FlyBase Allele Report: Dmel\Myc[PL35]

General Information

Symbol

Dmel\Myc^PL35

Species

D. melanogaster

Name

FlyBase ID

FBal0130425

Feature type

allele

Associated gene

Dmel\Myc

Associated Insertion(s)

P{lacW}Myc^PL35

Carried in Construct

Also Known As

dm^PL35, dmyc^PL35, PL35

Key Links

Allele class

hypomorphic allele - molecular evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

hypomorphic allele - molecular evidence

(Benassayag et al., 2005)

Mutagen

Delta2-3 transposase

(Bourbon et al., 2002)

Progenitor genotype

Associated Insertion(s)

P{lacW}Myc^PL35

Cytology

Description

Insertion in the second intron.

(Benassayag et al., 2005)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

ommatidium

(Bellosta et al., 2005)

wing disc

(Benassayag et al., 2005)

Detailed Description

Statement

Reference

The number and size of ommatidia in dm^PL35 eye somatic clones is reduced compared to wild-type.

(Bellosta et al., 2005)

Hemizygous males die as pharate adults of reduced size, with a thin bristle phenotype. Development is delayed compared to wild-type controls.

The wing discs of mutant third instar larvae are reduced in size by approximately 30% relative to wild type, due to a reduction in cell size.

Homozygous clones are on average only approximately 40% of the size of their wild-type twin spot.

(Benassayag et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Myc^PL35 has decreased cell number | somatic clone phenotype, enhanceable by pont^unspecified

(Bellosta et al., 2005)

Myc^PL35 has decreased cell size | somatic clone phenotype, enhanceable by pont^unspecified

(Bellosta et al., 2005)

Myc^PL35 has decreased cell number | somatic clone phenotype, enhanceable by rept^unspecified

(Bellosta et al., 2005)

Myc^PL35 has decreased cell size | somatic clone phenotype, enhanceable by rept^unspecified

(Bellosta et al., 2005)

Suppressed by

Statement

Reference

Myc^PL35 has lethal phenotype, suppressible by Hsap\MYC^hs.1

(Benassayag et al., 2005)

Myc^PL35 has lethal phenotype, suppressible by Hsap\MYC^hs.2

(Benassayag et al., 2005)

Suppressor of

Statement

Reference

Myc^PL35/Myc^αTub84B.PBb, Scer\GAL4^αTub84B.PP is a suppressor of increased cell size | somatic clone phenotype of Scer\GAL4^αTub84B.PP, yki^UAS.cHa

(Ziosi et al., 2010)

Other

Statement

Reference

Myc^αTub84B.PBb, Myc^PL35, Scer\GAL4^αTub84B.PP, yki^UAS.cHa has decreased cell size phenotype

(Ziosi et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Myc^PL35 has ommatidium | somatic clone phenotype, enhanceable by pont^unspecified

(Bellosta et al., 2005)

Myc^PL35 has ommatidium | somatic clone phenotype, enhanceable by rept^unspecified

(Bellosta et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Heterozygosity for pont^unspecified in dm^PL35 eye somatic clones results in a decrease in the number and size of ommatidia compared to dm^PL35 eye somatic clones. Heterozygosity for rept^unspecified in dm^PL35 eye somatic clones results in a slight decrease in the number and size of ommatidia compared to dm^PL35 eye somatic clones. Heterozygosity for rept^unspecified and pont^unspecified in dm^PL35 eye somatic clones results in a decrease in the number and size of ommatidia compared to dm^PL35 eye somatic clones in a wild-type background and in rept^unspecified or pont^unspecified heterozygous backgrounds.

(Bellosta et al., 2005)

Xenogenetic Interactions

Statement

Reference

Expression of either Hsap\MYC^hs.1 or Hsap\MYC^hs.2 allows a few adult dm^PL35 males to survive.

(Benassayag et al., 2005)

Complementation and Rescue Data

Rescued by

Myc^PL35 is rescued by Myc^hs.PSA

(Benassayag et al., 2005)

Comments

dm^hs.PSA rescues the lethality of dm^PL35 mutants.

(Benassayag et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Re-mobilisation of the transposon causes the lethality phenotype to be lost.

(Bourbon et al., 2002)

Precise excision of the insertion reverts the lethal phenotype.

(Benassayag et al., 2005)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Myc^PL35

dm^PL35

(Baumgardt et al., 2014, Neto-Silva et al., 2010, Schwinkendorf and Gallant, 2009)

dmyc^PL35

(Ziosi et al., 2010, Rhiner et al., 2009, Benassayag et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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