eye & ommatidium, with Scer\GAL4ey.PB
mushroom body & axon, with Scer\GAL4ey-OK107
Wounded embryos expressing tumdsRNA.Scer\UAS.cBa under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 show minor repair defects compared to wounded controls: wound overexpansion and slightly delayed wound closure that is compensated for by slightly increased rates of expansion and closure; additional injection of an anti-Tum antibody did not enhance the wound overexpansion or the delay in wound healing.
Flies expressing tumdsRNA.Scer\UAS.cBa under the control of Scer\GAL4GMR.PFa exhibit no visible eye phenotype.
Expression of RacGAP50CdsRNA.Scer\UAS, under the regulation of Scer\GAL4ey.PB in the developing eye imaginal disc reduces its size and alters the organisation and dimension of the ommatidia, as well as leading to the formation of multinucleate cells.
Expression of RacGAP50CdsRNA.Scer\UAS under the control of Scer\GAL4ey.PB results in a rough eye phenotype. Expression of RacGAP50CdsRNA.Scer\UAS under the control of Scer\GAL4OK107 results in cell number reduction, abnormally large cells and axon overextension in the mushroom body.
tumdsRNA.UAS.cBa/Scer\GAL4GMR.PFa is an enhancer of visible phenotype of Scer\GAL4GMR.PFa, kstGD1472
tumdsRNA.UAS.cBa/Scer\GAL4GMR.PFa is a suppressor of visible phenotype of Scer\GAL4GMR.PFa, kstβH33.UAS
tumdsRNA.UAS.cBa, Scer\GAL4ey.PB has eye & ommatidium phenotype, enhanceable by Rho172O/Rho1[+]
tumdsRNA.UAS.cBa, Scer\GAL4ey.PB has eye & ommatidium phenotype, non-enhanceable by sti3
tumdsRNA.UAS.cBa, Scer\GAL4ey.PB has eye & ommatidium phenotype, suppressible by Rac2Δ/Mtl[+]/MtlΔ/Rac2[+]/Rac1J10/Rac1[+]
tumdsRNA.UAS.cBa/Scer\GAL4GMR.PFa is an enhancer of eye phenotype of Scer\GAL4GMR.PFa, kstGD1472
tumdsRNA.UAS.cBa is a non-enhancer of medial longitudinal fascicle phenotype of robo15, sli1
tumdsRNA.UAS.cBa/Scer\GAL4GMR.PFa is a suppressor of eye phenotype of Scer\GAL4GMR.PFa, kstβH33.UAS
The combined expression of both kstGD1472 and tumdsRNA.Scer\UAS.cBa via Scer\GAL4GMR.PFa results in a synergistic interaction and significant disruption of eye development.
Co-expression of tumdsRNA.Scer\UAS.cBa with kstβH33.Scer\UAS under the control of Scer\GAL4GMR.PFa suppresses the eye phenotype resulting from the expression of kstβH33.Scer\UAS alone.
Rac1J10 Rac2Δ MtlΔ triple mutant embryos (lacking both maternal and zygotic function of the Rac1, Rac2 and Mtl genes) are able to dominantly suppress the RacGAP50CdsRNA.Scer\UAS (under the regulation of Scer\GAL4ey.PB) eye phenotype. The rough eye phenotype associated with the expression of RacGAP50CdsRNA.Scer\UAS, under the regulation of Scer\GAL4ey.PB in the developing eye imaginal disc is dominantly enhanced by Rho172O. The sti3 mutation does not modify the RacGAP50CdsRNA.Scer\UAS rough eye (under the regulation of Scer\GAL4ey.PB) phenotype.
Transcription from the P{UAS-RacGAP50C-dsRNA} construct should produce RacGAP50C dsRNA, resulting in dsRNA interference (RNAi) of the RacGAP50C gene.