FlyBase Allele Report: Scer\GAL4[Hml.PG]

General Information

Symbol

Scer\GAL4^Hml.PG

Species

S. cerevisiae

Name

Hemolectin promoter construct of Goto

FlyBase ID

FBal0128083

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{Hml-GAL4.G}

Also Known As

Hml-GAL4, Hemolectin-GAL4

Allele class

Mutagen

in vitro construct - regulatory fusion

Nature of the Allele

Allele class

Mutagen

in vitro construct - regulatory fusion

(Goto et al., 2003, Goto, 2001.12.6)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{Hml-GAL4.G}

Cytology

Nature of the lesion

Statement

Reference

The P{Hml-GAL4.G} construct contains sequences from upstream of Hml driving GAL4 expression (represented by the Scer\GAL4^Hml.PG allele). This genomic fragment from upstream of Hml also contains the complete open reading frame of Tsp68C (represented by the Tsp68C^+t3.0 allele).

(Sinenko and Mathey-Prevot, 2004)

The 3.0kb upstream promoter region of Hml regulates expression of a GAL4 driver.

(Goto et al., 2003, Goto, 2001.12.6)

Allele components

Product class / Tool use(s)

Regulatory region(s)

Hml

Encoded product / tool

GAL4

binary expression system - driver

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 17 -- prepupal stage P1

hemocyte

(Goto et al., 2003)

larval stage

hemocyte

(Narita et al., 2004, Goto, 2001.12.6)

embryonic/larval hemocyte

(Kester and Nambu, 2011)

larval stage -- adult stage

hemocyte

(Shia et al., 2009)

Additional Information

Statement

Reference

Drives expression in hemocytes at late embryonic and all post-embryonic stages.

(FlyBase Curators, 2017)

Scer\GAL4^Hml.PG drives expression in hemocytes at post-embryonic stages.

(Shia et al., 2009)

Marker for

hemocyte

(Narita et al., 2004, Goto et al., 2003, Goto, 2001.12.6)

Reflects expression of

Hml

(Shia et al., 2009, Narita et al., 2004, Goto et al., 2003, Goto, 2001.12.6)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell death, with Dark^{D1292A.UAS.Tag:polyHis,Tag:MYC}

(Akdemir et al., 2006)

abnormal cell death, with Dark^{UAS.Tag:polyHis,Tag:MYC}

(Akdemir et al., 2006)

abnormal feeding behavior, with egr^dsRNA.UAS

(Mabery and Schneider, 2010)

abnormal immune response, with Asam\aopP^UAS.Tag:MYC

(Jones et al., 2012)

abnormal immune response, with egr^UAS.cUa

(Jones et al., 2012)

abnormal immune response, with Glt^GD5017

(Arefin et al., 2014)

abnormal immune response, with Glt^KK110236

(Arefin et al., 2014)

abnormal immune response, with Hsap\CASP1^UAS.cSa

(Shia et al., 2009)

abnormal immune response, with lwr^JF01386

(Paddibhatla et al., 2010)

abnormal immune response, with PGRP-LC^a.UAS.EGFP

(Schmidt et al., 2008)

abnormal immune response, with PGRP-LC^{a.UAS.Tag:FLAG}

(Schmidt et al., 2008)

abnormal immune response, with PGRP-LC^{a-III.UAS.Tag:FLAG}

(Schmidt et al., 2008)

abnormal immune response, with PGRP-LC^{a-V.UAS.Tag:FLAG}

(Schmidt et al., 2008)

abnormal immune response, with PGRP-LC^{I.UAS.Tag:FLAG}

(Schmidt et al., 2008)

abnormal immune response, with PGRP-LC^{II.UAS.Tag:FLAG}

(Schmidt et al., 2008)

abnormal immune response, with PGRP-LC^x.UAS.EGFP

(Schmidt et al., 2008)

abnormal immune response, with PGRP-LC^{x.UAS.Tag:FLAG}

(Schmidt et al., 2008)

abnormal immune response, with PGRP-LC^{x-IV.UAS.Tag:FLAG}

(Schmidt et al., 2008)

abnormal immune response, with PGRP-LC^{x-VI.UAS.Tag:FLAG}

(Schmidt et al., 2008)

abnormal immune response, with SPE^{active.UAS.Tag:V5}

(Paddibhatla et al., 2010)

abnormal immune response, with TM9SF4^UAS.cBa

(Bergeret et al., 2008)

abnormal immune response, with Uba2^HM05055

(Paddibhatla et al., 2010)

abnormal immune response | larval stage, with Als2^GD12154

(Howell et al., 2012)

abnormal immune response | larval stage, with aPKC^KK100874

(Howell et al., 2012)

abnormal immune response | larval stage, with DCTN1-p150^GD1455

(Howell et al., 2012)

abnormal immune response | larval stage, with DCX-EMAP^GD1583

(Howell et al., 2012)

abnormal immune response | larval stage, with Dhc64C^GD12258

(Howell et al., 2012)

abnormal immune response | larval stage, with dia^KK101745

(Howell et al., 2012)

abnormal immune response | larval stage, with Eb1^GD13490

(Howell et al., 2012)

abnormal immune response | larval stage, with Pten^GD13500

(Howell et al., 2012)

abnormal immune response | larval stage, with Rac1^GD17411

(Howell et al., 2012)

abnormal immune response | larval stage, with Rac2^GD17536

(Howell et al., 2012)

abnormal immune response | larval stage, with RhoGEF3^GD7547

(Howell et al., 2012)

abnormal immune response | larval stage, with Shc^GD11053

(Howell et al., 2012)

abnormal immune response | larval stage, with Vav^GD1557

(Howell et al., 2012)

increased cell number, with Ras85D^V12.UAS

(Ashton-Beaucage et al., 2014)

increased cell number | larval stage, with Alk^{act.UAS.Tag:HA}

(Zettervall et al., 2004)

increased cell number | larval stage, with hep^CA.UAS

(Zettervall et al., 2004)

increased cell number | larval stage, with pan^ΔN.UAS

(Zettervall et al., 2004)

increased cell number | larval stage, with pnt^P1.UAS

(Zettervall et al., 2004)

increased cell number | larval stage, with Rac1^UAS.cLa

(Zettervall et al., 2004)

lethal - all die before end of pupal stage, with TM9SF4^UAS.cBa

(Bergeret et al., 2008)

lethal - all die before end of second instar larval stage, with vvl^UAS.cCa

(Junell et al., 2010)

lethal - all die during pupal stage, with Hsap\CASP1^UAS.cSa

(Shia et al., 2009)

melanotic mass phenotype | adult stage, with PGRP-LC^{I.UAS.Tag:FLAG}

(Schmidt et al., 2008)

melanotic mass phenotype | adult stage, with pzg^dsRNA.UAS

(Kugler et al., 2011)

melanotic mass phenotype | larval stage, with PGRP-LC^{I.UAS.Tag:FLAG}

(Schmidt et al., 2008)

melanotic mass phenotype | larval stage, with pzg^dsRNA.UAS

(Kugler et al., 2011)

melanotic mass phenotype | larval stage, with vvl^UAS.cCa

(Junell et al., 2010)

melanotic mass phenotype | pupal stage, with PGRP-LC^{I.UAS.Tag:FLAG}

(Schmidt et al., 2008)

melanotic mass phenotype | pupal stage, with pzg^dsRNA.UAS

(Kugler et al., 2011)

melanotic necrosis | larval stage, with chinmo^UAS.fl

(Flaherty et al., 2010)

short lived | conditional, with Glt^GD5017

(Arefin et al., 2014)

short lived | conditional, with Glt^KK110236

(Arefin et al., 2014)

some die during pupal stage, with TM9SF4^UAS.cBa

(Bergeret et al., 2008)

viable, with fon^UAS.EGFP

(Lindgren et al., 2008)

Phenotype Manifest In

embryonic/larval fat body, with lwr^JF01386

(Paddibhatla et al., 2010)

embryonic/larval fat body, with spz^act.UAS

(Paddibhatla et al., 2010)

embryonic/larval fat body, with Uba2^HM05055

(Paddibhatla et al., 2010)

embryonic/larval hemocoel, with lwr^JF01386

(Paddibhatla et al., 2010)

embryonic/larval hemocoel, with spz^act.UAS

(Paddibhatla et al., 2010)

embryonic/larval hemocoel, with Uba2^HM05055

(Paddibhatla et al., 2010)

embryonic/larval hemocyte, with Cele\ced-3^UAS.cSa

(Shia et al., 2009)

embryonic/larval hemocyte, with Dscam1^dsRNA.UAS

(Watson et al., 2005)

embryonic/larval hemocyte, with Hsap\CASP1^UAS.cSa

(Shia et al., 2009)

embryonic/larval lymph gland, with Hsap\CASP1^UAS.cSa

(Shia et al., 2009)

embryonic/larval plasmatocyte, with Asam\aopP^UAS.Tag:MYC

(Jones et al., 2012)

embryonic/larval plasmatocyte, with egr^UAS.cUa

(Jones et al., 2012)

hemocyte, with Alk^{act.UAS.Tag:HA}

(Zettervall et al., 2004)

hemocyte, with pan^ΔN.UAS

(Zettervall et al., 2004)

hemocyte, with pnt^P1.UAS

(Zettervall et al., 2004)

hemocyte, with Rac1^UAS.cLa

(Zettervall et al., 2004)

hemocyte, with Ras85D^V12.UAS

(Ashton-Beaucage et al., 2014)

hemocyte | larval stage, with hid^UAS.cZa

(Gafuik and Steller, 2011)

lamellocyte, with hep^CA.UAS

(Zettervall et al., 2004)

lamellocyte, with pan^ΔN.UAS

(Zettervall et al., 2004)

lamellocyte, with Rac1^UAS.cLa

(Zettervall et al., 2004)

melanotic mass, with hep^CA.UAS

(Zettervall et al., 2004)

melanotic mass, with pan^ΔN.UAS

(Zettervall et al., 2004)

melanotic mass, with pnt^P1.UAS

(Zettervall et al., 2004)

melanotic mass, with Rac1^UAS.cLa

(Zettervall et al., 2004)

melanotic mass | adult stage, with pzg^dsRNA.UAS

(Kugler et al., 2011)

melanotic mass | larval stage, with pzg^dsRNA.UAS

(Kugler et al., 2011)

melanotic mass | pupal stage, with pzg^dsRNA.UAS

(Kugler et al., 2011)

secondary lobe of embryonic/larval lymph gland | ectopic | third instar larval stage, with asrij^NIG.13533R

(Kulkarni et al., 2011)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Ras85D^V12.UAS, Scer\GAL4^Hml.PG has increased cell number phenotype, suppressible by Caper^NIG.11266R, Scer\GAL4^Hml.PG

(Ashton-Beaucage et al., 2014)

Ras85D^V12.UAS, Scer\GAL4^Hml.PG has increased cell number phenotype, suppressible by CG1603^GD9160, Scer\GAL4^Hml.PG

(Ashton-Beaucage et al., 2014)

Ras85D^V12.UAS, Scer\GAL4^Hml.PG has increased cell number phenotype, suppressible | partially by gfzf^GD10273, Scer\GAL4^Hml.PG

(Ashton-Beaucage et al., 2014)

Ras85D^V12.UAS, Scer\GAL4^Hml.PG has increased cell number phenotype, suppressible | partially by Prp19^GD11681, Scer\GAL4^Hml.PG

(Ashton-Beaucage et al., 2014)

Ras85D^V12.UAS, Scer\GAL4^Hml.PG has increased cell number phenotype, suppressible | partially by Prp19^KK101335, Scer\GAL4^Hml.PG

(Ashton-Beaucage et al., 2014)

Ras85D^V12.UAS, Scer\GAL4^Hml.PG has increased cell number phenotype, suppressible | partially by rl^GD4697, Scer\GAL4^Hml.PG

(Ashton-Beaucage et al., 2014)

Suppressor of

Statement

Reference

Caper^NIG.11266R, Scer\GAL4^Hml.PG is a suppressor of increased cell number phenotype of Ras85D^V12.UAS, Scer\GAL4^Hml.PG

(Ashton-Beaucage et al., 2014)

CG1603^GD9160, Scer\GAL4^Hml.PG is a suppressor of increased cell number phenotype of Ras85D^V12.UAS, Scer\GAL4^Hml.PG

(Ashton-Beaucage et al., 2014)

gfzf^GD10273, Scer\GAL4^Hml.PG is a suppressor | partially of increased cell number phenotype of Ras85D^V12.UAS, Scer\GAL4^Hml.PG

(Ashton-Beaucage et al., 2014)

Prp19^GD11681, Scer\GAL4^Hml.PG is a suppressor | partially of increased cell number phenotype of Ras85D^V12.UAS, Scer\GAL4^Hml.PG

(Ashton-Beaucage et al., 2014)

Prp19^KK101335, Scer\GAL4^Hml.PG is a suppressor | partially of increased cell number phenotype of Ras85D^V12.UAS, Scer\GAL4^Hml.PG

(Ashton-Beaucage et al., 2014)

rl^GD4697, Scer\GAL4^Hml.PG is a suppressor | partially of increased cell number phenotype of Ras85D^V12.UAS, Scer\GAL4^Hml.PG

(Ashton-Beaucage et al., 2014)

Scer\GAL4^Hml.PG/crq^UAS.cFa is a suppressor | partially of lethal phenotype of Trpml¹

(Venkatachalam et al., 2008)

Phenotype Manifest In

Suppressed by

Statement

Reference

Ras85D^V12.UAS, Scer\GAL4^Hml.PG has hemocyte phenotype, suppressible by Prp19^KK101335, Scer\GAL4^Hml.PG

(Ashton-Beaucage et al., 2014)

Ras85D^V12.UAS, Scer\GAL4^Hml.PG has hemocyte phenotype, suppressible by Prp19^GD11681, Scer\GAL4^Hml.PG

(Ashton-Beaucage et al., 2014)

Ras85D^V12.UAS, Scer\GAL4^Hml.PG has hemocyte phenotype, suppressible by Caper^NIG.11266R, Scer\GAL4^Hml.PG

(Ashton-Beaucage et al., 2014)

Ras85D^V12.UAS, Scer\GAL4^Hml.PG has hemocyte phenotype, suppressible by rl^GD4697, Scer\GAL4^Hml.PG

(Ashton-Beaucage et al., 2014)

Ras85D^V12.UAS, Scer\GAL4^Hml.PG has hemocyte phenotype, suppressible by CG1603^GD9160, Scer\GAL4^Hml.PG

(Ashton-Beaucage et al., 2014)

Ras85D^V12.UAS, Scer\GAL4^Hml.PG has hemocyte phenotype, suppressible by gfzf^GD10273, Scer\GAL4^Hml.PG

(Ashton-Beaucage et al., 2014)

Scer\GAL4^Hml.PG, hid^UAS.cZa has hemocyte | larval stage phenotype, suppressible | partially by Ras85D^R68Q

(Gafuik and Steller, 2011)

Suppressor of

Statement

Reference

Prp19^KK101335, Scer\GAL4^Hml.PG is a suppressor of hemocyte phenotype of Ras85D^V12.UAS, Scer\GAL4^Hml.PG

(Ashton-Beaucage et al., 2014)

Prp19^GD11681, Scer\GAL4^Hml.PG is a suppressor of hemocyte phenotype of Ras85D^V12.UAS, Scer\GAL4^Hml.PG

(Ashton-Beaucage et al., 2014)

Caper^NIG.11266R, Scer\GAL4^Hml.PG is a suppressor of hemocyte phenotype of Ras85D^V12.UAS, Scer\GAL4^Hml.PG

(Ashton-Beaucage et al., 2014)

CG1603^GD9160, Scer\GAL4^Hml.PG is a suppressor of hemocyte phenotype of Ras85D^V12.UAS, Scer\GAL4^Hml.PG

(Ashton-Beaucage et al., 2014)

rl^GD4697, Scer\GAL4^Hml.PG is a suppressor of hemocyte phenotype of Ras85D^V12.UAS, Scer\GAL4^Hml.PG

(Ashton-Beaucage et al., 2014)

gfzf^GD10273, Scer\GAL4^Hml.PG is a suppressor of hemocyte phenotype of Ras85D^V12.UAS, Scer\GAL4^Hml.PG

(Ashton-Beaucage et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Partially rescues

Scer\GAL4^Hml.PG/Gyc76C^UAS.Tag:MYC partially rescues Gyc76C^KG03723

(Iwashita et al., 2020)

Scer\GAL4^Hml.PG/asrij^UAS.cKa partially rescues asrij⁹

(Kulkarni et al., 2011)

Trpml^UAS.cVa/Scer\GAL4^Hml.PG partially rescues Trpml¹

(Venkatachalam et al., 2008)

Fails to rescue

Scer\GAL4^Hml.PG/Torsin^UAS.cWa fails to rescue Torsin^KO78

(Grillet et al., 2016)

Scer\GAL4^Hml.PG/G9a^UAS.cKa fails to rescue G9a^DD2

(Merkling et al., 2015)

Comments

Images (0)

Mutant

Wild-type

Stocks (6)

Bloomington

6395

w¹¹¹⁸; P{Hml-GAL4.G}5-6

6396

w¹¹¹⁸; P{Hml-GAL4.G}6-4

6397

w¹¹¹⁸; P{Hml-GAL4.G}6-4 P{UAS-GFP::lacZ.nls}15.1 P{UAS-GFP.S65T}Myo31DF^T2

Kyoto

108766

w¹¹¹⁸; P{Hml-GAL4.G}5-6

108767

w¹¹¹⁸; P{Hml-GAL4.G}6-4

108768

w¹¹¹⁸; P{Hml-GAL4.G}6-4 P{UAS-GFP::lacZ.nls}15.1 P{UAS-GFP.S65T}Myo31DF^T2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Scer\GAL4^Hml.PG

Name Synonyms

Hemolectin promoter construct of Goto

(Goto, 2001.12.6)

Secondary FlyBase IDs

References (57)

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