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General Information
Symbol
Dmel\RacGAP84CUAS.cRa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Raymond
FlyBase ID
FBal0128121
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a full length RacGAP84C cDNA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

95% of animals expressing RacGAP84CScer\UAS.cRa under the control of Scer\GAL4GMR.PU die as black pupae. Adult escapers have rough and reduced eyes with a glossy surface and a black patch at the anterior. Retinal thickness is reduced and a decreased number of misshapen ommatidia are randomly dispersed in a vacuolated matrix. Fused and mis-oriented photoreceptor cells are seen.

Embryos expressing high levels of RacGAP84CScer\UAS.cRa under the control of Scer\GAL4ptc-559.1 have cuticles consisting of only a residue of the two unfused lateral epidermis sheets and the ventral epidermis is completely missing. Embryos expressing lower levels of RacGAP84CScer\UAS.cRa under the control of Scer\GAL4ptc-559.1 show less severe defects, having an "anterior open" phenotype resulting from defective dorsal closure and lack of head involution. Small ventral holes are often also seen in these embryos. Embryos expressing RacGAP84CScer\UAS.cRa under the control of Scer\GAL4LE have an anterior open phenotype. These embryos show about 6% lethality. Embryos expressing RacGAP84CScer\UAS.cRa under the control of Scer\GAL4pnr-MD237 or Scer\GAL469B have dorsal closure defects. In stage 13 embryos expressing RacGAP84CScer\UAS.cRa under the control of Scer\GAL4ptc-559.1 most of the cells along the dorsal midline remain polygonal while some cells either widen or pinch together, individually or in groups of 2 or 3 (in contrast to wild type where cells are columnar in shape). Defects in the actin cytoskeleton are also seen in these cells.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
NOT Enhancer of
Statement
Reference
Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

In robo5/+, sli1/+ transheterozygous embryos, the medial longitudinal pathway occasionally crosses the midline. The addition of RacGAP84CScer\UAS.cRa (driven by Scer\GAL4elav.PLu) has no effect on this phenotype.

Co-expression of RacGAP84CScer\UAS.cRa suppresses the phenotype caused by expression of Rac1V12.Scer\UAS under the control of Scer\GAL4ptc-559.1 so that 1/3 of embryos have only limited cuticle defects (in the anterior region).

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
RacGAP84CScer\UAS.cRa
RacGAP84CUAS.cRa
rnRacGAPScer\UAS.cRa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Raymond
Secondary FlyBase IDs
    References (3)