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General Information
Symbol
Dmel\unc-5UAS.cKa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Keleman
FlyBase ID
FBal0128973
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Unc5, UAS-unc-5
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Expression of full length unc-5 is governed by UASt regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

R7 photoreceptor axons that over-express unc-5Scer\UAS.cKa (under the control of Scer\GAL4GMR.PF) are redirected to the more superficial layer M0-1.

Misexpression of unc-5Scer\UAS.cKa throughout the PNS under the control of Scer\GAL45-40 results in disruption of sensory input patterns. Whie the sensory axons project normally within the peripheral nerves to the CNS, once they arrive they fail to grow towards the midline and tend to stall laterally, or in some cases fail to enter the CNS altogether. Despite the disruption of the sensory input, the overall structure of the CNS appears normal, although subtle secondary defects would not be detectable. Expression of unc-5Scer\UAS.cKa phenocopies the effects of inhibiting the neurons and results in propagation defects in the crawling pattern.

Both ventral nerve cord commissures are absent in every segment of embryos expressing unc-5Scer\UAS.cKa under the control of Scer\GAL41407.

The axons of sensory neurons that express Scer\GAL4ato-NP6558 normally arborise in the intermediate region of the longitudinal connective when they enter the central nervous system (CNS). When these axons are forced to express unc-5Scer\UAS.cKa via Scer\GAL4ato-NP6558, they terminate instead lateral to the connective.

When unc-5Scer\UAS.cKa is expressed in embryonic salivary glands, driven by Scer\GAL4prd.RG1, the glands become oriented laterally away from the CNS midline, instead of lying parallel to it as in wild type. This phenotype has 70% penetrance.

In the ventral nerve cord of stage 16 unc-5Scer\UAS.cKa; Scer\GAL4eg-Mz360 embryo, serotonergic neurons fail to cross the midline. In addition, as subset of these neurons lose their serotonin uptake ability. The cell bodies of serotonergic neurons in the ventral nerve cord of stage 16 unc-5Scer\UAS.cKa; Scer\GAL4eg-Mz360 embryos are shifted laterally compared to wild-type.

Embryos expressing unc-5Scer\UAS.cKa under the control of Scer\GAL4gcm.PP show a block in the medial migration of longitudinal glia and an excess of glia migrating to the lateral edge of the central nervous system and onto the nerve roots.

When expression is driven by Scer\GAL4elav.PLu commissures are completely lacking from the embryonic CNS, because the commissural axons are prevented from crossing the midline.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Both ventral nerve cord commissures are absent in every segment of embryos expressing unc-5Scer\UAS.cKa under the control of Scer\GAL41407 in a homozygous NetAΔ mutant genetic background.

Both ventral nerve cord commissures are absent in every segment of embryos expressing unc-5Scer\UAS.cKa under the control of Scer\GAL41407 in a homozygous NetBΔ mutant genetic background.

Both ventral nerve cord commissures are present in the majority of segments of embryos expressing unc-5Scer\UAS.cKa under the control of Scer\GAL41407 in a homozygous Df(1)NetABΔ mutant genetic background.

Homozygous NetAΔ has little effect on long-range repulsion of axons expressing unc-5Scer\UAS.cKa under the control of Scer\GAL4ato-NP6558.

Homozygous NetBΔ has little effect on long-range repulsion of axons expressing unc-5Scer\UAS.cKa under the control of Scer\GAL4ato-NP6558.

Homozygous Df(1)NetABΔ completely eliminates the long-range repulsion of axons expressing unc-5Scer\UAS.cKa under the control of Scer\GAL4ato-NP6558.

Short-range repulsion is preserved in NetAΔ, NetBKI.T:SV5\V5,T:Zzzz\TM,T:Hsap\MYC embryos that express unc-5Scer\UAS.cKa under the control of Scer\GAL41407, as the ventral nerve cord commissures are completely absent in every segment examined.

Short-range repulsion is preserved in NetAΔ, NetBKI.T:Hsap\MYC embryos that express unc-5Scer\UAS.cKa under the control of Scer\GAL41407, as the ventral nerve cord commissures are completely absent in every segment examined.

Long-range axon-repulsion is completely abolished in NetAΔ, NetBKI.T:SV5\V5,T:Zzzz\TM,T:Hsap\MYC embryos that express unc-5Scer\UAS.cKa under the control of Scer\GAL4ato-NP6558.

Long-range axon-repulsion is preserved in NetAΔ, NetBKI.T:Hsap\MYC embryos that express unc-5Scer\UAS.cKa under the control of Scer\GAL4ato-NP6558.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Five out of five inserts tested gave completely penetrant phenotype with single copy of construct.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
unc-5Scer\UAS.cKa
unc-5UAS.cKa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Keleman
Secondary FlyBase IDs
    References (7)