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General Information
Symbol
Dmel\slik04807b
Species
D. melanogaster
Name
disrupted innervation
FlyBase ID
FBal0129067
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
slidui, slitdui
Mutagen
    Nature of the Allele
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference

    P{lacW} insertion 29,404bp upstream of the 5' end of the sli transcript, with the Ecol\lacZ coding region oriented toward the sli locus.

    Insertion components
    P{lacW}slik04807b
    Product class / Tool use(s)
    Encoded product / tool
    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In

    eye photoreceptor cell & axon (with sli2)

    eye photoreceptor cell & axon (with sli05248)

    eye photoreceptor cell & axon (with sliE-158)

    photoreceptor cell R1 & axon

    photoreceptor cell R2 & axon

    photoreceptor cell R3 & axon

    photoreceptor cell R4 & axon

    photoreceptor cell R5 & axon

    photoreceptor cell R6 & axon

    Detailed Description
    Statement
    Reference

    slik04807b homozygosity leads to ectopic localization of inner proliferating center (IPC) neuroblasts in the medulla of third instar larvae; defects in optic lobe organization with an absence of the organization of photoreceptor cell axons in pupae; and elongated optic lobes with architecture defects (such as absence of organization of the medulla and elongated lobula plate but no changes in the organization of lobula or lamina), ectopic projections in the medulla (likely to be originated from lamina neurons), ectopic localization of IPC-derived cell populations (such as lobula plate T4/T5 neurons) misplaced in the proximal medulla and incorrect connection of the lamina to the medulla through its proximal region (next to the lobula plate) instead of connecting through the distal medulla in adults when compared to controls.

    slik04807b homozygous adults exhibit defects in response to the light compared to controls.

    slik04807b heterozygous adults do not exhibit any optic lobe neuropil defects when compared to controls.

    The adult brain of sli2/slik04807b transheterozygotes exhibits major defects in neuropil organization in several central brain areas including the central complex, mushroom body lobes, antennal lobes and Robo2/Robo3-expressing axons, as compared to either heterozygous controls; their adult sLNv neurons exhibit significantly longer axon projections compared to controls.

    The adult brain of slik04807b homozygotes exhibits defects in the organization of the ellipsoid body and the mushroom body, and their adult sLNv neurons exhibit significantly longer axon projections, as compared to controls.

    Photoreceptor cell axons do not form an optic chiasm in slik04807b mutants.

    There are gaps in the lamina neuropil in the visual system of homozygous third instar larvae and increased numbers of photoreceptor axons enter the medulla; many R2-R5 axons pass through the lamina and enter the medulla, in contrast to wild type where they stop in the lamina neuropil. slik04807b/sli05248, slik04807b/sliE-158 and slik04807b/sli2 third instar larvae show defects in photoreceptor axon targeting, with gaps in the lamina and increased numbers of axons entering the medulla. slik04807b/sli2 animals show disruption of the lamina glia layer; clumps of glia and gaps in the glial layers are seen. The regions of photoreceptor axon mistargeting correlate with areas of lamin glial disruption. Many distal cell neurons enter the base of the lamina in slik04807b/sli2 third instar larvae and some distal cell neurons invade the lamina neuropil, disrupting photoreceptor innervation.

    Mutant strongly disrupts the target layer selections of R1-R6 photoreceptor axons.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    NOT Enhancer of
    Statement
    Reference

    sli[+]/slik04807b is a non-enhancer of abnormal neuroanatomy phenotype of egh7

    Suppressor of
    NOT Suppressor of
    Statement
    Reference

    sli[+]/slik04807b is a non-suppressor of abnormal neuroanatomy phenotype of egh7

    Phenotype Manifest In
    NOT Enhancer of
    Statement
    Reference

    sli[+]/slik04807b is a non-enhancer of photoreceptor cell R1 & axon phenotype of egh7

    sli[+]/slik04807b is a non-enhancer of photoreceptor cell R2 & axon phenotype of egh7

    sli[+]/slik04807b is a non-enhancer of photoreceptor cell R3 & axon phenotype of egh7

    sli[+]/slik04807b is a non-enhancer of photoreceptor cell R4 & axon phenotype of egh7

    sli[+]/slik04807b is a non-enhancer of photoreceptor cell R5 & axon phenotype of egh7

    sli[+]/slik04807b is a non-enhancer of photoreceptor cell R6 & axon phenotype of egh7

    Suppressor of
    NOT Suppressor of
    Statement
    Reference

    sli[+]/slik04807b is a non-suppressor of photoreceptor cell R1 & axon phenotype of egh7

    sli[+]/slik04807b is a non-suppressor of photoreceptor cell R2 & axon phenotype of egh7

    sli[+]/slik04807b is a non-suppressor of photoreceptor cell R3 & axon phenotype of egh7

    sli[+]/slik04807b is a non-suppressor of photoreceptor cell R4 & axon phenotype of egh7

    sli[+]/slik04807b is a non-suppressor of photoreceptor cell R5 & axon phenotype of egh7

    sli[+]/slik04807b is a non-suppressor of photoreceptor cell R6 & axon phenotype of egh7

    Additional Comments
    Genetic Interactions
    Statement
    Reference

    The expression of robo2UAS.EGFP leads to shorter sLNv neuron projections (Scer\GAL4Pdf.PU-driven expression) and to the absence of the normal DCN neuron commissure (Scer\GAL4ato.PU-driven expression), both of which are suppressed by sli2/slik04807b transheterozygosity.

    In egh7/Y; slik04807b/+ mutants, the R-cell axonal projection pattern is defective like in egh7 single mutants. egh7/+; slik04807b/+ mutants appear wild type.

    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Images (0)
    Mutant
    Wild-type
    Stocks (1)
    Notes on Origin
    Discoverer
    Comments
    Comments

    Precise excision of the P{lacW} element reverts the mutant phenotype.

    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (4)
    Reported As
    Name Synonyms
    Secondary FlyBase IDs
      References (8)