FB2025_01 , released February 20, 2025
Allele: Dmel\Rho1RNAi.UAS.cBa
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General Information
Symbol
Dmel\Rho1RNAi.UAS.cBa
Species
D. melanogaster
Name
FlyBase ID
FBal0128117
Feature type
allele
Associated gene
Dmel\Rho1
Associated Insertion(s)
Carried in Construct
P{UAS-Rho1.RNAi.B}
Also Known As
UAS-RhoAIR
Key Links
Transgenic product class
RNAi_reagent
(Billuart et al., 2001)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Billuart et al., 2001)
Mutagen
in vitro construct
(Billuart et al., 2001)
Progenitor genotype
Carried in construct
P{UAS-Rho1.RNAi.B}
Cytology
Description

UASt regulatory sequences drive expression of two copies of the entire Rho1 open reading frame, arranged in an inverted repeat.

(Billuart et al., 2001)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Rho1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Rho1dsRNA.UAS under the control of Scer\GAL4path-c135 leads to either absent or only a trace of intact carpet glia cell plasma membrane (with a negligible extension toward the anterior while remaining in the posterior center of the eye disc) and movement of retinal basal glia cells (Repo) farther anteriorly reaching the differentiating photoreceptor cells in the eye disc of the third instar larvae when compared to controls. Also it leads to impaired blood brain barrier in adults compared to controls.

      (Ho et al., 2019)

      Expression of Rho1dsRNA.Scer\UAS under the control of Scer\GAL4GMR.PU does not disrupt eye morphology in adults.

      (Minjarez et al., 2016)

      Expression of Rho1dsRNA.Scer\UAS under the control of Scer\GAL4OK107 results in the presence of multinucleated cells in the mushroom body.

      (Billuart et al., 2001)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhancer of
      NOT Enhancer of
      Suppressor of
      NOT Suppressor of
      Phenotype Manifest In
      Enhancer of
      NOT Enhancer of
      Statement
      Reference

      Rho1RNAi.UAS.cBa, Scer\GAL4hs.2sev is a non-enhancer of ommatidium phenotype of Scer\GAL4hs.2sev, nmoUAS.cUa

      (Mirkovic et al., 2011)
      Suppressor of
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The dot-like small eye phenotype characteristic for flies expressing egrScer\UAS.cMa under the control of Scer\GAL4GMR.PFa is exacerbated further by co-expression of Rho1dsRNA.Scer\UAS RNAi resulting in a 'no eye' phenotype with scar-like and necrosis-like tissue present.

      (Ruan et al., 2016)

      Coexpression of Rho1dsRNA.Scer\UAS with shgdCR3h.Scer\UAS.T:Avic\GFP-rs, under the control of Scer\GAL4hs.2sev, enhances the under-rotation of ommatidia seen in Scer\GAL4hs.2sev>shgdCR3h.Scer\UAS.T:Avic\GFP-rs eyes.

      (Mirkovic and Mlodzik, 2006)
      Xenogenetic Interactions
      Statement
      Reference

      The defective eye morphology (disrupted ommatidial lattice) observed in adult flies expressing either Hsap\MAPTScer\UAS.B or Hsap\APP2xAPP.SP.Scer\UAS under the control of Scer\GAL4GMR.PU is significantly worsened by co-expression of Rho1dsRNA.Scer\UAS RNAi with necrotic spots appearing in the posterior eye.

      (Minjarez et al., 2016)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Transcription from the Rho1dsRNA.Scer\UAS construct should produce Rho1 dsRNA, resulting in dsRNA interference (RNAi) of the Rho1 gene.

      (Billuart et al., 2001)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Rho1RNAi.UAS.cBa
      Rho1dsRNA.Scer\UAS
      Rho1dsRNA.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (17)