Expression of fzScer\UAS.cSa
under the control of Scer\GAL4αTub84B.PP
in clones in the wing results in nonautonomous effects on wing margin bristle polarity proximal to the clone. Expression of fzScer\UAS.cSa
under the control of Scer\GAL4Bx-MS1096
results in defects in wing hair polarity and a few multiple wing hairs are seen.
flies have a zone of reversed polarity straddling the anterior-posterior compartment boundaries in adult abdominal segements.
flies have only minor planar polarity defects in the abdomen: some dishevelment and occasional patches of hairs in the pleura with disturbed polarity.
somatic clones in the abdominal pleura have strong effects on planar cell polarity: Up to several rows of cells in the anterior half of the clone and in front it show reversal of polarity (they point away from the clone); hairs lateral to the clone also point outwards. This phenotype is unaffected by the location of clones relative to the anterior-posterior compartment boundary. The clones behave in the same way when generated in a fz15
background except that non-autonomous effects on planar polarity only occur in cells touching the clone: the clone imposes normal polarity for about one cell behind and reversed polarity for about one cell in front.
In the abdomens of fzScer\UAS.cSa
flies trichome polarity is reversed for two to three cells on either side of the anterior-posterior compartment boundary. When these flies are also homozygous for fz15
) or fz21
), there is reversal of trichome polarity anterior to the compartment border, but it is more limited and more difficult to define (the reversal is noisy and the hairs are somewhat disheveled). In the pleura, imposed on the rather chaotic hairs seen in fz-
flies, there are two differently sized zones of oriented hairs, the smaller pointing anteriorly near the front of the posterior compartment, and the larger one pointing posteriorly behind it.