro^Scer\UAS.cAa under the control of Scer\GAL4^ey.PU.

(Abed et al., 2011)

Co-expression of hipk^{Scer\UAS.T:Ivir\HA1} partially suppresses the eye loss phenotype caused by expression of gro^Scer\UAS.cAa under the control of Scer\GAL4^ey.PH.

(Lee et al., 2009)

Coexpression of HLHmβ^{Scer\UAS.cdCa} and gro^Scer\UAS.cAa, under the control of Scer\GAL4^Bx-MS1096, leads to a greater loss of wing veins than expression of HLHmβ^{Scer\UAS.cdCa} alone. Coexpression of gro^Scer\UAS.cAa with rl^Sem.Scer\UAS, under the control of Scer\GAL4^Bx-MS1096, is unable to suppress the thick wing vein phenotype seen when only rl^Sem.Scer\UAS is expressed. Coexpression of btl::Egfr^{Scer\UAS.T:λ\cI-DD} with gro^Scer\UAS.cAa, under the control of Scer\GAL4^sca-C253, suppresses the bristle-loss phenotype on the notum seen when gro^Scer\UAS.cAa alone is expressed (driven by Scer\GAL4^sca-C253).

(Hasson et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Carried in a plasmid and transfected into S2 cells.

(Apidianakis et al., 2001)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

gro^Scer\UAS.cAa

gro^UAS.cAa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Apidianakis

(Apidianakis et al., 2001)

Secondary FlyBase IDs

References (9)

Report Sections

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