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General Information
Symbol
Dmel\dGC13
Species
D. melanogaster
Name
FlyBase ID
FBal0128007
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
dachsGC13
Mutagen
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Contains an 11bp deletion, predicted to cause a truncation of the expressed product at R83 near the N terminus of the head domain, causing a frameshift closely followed by a stop codon.
11bp deletion in the myosin head domain, resulting in a frameshift and stop codon.
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
dGC13/d210 transheterozygotes display some hair orientation defects in the posterior dorsal abdomen, as compared to controls.
dGC13/dGC13 adult eyes (created by the EGUF method in otherwise heterozygous animals) are significantly smaller compared to wild-type. d1/dGC13 transheterozygote third instar larvae have smaller eye and wing discs compared to wild-type.
dGC13/d1 wing discs irradiated with 40Gy gamma-irradiation display a reduced regenerative capacity compared to un-irradiated controls.
Mutants have small wings.
dGC13/Df(2L)ED623 flies show planar cell polarity (PCP) defects in the wing. The animals also have abdominal PCP defects; polarity is almost normal in the anterior compartment, abnormal near the anterior/posterior compartment boundary and reversed in the posterior compartment.
The legs of dGC13 pharate adults are shorter than wild-type legs; the intermediate and distal segments of the leg (femur through tarsus) are noticeably shortened, and some tarsal segments are fused, typically resulting in the formation of only three tarsal segments instead of the normal five. In some cases no external leg tissue is evident, or the leg appears to form a single mass of tissue. Pupal legs, however, are consistently shortened but never absent or severely deformed. Examination of pupae shows that leg absence results from a failure of disc eversion. The legs of dGC13/Df(2L)ED623 and dGC13/d210 flies are smaller than wild-type legs. dGC13 mutant clones in the leg cause reduced growth and fusion of tarsal segments, but do not cause disc eversion failure. The wings of dGC13 adults are small compared to wild type and exhibit abnormal wing patterning, as evidenced by a variable phenotype that includes extra, missing and mis-positioned crossveins. Extra or missing crossveins are often observed in adult flies with dGC13 clones in the wing, and with d1/dGC13 animals. The wings of dGC13/Df(2L)N22-5 are smaller than wild type while the wings of dGC13/Df(2L)ED623 are relatively normal. dGC13 flies show a mild polarity phenotype. As in wild-type animals, hairs in the abdomen point posteriorly, most leg bristles and wing hairs point distally and most ommatidia are correctly oriented. In dGC13 mutant clones in the abdomen, hair polarity appears normal. dGC13 clones in the eye show some disorganization of ommatidial orientation.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
NOT Enhancer of
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Enhanced by
NOT Enhancer of
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference
The small eye phenotype characteristic for dGC13/dGC13 adult eyes (created by the EGUF method in otherwise heterozygous animals) can be enhanced by combination with ZyxΔ41/ZyxΔ41. The reduced size of both eye and wing discs characteristic for d1/dGC13 transheterozygote third instar larvae is further enhanced by combination with ZyxΔ41/ZyxΔ41.
dGC13 suppresses the retinal neurodegeneration seen in ft8 clones. The photoreceptor degeneration caused by Gug75QN.Scer\UAS under the control of Scer\GAL4ninaE.PT is significantly suppressed inside dGC13 clones.
Scer\GAL4nub-AC-62-mediated expression of Zyx102EFNIG.32018R (together with Dcr-2Scer\UAS.cDa) enhances the small wing phenotype of dGC13 flies.
dGC13 does not suppress the wing disc overgrowth seen in wtsP1 mutant larvae. dGC13 does not suppress the wing disc overgrowth seen in exe1 mutant larvae.
ft8, dGC13 double mutant clones in the leg do not induce outgrowths or the appearance of vesicles in the cuticular tissue, a phenotype seen when ft8 single mutant clones are generated in the leg. dGC13 partially suppresses the polarity phenotype of ft8. This is evidenced by comparison of ft8 and dGC13 single mutant clones with ft8, dGC13 double mutant clones in the abdomen. While all (39/39) ft8 clones have a polarity phenotype, only 54% of ft8, dGC13 clones show this phenotype (vs 3% of dGC13 clones). The ft8 single mutant clones exhibit some hairs at an angle greater than 90o relative to the normal orientation, while only 26% of ft8, dGC13 clones had hairs of this angle. The polarity phenotype of ft8, dGC13 double mutant clones in the eye is similar to that of ft8 single mutant clones (28 vs 32 out of 33 clones including ommatidia rotated more than 90o away from the normal position, respectively. The polarity of hairs surrounding ck13, dGC13 clones in the abdomen appear normal.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments
Expression of either dScer\UAS.T:SV5\V5,T:Zzzz\His6 or dΔN.Scer\UAS.T:SV5\V5 under the control of Scer\GAL4tub.PU restores the viability of dGC13 mutant flies, while expresion of any of the following: dΔMH.Scer\UAS.T:SV5\V5, dΔ1C.Scer\UAS.T:SV5\V5, dΔ2C.Scer\UAS.T:SV5\V5, or dΔ3C.Scer\UAS.T:SV5\V5 does not rescue dGC13 viability.
dT:Avic\GFP rescues the morphological defects and viability of dGC13/d210 animals. d+tcBa rescues the morphological defects and viability of dGC13/d210 animals.
Expression of dScer\UAS.T:SV5\V5,T:Zzzz\His6 under the control of Scer\GAL4tub provides significant rescue of the wing and leg phenotypes of dGC13 mutants.
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
References (17)