FlyBase Allele Report: Dmel\tum[dsRNA.UAS.cBa]

General Information

Symbol

Dmel\tum^{dsRNA.UAS.cBa}

Species

D. melanogaster

Name

FlyBase ID

FBal0128123

Feature type

allele

Associated gene

Dmel\tum

Associated Insertion(s)

Carried in Construct

P{UAS-RacGAP50C-dsRNA}

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

in vitro construct - regulatory fusion

(Billuart et al., 2001)

in vitro construct - RNAi

(Billuart et al., 2001)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{UAS-RacGAP50C-dsRNA}

Cytology

Nature of the lesion

Statement

Reference

UAS regulatory sequences drive expression of two copies of tum sequence, arranged in an inverted repeat.

(Billuart et al., 2001)

Allele components

Product class / Tool use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

tum

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^αTub84B.PL

(Billuart et al., 2001)

neuroanatomy defective, with Scer\GAL4^ey-OK107

(Billuart et al., 2001)

partially lethal - majority die, with Scer\GAL4^T80

(Billuart et al., 2001)

visible, with Scer\GAL4^ey.PB

(Billuart et al., 2001)

wound healing defective | embryonic stage, with Scer\GAL4^{VP16.mat.αTub67C}

(Nakamura et al., 2017)

Phenotype Manifest In

adult mushroom body, with Scer\GAL4^ey-OK107

(Billuart et al., 2001)

eye, with Scer\GAL4^ey.PB

(Billuart et al., 2001)

eye & ommatidium, with Scer\GAL4^ey.PB

(D'Avino et al., 2004)

mushroom body & axon, with Scer\GAL4^ey-OK107

(Billuart et al., 2001)

ommatidium, with Scer\GAL4^ey.PB

(Billuart et al., 2001)

Detailed Description

Statement

Reference

Wounded embryos expressing tum^{dsRNA.Scer\UAS.cBa} under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} show minor repair defects compared to wounded controls: wound overexpansion and slightly delayed wound closure that is compensated for by slightly increased rates of expansion and closure; additional injection of an anti-Tum antibody did not enhance the wound overexpansion or the delay in wound healing.

(Nakamura et al., 2017)

Flies expressing tum^{dsRNA.Scer\UAS.cBa} under the control of Scer\GAL4^GMR.PFa exhibit no visible eye phenotype.

(Lee and Thomas, 2011)

Expression of RacGAP50C^{dsRNA.Scer\UAS}, under the regulation of Scer\GAL4^ey.PB in the developing eye imaginal disc reduces its size and alters the organisation and dimension of the ommatidia, as well as leading to the formation of multinucleate cells.

(D'Avino et al., 2004)

Expression of RacGAP50C^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ey.PB results in a rough eye phenotype. Expression of RacGAP50C^{dsRNA.Scer\UAS} under the control of Scer\GAL4^OK107 results in cell number reduction, abnormally large cells and axon overextension in the mushroom body.

(Billuart et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

tum^{dsRNA.UAS.cBa}/Scer\GAL4^GMR.PFa is an enhancer of visible phenotype of Scer\GAL4^GMR.PFa, kst^GD1472

(Lee and Thomas, 2011)

Suppressor of

Statement

Reference

tum^{dsRNA.UAS.cBa}/Scer\GAL4^GMR.PFa is a suppressor of visible phenotype of Scer\GAL4^GMR.PFa, kst^βH33.UAS

(Lee and Thomas, 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

tum^{dsRNA.UAS.cBa}, Scer\GAL4^ey.PB has eye & ommatidium phenotype, enhanceable by Rho1^72O/Rho1[+]

(D'Avino et al., 2004)

NOT Enhanced by

Statement

Reference

tum^{dsRNA.UAS.cBa}, Scer\GAL4^ey.PB has eye & ommatidium phenotype, non-enhanceable by sti³

(D'Avino et al., 2004)

Suppressed by

Statement

Reference

tum^{dsRNA.UAS.cBa}, Scer\GAL4^ey.PB has eye & ommatidium phenotype, suppressible by Rac2^Δ/Mtl[+]/Mtl^Δ/Rac2[+]/Rac1^J10/Rac1[+]

(D'Avino et al., 2004)

Enhancer of

Statement

Reference

tum^{dsRNA.UAS.cBa}/Scer\GAL4^GMR.PFa is an enhancer of eye phenotype of Scer\GAL4^GMR.PFa, kst^GD1472

(Lee and Thomas, 2011)

NOT Enhancer of

Statement

Reference

tum^{dsRNA.UAS.cBa} is a non-enhancer of medial longitudinal fascicle phenotype of robo1⁵, sli¹

(Hu et al., 2005)

Suppressor of

Statement

Reference

tum^{dsRNA.UAS.cBa}/Scer\GAL4^GMR.PFa is a suppressor of eye phenotype of Scer\GAL4^GMR.PFa, kst^βH33.UAS

(Lee and Thomas, 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The combined expression of both kst^GD1472 and tum^{dsRNA.Scer\UAS.cBa} via Scer\GAL4^GMR.PFa results in a synergistic interaction and significant disruption of eye development.

Co-expression of tum^{dsRNA.Scer\UAS.cBa} with kst^{βH33.Scer\UAS} under the control of Scer\GAL4^GMR.PFa suppresses the eye phenotype resulting from the expression of kst^{βH33.Scer\UAS} alone.

(Lee and Thomas, 2011)

Rac1^J10 Rac2^Δ Mtl^Δ triple mutant embryos (lacking both maternal and zygotic function of the Rac1, Rac2 and Mtl genes) are able to dominantly suppress the RacGAP50C^{dsRNA.Scer\UAS} (under the regulation of Scer\GAL4^ey.PB) eye phenotype. The rough eye phenotype associated with the expression of RacGAP50C^{dsRNA.Scer\UAS}, under the regulation of Scer\GAL4^ey.PB in the developing eye imaginal disc is dominantly enhanced by Rho1^72O. The sti³ mutation does not modify the RacGAP50C^{dsRNA.Scer\UAS} rough eye (under the regulation of Scer\GAL4^ey.PB) phenotype.

(D'Avino et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6439

y¹ w^*; P{UAS-RacGAP50C-dsRNA}1.2

Notes on Origin

Discoverer

Comments

Transcription from the P{UAS-RacGAP50C-dsRNA} construct should produce RacGAP50C dsRNA, resulting in dsRNA interference (RNAi) of the RacGAP50C gene.

(Billuart et al., 2001)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

RacGAP50C^{dsRNA.Scer\UAS}

tum^RNAi

(Nakamura et al., 2017)

tum^{dsRNA.Scer\UAS.2}

tum^{dsRNA.Scer\UAS.cBa}

tum^{dsRNA.UAS.cBa}

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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