Allele Dmel\RhoGAP93B^{dsRNA.Scer\UAS}

General Information
Symbol	Dmel\RhoGAP93B^{dsRNA.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0128103
Feature type	allele	Associated gene	Dmel\RhoGAP93B

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - RNAi
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Billuart et al., 2001) in vitro construct - RNAi (Billuart et al., 2001)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of two copies of RhoGAP93B sequence, arranged in an inverted repeat. (Billuart et al., 2001)
Carried in construct	P{UAS-RhoGAP93B-dsRNA} (Hu et al., 2005, Billuart et al., 2001)
Cytology
Phenotypic Data
Phenotypic Class
	lethal, with Scer\GAL4^T80 (Billuart et al., 2001) lethal, with Scer\GAL4^αTub84B.PL (Billuart et al., 2001) visible, with Scer\GAL4^ey.PB (Billuart et al., 2001)
Phenotype Manifest In
	eye, with Scer\GAL4^ey.PB (Billuart et al., 2001)
Detailed Description
	Statement Reference Expression of RhoGAP93B^{dsRNA.Scer\UAS} under the control of Scer\GAL4^OK107 results in no obvious phenotype. Expression of RhoGAP93B^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ey.PB results in a reduction in the size of the eye. (Billuart et al., 2001)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhancer of
Statement Reference RhoGAP93B^{dsRNA.Scer\UAS}/Scer\GAL4^elav.PLu is an enhancer of medial longitudinal fascicle phenotype of robo⁵, sli¹ (Hu et al., 2005)
Additional Comments
Genetic Interactions
Statement Reference In robo⁵/+, sli¹/+ transheterozygous embryos, the medial longitudinal pathway occasionally crosses the midline. The addition of RhoGAP93B^{dsRNA.Scer\UAS} (driven by Scer\GAL4^elav.PLu) significantly enhances this phenotype. The subsequent addition of RhoGAP93B^Scer\UAS.cHa suppresses this enhancement. (Hu et al., 2005) When RhoGAP93B^{dsRNA.Scer\UAS} is driven by Scer\GAL4^elav.PLu in a fra^unspecified background no midline crossing defects are seen. When RhoGAP93B^{dsRNA.Scer\UAS} is driven by Scer\GAL4^elav.PLu in a Ptp69D⁴/Ptp10D^unspecified background no midline crossing defects are seen. When RhoGAP93B^{dsRNA.Scer\UAS} is driven by Scer\GAL4^elav.PLu in a Ptp69D^unspecified/Ptp10D^unspecified background no midline crossing defects are seen. (Hu, 2005)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	RhoGAP93B^{dsRNA.Scer\UAS} is rescued by RhoGAP93B^Scer\UAS.cHa/Scer\GAL4^elav.PLu (Hu et al., 2005)
Partially rescued by	RhoGAP93B^{dsRNA.Scer\UAS} is partially rescued by RhoGAP93B^Scer\UAS.cHa/Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Hu et al., 2005)
Comments
Stocks ( 2 )
Bloomington	6445 P{UAS-RhoGAP93B-dsRNA}4.1, y¹ w^* 6686 y¹ w^*; P{UAS-RhoGAP93B-dsRNA}3.1
Notes on Origin
Discoverer

Comments
	Transcription from the P{UAS-RhoGAP93B-dsRNA} construct should produce RhoGAP93B dsRNA, resulting in dsRNA interference (RNAi) of the RhoGAP93B gene. (Billuart et al., 2001)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	RhoGAP93B^{dsRNA.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Hu et al., 2005, Proc. Natl. Acad. Sci. U.S.A. 102(12): 4613--4618 Cross GTPase-activating protein (CrossGAP)/Vilse links the Roundabout receptor to Rac to regulate midline repulsion. [FBrf0191476] Billuart et al., 2001, Cell 107(2): 195--207 Regulating axon branch stability. The role of p190 RhoGAP in repressing a retraction signaling pathway. [FBrf0139610]
Supplementary material	Hu, 2005, Proc. Natl. Acad. Sci. USA 102(12): [title not yet available] [FBrf0191477]

Allele Dmel\RhoGAP93BdsRNA.Scer\UAS

Allele Dmel\RhoGAP93B^{dsRNA.Scer\UAS}