Allele Dmel\gro^Scer\UAS.cAa

General Information
Symbol	Dmel\gro^Scer\UAS.cAa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Apidianakis	FlyBase ID	FBal0127977
Feature type	allele	Associated gene	Dmel\gro

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Apidianakis et al., 2001)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS sequences drive expression of full length gro cDNA. (Apidianakis et al., 2001)
Carried in construct	P{UAS-gro.A} (Apidianakis et al., 2001, Hasson et al., 2001, Lee et al., 2009, Hasson et al., 2005, Abed et al., 2011, Orian et al., 2007, Helman et al., 2011, Formaz-Preston et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	visible, with Scer\GAL4^ey.PH (Lee et al., 2009) visible, with Scer\GAL4^ey.PU (Abed et al., 2011)
Phenotype Manifest In
	eye, with Scer\GAL4^ey.PH (Lee et al., 2009) eye, with Scer\GAL4^ey.PU (Abed et al., 2011) mesothoracic tergum & macrochaeta, with Scer\GAL4^sca-C253 (Hasson et al., 2005) sensory mother cell, with Scer\GAL4^sca-C253 (Hasson et al., 2005) ventral nerve cord primordium, with Scer\GAL4^rho.PU (Orian et al., 2007)
Detailed Description
	Statement Reference Expression of gro[Scer\UAS.cAa] under the control of Scer\GAL4[ey.PU] results in flies with small, deformed eyes. (Abed et al., 2011) Expression of gro[Scer\UAS.cAa] under the control of Scer\GAL4[ey.PH] inhibits eye development. (Lee et al., 2009) Stage 9 embryos expressing gro[Scer\UAS.cAa] under the control of Scer\GAL4[rho.PU] show neuroblast hypoplasia and reduced mitotic activity in the developing neuroectoderm. (Orian et al., 2007) Expression of gro^Scer\UAS.cAa under the control of Scer\GAL4^sca-C253 leads to bristle loss on the notum, following suppression of sensory organ precursor cells. (Hasson et al., 2005)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Scer\GAL4^ey.PH, gro^Scer\UAS.cAa has visible phenotype, suppressible \| partially by hipk^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^ey.PH (Lee et al., 2009) Scer\GAL4^ey.PU, gro^Scer\UAS.cAa has visible phenotype, suppressible \| partially by dgrn^{I268A.Scer\UAS.P\T}/Scer\GAL4^ey.PU (Abed et al., 2011) Scer\GAL4^ey.PU, gro^Scer\UAS.cAa has visible phenotype, suppressible by dgrn^{Scer\UAS.P\T.cBa}/Scer\GAL4^ey.PU (Abed et al., 2011)
NOT suppressed by
Statement Reference Scer\GAL4^ey.PU, gro^Scer\UAS.cAa has visible phenotype, non-suppressible by dgrn^{HCAA.Scer\UAS.P\T}/Scer\GAL4^ey.PU (Abed et al., 2011) Scer\GAL4^ey.PU, gro^Scer\UAS.cAa has visible phenotype, non-suppressible by dgrn^{ΔSIMs.Scer\UAS.P\T}/Scer\GAL4^ey.PU (Abed et al., 2011)
Enhancer of
Statement Reference gro^Scer\UAS.cAa, Scer\GAL4^ey.PH is an enhancer of visible phenotype of H^Scer\UAS.cGa, Scer\GAL4^ey.PH (Lee et al., 2009) Scer\GAL4^69B/gro^Scer\UAS.cAa is an enhancer of partially lethal - majority die \| embryonic stage phenotype of Scer\GAL4^69B, mid^{Scer\UAS.T:Zzzz\FLAG} (Formaz-Preston et al., 2012)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^Bx-MS1096, gro^Scer\UAS.cAa has wing vein phenotype, enhanceable by E(spl)mβ-HLH^{Scer\UAS.cdCa}, Scer\GAL4^Bx-MS1096 (Hasson et al., 2005)
Suppressed by
Statement Reference Scer\GAL4^ey.PH, gro^Scer\UAS.cAa has eye phenotype, suppressible \| partially by hipk^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^ey.PH (Lee et al., 2009) Scer\GAL4^ey.PU, gro^Scer\UAS.cAa has eye phenotype, suppressible \| partially by dgrn^{I268A.Scer\UAS.P\T}/Scer\GAL4^ey.PU (Abed et al., 2011) Scer\GAL4^ey.PU, gro^Scer\UAS.cAa has eye phenotype, suppressible by dgrn^{Scer\UAS.P\T.cBa}/Scer\GAL4^ey.PU (Abed et al., 2011) Scer\GAL4^sca-C253, gro^Scer\UAS.cAa has mesothoracic tergum & macrochaeta phenotype, suppressible by Scer\GAL4^sca-C253/btl::Egfr^{Scer\UAS.T:λ\cI-DD} (Hasson et al., 2005)
NOT suppressed by
Statement Reference Scer\GAL4^ey.PU, gro^Scer\UAS.cAa has eye phenotype, non-suppressible by dgrn^{HCAA.Scer\UAS.P\T}/Scer\GAL4^ey.PU (Abed et al., 2011) Scer\GAL4^ey.PU, gro^Scer\UAS.cAa has eye phenotype, non-suppressible by dgrn^{ΔSIMs.Scer\UAS.P\T}/Scer\GAL4^ey.PU (Abed et al., 2011)
Enhancer of
Statement Reference gro^Scer\UAS.cAa, Scer\GAL4^ey.PH is an enhancer of eye phenotype of H^Scer\UAS.cGa, Scer\GAL4^ey.PH (Lee et al., 2009) Scer\GAL4^69B/gro^Scer\UAS.cAa is an enhancer of denticle belt phenotype of Scer\GAL4^69B, mid^{Scer\UAS.T:Zzzz\FLAG} (Formaz-Preston et al., 2012)
NOT Suppressor of
Statement Reference Scer\GAL4^Bx-MS1096/gro^Scer\UAS.cAa is a non-suppressor of wing vein phenotype of Scer\GAL4^Bx-MS1096, rl^Sem.Scer\UAS (Hasson et al., 2005)
Other
Statement Reference H^Scer\UAS.cGa, Scer\GAL4^ey.PH, gro^Scer\UAS.cAa has adult head phenotype (Lee et al., 2009)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of gro[Scer\UAS.cAa] does not enhance the penetrance of the embryonic lethality phenotype caused by expression of mid[Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[69B]. However, the fraction of dead embryos which show denticle belt fusions is increased by 33% to 58%. Co-expression of gro[Scer\UAS.cAa] and mid[F41E.Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[69B] does not result in denticle belt fusions. (Formaz-Preston et al., 2012) Co-expression of dgrn[Scer\UAS.P\T.cBa] suppresses the small, deformed eye phenotype caused by expression of gro[Scer\UAS.cAa] under the control of Scer\GAL4[ey.PU]. Co-expression of either dgrn[HCAA.Scer\UAS.P\T] or dgrn[ΔSIMs.Scer\UAS.P\T] does not suppress the small, deformed eye phenotype caused by expression of gro[Scer\UAS.cAa] under the control of Scer\GAL4[ey.PU]. Co-expression of dgrn[I268A.Scer\UAS.P\T] slightly suppresses the small, deformed eye phenotype caused by expression of gro[Scer\UAS.cAa] under the control of Scer\GAL4[ey.PU]. (Abed et al., 2011) Co-expression of hipk[Scer\UAS.T:Ivir\HA1] partially suppresses the eye loss phenotype caused by expression of gro[Scer\UAS.cAa] under the control of Scer\GAL4[ey.PH]. (Lee et al., 2009) Coexpression of HLHmβ^{Scer\UAS.cdCa} and gro^Scer\UAS.cAa, under the control of Scer\GAL4^Bx-MS1096, leads to a greater loss of wing veins than expression of HLHmβ^{Scer\UAS.cdCa} alone. Coexpression of gro^Scer\UAS.cAa with rl^Sem.Scer\UAS, under the control of Scer\GAL4^Bx-MS1096, is unable to suppress the thick wing vein phenotype seen when only rl^Sem.Scer\UAS is expressed. Coexpression of btl::Egfr^{Scer\UAS.T:λ\cI-DD} with gro^Scer\UAS.cAa, under the control of Scer\GAL4^sca-C253, suppresses the bristle-loss phenotype on the notum seen when gro^Scer\UAS.cAa alone is expressed (driven by Scer\GAL4^sca-C253). (Hasson et al., 2005)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Carried in a plasmid and transfected into S2 cells. (Apidianakis et al., 2001)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	gro^Scer\UAS.cAa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Apidianakis (Apidianakis et al., 2001)
Secondary FlyBase IDs

References ( 8 )
Research paper	Formaz-Preston et al., 2012, Dev. Biol. 369(2): 319--329 The Tbx20 homolog Midline represses wingless in conjunction with Groucho during the maintenance of segment polarity. [FBrf0219199] Abed et al., 2011, EMBO J. 30(7): 1289--1301 Degringolade, a SUMO-targeted ubiquitin ligase, inhibits Hairy/Groucho-mediated repression. [FBrf0213366] Helman et al., 2011, Curr. Biol. 21(13): 1102--1110 Phosphorylation of Groucho Mediates RTK Feedback Inhibition and Prolonged Pathway Target Gene Expression. [FBrf0214255] Lee et al., 2009, Dev. Biol. 325(1): 263--272 Hipk is an essential protein that promotes Notch signal transduction in the Drosophila eye by inhibition of the global co-repressor Groucho. [FBrf0206915] Orian et al., 2007, Proc. Natl. Acad. Sci. U.S.A. 104(40): 15771--15776 A Myc-Groucho complex integrates EGF and Notch signaling to regulate neural development. [FBrf0200831] Hasson et al., 2005, Nat. Genet. 37(1): 101--105 EGFR signaling attenuates Groucho-dependent repression to antagonize Notch transcriptional output. [FBrf0184190] Apidianakis et al., 2001, Development 128(21): 4361--4370 Groucho mediates a Ci-independent mechanism of hedgehog repression in the anterior wing pouch. [FBrf0139641] Hasson et al., 2001, EMBO J. 20(20): 5725--5736 Brinker requires two corepressors for maximal and versatile repression in Dpp signalling. [FBrf0139645]

Allele Dmel\groScer\UAS.cAa

Allele Dmel\gro^Scer\UAS.cAa