FlyBase Allele Report: Dmel\fz[UAS.cSa]

General Information

Symbol

Dmel\fz^UAS.cSa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Strapps

FlyBase ID

FBal0130285

Feature type

allele

Associated gene

Dmel\fz

Associated Insertion(s)

Carried in Construct

P{UAS-fz.S}

Also Known As

UAS-Fz, UAS.fz

Key Links

Transgenic product class

wild_type

(Strapps and Tomlinson, 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Strapps and Tomlinson, 2001)

Mutagen

in vitro construct

(Strapps and Tomlinson, 2001)

Progenitor genotype

Carried in construct

P{UAS-fz.S}

Cytology

Description

UAS regulatory sequences drive expression of fz.

(Strapps and Tomlinson, 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal planar polarity, with Scer\GAL4^Bx-MS1096

(Katanaev et al., 2005)

abnormal planar polarity, with Scer\GAL4^C-765

(Strapps and Tomlinson, 2001)

abnormal planar polarity, with Scer\GAL4^en-e16E

(Lawrence et al., 2004)

abnormal planar polarity, with Scer\GAL4^en-e16E, fz¹⁵

(Lawrence et al., 2004)

abnormal planar polarity, with Scer\GAL4^hh-Gal4

(Lawrence et al., 2004)

abnormal planar polarity, with Scer\GAL4^hh-Gal4, fz²¹

(Lawrence et al., 2004)

abnormal planar polarity, with Scer\GAL4^αTub84B.PL

(Lawrence et al., 2004)

abnormal planar polarity | cell non-autonomous | somatic clone, with Scer\GAL4^Ubx.PdC

(Lawrence et al., 2004)

abnormal planar polarity | cell non-autonomous | somatic clone, with Scer\GAL4^Ubx.PdC, fz¹⁵

(Lawrence et al., 2004)

abnormal planar polarity | cell non-autonomous | somatic clone, with Scer\GAL4^αTub84B.PP

(Katanaev et al., 2005)

abnormal planar polarity | somatic clone, with Scer\GAL4^Ubx.PdC

(Lawrence et al., 2004)

abnormal planar polarity | somatic clone, with Scer\GAL4^Ubx.PdC, fz¹⁵

(Lawrence et al., 2004)

visible, with Scer\GAL4^C-765

(Strapps and Tomlinson, 2001)

Phenotype Manifest In

ommatidium, with Scer\GAL4^unspecified

(Katanaev et al., 2005)

trichome & adult abdomen, with Scer\GAL4^hh-Gal4

(Lawrence et al., 2004)

trichome & pleural membrane, with Scer\GAL4^en-e16E

(Lawrence et al., 2004)

trichome & pleural membrane, with Scer\GAL4^en-e16E, fz¹⁵

(Lawrence et al., 2004)

trichome & pleural membrane, with Scer\GAL4^hh-Gal4

(Lawrence et al., 2004)

trichome & pleural membrane, with Scer\GAL4^hh-Gal4, fz²¹

(Lawrence et al., 2004)

trichome & pleural membrane, with Scer\GAL4^αTub84B.PL

(Lawrence et al., 2004)

trichome & pleural membrane | somatic clone, with Scer\GAL4^Ubx.PdC

(Lawrence et al., 2004)

trichome & pleural membrane | somatic clone, with Scer\GAL4^Ubx.PdC, fz¹⁵

(Lawrence et al., 2004)

trichome & pleural membrane | somatic clone | cell non-autonomous, with Scer\GAL4^Ubx.PdC

(Lawrence et al., 2004)

trichome & pleural membrane | somatic clone | cell non-autonomous, with Scer\GAL4^Ubx.PdC, fz¹⁵

(Lawrence et al., 2004)

wing hair, with Scer\GAL4^Bx-MS1096

(Katanaev et al., 2005)

wing hair, with Scer\GAL4^C-765

(Strapps and Tomlinson, 2001)

wing hair | increased number, with Scer\GAL4^Bx-MS1096

(Katanaev et al., 2005)

wing margin, with Scer\GAL4^C-765

(Strapps and Tomlinson, 2001)

wing margin bristle | cell non-autonomous | somatic clone, with Scer\GAL4^αTub84B.PP

(Katanaev et al., 2005)

Detailed Description

Statement

Reference

Expression of fz^Scer\UAS.cSa under the control of Scer\GAL4^αTub84B.PP in clones in the wing results in nonautonomous effects on wing margin bristle polarity proximal to the clone. Expression of fz^Scer\UAS.cSa under the control of Scer\GAL4^Bx-MS1096 results in defects in wing hair polarity and a few multiple wing hairs are seen.

(Katanaev et al., 2005)

fz^Scer\UAS.cSa; Scer\GAL4^hh-Gal4 flies have a zone of reversed polarity straddling the anterior-posterior compartment boundaries in adult abdominal segements.

fz^Scer\UAS.cSa; Scer\GAL4^αTub84B.PL flies have only minor planar polarity defects in the abdomen: some dishevelment and occasional patches of hairs in the pleura with disturbed polarity.

fz^Scer\UAS.cSa; Scer\GAL4^Ubx.PdC somatic clones in the abdominal pleura have strong effects on planar cell polarity: Up to several rows of cells in the anterior half of the clone and in front it show reversal of polarity (they point away from the clone); hairs lateral to the clone also point outwards. This phenotype is unaffected by the location of clones relative to the anterior-posterior compartment boundary. The clones behave in the same way when generated in a fz¹⁵ background except that non-autonomous effects on planar polarity only occur in cells touching the clone: the clone imposes normal polarity for about one cell behind and reversed polarity for about one cell in front.

In the abdomens of fz^Scer\UAS.cSa; Scer\GAL4^en-e16E or fz^Scer\UAS.cSa; Scer\GAL4^hh-Gal4 flies trichome polarity is reversed for two to three cells on either side of the anterior-posterior compartment boundary. When these flies are also homozygous for fz¹⁵ (Scer\GAL4^en-e16E) or fz²¹ (Scer\GAL4^hh-Gal4), there is reversal of trichome polarity anterior to the compartment border, but it is more limited and more difficult to define (the reversal is noisy and the hairs are somewhat disheveled). In the pleura, imposed on the rather chaotic hairs seen in fz^- flies, there are two differently sized zones of oriented hairs, the smaller pointing anteriorly near the front of the posterior compartment, and the larger one pointing posteriorly behind it.

(Lawrence et al., 2004)

Expression of fz^Scer\UAS.cSa under the control of Scer\GAL4^C-765 results in polarity defects throughout the wing blade and margin.

(Strapps and Tomlinson, 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^Bx-MS1096, fz^UAS.cSa has abnormal planar polarity phenotype, enhanceable by Gαo^UAS.cKa, Scer\GAL4^Bx-MS1096

(Katanaev et al., 2005)

Scer\GAL4^hh-Gal4, fz^UAS.cSa has abnormal planar polarity phenotype, enhanceable by pk^pk-sple-13/pk^pk-sple-13

(Lawrence et al., 2004)

Suppressed by

Statement

Reference

Scer\GAL4^αTub84B.PL, fz^UAS.cSa has abnormal planar polarity | somatic clone | cell non-autonomous phenotype, suppressible by Vang^stbm-6/Vang^stbm-6

(Lawrence et al., 2004)

Scer\GAL4^ptc-559.1, fz^UAS.cSa has abnormal planar polarity phenotype, suppressible by stan^E59/stan³

(Lawrence et al., 2004)

Enhancer of

Statement

Reference

fz^UAS.cSa, Scer\GAL4^Bx-MS1096 is an enhancer of abnormal planar polarity phenotype of Gαo^UAS.cKa, Scer\GAL4^Bx-MS1096

(Katanaev et al., 2005)

Suppressor of

Statement

Reference

fz^UAS.cSa/Scer\GAL4^αTub84B.PL is a suppressor | somatic clone of abnormal planar polarity | somatic clone | cell non-autonomous phenotype of pwn^unspecified, stan^E59

(Lawrence et al., 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^Bx-MS1096, fz^UAS.cSa has wing hair | increased number phenotype, enhanceable by Gαo^UAS.cKa, Scer\GAL4^Bx-MS1096

(Katanaev et al., 2005)

Scer\GAL4^Bx-MS1096, fz^UAS.cSa has wing hair phenotype, enhanceable by Gαo^UAS.cKa, Scer\GAL4^Bx-MS1096

(Katanaev et al., 2005)

fz^UAS.cSa has trichome & adult abdomen phenotype, enhanceable by pk^pk-sple-13/pk^pk-sple-13

(Lawrence et al., 2004)

Suppressed by

Statement

Reference

Scer\GAL4^unspecified, fz^UAS.cSa has ommatidium phenotype, suppressible | partially by Bper\ptxA^UAS.cKa, Scer\GAL4^unspecified

(Katanaev et al., 2005)

Scer\GAL4^αTub84B.PL, fz^UAS.cSa has trichome & adult abdomen | somatic clone | cell non-autonomous phenotype, suppressible by Vang^stbm-6/Vang^stbm-6

(Lawrence et al., 2004)

Scer\GAL4^ptc-559.1, fz^UAS.cSa has trichome & adult abdomen phenotype, suppressible by stan^E59/stan³

(Lawrence et al., 2004)

Enhancer of

Statement

Reference

fz^UAS.cSa, Scer\GAL4^Bx-MS1096 is an enhancer of wing hair | increased number phenotype of Gαo^UAS.cKa, Scer\GAL4^Bx-MS1096

(Katanaev et al., 2005)

fz^UAS.cSa, Scer\GAL4^Bx-MS1096 is an enhancer of wing hair phenotype of Gαo^UAS.cKa, Scer\GAL4^Bx-MS1096

(Katanaev et al., 2005)

Suppressor of

Statement

Reference

fz^UAS.cSa/Scer\GAL4^αTub84B.PL is a suppressor | somatic clone of trichome & adult abdomen | somatic clone | cell non-autonomous phenotype of pwn^unspecified, stan^E59

(Lawrence et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

G-oα47A^Scer\UAS.cKa and fz^Scer\UAS.cSa act synergistically when co-expressed using Scer\GAL4^Bx-MS1096, resulting in severe defects in wing hair polarity and many cells with multiple wing hairs.

(Katanaev et al., 2005)

When fz^Scer\UAS.cSa; Scer\GAL4^hh-Gal4 flies are pk^pk-sple-13 homozygous, the zone of repolarisation straddling the anterior-posterior compartment boundaries in adult abdominal segements is enlarged, but otherwise unchanged. (Polarity is normal in this region in pk^pk-sple-13/pk^pk-sple-13 flies.)

The non-autonomous effects of somatic clones of fz^Scer\UAS.cSa; Scer\GAL4^αTub84B.PL cells (created using Scer\GAL80 MARCM system) on cell-polarity in the adult abdomen are suppressed by Vang^stbm-6/Vang^stbm-6.

The planar repolarisation seen in the adult abdomen of fz^Scer\UAS.cSa; Scer\GAL4^ptc-559.1 adults is suppressed by stan^E59/stan³.

(Lawrence et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

fz^Scer\UAS.cSa

(Casal et al., 2006)

fz^UAS.cSa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Strapps

(Strapps and Tomlinson, 2001)

Secondary FlyBase IDs

References (6)

Report Sections

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