Allele Dmel\tum^{dsRNA.Scer\UAS.cBa}

General Information
Symbol	Dmel\tum^{dsRNA.Scer\UAS.cBa}	Species	D. melanogaster
Name		FlyBase ID	FBal0128123
Feature type	allele	Created / Updated	2006-08-20/2007-09-28
Associated gene	Dmel\tum

Allele class
Mutagen	in vitro construct \| regulatory fusion, in vitro construct \| RNAi
Nature of the Allele
Allele class
Mutagen	in vitro construct \| regulatory fusion (Billuart et al., 2001) in vitro construct \| RNAi (Billuart et al., 2001)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of two copies of RacGAP50C sequence, arranged in an inverted repeat. (Billuart et al., 2001)
Assay mode	In transgenic Drosophila (intraspecific) (Billuart et al., 2001)
Carried in construct	P{UAS-RacGAP50C-dsRNA} (Billuart et al., 2001)
Cytology
Phenotypic Data
Phenotypic Class
	lethal, with Scer\GAL4^αTub84B.PL (Billuart et al., 2001) semi-lethal, with Scer\GAL4^T80 (Billuart et al., 2001) visible, with Scer\GAL4^ey.PB (Billuart et al., 2001) neuroanatomy defective, with Scer\GAL4^OK107 (Billuart et al., 2001)
Phenotype Manifest In
	eye, with Scer\GAL4^ey.PB (Billuart et al., 2001) ommatidium, with Scer\GAL4^ey.PB (Billuart et al., 2001) eye & ommatidium, with Scer\GAL4^ey.PB (D'Avino et al., 2004) mushroom body, with Scer\GAL4^OK107 (Billuart et al., 2001) mushroom body & axon, with Scer\GAL4^OK107 (Billuart et al., 2001)
Detailed Description
	Statement Reference Expression of RacGAP50C^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ey.PB results in a rough eye phenotype. Expression of RacGAP50C^{dsRNA.Scer\UAS} under the control of Scer\GAL4^OK107 results in cell number reduction, abnormally large cells and axon overextension in the mushroom body. (Billuart et al., 2001) Expression of RacGAP50C^{dsRNA.Scer\UAS}, under the regulation of Scer\GAL4^ey.PB in the developing eye imaginal disc reduces its size and alters the organisation and dimension of the ommatidia, as well as leading to the formation of multinucleate cells. (D'Avino et al., 2004)
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement Reference tum^{dsRNA.Scer\UAS.cBa}, Scer\GAL4^ey.PB has eye & ommatidium phenotype, enhanceable by Rho1^72O/Rho1[+] (D'Avino et al., 2004)
NOT Enhanced by
Statement Reference tum^{dsRNA.Scer\UAS.cBa}, Scer\GAL4^ey.PB has eye & ommatidium phenotype, non-enhanceable by sti³ (D'Avino et al., 2004)
Suppressed by
Statement Reference tum^{dsRNA.Scer\UAS.cBa}, Scer\GAL4^ey.PB has eye & ommatidium phenotype, suppressible by Rac2^Δ/Mtl[+]/Mtl^Δ/Rac2[+]/Rac1^J10/Rac1[+] (D'Avino et al., 2004)
Additional Comments
Genetic Interactions
Statement Reference Rac1^J10 Rac2^Δ Mtl^Δ triple mutant embryos (lacking both maternal and zygotic function of the Rac1, Rac2 and Mtl genes) are able to dominantly suppress the RacGAP50C^{dsRNA.Scer\UAS} (under the regulation of Scer\GAL4^ey.PB) eye phenotype. The rough eye phenotype associated with the expression of RacGAP50C^{dsRNA.Scer\UAS}, under the regulation of Scer\GAL4^ey.PB in the developing eye imaginal disc is dominantly enhanced by Rho1^72O. The sti³ mutation does not modify the RacGAP50C^{dsRNA.Scer\UAS} rough eye (under the regulation of Scer\GAL4^ey.PB) phenotype. (D'Avino et al., 2004)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	6439 y¹ w^*; P{UAS-RacGAP50C-dsRNA}1.2
Notes on Origin
Discoverer

Comments
	Transcription from the P{UAS-RacGAP50C-dsRNA} construct should produce RacGAP50C dsRNA, resulting in dsRNA interference (RNAi) of the RacGAP50C gene. (Billuart et al., 2001)
Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	RacGAP50C^{dsRNA.Scer\UAS} tum^{dsRNA.Scer\UAS.2} tum^{dsRNA.Scer\UAS.cBa}
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	D'Avino et al., 2004, J. Cell Biol. 166(1): 61--71 Mutations in sticky lead to defective organization of the contractile ring during cytokinesis and are enhanced by Rho and suppressed by Rac. [FBrf0179178] Billuart et al., 2001, Cell 107(2): 195--207 Regulating axon branch stability. The role of p190 RhoGAP in repressing a retraction signaling pathway. [FBrf0139610]
FlyBase analysis	FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]

Allele Dmel\tumdsRNA.Scer\UAS.cBa

Allele Dmel\tum^{dsRNA.Scer\UAS.cBa}