Allele Dmel\Stat92E³⁹⁷

General Information
Symbol	Dmel\Stat92E³⁹⁷	Species	D. melanogaster
Name		FlyBase ID	FBal0130586
Feature type	allele	Associated gene	Dmel\Stat92E
Also Known As	stat³⁹⁷

Map ( GBrowse )
Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Silver and Montell, 2001)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 3R:16,365,494..16,365,494 na_change=G16365494A comment=G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation (exact site of mutation unspecified). evidence=experimental pr_change=W594\|Stat92E-PE,W594\|Stat92E-PF,W594\|Stat92 E-PC,W468\|Stat92E-PB,W468@\|Stat92E-PG reported_na_change=G?A reported_pr_change=W594@ (Silver and Montell, 2001)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: W594@. Nucleotide substitution: G?A. (Silver and Montell, 2001)
Cytology
Phenotypic Data
Phenotypic Class
	hyperplasia \| dominant \| third instar larval stage (Gao et al., 2009) increased cell death \| conditional \| third instar larval stage (Betz et al., 2008) lethal \| embryonic stage (with Df(3R)H-B79) (Ekas et al., 2010) lethal \| first instar larval stage (with Df(3R)H-B79) (Ekas et al., 2010) visible \| somatic clone (Ekas et al., 2006, Ekas et al., 2010)
Phenotype Manifest In
	border follicle cell (Silver and Montell, 2001) border follicle cell (with Stat92E^EP3391), with Scer\GAL4[-]: (Silver et al., 2005) embryonic/larval lymph gland \| third instar larval stage (Gao et al., 2009) eye \| somatic clone (Ekas et al., 2006, Ekas et al., 2010) plasmatocyte \| third instar larval stage (Gao et al., 2009) wing disc \| conditional \| third instar larval stage (Betz et al., 2008) wing disc \| third instar larval stage (Betz et al., 2008)
Detailed Description
	Statement Reference 30% of Stat92E[397]/Df(3R)H-B79 animals die during embryogenesis and the remainder die during the first larval instar. Mosaic animals in which the eye-antennal disc is almost completely homozygous for Stat92E[397] show a reduced eye phenotype. (Ekas et al., 2010) Stat92E[397]/+ third instar larval lymph glands show hyperplasia and increased plasmatocyte differentiation. There is no marked increase in lamellocyte differentiation. (Gao et al., 2009) Stat92E[397] wing disc clones average 6.4% of the wing pouch area, compared to 28% for wild-type clones. When wing discs are subjected to x-irradiation (5,000 rad daily for 3 days), Stat92E[397] clones take up 1.1% of the total pouch area. The level of apoptosis observed is increased in mutant wing discs subjected to x-irradiation compared to controls. (Betz et al., 2008) Adults with Stat92E³⁹⁷ clones in a Minute background have aberrantly small eyes. (Ekas et al., 2006) In Stat92E^EP3391/Stat92E³⁹⁷ egg chambers, border cell migration is reduced but border cell number is not affected. (Silver et al., 2005) Homozygous mutant mosaic egg chambers display severe border cell migration defects. Border cells frequently fail to penetrate into the nurse cell clusters and can be found at the anterior tip or along the sides of the egg chamber. Alternatively the cells migrate partially, but fail top reach the oocyte by stage 10. (Silver and Montell, 2001)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Stat92E³⁹⁷ has increased cell death \| conditional \| third instar larval stage phenotype, suppressible by Scer\GAL4^tub.PU/th^Scer\UAS.cBa (Betz et al., 2008)
Phenotype Manifest In
Suppressed by
Statement Reference Stat92E³⁹⁷ has wing disc \| conditional \| third instar larval stage phenotype, suppressible by Scer\GAL4^tub.PU/th^Scer\UAS.cBa (Betz et al., 2008)
Additional Comments
Genetic Interactions
Statement Reference When th[Scer\UAS.cBa] is over-expressed in the wing disc under the control of Scer\GAL4[tub.PU] from first instar larval stage (by heat-shock induction to inactivate Scer\GAL80[αTub84B.PL]), Stat92E[397] mutant clones take up 7.7% of the wing pouch after x-irradiation treatment (5,000 rads daily for 3 days). Low levels of cell death are observed. (Betz et al., 2008)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	Stat92E^56D3 (Silver and Montell, 2001) Stat92E^80A7 (Silver and Montell, 2001) Stat92E^85C9 (Silver and Montell, 2001) Stat92E⁰⁶³⁴⁶ (Silver and Montell, 2001)
Rescued by	Stat92E³⁹⁷ is rescued by Stat92E^{Scer\UAS.P\T.T:Ivir\HA1}/Scer\GAL4^ey.PH (Ekas et al., 2010) Stat92E³⁹⁷ is rescued by Stat92E^{ΔC.Scer\UAS.P\T.T:Ivir\HA1}/Scer\GAL4^ey.PH (Ekas et al., 2010) Stat92E³⁹⁷ is rescued by Stat92E^{ΔN.Scer\UAS.P\T.T:Ivir\HA1}/Scer\GAL4^ey.PH (Ekas et al., 2010)
Not rescued by	Stat92E³⁹⁷ is not rescued by Stat92E^{R442P.Scer\UAS.P\T.T:Ivir\HA1}/Scer\GAL4^ey.PH (Ekas et al., 2010) Stat92E³⁹⁷ is not rescued by Stat92E^{Y711F.Scer\UAS.P\T.T:Ivir\HA1}/Scer\GAL4^ey.PH (Ekas et al., 2010)
Comments	The mutant eye phenotype seen in mosaic animals in which the eye-antennal disc is almost completely homozygous for Stat92E[397] is rescued by Stat92E[Scer\UAS.P\T.T:Ivir\HA1], Stat92E[ΔN.Scer\UAS.P\T.T:Ivir\HA1] or Stat92E[ΔC.Scer\UAS.P\T.T:Ivir\HA1] expressed under the control of Scer\GAL4[ey.PH]. The mutant eye phenotype seen in mosaic animals in which the eye-antennal disc is almost completely homozygous for Stat92E[397] is not rescued by Stat92E[Y711F.Scer\UAS.P\T.T:Ivir\HA1] or Stat92E[R442P.Scer\UAS.P\T.T:Ivir\HA1] expressed under the control of Scer\GAL4[ey.PH]. (Ekas et al., 2010) Stat92E³⁹⁷ fails to complement Stat92E^56D3, Stat92E^80A7 and Stat92E^85C9 with respect to lethality. (Silver and Montell, 2001)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	stat92E³⁹⁷ (Henriksen et al., 2002, Ekas et al., 2006, Flaherty et al., 2009, Betz et al., 2008) Stat92E³⁹⁷ (Ekas et al., 2010) stat^397-6 (Gao et al., 2009) stat³⁹⁷ (Silver et al., 2005, Krzemień et al., 2007)
Name Synonym
Secondary FlyBase IDs

References ( 9 )
Research paper	Ekas et al., 2010, Dev. Biol. 344(2): 621--636 Characterization of a dominant-active STAT that promotes tumorigenesis in Drosophila. [FBrf0211400] Flaherty et al., 2009, Dev. Dyn. 238(9): 2235--2253 Genome-wide expression profiling in the Drosophila eye reveals unexpected repression of notch signaling by the JAK/STAT pathway. [FBrf0208590] Gao et al., 2009, Mol. Cell. Biol. 29(22): 6086--6096 Upregulation of the Drosophila friend of GATA Gene u-shaped by JAK/STAT signaling maintains lymph gland prohemocyte potency. [FBrf0208909] Betz et al., 2008, Proc. Natl. Acad. Sci. U.S.A. 105(37): 13805--13810 STAT92E is a positive regulator of Drosophila inhibitor of apoptosis 1 (DIAP/1) and protects against radiation-induced apoptosis. [FBrf0205996] Krzemień et al., 2007, Nature 446(7133): 325--328 Control of blood cell homeostasis in Drosophila larvae by the posterior signalling centre. [FBrf0194113] Ekas et al., 2006, Development 133(23): 4721--4729 JAK/STAT signaling promotes regional specification by negatively regulating wingless expression in Drosophila. [FBrf0193730] Silver et al., 2005, Development 132(15): 3483--3492 Requirement for JAK/STAT signaling throughout border cell migration in Drosophila. [FBrf0187483] Henriksen et al., 2002, Genes Dev. 16(18): 2379--2389 Negative regulation of STAT92E by an N-terminally truncated STAT protein derived from an alternative promoter site. [FBrf0152001] Silver and Montell, 2001, Cell 107(7): 831--841 Paracrine Signaling through the JAK/STAT Pathway Activates Invasive Behavior of Ovarian Epithelial Cells in Drosophila. [FBrf0141417]

Allele Dmel\Stat92E397

Allele Dmel\Stat92E³⁹⁷