Allele Dmel\d^GC13

General Information
Symbol	Dmel\d^GC13	Species	D. melanogaster
Name		FlyBase ID	FBal0128007
Feature type	allele	Associated gene	Dmel\d

Allele class	loss of function allele
Mutagen	gamma ray
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	loss of function allele (Katz, 2001.12.11, Mao et al., 2006)
Mutagen	gamma ray (Katz, 2001.12.11)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Contains an 11bp deletion, predicted to cause a truncation of the expressed product at R83 near the N terminus of the head domain, causing a frameshift closely followed by a stop codon. (Mao et al., 2006) 11bp deletion in the myosin head domain, resulting in a frameshift and stop codon. (Katz, 2001.12.11)
Cytology
Phenotypic Data
Phenotypic Class
	cell polarity defective (Mao et al., 2006) lethal \| pharate adult stage (Mao et al., 2006) planar polarity defective (with Df(2L)ED623) (Matakatsu and Blair, 2008) size defective (Rauskolb et al., 2011) visible (Mao et al., 2006) visible (with d²¹⁰) (Mao et al., 2006) visible (with Df(2L)ED623) (Mao et al., 2006) visible (with Df(2L)N22-5) (Mao et al., 2006)
Phenotype Manifest In
	adult abdomen (with Df(2L)ED623) (Matakatsu and Blair, 2008) crossvein (Mao et al., 2006) crossvein (with d¹) (Mao et al., 2006) crossvein \| somatic clone (Mao et al., 2006) leg (Mao et al., 2006) leg (with d²¹⁰) (Mao et al., 2006) leg (with Df(2L)ED623) (Mao et al., 2006) leg \| somatic clone (Mao et al., 2006) leg sensillum (Mao et al., 2006) ommatidium (Mao et al., 2006) ommatidium \| somatic clone (Mao et al., 2006) tarsal segment (Mao et al., 2006) tarsal segment \| somatic clone (Mao et al., 2006) ventral thoracic disc (Mao et al., 2006) wing (Mao et al., 2006, Rauskolb et al., 2011) wing (with Df(2L)N22-5) (Mao et al., 2006) wing disc \| conditional (Grusche et al., 2011) wing hair (Mao et al., 2006) wing hair (with Df(2L)ED623) (Matakatsu and Blair, 2008)
Detailed Description
	Statement Reference d[GC13]/d[1] wing discs irradiated with 40Gy gamma-irradiation display a reduced regenerative capacity compared to un-irradiated controls. (Grusche et al., 2011) Mutants have small wings. (Rauskolb et al., 2011) d[GC13]/Df(2L)ED623 flies show planar cell polarity (PCP) defects in the wing. The animals also have abdominal PCP defects; polarity is almost normal in the anterior compartment, abnormal near the anterior/posterior compartment boundary and reversed in the posterior compartment. (Matakatsu and Blair, 2008) The legs of d^GC13 pharate adults are shorter than wild-type legs; the intermediate and distal segments of the leg (femur through tarsus) are noticeably shortened, and some tarsal segments are fused, typically resulting in the formation of only three tarsal segments instead of the normal five. In some cases no external leg tissue is evident, or the leg appears to form a single mass of tissue. Pupal legs, however, are consistently shortened but never absent or severely deformed. Examination of pupae shows that leg absence results from a failure of disc eversion. The legs of d^GC13/Df(2L)ED623 and d^GC13/d²¹⁰ flies are smaller than wild-type legs. d^GC13 mutant clones in the leg cause reduced growth and fusion of tarsal segments, but do not cause disc eversion failure. The wings of d^GC13 adults are small compared to wild type and exhibit abnormal wing patterning, as evidenced by a variable phenotype that includes extra, missing and mis-positioned crossveins. Extra or missing crossveins are often observed in adult flies with d^GC13 clones in the wing, and with d¹/d^GC13 animals. The wings of d^GC13/Df(2L)N22-5 are smaller than wild type while the wings of d^GC13/Df(2L)ED623 are relatively normal. d^GC13 flies show a mild polarity phenotype. As in wild-type animals, hairs in the abdomen point posteriorly, most leg bristles and wing hairs point distally and most ommatidia are correctly oriented. In d^GC13 mutant clones in the abdomen, hair polarity appears normal. d^GC13 clones in the eye show some disorganization of ommatidial orientation. (Mao et al., 2006)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference d^GC13 has size defective phenotype, enhanceable by Zyx^NIG.32018R/Scer\GAL4^nub-AC-62 (Rauskolb et al., 2011)
Suppressor of
Statement Reference d^GC13 is a suppressor \| partially of cell polarity defective \| somatic clone phenotype of ft⁸ (Mao et al., 2006)
NOT Suppressor of
Statement Reference d^GC13 is a non-suppressor of increased cell number \| larval stage phenotype of ex^e1 (Cho et al., 2006)
Phenotype Manifest In
Enhanced by
Statement Reference d^GC13 has wing phenotype, enhanceable by Zyx^NIG.32018R/Scer\GAL4^nub-AC-62 (Rauskolb et al., 2011)
Suppressor of
Statement Reference d^GC13 is a suppressor \| partially of microchaeta \| somatic clone phenotype of ft⁸ (Mao et al., 2006) d^GC13 is a suppressor of leg \| somatic clone phenotype of ft⁸ (Mao et al., 2006)
NOT Suppressor of
Statement Reference d^GC13 is a non-suppressor of ommatidium \| somatic clone phenotype of ft⁸ (Mao et al., 2006) d^GC13 is a non-suppressor of wing disc \| larval stage phenotype of ex^e1 (Cho et al., 2006)
Additional Comments
Genetic Interactions
Statement Reference Scer\GAL4[nub-AC-62]-mediated expression of Zyx102EF[NIG.32018R] (together with Dcr-2[Scer\UAS.cDa]) enhances the small wing phenotype of d[GC13] flies. (Rauskolb et al., 2011) d[GC13] does not suppress the wing disc overgrowth seen in wts[P1] mutant larvae. d[GC13] does not suppress the wing disc overgrowth seen in ex[e1] mutant larvae. (Cho et al., 2006) ft⁸, d^GC13 double mutant clones in the leg do not induce outgrowths or the appearance of vesicles in the cuticular tissue, a phenotype seen when ft⁸ single mutant clones are generated in the leg. d^GC13 partially suppresses the polarity phenotype of ft⁸. This is evidenced by comparison of ft⁸ and d^GC13 single mutant clones with ft⁸, d^GC13 double mutant clones in the abdomen. While all (39/39) ft⁸ clones have a polarity phenotype, only 54% of ft⁸, d^GC13 clones show this phenotype (vs 3% of d^GC13 clones). The ft⁸ single mutant clones exhibit some hairs at an angle greater than 90^o relative to the normal orientation, while only 26% of ft⁸, d^GC13 clones had hairs of this angle. The polarity phenotype of ft⁸, d^GC13 double mutant clones in the eye is similar to that of ft⁸ single mutant clones (28 vs 32 out of 33 clones including ommatidia rotated more than 90^o away from the normal position, respectively. The polarity of hairs surrounding ck¹³, d^GC13 clones in the abdomen appear normal. (Mao et al., 2006)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	d²¹⁰/d^GC13 is rescued by d^+tcBa (Bosveld et al., 2012) d²¹⁰/d^GC13 is rescued by d^T:Avic\GFP (Bosveld et al., 2012)
Partially rescued by	d^GC13 is partially rescued by d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6}/Scer\GAL4^tub.PU (Mao et al., 2006)
Comments	d[T:Avic\GFP] rescues the morphological defects and viability of d[GC13]/d[210] animals. d[+tcBa] rescues the morphological defects and viability of d[GC13]/d[210] animals. (Bosveld et al., 2012) Expression of d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} under the control of Scer\GAL4^tub provides significant rescue of the wing and leg phenotypes of d^GC13 mutants. (Mao et al., 2006)
Stocks ( 2 )
Bloomington	6389 w^; d^GC13 pr¹ cn¹/CyO 28289 w^; d^GC13 P{neoFRT}40A/CyO, P{ActGFP}JMR1
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	d^GC13 (Mao et al., 2006, Christensen and Cook, 2006.8.30, Matakatsu and Blair, 2008, Rauskolb et al., 2011) dachs^<up>GC13</up> (Bosveld et al., 2012) dachs^GC13 (Grusche et al., 2011)
Name Synonym	dachs^GC13 (Cho et al., 2006)
Secondary FlyBase IDs

References ( 8 )
Research paper	Grusche et al., 2011, Dev. Biol. 350(2): 255--266 The Salvador/Warts/Hippo pathway controls regenerative tissue growth in Drosophila melanogaster. [FBrf0212888] Rauskolb et al., 2011, PLoS Biol. 9(6): e1000624 Zyxin links fat signaling to the hippo pathway. [FBrf0213900] Matakatsu and Blair, 2008, Curr. Biol. 18(18): 1390--1395 The DHHC palmitoyltransferase approximated regulates Fat signaling and Dachs localization and activity. [FBrf0206015] Cho et al., 2006, Nat. Genet. 38(10): 1142--1150 Delineation of a Fat tumor suppressor pathway. [FBrf0193283] Mao et al., 2006, Development 133(13): 2539--2551 Dachs: an unconventional myosin that functions downstream of Fat to regulate growth, affinity and gene expression in Drosophila. [FBrf0193425]
Supplementary material	Bosveld et al., 2012, Science 336(6082): Supporting Online Material (SOM): Materials and methods. [FBrf0218770]
Personal communication to FlyBase	Christensen and Cook, 2006.8.30, Isolation and characterization of Df(2L)BSC201. Isolation and characterization of Df(2L)BSC201. [FBrf0198983] Katz, 2001.12.11, fj and d alleles. fj and d alleles. [FBrf0141822]

Allele Dmel\dGC13

Allele Dmel\d^GC13