W834term | Lar-PA; W816term | Lar-PB; W717term | Lar-PD; W837term | Lar-PF; W740term | Lar-PG; W732term | Lar-PH; W746term | Lar-PI
G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.
photoreceptor cell R7 & axon | somatic clone
photoreceptor cell R7 & growth cone | somatic clone
Expression of either trioScer\UAS.cBa or RhoGAP100FScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Act.PU in Lar2127 R7 photoreceptor cell clones partially suppresses the failure of the axon terminals to contact the M6 layer of the medulla.
When large clones of cells mutant for Liprin-αE, Lar2127, and CadNΔ14 are generated in the eye, R4 axon targeting errors are observed, but the frequency of defects is similar to Liprin-αE; Lar2127, Lar2127; CadNΔ14, or Liprin-αE; CadNΔ14 double mutant combinations.
Expression of LarScer\UAS.T:Ivir\HA1, LarC1638S.Scer\UAS, LarC1929S.Scer\UAS, LarCSX2.Scer\UAS, LarD1YLCS.Scer\UAS, LarΔIg123.Scer\UAS, LarΔFn123.Scer\UAS, LarΔFn456.Scer\UAS, or LarΔIg123ΔFn1-6.Scer\UAS.T:wg,T:Ivir\HA1 under the control of Scer\GAL4elav-C155 in LarC12/Lar2127 mutant eye discs rescues axon mistargeting. Expression of LarPPLL.Scer\UAS or LarPPLL.D1CS.Scer\UAS only partially rescues the axon targeting phenotype. Expression of LarΔFn789.Scer\UAS or LarΔFn2-9.Scer\UAS fails to rescue the axon targeting phenotype.
Expression of LarScer\UAS.T:Ivir\HA1 or LarPPLL.Scer\UAS under the control of Scer\GAL4elav-C155 in LarC12/Lar2127 mutant third instar larvae rescues synaptic growth at the neuromuscular junction. Expression of LarC1638S.Scer\UAS, LarD1YLCS.Scer\UAS, or LarCSX2.Scer\UAS only partially rescues this phenotype.
Expression of LarΔC.Scer\UAS.T:wg,T:Ivir\HA1 under the control of Scer\GAL4T155 fails to rescue the egg elongation phenotype observed in stage 14 oocytes from Lar2127/LarC12 mutants and indeed exacerbates the phenotype, with approximately 44% of the oocytes displaying a 'rounded phenotype', compared to approximately 30% in the Lar2127/LarC12 mutant.