FB2025_02 , released April 17, 2025
Allele: Dmel\LamUAS.cGa
Open Close
General Information
Symbol
Dmel\LamUAS.cGa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Guillemin
FlyBase ID
FBal0128181
Feature type
allele
Associated gene
Dmel\Lam
Associated Insertion(s)
Carried in Construct
P{UAS-Lam.G}
Also Known As
UAS-lamin Dm0
Key Links
Transgenic product class
cDNA
(Guillemin et al., 2001)
wild_type
(Guillemin et al., 2001)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Guillemin et al., 2001)
wild_type
(Guillemin et al., 2001)
Mutagen
in vitro construct
(Guillemin et al., 2001)
Progenitor genotype
Carried in construct
P{UAS-Lam.G}
Cytology
Description

UASt regulatory sequences drive expression of Lam cDNA J14-3A.

(Guillemin et al., 2001)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Lam
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of LamScer\UAS.cGa in adult longitudinal muscle cells under the control of Scer\GAL4Mhc.Switch.PO (induced by feeding RU486 to flies at the adult stages) results in a significant increase in nuclear perimeter and wrinkled, lobulated nuclei. Electron microscopy shows that these flies display nuclear phenotypes usually only observed during aging; loss of peripheral heterochromatin, separation of inner and outer nuclear membrane, accumulation of ring-like structures and a strong increase of dark material in the nucleoplasm are all observed.

      Expression of LamScer\UAS.cGa in adult muscle cells under the control of Scer\GAL4Mhc.Switch.PO (induced by feeding RU486 to flies at the adult stages) results in a 54% reduction in mean lifespan compared to controls. While the mortality rate is similar to controls, the time of onset differs. Expression in the abdominal fat body under the control of Scer\GAL4Switch1.106 reduces the mean lifespan to 46% of controls, and in this case the rate of mortality is increased compared to controls. Expression of LamScer\UAS.cGa in the nervous system under the control of Scer\GAL4elav.Switch.PO has no effect on lifespan.

      The nuclei of fat body cells expressing LamScer\UAS.cGa under the control of Scer\GAL4Switch1.106 (induced by feeding RU486 to flies at the adult stages) are larger than controls. An increase in nuclear size is also seen in neurons when LamScer\UAS.cGa is expressed in neurons under the control of Scer\GAL4elav.Switch.PO.

      Flies expressing LamScer\UAS.cGa under the control of either Scer\GAL4Mhc.Switch.PO or Scer\GAL4Switch1.106 (induced by feeding RU486 to flies at the adult stages) show an early decline in climbing ability compared to controls.

      (Brandt et al., 2008)

      Expression of LamScer\UAS.cGa under the control of Scer\GAL4GMR.PU results in eye defects: eyes are roughened or extremely deformed and loss of pigmentation is seen.

      Expression of LamScer\UAS.cGa under the control of Scer\GAL4GMR.PU results in a high degree of lethality in strong expression lines.

      (Padiath et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      Scer\GAL4c306/LamUAS.cGa partially rescues LamKK102399

      (Penfield and Montell, 2023)

      LamUAS.cGa/Scer\GAL4bs.Term partially rescues Lam04643

      (Guillemin et al., 2001)
      Comments

      Partially rescues the Lam04643 tracheal phenotype; at 29oC 56% of dorsal tracheal branches are normal in Lam04643 embryos expressing LamScer\UAS.cGa under the control of Scer\GAL4bs.Term, compared to only 16% in Lam04643 embryos.

      (Guillemin et al., 2001)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      LamScer\UAS.cGa
      LamUAS.cGa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Guillemin
      (Guillemin et al., 2001)
      Secondary FlyBase IDs
        References (8)