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General Information
Symbol
Dmel\fzUAS.cSa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Strapps
FlyBase ID
FBal0130285
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Fz, UAS.fz
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UAS regulatory sequences drive expression of fz.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
trichome & adult abdomen, with Scer\GAL4hh-Gal4
trichome & pleural membrane, with Scer\GAL4en-e16E
trichome & pleural membrane, with Scer\GAL4en-e16E, fz15
trichome & pleural membrane, with Scer\GAL4hh-Gal4
trichome & pleural membrane, with Scer\GAL4hh-Gal4, fz21
trichome & pleural membrane, with Scer\GAL4αTub84B.PL
trichome & pleural membrane | somatic clone, with Scer\GAL4Ubx.PdC
trichome & pleural membrane | somatic clone, with Scer\GAL4Ubx.PdC, fz15
trichome & pleural membrane | somatic clone | cell non-autonomous, with Scer\GAL4Ubx.PdC
trichome & pleural membrane | somatic clone | cell non-autonomous, with Scer\GAL4Ubx.PdC, fz15
Detailed Description
Statement
Reference
Expression of fzScer\UAS.cSa under the control of Scer\GAL4αTub84B.PP in clones in the wing results in nonautonomous effects on wing margin bristle polarity proximal to the clone. Expression of fzScer\UAS.cSa under the control of Scer\GAL4Bx-MS1096 results in defects in wing hair polarity and a few multiple wing hairs are seen.
fzScer\UAS.cSa; Scer\GAL4hh-Gal4 flies have a zone of reversed polarity straddling the anterior-posterior compartment boundaries in adult abdominal segements. fzScer\UAS.cSa; Scer\GAL4αTub84B.PL flies have only minor planar polarity defects in the abdomen: some dishevelment and occasional patches of hairs in the pleura with disturbed polarity. fzScer\UAS.cSa; Scer\GAL4Ubx.PdC somatic clones in the abdominal pleura have strong effects on planar cell polarity: Up to several rows of cells in the anterior half of the clone and in front it show reversal of polarity (they point away from the clone); hairs lateral to the clone also point outwards. This phenotype is unaffected by the location of clones relative to the anterior-posterior compartment boundary. The clones behave in the same way when generated in a fz15 background except that non-autonomous effects on planar polarity only occur in cells touching the clone: the clone imposes normal polarity for about one cell behind and reversed polarity for about one cell in front. In the abdomens of fzScer\UAS.cSa; Scer\GAL4en-e16E or fzScer\UAS.cSa; Scer\GAL4hh-Gal4 flies trichome polarity is reversed for two to three cells on either side of the anterior-posterior compartment boundary. When these flies are also homozygous for fz15 (Scer\GAL4en-e16E) or fz21 (Scer\GAL4hh-Gal4), there is reversal of trichome polarity anterior to the compartment border, but it is more limited and more difficult to define (the reversal is noisy and the hairs are somewhat disheveled). In the pleura, imposed on the rather chaotic hairs seen in fz- flies, there are two differently sized zones of oriented hairs, the smaller pointing anteriorly near the front of the posterior compartment, and the larger one pointing posteriorly behind it.
Expression of fzScer\UAS.cSa under the control of Scer\GAL4C-765 results in polarity defects throughout the wing blade and margin.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Suppressed by
Statement
Reference
Scer\GAL4αTub84B.PL, fzUAS.cSa has trichome & adult abdomen | somatic clone | cell non-autonomous phenotype, suppressible by Vangstbm-6/Vangstbm-6
Scer\GAL4ptc-559.1, fzUAS.cSa has trichome & adult abdomen phenotype, suppressible by stanE59/stan3
Enhancer of
Suppressor of
Statement
Reference
fzUAS.cSa/Scer\GAL4αTub84B.PL is a suppressor | somatic clone of trichome & adult abdomen | somatic clone | cell non-autonomous phenotype of pwnunspecified, stanE59
Additional Comments
Genetic Interactions
Statement
Reference
G-oα47AScer\UAS.cKa and fzScer\UAS.cSa act synergistically when co-expressed using Scer\GAL4Bx-MS1096, resulting in severe defects in wing hair polarity and many cells with multiple wing hairs.
When fzScer\UAS.cSa; Scer\GAL4hh-Gal4 flies are pkpk-sple-13 homozygous, the zone of repolarisation straddling the anterior-posterior compartment boundaries in adult abdominal segements is enlarged, but otherwise unchanged. (Polarity is normal in this region in pkpk-sple-13/pkpk-sple-13 flies.) The non-autonomous effects of somatic clones of fzScer\UAS.cSa; Scer\GAL4αTub84B.PL cells (created using Scer\GAL80 MARCM system) on cell-polarity in the adult abdomen are suppressed by Vangstbm-6/Vangstbm-6. The planar repolarisation seen in the adult abdomen of fzScer\UAS.cSa; Scer\GAL4ptc-559.1 adults is suppressed by stanE59/stan3.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
fzScer\UAS.cSa
fzUAS.cSa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Strapps
Secondary FlyBase IDs
    References (5)