|Feature type||allele||Associated gene||Dmel\d|
|Allele class||loss of function allele|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
Contains an 11bp deletion, predicted to cause a truncation of the expressed product at R83 near the N terminus of the head domain, causing a frameshift closely followed by a stop codon.
11bp deletion in the myosin head domain, resulting in a frameshift and stop codon.
|Phenotype Manifest In|
d[GC13]/d wing discs irradiated with 40Gy gamma-irradiation display a reduced regenerative capacity compared to un-irradiated controls.
Mutants have small wings.
d[GC13]/Df(2L)ED623 flies show planar cell polarity (PCP) defects in the wing. The animals also have abdominal PCP defects; polarity is almost normal in the anterior compartment, abnormal near the anterior/posterior compartment boundary and reversed in the posterior compartment.
The legs of dGC13 pharate adults are shorter than wild-type legs; the intermediate and distal segments of the leg (femur through tarsus) are noticeably shortened, and some tarsal segments are fused, typically resulting in the formation of only three tarsal segments instead of the normal five. In some cases no external leg tissue is evident, or the leg appears to form a single mass of tissue. Pupal legs, however, are consistently shortened but never absent or severely deformed. Examination of pupae shows that leg absence results from a failure of disc eversion. The legs of dGC13/Df(2L)ED623 and dGC13/d210 flies are smaller than wild-type legs. dGC13 mutant clones in the leg cause reduced growth and fusion of tarsal segments, but do not cause disc eversion failure. The wings of dGC13 adults are small compared to wild type and exhibit abnormal wing patterning, as evidenced by a variable phenotype that includes extra, missing and mis-positioned crossveins. Extra or missing crossveins are often observed in adult flies with dGC13 clones in the wing, and with d1/dGC13 animals. The wings of dGC13/Df(2L)N22-5 are smaller than wild type while the wings of dGC13/Df(2L)ED623 are relatively normal. dGC13 flies show a mild polarity phenotype. As in wild-type animals, hairs in the abdomen point posteriorly, most leg bristles and wing hairs point distally and most ommatidia are correctly oriented. In dGC13 mutant clones in the abdomen, hair polarity appears normal. dGC13 clones in the eye show some disorganization of ommatidial orientation.
|NOT Suppressor of|
|Phenotype Manifest In|
|NOT Suppressor of|
Scer\GAL4[nub-AC-62]-mediated expression of Zyx102EF[NIG.32018R] (together with Dcr-2[Scer\UAS.cDa]) enhances the small wing phenotype of d[GC13] flies.
d[GC13] does not suppress the wing disc overgrowth seen in wts[P1] mutant larvae. d[GC13] does not suppress the wing disc overgrowth seen in ex[e1] mutant larvae.
ft8, dGC13 double mutant clones in the leg do not induce outgrowths or the appearance of vesicles in the cuticular tissue, a phenotype seen when ft8 single mutant clones are generated in the leg. dGC13 partially suppresses the polarity phenotype of ft8. This is evidenced by comparison of ft8 and dGC13 single mutant clones with ft8, dGC13 double mutant clones in the abdomen. While all (39/39) ft8 clones have a polarity phenotype, only 54% of ft8, dGC13 clones show this phenotype (vs 3% of dGC13 clones). The ft8 single mutant clones exhibit some hairs at an angle greater than 90o relative to the normal orientation, while only 26% of ft8, dGC13 clones had hairs of this angle. The polarity phenotype of ft8, dGC13 double mutant clones in the eye is similar to that of ft8 single mutant clones (28 vs 32 out of 33 clones including ommatidia rotated more than 90o away from the normal position, respectively. The polarity of hairs surrounding ck13, dGC13 clones in the abdomen appear normal.
|Complementation & Rescue Data|
|Partially rescued by|
d[T:Avic\GFP] rescues the morphological defects and viability of d[GC13]/d animals. d[+tcBa] rescues the morphological defects and viability of d[GC13]/d animals.
|Stocks ( 2 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 3 )|
|Secondary FlyBase IDs|
|References ( 8 )|
|Personal communication to FlyBase|